請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7422
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周綠蘋 | zh_TW |
dc.contributor.advisor | en | |
dc.contributor.author | 鄭丞斌 | zh_TW |
dc.contributor.author | Cheng-Pin Cheng | en |
dc.date.accessioned | 2021-05-19T17:43:19Z | - |
dc.date.available | 2024-02-28 | - |
dc.date.copyright | 2018-10-09 | - |
dc.date.issued | 2018 | - |
dc.date.submitted | 2002-01-01 | - |
dc.identifier.citation | 1. Devarajan, P., Review: neutrophil gelatinase-associated lipocalin: a troponin-like biomarker for human acute kidney injury. Nephrology (Carlton), 2010. 15(4): p. 419-28.
2. Acute renal failure.pdf. 3. fundamentalsofnursingblog. Three subtypes of AKI. December 15, 2016; Available from: https://fundamentalsofnursingblog.wordpress.com/2016/12/15/acute-kidney-injury/. 4. Osmosis, Prerenal AKI. 2016. 5. Osmosis, Intrarenal AKI. 2016. 6. Osmosis, Postrenal AKI. 2016. 7. Postrenal AKI (Causes). Available from: https://en.wikipedia.org/wiki/Acute_kidney_injury. 8. Lopes, J.A. and S. Jorge, The RIFLE and AKIN classifications for acute kidney injury: a critical and comprehensive review. Clin Kidney J, 2013. 6(1): p. 8-14. 9. Park, W.Y., et al., The risk factors and outcome of acute kidney injury in the intensive care units. Korean J Intern Med, 2010. 25(2): p. 181-7. 10. Susan M. Dirkes, B., MSA, CCRN. Acute kidney injury: Causes, phases, and early detection. 2015; Available from: https://www.americannursetoday.com/acute-kidney-injury/. 11. Parikh, C.R., et al., Urinary IL-18 is an early predictive biomarker of acute kidney injury after cardiac surgery. Kidney Int, 2006. 70(1): p. 199-203. 12. Xu, Y., et al., L-FABP: A novel biomarker of kidney disease. Clin Chim Acta, 2015. 445: p. 85-90. 13. Vanmassenhove, J., et al., Urinary and serum biomarkers for the diagnosis of acute kidney injury: an in-depth review of the literature. Nephrol Dial Transplant, 2013. 28(2): p. 254-73. 14. Mishra, J., et al., Amelioration of ischemic acute renal injury by neutrophil gelatinase-associated lipocalin. J Am Soc Nephrol, 2004. 15(12): p. 3073-82. 15. Clerico, A., et al., Neutrophil gelatinase-associated lipocalin (NGAL) as biomarker of acute kidney injury: a review of the laboratory characteristics and clinical evidences. Clin Chem Lab Med, 2012. 50(9): p. 1505-17. 16. Schmidt-Ott, K.M., et al., Dual action of neutrophil gelatinase-associated lipocalin. J Am Soc Nephrol, 2007. 18(2): p. 407-13. 17. The Neutrophil Lipocalin NGAL Is a Bacteriostatic Agent that Interferes with Siderophore-Mediated Iron Acquisition.pdf. 18. Chakraborty, S., et al., The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Biochim Biophys Acta, 2012. 1826(1): p. 129-69. 19. Supavekin, S., et al., Differential gene expression following early renal ischemia/reperfusion. Kidney Int, 2003. 63(5): p. 1714-24. 20. Yuen, P.S., et al., Ischemic and nephrotoxic acute renal failure are distinguished by their broad transcriptomic responses. Physiol Genomics, 2006. 25(3): p. 375-86. 21. 1289.full.pdf. 22. Low-cost and Ultra-sensitive Poly-Si Nanowire Biosensor for Hepatitis B Virus (HBV) DNA Detection.pdf. 23. Zheng, G., et al., Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol, 2005. 23(10): p. 1294-301. 24. A fully integrated hepatitis B virus DNA detection SoC based on monolithic polysilicon nanowire CMOS process.pdf. 25. A-CMOS-Based-Polysilicon-Nanowire-Biosensor-for-Monitoring-the-Cardiovascular-Disease-Markers-in-Human-Serum.pdf. 26. Pei-Wen, Y., et al., A device design of an integrated CMOS poly-silicon biosensor-on-chip to enhance performance of biomolecular analytes in serum samples. Biosens Bioelectron, 2014. 61: p. 112-8. 27. Chu, C.J., et al., Improving nanowire sensing capability by electrical field alignment of surface probing molecules. Nano Lett, 2013. 13(6): p. 2564-9. 28. Huang, C.W., et al., A CMOS wireless biomolecular sensing system-on-chip based on polysilicon nanowire technology. Lab Chip, 2013. 13(22): p. 4451-9. 29. Chen, K.-I., B.-R. Li, and Y.-T. Chen, Silicon nanowire field-effect transistor-based biosensors for biomedical diagnosis and cellular recording investigation. Nano Today, 2011. 6(2): p. 131-154. 30. Zhu, M., M.Z. Lerum, and W. Chen, How to prepare reproducible, homogeneous, and hydrolytically stable aminosilane-derived layers on silica. Langmuir, 2012. 28(1): p. 416-23. 31. Lu, N., et al., Ultrasensitive Detection of Dual Cancer Biomarkers with Integrated CMOS-Compatible Nanowire Arrays. Anal Chem, 2015. 87(22): p. 11203-8. 32. Lei, Y.M., et al., Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor. Biosens Bioelectron, 2017. 91: p. 1-7. 33. Han, Y., et al., Surface activation of thin silicon oxides by wet cleaning and silanization. Thin Solid Films, 2006. 510(1-2): p. 175-180. 34. Mu, L., et al., Silicon Nanowire Field-Effect Transistors—A Versatile Class of Potentiometric Nanobiosensors. IEEE Access, 2015. 3: p. 287-302. 35. Shen, M.Y., B.R. Li, and Y.K. Li, Silicon nanowire field-effect-transistor based biosensors: from sensitive to ultra-sensitive. Biosens Bioelectron, 2014. 60: p. 101-11. 36. Nicholas, M.P., L. Rao, and A. Gennerich, Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays. Methods Mol Biol, 2014. 1136: p. 137-69. 37. Son, H.W., et al., A strategy to minimize the sensing voltage drift error in a transistor biosensor with a nanoscale sensing gate. Int J Nanomedicine, 2017. 12: p. 2951-2956. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7422 | - |
dc.description.abstract | zh_TW | |
dc.description.abstract | Acute kidney injury (AKI) is condition of rapid or abrupt decline in renal function. Overall, the mortality of AKI is 20%. Serum creatinine and urine output are used to define the prognosis of AKI. However, both indicators are not fast enough to detect AKI. Increase of serum creatinine occurred within 1 to 3 days after AKI; and it is estimated that greater than 50% of kidney function must be lost before serum creatinine rises. Consequently, serum creatinine does not reflect the true decrease of glomerular filtration rate in the acute setting. On the other hand, indicators such as neutrophil gelatinase-associated lipocalin (NGAL) and liver-type fatty acid binding protein increased at an early stage. Generally, the average area under the receiver-operating characteristic curve (AUC-ROC) of predicting AKI by NGAL was 0.79; and NGAL level arises and achieves their peak level arises within four hours after surgeries[1]. Conventional enzyme-linked immunosorbent assay (ELISA) is time-consuming. In order to perform real-time detection of AKI, we developed a fast-detecting biochip using silicon nanowire field-effect transistor (SiNW FET) which can reduce the detection time to less than 30 minutes.
In this study, we first successfully purified NGAL recombinant protein and well established NGAL ELISA, which was used as a standard method to compare with. Next, we determined the coating protocol and fIn this study, we first successfully purified NGAL recombinant protein and well established NGAL ELISA, which was used as a standard method to compare with. Next, we determined the coating protocol and found that in the hydroxylation process, cholic acid had the best performance in r square and drifting rate among H2O2, cholic acid and NaOH. In the silanization process, we found that AEAPTES was less prone to self-react than APTES, which was commonly used beforeound that in the hydroxylation process, cholic acid had the best performance in r square and drifting rate among H2O2, cholic acid and NaOH. In the silanization process, we found that AEAPTES was less prone to self-react than APTES, which was commonly used before. By observing and measuring the current change, we could know the protein level within 30 minutes. In this study, 7 AKI and 5 non-AKI samples were quantified. NGAL levels of AKI patients quantified by this system ranged from 1500 to 4000 ng/mL, while levels of four non-AKI samples were 0 ng/mL. By using the SiNW-FET system, AKI and non-AKI samples can be discriminated significantly (p value<0.001). In conclusion, we developed a fast detecting biochip that can be applied to clinical quantification which is able to discriminate AKI and non-AKI samples. | en |
dc.description.provenance | Made available in DSpace on 2021-05-19T17:43:19Z (GMT). No. of bitstreams: 1 ntu-107-R05442022-1.pdf: 4472708 bytes, checksum: 3db4c9105436022f78cd4267d9ab1fc7 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 謝誌 i
摘要 iii Abstract v CONTENTS vii LIST OF TABLES AND FIGURES x Chapter 1. Introduction 1 1.1 Acute kidney injury 1 1.1.1 Prerenal AKI 2 1.1.2 Intrarenal AKI 2 1.1.3 Postrenal AKI 3 1.2 Staging and Prognosis 3 1.3 Novel Biomarkers 6 1.4 Neutrophil gelatinase-association lipocalin 8 1.5 NGAL in acute kidney injury 9 1.6 Silicon Nanowire Field-Effect Transistor (SiNW-FET) Biochip 10 1.7 Specific aim 12 Chapter 2. Materials and Methods 14 2.1 Recombinant protein expression and purification 14 2.2 Immunoblotting analysis 15 2.3 Protein identification by LC-MS/MS 15 2.3.1 In-gel digestion 15 2.3.2 LC-MS/MS analysis 17 2.3.3 Bioinformatics-Mascot Daemon 17 2.4 Quantification of NGAL of AKI and non-AKI patients’ urine by ELISA 18 2.5 Design of Si-NW Based Biosensor 19 2.6 Surface modification and functionalization 20 2.7 Quantification of NGAL of AKI patients and non-AKI control urine by Si-NW FET biochip 21 2.8 Statistical analysis 22 Chapter 3. Results 23 3.1 Purification of recombinant protein 23 3.2 Establishment of ELISA 23 3.3 Surface functionization of SiNW-FET biochip 24 3.4 Establishment of measurement protocol and standard curve 25 3.5 NGAL level quantification of AKI and non-AKI samples 27 3.6 Conclusion 28 Chapter 4. Discussion 29 Chapter 5. Tables and Figures 34 References 44 Attachments 47 | - |
dc.language.iso | en | - |
dc.title | 利用矽奈米線場效應電晶體開發以嗜中性白血球明膠酶相關運載蛋白為指標的急性腎臟損傷快速診斷晶片 | zh_TW |
dc.title | Development of NGAL fast biochip using Silicon Nanowire Field-Effect Transistors for Acute Kidney Injury Detection | en |
dc.type | Thesis | - |
dc.date.schoolyear | 106-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 林致廷;吳允升 | zh_TW |
dc.contributor.oralexamcommittee | ;; | en |
dc.subject.keyword | 急性腎臟損傷,嗜中性白血球明膠?相關運載蛋白,矽奈米線場效應電晶體,膽酸, | zh_TW |
dc.subject.keyword | AKI,NGAL,SiNW-FET,cholic acid, | en |
dc.relation.page | 74 | - |
dc.identifier.doi | 10.6342/NTU201803988 | - |
dc.rights.note | 未授權 | - |
dc.date.accepted | 2018-08-20 | - |
dc.contributor.author-college | 醫學院 | - |
dc.contributor.author-dept | 生物化學暨分子生物學研究所 | - |
dc.date.embargo-lift | 2023-10-09 | - |
顯示於系所單位: | 生物化學暨分子生物學科研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-2.pdf 目前未授權公開取用 | 4.37 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。