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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林順福 | |
dc.contributor.author | Kuan-Hsiu Chen | en |
dc.contributor.author | 陳冠秀 | zh_TW |
dc.date.accessioned | 2021-06-17T08:25:39Z | - |
dc.date.available | 2024-08-16 | |
dc.date.copyright | 2019-08-16 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-12 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74236 | - |
dc.description.abstract | 雄不稔性及柱頭外露率為生產雜交種水稻之重要性狀,本研究目的為選拔兩系雜交種用之稉稻、秈稻雄不稔品系及含有恢復基因的優良品系。本試驗以具溫敏性雄不稔特性之台大品系A與台南13號雜交,並將其F2族群栽種於台大農場溫室以進行性狀調查及遺傳分析。即先以182株F2品系及80組SSR和InDel分子標誌建立連鎖圖譜,並配合外表型資料以多重區間定位法定位數量性狀基因座,所分析的性狀包含溫敏性雄不稔、柱頭外露率、抽穗期、株高、葉鞘顏色、柱頭顏色、稃尖顏色、穗數、穗長和稔實率,依分析結果定位此10個性狀的QTL。本研究初步測定位於第8對染色體上的分子標誌CH0866及SLS188與溫敏性雄不稔基因緊密連鎖;此外,於第3、5、6及12對染色體上定位到調控柱頭外露率的基因;亦於第1對染色體定位到調控葉鞘顏色之基因。高柱頭外露率特性將有助於提高雜交授粉成功率,而葉鞘顏色則可應用於成功雜交苗的選別。本試驗除了初步探討溫敏性雄不稔性狀之遺傳機制外,並依據雄不稔外表型、父本稉稻背景基因型貢獻率及柱頭外露率選拔優良品系,最後推薦5個雄不稔稉稻品系、5個雄不稔秈稻品系及4個可能含有恢復基因之高產品系,供未來兩系雜交稻之研究及育種利用。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:25:39Z (GMT). No. of bitstreams: 1 ntu-108-R06621117-1.pdf: 1426733 bytes, checksum: 60fca4b14124dcf5eb034f48a3e6d7ea (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員檢定書 i
致謝 ii 中文摘要 iii Abstract iv 目錄 v 表目錄 viii 圖目錄 ix 附圖目錄 x 前言 1 前人研究 3 一、水稻雄不稔在育種上的應用 3 二、細胞質雄不稔 (CMS) 3 三、環境敏感性雄不稔 (EGMS) 4 四、水稻柱頭外露率對異交率的影響 5 五、水稻柱頭外露的基因定位 6 六、研究目的 7 材料與方法 8 一、試驗材料 8 二、外表型調查 11 (一) 量的性狀 (quantitative traits) 11 (二) 質的性狀 (qualitative traits) 11 三、基因型分析 12 (一) 水稻葉部DNA萃取 12 (二) DNA分子標誌引子合成 12 (三) 聚合酶連鎖反應及電泳分析 13 四、資料分析 14 (一) 外表型性狀相關性分析 14 (二) 建立物理圖譜與遺傳圖譜之建立 14 (三) 數量性狀基因座 (QTL) 遺傳分析 15 結果 16 一、建立分子標誌連鎖圖譜 16 二、外表型相關性分析 18 (一) 雜交相關性狀 18 (二) 產量構成要素 18 (三) 其他重要農藝性狀 18 三、數量性狀基因座定位 22 (一) 雜交相關性狀 22 (二) 產量構成要素 29 (三) 其他重要農藝性狀 32 四、優良品系之推薦 41 討論 43 一、建立連鎖圖譜 43 二、數量性狀基因座定位 44 (一) 雜交相關基因 44 (二) 產量構成要素 46 (三) 其他重要農藝性狀 46 三、優良品系選拔 49 四、目前面臨的育種難題 49 (一) 影響溫敏性雄不稔表現之環境因子 49 (二) 影響柱頭外露率的環境因子 50 結論 51 附錄 52 參考文獻 53 | |
dc.language.iso | zh-TW | |
dc.title | 生產稉稻兩系雜交種用之雄不稔品系的選育 | zh_TW |
dc.title | Selection of Male Sterile Lines for Producing Two-Line Hybrids of Japonica Rice | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳志文,賴明信,陳榮坤 | |
dc.subject.keyword | 兩系雄不稔,柱頭外露率,葉鞘顏色,分子輔助選拔, | zh_TW |
dc.subject.keyword | two-line male sterility,stigma exsertion rate,purple leaf sheath,marker-assisted selection, | en |
dc.relation.page | 62 | |
dc.identifier.doi | 10.6342/NTU201903268 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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