請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74006
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭貽生(Yi-Sheng Cheng) | |
dc.contributor.author | Hsuan-Yu Huang | en |
dc.contributor.author | 黃暄友 | zh_TW |
dc.date.accessioned | 2021-06-17T08:16:33Z | - |
dc.date.available | 2024-08-20 | |
dc.date.copyright | 2019-08-20 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-14 | |
dc.identifier.citation | 古森本. (2008). 生質能源作物之開發與潛力. 農業生技產業季刊 13, 46-53.
吳耿東. (2010). 流體化床與生質能. 科學發展 450, 20-25. 吳耿東、李宏台. (2004). 生質能源化腐朽為能源. 科學發展 383, 21-27. 陳志祐. (2010). 綠竹中與初級細胞壁生合成相關之纖維素合成酶基因探討. 博士論文 劉秀美、蔡馥嚀. (2010). 農業廢棄物生產木質分解酵素之研究. 農業生技產業季刊 24, 53-58. 蘇美惠. (2017). 全球生質能源產業與技術發展動態與趨勢分析. 台灣能資源永續與低碳經濟學會. Arioli, T., Peng, L., Betzner, A.S., Burn, J., Wittke, W., Herth, W., Camilleri, C., Hofte, H., Plazinski, J., Birch, R., Cork, A., Glover, J., Redmond, J., and Williamson, R.E. (1998). Molecular analysis of cellulose biosynthesis in Arabidopsis. Science 279, 717-720. Atanassov, II, Pittman, J.K., and Turner, S.R. (2009). Elucidating the mechanisms of assembly and subunit interaction of the cellulose synthase complex of Arabidopsis secondary cell walls. The Journal of Biological Chemistry 284, 3833-3841. Bach, H., Mazor, Y., Shaky, S., Shoham-Lev, A., Berdichevsky, Y., Gutnick, D.L., and Benhar, I. (2001). Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies. Journal of Molecular Biology 312, 79-93. Baroja-Fernández, E., Muñoz, F.J., Li, J., Bahaji, A., Almagro, G., Montero, M., Etxeberria, E., Hidalgo, M., Sesma, M.T., and Pozueta-Romero, J. (2012). Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production. Proceedings of the National Academy of Sciences 109, 321-326. Baskin, T.I. (2001). On the alignment of cellulose microfibrils by cortical microtubules: a review and a model. Protoplasma 215, 150-171. Benfey, P.N., Linstead, P.J., Roberts, K., Schiefelbein, J.W., Hauser, M.-T., and Aeschbacher, R.A. (1993). Root development in Arabidopsis: four mutants with dramatically altered root morphogenesis. Development 119, 57-70. Bessueille, L., Sindt, N., Guichardant, M., Djerbi, S., Teeri, T.T., and Bulone, V. (2009). Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis. Biochemical Journal 420, 93-103. Blackburn, M.R., Hubbard, C., Kiessling, V., Bi, Y., Kloss, B., Tamm, L.K., and Zimmer, J. (2018). Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life. Glycobiology 28, 108-121. Boyer, J.S. (2009). Evans review: cell wall biosynthesis and the molecular mechanism of plant enlargement. Functional Plant Biology 36, 383-394. Bringmann, M., Li, E., Sampathkumar, A., Kocabek, T., Hauser, M.-T., and Persson, S. (2012). POM-POM2/cellulose synthase interacting1 is essential for the functional association of cellulose synthase and microtubules in Arabidopsis. The Plant Cell 24, 163-177. Burn, J.E., Hocart, C.H., Birch, R.J., Cork, A.C., and Williamson, R.E. (2002). Functional analysis of the cellulose synthase genes CesA1, CesA2, and CesA3 in Arabidopsis. Plant Physiology 129, 797-807. Cai, G., Faleri, C., Del Casino, C., Emons, A.M.C., and Cresti, M. (2011). Distribution of callose synthase, cellulose synthase, and sucrose synthase in tobacco pollen tube is controlled in dissimilar ways by actin filaments and microtubules. Plant Physiology 155, 1169-1190. Carpita, N.C. (2012). Progress in the biological synthesis of the plant cell wall: new ideas for improving biomass for bioenergy. Current Opinion in Biotechnology 23, 330-337. Carroll, A., Mansoori, N., Li, S., Lei, L., Vernhettes, S., Visser, R.G., Somerville, C., Gu, Y., and Trindade, L.M. (2012). Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis plants. Plant Physiology 160, 726-737. Carroll, A., and Specht, C.D. (2011). Understanding plant cellulose synthases through a comprehensive investigation of the cellulose synthase family sequences. Frontiers in Plant Science 2, 5. Chen, C.Y., Hsieh, M.H., Yang, C.C., Lin, C.S., and Wang, A.Y. (2010a). Analysis of the cellulose synthase genes associated with primary cell wall synthesis in Bambusa oldhamii. Phytochemistry 71, 1270-1279. Chen, S.L., Ehrhardt, D.W., and Somerville, C.R. (2010b). Mutations of cellulose synthase (CesA1) phosphorylation sites modulate anisotropic cell expansion and bidirectional mobility of cellulose synthase. Proceedings of the National Academy of Sciences 107, 17188-17193. Cho, S.H., Purushotham, P., Fang, C., Maranas, C., Diaz-Moreno, S.M., Bulone, V., Zimmer, J., Kumar, M., and Nixon, B.T. (2017). Synthesis and self-assembly of cellulose microfibrils from reconstituted cellulose synthase. Plant Physiology 175, 146-156. Cho, S.H., Du, J., Sines, I., Poosarla, V.G., Vepachedu, V., Kafle, K., Park, Y.B., Kim, S.H., Kumar, M., and Nixon, B.T. (2015). In vitro synthesis of cellulose microfibrils by a membrane protein from protoplasts of the non-vascular plant Physcomitrella patens. Biochemical Journal 470, 195-205. Cifuentes, C., Bulone, V., and Emons, A.M. (2010). Biosynthesis of callose and cellulose by detergent extracts of tobacco cell membranes and quantification of the polymers synthesized in vitro. Journal of Integrative Plant Biology 52, 221-233. Coleman, H.D., Yan, J., and Mansfield, S.D. (2009). Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure. Proceedings of the National Academy of Sciences 106, 13118-13123. Cosgrove, D. (2005). Growth of the plant cell wall. Nature Reviews Molecular Cell Biology 6, 850. Creux, N.M., Ranik, M., Berger, D.K., and Myburg, A.A. (2008). Comparative analysis of orthologous cellulose synthase promoters from Arabidopsis, Populus and Eucalyptus: evidence of conserved regulatory elements in angiosperms. New Phytologist 179, 722-737. Crowell, E.F., Bischoff, V., Desprez, T., Rolland, A., Stierhof, Y.-D., Schumacher, K., Gonneau, M., Höfte, H., and Vernhettes, S. (2009). Pausing of Golgi bodies on microtubules regulates secretion of cellulose synthase complexes in Arabidopsis. The Plant Cell 21, 1141-1154. Crowell, E.F., Gonneau, M., Stierhof, Y.D., Hofte, H., and Vernhettes, S. (2010). Regulated trafficking of cellulose synthases. Current Opinion in Plant Biology 13, 700-705. Desprez, T., Juraniec, M., Crowell, E.F., Jouy, H., Pochylova, Z., Parcy, F., Hofte, H., Gonneau, M., and Vernhettes, S. (2007). Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana. Proceedings of the National Academy of Sciences 104, 15572-15577. Ding, S.Y., and Himmel, M.E. (2006). The maize primary cell wall microfibril: a new model derived from direct visualization. Journal of Agricultural and Food Chemistry 54, 597-606. Djerbi, S., Aspeborg, H., Nilsson, P., Sundberg, B., Mellerowicz, E., Blomqvist, K., and Teeri, T.T. (2004). Identification and expression analysis of genes encoding putative cellulose synthases (CesA) in the hybrid aspen, Populus tremula (L.)× P. tremuloides (Michx.). Cellulose 11, 301-312. Djerbi, S., Lindskog, M., Arvestad, L., Sterky, F., and Teeri, T.T. (2005). The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes. Planta 221, 739-746. Doblin, M.S., Kurek, I., Jacob-Wilk, D., and Delmer, D.P. (2002). Cellulose biosynthesis in plants: from genes to rosettes. Plant Cell Physiology 43, 1407-1420. Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792-1797. Endler, A., Kesten, C., Schneider, R., Zhang, Y., Ivakov, A., Froehlich, A., Funke, N., and Persson, S. (2015). A mechanism for sustained cellulose synthesis during salt stress. Cell 162, 1353-1364. Fox, J.D., and Waugh, D.S. (2003). Maltose-binding protein as a solubility enhancer. In E. coli Gene Expression Protocols (Springer), pp. 99-117. Fujii, S., Hayashi, T., and Mizuno, K. (2010). Sucrose synthase is an integral component of the cellulose synthesis machinery. Plant and Cell Physiology 51, 294-301. Guindon, S., and Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696-704. Gutierrez, R., Lindeboom, J.J., Paredez, A.R., Emons, A.M.C., and Ehrhardt, D.W. (2009). Arabidopsis cortical microtubules position cellulose synthase delivery to the plasma membrane and interact with cellulose synthase trafficking compartments. Nature Cell Biology 11, 797. Harholt, J., Suttangkakul, A., and Scheller, H.V. (2010). Biosynthesis of pectin. Plant Physiology 153, 384-395. Herth, W. (1983). Arrays of plasma-membrane 'rosettes' involved in cellulose microfibril formation of Spirogyra. Planta 159, 347-356. Hill, J.L., Hammudi, M.B., and Tien, M. (2014). The Arabidopsis cellulose synthase complex: a proposed hexamer of CesA trimers in an equimolar stoichiometry. The Plant Cell 26, 4834-4842. Holland, N., Holland, D., Helentjaris, T., Dhugga, K.S., Xoconostle-Cazares, B., and Delmer, D.P. (2000). A comparative analysis of the plant cellulose synthase (CesA) gene family. Plant Physiology 123, 1313-1324. Jacob-Wilk, D., Kurek, I., Hogan, P., and Delmer, D.P. (2006). The cotton fiber zinc-binding domain of cellulose synthase A1 from Gossypium hirsutum displays rapid turnover in vitro and in vivo. Proceedings of the National Academy of Sciences 103, 12191-12196. Kim, W.-C., Kim, J.-Y., Ko, J.-H., Kang, H., and Han, K.-H. (2014). Identification of direct targets of transcription factor MYB46 provides insights into the transcriptional regulation of secondary wall biosynthesis. Plant Molecular Biology 85, 589-599. Kim, W.C., Ko, J.H., Kim, J.Y., Kim, J., Bae, H.J., and Han, K.H. (2013). MYB 46 directly regulates the gene expression of secondary wall‐associated cellulose synthases in A rabidopsis. The Plant Journal 73, 26-36. Kimura, S., Laosinchai, W., Itoh, T., Cui, X., Linder, C.R., and Brown, R.M., Jr. (1999). Immunogold labeling of rosette terminal cellulose-synthesizing complexes in the vascular plant Vigna angularis. The Plant Cell 11, 2075-2086. Klemm, D., Heublein, B., Fink, H.P., and Bohn, A. (2005). Cellulose: fascinating biopolymer and sustainable raw material. Angewandte Chemie International Edition 44, 3358-3393. Korepanova, A., Moore, J., Nguyen, H., Hua, Y., Cross, T., and Gao, F. (2007). Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein. Protein Expression and Purification 53, 24-30. Kubicki, J.D., Yang, H., Sawada, D., O’Neill, H., Oehme, D., and Cosgrove, D. (2018). The shape of native plant cellulose microfibrils. Scientific Reports 8, 13983. Kubo, M., Udagawa, M., Nishikubo, N., Horiguchi, G., Yamaguchi, M., Ito, J., Mimura, T., Fukuda, H., and Demura, T. (2005). Transcription switches for protoxylem and metaxylem vessel formation. Genes Development 19, 1855-1860. Kumar, M., and Turner, S. (2015). Plant cellulose synthesis: CesA proteins crossing kingdoms. Phytochemistry 112, 91-99. Kumar, M., Wightman, R., Atanassov, I., Gupta, A., Hurst, C.H., Hemsley, P.A., and Turner, S. (2016). S-Acylation of the cellulose synthase complex is essential for its plasma membrane localization. Science 353, 166-169. Kurek, I., Kawagoe, Y., Jacob-Wilk, D., Doblin, M., and Delmer, D. (2002). Dimerization of cotton fiber cellulose synthase catalytic subunits occurs via oxidation of the zinc-binding domains. Proceedings of the National Academy of Sciences 99, 11109-11114. Lai-Kee-Him, J., Chanzy, H., Muller, M., Putaux, J.L., Imai, T., and Bulone, V. (2002). In vitro versus in vivo cellulose microfibrils from plant primary wall synthases: structural differences. The Journal of Biological Chemistry 277, 36931-36939. Lane, D.R., Wiedemeier, A., Peng, L., Höfte, H., Vernhettes, S., Desprez, T., Hocart, C.H., Birch, R.J., Baskin, T.I., and Burn, J.E. (2001). Temperature-sensitive alleles of RSW2 link the KORRIGAN endo-1,4-β-glucanase to cellulose synthesis and cytokinesis in Arabidopsis. Plant Physiology 126, 278-288. Lei, L., Singh, A., Bashline, L., Li, S., Yingling, Y.G., and Gu, Y. (2015). CELLULOSE SYNTHASE INTERACTIVE1 is required for fast recycling of cellulose synthase complexes to the plasma membrane in Arabidopsis. The Plant Cell 27, 2926-2940. Li, S., Lei, L., Somerville, C.R., and Gu, Y. (2012). Cellulose synthase interactive protein 1 (CSI1) links microtubules and cellulose synthase complexes. Proceedings of the National Academy of Sciences 109, 185-190. Liebminger, E., Grass, J., Altmann, F., Mach, L., and Strasser, R. (2013). Characterizing the link between glycosylation state and enzymatic activity of the endo-β1,4-glucanase KORRIGAN1 from Arabidopsis thaliana. Journal of Biological Chemistry 288, 22270-22280. Liu, L., Shang-Guan, K., Zhang, B., Liu, X., Yan, M., Zhang, L., Shi, Y., Zhang, M., Qian, Q., and Li, J. (2013). Brittle Culm1, a COBRA-like protein, functions in cellulose assembly through binding cellulose microfibrils. PLoS Genetics 9, e1003704. McFarlane, H.E., Doring, A., and Persson, S. (2014). The cell biology of cellulose synthesis. Annual Review of Plant Biology 65, 69-94. McNamara, J.T., Morgan, J.L., and Zimmer, J. (2015). A molecular description of cellulose biosynthesis. Annual Review of Biochemistry 84, 895-921. Moon, R.J., Martini, A., Nairn, J., Simonsen, J., and Youngblood, J. (2011). Cellulose nanomaterials review: structure, properties and nanocomposites. Chemical Society Reviews 40, 3941-3994. Morgan, J.L., Strumillo, J., and Zimmer, J. (2013). Crystallographic snapshot of cellulose synthesis and membrane translocation. Nature 493, 181. Morgan, J.L., McNamara, J.T., Fischer, M., Rich, J., Chen, H.M., Withers, S.G., and Zimmer, J. (2016). Observing cellulose biosynthesis and membrane translocation in crystallo. Nature 531, 329-334. Morgan, J.L., McNamara, J.T., and Zimmer, J. (2014). Mechanism of activation of bacterial cellulose synthase by cyclic di-GMP. Nature Structural and Molecular Biology 21, 489-496. Mueller, S.C., and Brown, R.M. (1980). Evidence for an intramembrane component associated with a cellulose microfibril-synthesizing complex in higher plants. The Journal of Cell Biology 84, 315-326. Mueller, S.C., Brown, R.M., and Scott, T.K. (1976). Cellulosic microfibrils: nascent stages of synthesis in a higher plant cell. Science 194, 949-951. Newman, R.H., Hill, S.J., and Harris, P.J. (2013). Wide-angle x-ray scattering and solid-state nuclear magnetic resonance data combined to test models for cellulose microfibrils in mung bean cell walls. Plant Physiology 163, 1558-1567. Nishiyama, Y., Langan, P., and Chanzy, H. (2002). Crystal structure and hydrogen-bonding system in cellulose Iβ from synchrotron X-ray and neutron fiber diffraction. Journal of the American Chemical Society 124, 9074-9082. Nishiyama, Y., Sugiyama, J., Chanzy, H., and Langan, P. (2003). Crystal structure and hydrogen bonding system in cellulose Iα from synchrotron X-ray and neutron fiber diffraction. Journal of the American Chemical Society 125, 14300-14306. Nixon, B.T., Mansouri, K., Singh, A., Du, J., Davis, J.K., Lee, J.G., Slabaugh, E., Vandavasi, V.G., O'Neill, H., Roberts, E.M., Roberts, A.W., Yingling, Y.G., and Haigler, C.H. (2016). Comparative structural and computational analysis supports eighteen cellulose synthases in the plant cellulose synthesis complex. Scientific Reports 6, 28696. Nuhse, T.S., Stensballe, A., Jensen, O.N., and Peck, S.C. (2004). Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database. The Plant Cell 16, 2394-2405. Omasits, U., Ahrens, C.H., Muller, S., and Wollscheid, B. (2014). Protter: interactive protein feature visualization and integration with experimental proteomic data. Bioinformatics 30, 884-886. O'sullivan, A.C. (1997). Cellulose: the structure slowly unravels. Cellulose 4, 173-207. Olek, A.T., Rayon, C., Makowski, L., Kim, H.R., Ciesielski, P., Badger, J., Paul, L.N., Ghosh, S., Kihara, D., Crowley, M., Himmel, M.E., Bolin, J.T., and Carpita, N.C. (2014). The structure of the catalytic domain of a plant cellulose synthase and its assembly into dimers. The Plant Cell 26, 2996-3009. Paredez, A.R., Somerville, C.R., and Ehrhardt, D.W. (2006). Visualization of cellulose synthase demonstrates functional association with microtubules. Science 312, 1491-1495. Pear, J.R., Kawagoe, Y., Schreckengost, W.E., Delmer, D.P., and Stalker, D.M. (1996). Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. Proceedings of the National Academy of Sciences 93, 12637-12642. Peng, L., Kawagoe, Y., Hogan, P., and Delmer, D. (2002). Sitosterol-beta-glucoside as primer for cellulose synthesis in plants. Science 295, 147-150. Persson, S., Paredez, A., Carroll, A., Palsdottir, H., Doblin, M., Poindexter, P., Khitrov, N., Auer, M., and Somerville, C.R. (2007). Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis. Proceedings of the National Academy of Sciences 104, 15566-15571. Pettolino, F.A., Walsh, C., Fincher, G.B., and Bacic, A. (2012). Determining the polysaccharide composition of plant cell walls. Nature Protocol 7, 1590-1607. Polko, J.K., and Kieber, J.J. (2019). The regulation of cellulose biosynthesis in plants. The Plant Cell 31, 282-296. Purushotham, P., Cho, S.H., Diaz-Moreno, S.M., Kumar, M., Nixon, B.T., Bulone, V., and Zimmer, J. (2016). A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro. Proceedings of the National Academy of Sciences 113, 11360-11365. Ranik, M., and Myburg, A.A. (2006). Six new cellulose synthase genes from Eucalyptus are associated with primary and secondary cell wall biosynthesis. Tree Physiology 26, 545-556. Reiss, H.D., Schnepf, E., and Herth, W. (1984). The plasma membrane of the Funaria caulonema tip cell: morphology and distribution of particle rosettes, and the kinetics of cellulose synthesis. Planta 160, 428-435. Richmond, T. (2000). Higher plant cellulose synthases. Genome Biology 1, reviews3001. Roudier, F., Fernandez, A.G., Fujita, M., Himmelspach, R., Borner, G.H., Schindelman, G., Song, S., Baskin, T.I., Dupree, P., and Wasteneys, G.O. (2005). COBRA, an Arabidopsis extracellular glycosyl-phosphatidyl inositol-anchored protein, specifically controls highly anisotropic expansion through its involvement in cellulose microfibril orientation. The Plant Cell 17, 1749-1763. Rushton, P.S., Olek, A.T., Makowski, L., Badger, J., Steussy, C.N., Carpita, N.C., and Stauffacher, C.V. (2017). Rice cellulose synthaseA8 plant-conserved region is a coiled-coil at the catalytic core entrance. Plant Physiology 173, 482-494. Salnikov, V.V., Grimson, M.J., Delmer, D.P., and Haigler, C.H. (2001). Sucrose synthase localizes to cellulose synthesis sites in tracheary elements. Phytochemistry 57, 823-833. Scavuzzo‐Duggan, T.R., Chaves, A.M., Singh, A., Sethaphong, L., Slabaugh, E., Yingling, Y.G., Haigler, C.H., and Roberts, A.W. (2018). Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated. Journal of Integrative Plant Biology 60, 481-497. Scheible, W.-R., Eshed, R., Richmond, T., Delmer, D., and Somerville, C. (2001). Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants. Proceedings of the National Academy of Sciences 98, 10079-10084. Scheller, H.V., and Ulvskov, P. (2010). Hemicelluloses. Annual Review of Plant Biology 61, 263-289. Somerville, C. (2006). Cellulose synthesis in higher plants. Annual Review of Cell and Developmental Biology 22, 53-78. Su, J.C. (1969). Carbohydrate metabolism in the shoots of bamboo Leleba oldhamii. VII. Changes of polysaccharides constituents accompanied with the growth of the plants. Journal of the Chinese Agricultural Chemical Society 10, special issue: 16-20. Taylor, N.G. (2007). Identification of cellulose synthase AtCesA7 (IRX3) in vivo phosphorylation sites-a potential role in regulating protein degradation. Plant Molecular Biology 64, 161-171. Taylor, N.G., Howells, R.M., Huttly, A.K., Vickers, K., and Turner, S.R. (2003). Interactions among three distinct CesA proteins essential for cellulose synthesis. Proceedings of the National Academy of Sciences 100, 1450-1455. Taylor, N.G., Laurie, S., and Turner, S.R. (2000). Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis. The Plant Cell 12, 2529-2540. Taylor, N.G., Scheible, W.-R., Cutler, S., Somerville, C.R., and Turner, S.R. (1999). The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis. The Plant Cell 11, 769-779. Timmers, J., Vernhettes, S., Desprez, T., Vincken, J.P., Visser, R.G., and Trindade, L.M. (2009). Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall. FEBS Letters 583, 978-982. Turner, S., and Kumar, M. (2018). Cellulose synthase complex organization and cellulose microfibril structure. Philosophical Transactions of The Royal Society A 376. Vain, T., Crowell, E.F., Timpano, H., Biot, E., Desprez, T., Mansoori, N., Trindade, L.M., Pagant, S., Robert, S., and Höfte, H. (2014). The cellulase KORRIGAN is part of the cellulose synthase complex. Plant Physiology 165, 1521-1532. Vandavasi, V.G., Putnam, D.K., Zhang, Q., Petridis, L., Heller, W.T., Nixon, B.T., Haigler, C.H., Kalluri, U., Coates, L., Langan, P., Smith, J.C., Meiler, J., and O'Neill, H. (2016). A structural study of CesA1 catalytic domain of arabidopsis cellulose synthesis complex: evidence for CesA trimers. Plant Physiology 170, 123-135. Vanholme, R., Demedts, B., Morreel, K., Ralph, J., and Boerjan, W. (2010). Lignin biosynthesis and structure. Plant Physiology 153, 895-905. Watanabe, Y., Schneider, R., Barkwill, S., Gonzales-Vigil, E., Hill, J.L., Samuels, A.L., Persson, S., and Mansfield, S.D. (2018). Cellulose synthase complexes display distinct dynamic behaviors during xylem transdifferentiation. Proceedings of the National Academy of Sciences 115, E6366-E6374. Wightman, R., and Turner, S. (2010). Trafficking of the plant cellulose synthase complex. Plant Physiology 153, 427-432. Xi, W., Song, D., Sun, J., Shen, J., and Li, L. (2017). Formation of wood secondary cell wall may involve two type cellulose synthase complexes in Populus. Plant Molecular Biology 93, 419-429. Xiong, W., Ding, Z., and Li, Y. (1980). Intercalary meristem and internodal elongation of bamboo plants. Scientia Silvae Sinicae 16, 81-89. Yamaguchi, M., Goué, N., Igarashi, H., Ohtani, M., Nakano, Y., Mortimer, J.C., Nishikubo, N., Kubo, M., Katayama, Y., and Kakegawa, K. (2010). VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system. Plant Physiology 153, 906-914. Young, C.L., Britton, Z.T., and Robinson, A.S. (2012). Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications. Biotechnology Journal 7, 620-634. Zhang, X., Dominguez, P.G., Kumar, M., Bygdell, J., Miroshnichenko, S., Sundberg, B., Wingsle, G., and Niittylä, T. (2018). Cellulose synthase stoichiometry in aspen differs from Arabidopsis and Norway spruce. Plant Physiology 177, 1096-1107. Zhong, R., Richardson, E.A., and Ye, Z.-H. (2007). The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis. The Plant Cell 19, 2776-2792. Zhu, X., Li, S., Pan, S., Xin, X., and Gu, Y. (2018). CSI1, PATROL1, and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane. Proceedings of the National Academy of Sciences 115, E3578-E3587. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74006 | - |
dc.description.abstract | 纖維素是世界上最大量的生質物,年生成量約1兆5千億噸。植物纖維素在初級細胞壁含量約占30%,次級細胞壁則約為55%。纖維素由纖維素合成酶複合體 (Cellulose synthase complex, CSC) 合成,CSC係由數個纖維素合成酶 (Cellulose synthases, CesAs) 於細胞膜上組成,纖維素合成酶複合體的組成結構及其纖維素合成的機制仍不清楚,原因在於纖維素合成酶屬膜蛋白質,第一步就是要取得大量表達及純化的蛋白質。本研究以異源表現的方式純化六種綠竹 (Bambusa oldhamii) 纖維素合成酶 (BoCesA1, 2, 3, 4, 5, 7) ,並分析其酵素活性。分別將Maltose-binding protein (MBP) 序列以及BoCesA序列構築在pYES2/CT表現載體上,並透過酵母菌細胞 (BCY123) 在醱酵槽內大量培養及誘導蛋白質表現。分別使用固定化金屬離子親和層析 (Immobilized metal affinity chromatography) 純化MBP-BoCesAs以及粒徑排阻層析 (Size-exclusion chromatography) 分離多聚體BoCesAs,自120 g酵母菌可純化約7.2 mg的BoCesA蛋白質。在電子顯微鏡的觀察下,可以觀察到多聚體BoCesAs加入基質UDP-Glucose後所生產的纖維素產物,並使用泛甲基醣醇乙酸 (Partially methylated alditol acetate) 衍生化配合氣相層析質譜儀 (Gas chromatography-mass spectrometry) 鑑定纖維素產物的鍵結為β-1,4-glucan。本研究建立了一套大量表現以及純化纖維素合成酶的流程,並確定所純化的蛋白質具有酵素活性,此酵素將可用於纖維素生合成相關研究以及蛋白質結構與功能分析。 | zh_TW |
dc.description.abstract | Cellulose is the most abundant biomass in the world with an estimated 1500 billion tons produced by plants per year. About 30% of primary cell wall and 55% of secondary cell wall consist of cellulose in plant cells. Cellulose is synthesized by the cellulose synthase complex (CSC) which contains several cellulose synthases (CesAs) in the plasma membrane. The molecular structure of CSC and the mechanism of cellulose synthesis by CesAs in plant cells remain unclear. Since CesAs are membrane proteins, the first step is to overexpress and purify a large quantity of CesAs protein. In this study, the overexpression and purification procedure for 6 genes of cellulose synthase (BoCesA1, 2, 3, 4, 5, 7) from Bambusa oldhamii were established. The Maltose-binding protein (MBP) -tagged BoCesAs were cloned into pYES2/CT and transformed into BCY123 yeast cells. A fermentor is used to incubate the BCY123 yeast cells in a large quantity. After breaking cells, the recombinant BoCesA proteins were purified by immobilized metal affinity chromatography and size-exclusion chromatography. This method resulted in 7.2mg total proteins from 120 g yeast cells. In the enzyme activity assay of BoCesAs, the long fiber-like products could be observed by a transmission electron microscope and confirmed as the product β-1,4-glucan by partially methylated alditol acetate-coupled gas chromatography-mass spectrometry analysis. Therefore, a procedure by a heterologous expression, purification strategy, and enzyme activity analysis for BoCesAs was established for further studies. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:16:33Z (GMT). No. of bitstreams: 1 ntu-108-F00b42038-1.pdf: 23599838 bytes, checksum: dc9cbb3b06c18865b00771b85301559b (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III 縮寫對照表 IV 目錄 VI 圖表目錄 IX 附錄目錄 X 附圖目錄 XI 第一章 緒論 1 1.1 生質能發展趨勢 1 1.2 植物細胞壁結構 2 1.3 纖維素與纖維素微纖維 3 1.4 植物纖維素合成酶 4 1.4.1 植物纖維素合成酶蛋白質序列保守區分析 4 1.4.2 植物纖維素合成酶蛋白質之後轉譯修飾 6 1.4.3 阿拉伯芥纖維素合成酶分子生物學研究 6 1.4.4 植物纖維素合成酶的演化分析 7 1.4.5 調控植物纖維素合成酶的轉錄因子 7 1.4.6 植物纖維素合成偵測方法 8 1.4.7 細菌纖維素合成催化機制 8 1.5 植物纖維素合成酶複合體 9 1.5.1 阿拉伯芥纖維素合成酶複合體之參與成員 9 1.5.2 參與植物纖維素合成酶複合體之非CesA蛋白 10 1.5.3 植物體內CesA成員的排列、聚體及比例分析 11 1.5.4 初級與次級細胞壁合成時纖維素合成酶表現量的轉換 12 1.5.5 其他參與在纖維素合成中的重要蛋白質 12 1.5.6 植物纖維素合成酶於植物細胞內之移動行為 13 1.6 本研究之研究緣起 14 1.7 本研究之研究目標 14 第二章 材料與方法 16 2.1 實驗材料 16 2.2 以原核生物大腸桿菌測試纖維素合成酶表現 16 2.2.1 大腸桿菌載體構築 16 2.2.2 大腸桿菌勝任細胞之製備 16 2.2.3 大腸桿菌質體DNA轉型作用 17 2.2.4 大腸桿菌小量表現 17 2.3 以真核生物酵母菌測試纖維素合成酶表現以及大量純化 17 2.3.1 酵母菌載體構築 17 2.3.2 酵母菌質體DNA轉型作用 18 2.3.3 酵母菌小量表現 18 2.3.4 酵母菌利用生物反應器大量表現MBP-BoCesAs重組蛋白 19 2.3.5 重組蛋白MBP-BoCesAs的大量純化 19 2.3.6 透過粒徑排阻層析純化多聚體BoCesAs 20 2.4 蛋白質分析實驗 21 2.4.1 十二烷基硫酸鈉聚丙烯醯胺凝膠電泳 21 2.4.2 西方墨點法 21 2.4.3 蛋白質定量 22 2.4.4 利用穿透式電子顯微鏡觀察BoCesAs蛋白質 22 2.5 BoCesA重組蛋白之酵素活性分析 22 2.5.1 BoCesA重組蛋白樣品內1,4-glucan鍵結鑑定與去除 22 2.5.2 BoCesA重組蛋白之酵素活性測定 23 2.5.3 泛甲基醣醇乙酸衍生化 (PMAA衍生化) 23 2.5.4 氣相層析質譜儀分析 24 2.5.5 利用穿透式電子顯微鏡觀察BoCesA合成之纖維素微纖維 25 第三章 結果 26 3.1 纖維素合成酶蛋白質於異源系統之表現 26 3.2 MBP-BoCesA重組蛋白純化 27 3.3 纖維素合成酶多聚體之分子量分析 27 3.4 纖維素合成酶於穿透式電子顯微鏡下之影像 28 3.5 BoCesAs蛋白質樣品內之1,4-glucan 28 3.6 纖維素合成酶活性分析 29 3.7 纖維素合成酶酵素反應後於穿透式電子顯微鏡下之影像 29 第四章 討論 31 4.1 纖維素合成酶蛋白質表現及純化 31 4.2 纖維素醣類鍵結的確立 32 4.3 纖維素合成酶蛋白質酵素活性分析 32 4.4 纖維素合成酶蛋白質多聚體形式及複合體結構 33 4.5 纖維素合成酶蛋白質結構 34 4.6 纖維素合成酶與其他相關蛋白質之交互作用 35 第五章 結論 36 第六章 參考文獻 37 第七章 圖表 47 第八章 附錄 70 第九章 附圖 95 | |
dc.language.iso | zh-TW | |
dc.title | 竹纖維素合成酶在酵母菌之異源表現、純化及功能分析 | zh_TW |
dc.title | Heterologous expression, purification and functional analysis of bamboo cellulose synthases from yeast | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 謝旭亮(Hsu-Liang Hsieh),鄭秋萍(Chiu-Ping Cheng),王雅筠(Ya-Yun Wang),王愛玉(Ai-Yu Wang) | |
dc.subject.keyword | 纖維素合成?,醣基轉移?,膜蛋白純化,纖維素微纖維,纖維素合成?複合體, | zh_TW |
dc.subject.keyword | CesA protein,Glycosyltransferase,Membrane protein purification,Cellulose microfibril,Cellulose synthase complex, | en |
dc.relation.page | 115 | |
dc.identifier.doi | 10.6342/NTU201903344 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-15 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 23.05 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。