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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 蔡詩偉(Shih-Wei Tsai) | |
dc.contributor.author | Tse Wei Chao | en |
dc.contributor.author | 趙澤瑋 | zh_TW |
dc.date.accessioned | 2021-06-17T08:13:45Z | - |
dc.date.available | 2024-08-27 | |
dc.date.copyright | 2019-08-27 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-14 | |
dc.identifier.citation | [1] 侯珮萱、廖怡清、蔡宜芳、楊千慧、劉芳銘、黃承澤…林薃紋(2018)。106 年度市售農產品殘留農藥監測。食品藥物研究年報,(9),125-139。
[2] 食品藥物管理署(2019)。蔬果農藥殘留量監測結果。取自:http://www.fda.gov.tw/TC/site.aspx?sid=2428 [3] 翁志弘(2016)。農藥市場發展現況及趨勢。政策研究指標資料庫。 [4] Aktar, W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in Related studies [5] 行政院農業委員會(2018)。什麼是農藥。取自:https://pesticide.baphiq.gov.tw/web/briefDetailView.aspx?sn=15 [6] 行政院農業委員會(2018)。我國農藥管理及其展望。取自:https://pesticide.baphiq.gov.tw/web/briefDetailView.aspx?sn=34 [7] 行政院衛生福利部食品藥物管理署(民國105年12月12日修正發布)。殘留農藥安全容許量。 [8] 吳仲凱(2008)。臺北地區兒童農藥暴露及其與注意力缺陷過動症之相關性。 國立陽明大學環境與職業衛生研究所碩士論文。 [9] 邵佾萱(2015)。產前有機磷農藥暴露與出生結果關係研究。國立陽明大學環境與職業衛生研究所碩士論文。 [10] Schilter, B., Renwick, A. G., & Huggett, A. C. (1996). Limits for pesticide residues in infant foods: a safety-based proposal. Regulatory toxicology and pharmacology, 24(2), 126-140. [11] Shelton, J. F., Geraghty, E. M., Tancredi, D. J., Delwiche, L. D., Schmidt, R. J., Ritz, B., ... & Hertz-Picciotto, I. (2014). Neurodevelopmental disorders and prenatal residential proximity to agricultural pesticides: the CHARGE study. Environmental health perspectives, 122(10), 1103. [12] Lu, C., Bravo, R., Caltabiano, L. M., Irish, R. M., Weerasekera, G., & Barr, D. B. (2005). The presence of dialkylphosphates in fresh fruit juices: implication for organophosphorus pesticide exposure and risk assessments. Journal of Toxicology and Environmental Health, Part A, 68(3), 209-227. [13] Sunaga, M., Yoshida, M., & Hara, I. (1989). Metabolism and urinary excretion of chlorpyrifos in rats. Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene), 43(6), 1124-1129. [14] Ellington, J. J., Stancil, F. E., Payne, W. D., & Trusty, C. D. (1988). Measurement of hydrolysis rate constants for evaluation of hazardous-waste land disposal. Volume 3. Data on 70 chemicals (No. PB-88-234042/XAB; EPA-600/3-88/028). Environmental Protection Agency, Athens, GA (USA). Environmental Research Lab. [15] Ellington, J. J., Stancil, F. E., Payne, W. D., & Trusty, C. (1987). Measurement of hydrolysis rate constants for evaluation of hazardous-waste land disposal. Volume 2. Data on 54 chemicals (No. PB-87-227344/XAB). Environmental Protection Agency, Athens, GA (USA). Environmental Research Lab. [16] Gallardo, E., Barroso, M., Margalho, C., Cruz, A., Vieira, D. N., & López-Rivadulla, M. (2006). Determination of parathion in biological fluids by means of direct solid-phase microextraction. Analytical and bioanalytical chemistry, 386(6), 1717-1726. [17] Beltrán, J., López, F. J., & Hernández, F. (2000). Solid-phase microextraction in pesticide residue analysis. Journal of chromatography A, 885(1-2), 389-404. [18] World Health Organization. (2010). Pesticide Residues in Food 2007: Toxicological Evaluations (Vol. 184). World Health Organization. [19] Kissel, J. C., Curl, C. L., Kedan, G., Lu, C., Griffith, W., Barr, D. B., ... & Fenske, R. A. (2005). Comparison of organophosphorus pesticide metabolite levels in single and multiple daily urine samples collected from preschool children in Washington State. Journal of Exposure Science and Environmental Epidemiology, 15(2), 164. [20] Pawliszyn, J. (1997). Solid phase microextraction: theory and practice. John Wiley & Sons. [21] Risticevic, S., Lord, H., Gorecki, T., Arthur, C. L., & Pawliszyn, J. (2010). Protocol for solid-phase microextraction method development. Nature protocols, 5(1), 122. [22] Pragst, F. (2007). Application of solid-phase microextraction in analytical toxicology. Analytical and Bioanalytical Chemistry, 388(7), 1393-1414. [23] Lacorte, S., Guiffard, I., Fraisse, D., & Barceló, D. (2000). Broad spectrum analysis of 109 priority compounds listed in the 76/464/CEE council directive using solid-phase extraction and GC/EI/MS. Analytical chemistry, 72(7), 1430-1440. [24] Baudry, J., Debrauwer, L., Durand, G., Limon, G., Delcambre, A., Vidal, R., ... & Lairon, D. (2018). Urinary pesticide concentrations in French adults with low and high organic food consumption: results from the general population-based NutriNet-Santé. Journal of exposure science & environmental epidemiology, 1. [25] Reyes, J. G. G., & Alegria, H. (2018). TOXIC EFFECTS OF EXPOSURE TO PESTICIDES IN FARM WORKERS IN NAVOLATO, SINALOA (MEXICO). Revista Internacional de Contaminación Ambiental, 34(3), 505-516. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73919 | - |
dc.description.abstract | 近年來由於層出不窮的食品安全事件,使得國人對於食品安全議題愈發重視。其中,因農藥被大量應用於農產品、家庭、娛樂場所中,農藥殘留一直是台灣一個相當重要的食品安全議題。農藥不但遍及於生活中,研究報告也指出台灣的農藥使用量高於美國、日本;且農藥也有潛藏的健康危害,故如何針對易感族群如兒童進行暴露、風險評估,以維護國人健康是一項重要的議題。先前在台灣已有許多這方面的研究,然而這些研究大多著重於農藥代謝物的偵測。由於許多不同種類的農藥可能會產生同樣的代謝產物,可能會導致農藥暴露量與農藥暴露種類估計上的困難;故本研究使用固相微萃取技術,搭配氣相層析質譜儀,針對兒童尿液中的農藥原型物進行偵測。本研究成功建立了一套同時測量34種農藥原型物的方法,並於180個兒童晨尿樣本中測得了4-Chloroaniline、Pentachlorobenzene、1-Naphthylamine、2-Naphthylamine、Thionazin和Chlorpyrifos等六種農藥殘留。殘留濃度在個體間差異甚大,濃度範圍從2.90 ng/mL到56.94 ng/mL之間。平均濃度最高之農藥為Thionazin(5.49 ng/mL),1-Naphthylamine次之(3.81 ng/mL)。Thionazin也是最常被偵測到的農藥,與2-Naphthylamine皆有20%左右的樣本中含有此兩種農藥。在與問卷調查結果比對後,發現農藥殘留量和飲食攝取量及環境息息相關。例如尿液中1-Naphthylamine的含量與蔬菜的食用量為正相關,且達統計上的顯著(P-value為0.04)。本研究也發現兒童尿液中的農藥平均濃度高於別國,例如墨西哥農民尿液中Thionazin濃度0.33 ng/mL,檢出率18%;但本研究發現兒童尿液中Thionazin濃度是5.49 ng/mL,檢出率則為22%。這顯示了農藥殘留管理在台灣仍是一個重要議題。 | zh_TW |
dc.description.abstract | Pesticide residues in foods have become a food safety issue in Taiwan due to the extensive use in agriculture and household have raised health concerns. For example, the noncompliance rate for certain kinds of vegetables and fruits was above 30 percent. Besides, most commonly used pesticides, such as organophosphates and pyrethroids, are neurotoxic. The associated exposures might affect children’s neurodevelopment. In order to prevent the unwanted consequences, it is critical to characterize the amount of exposure and the type of pesticides. In Taiwan, many studies focused on measuring the urinary metabolites of pesticide instead of parental compounds. However, organophosphate pesticides generate the same metabolites such as dialkylphosphate (DAP), which can lead to underestimate of the total amount of pesticides and makes it more difficult to execute an exposure assessment. Hence, this study developed an approach by using direct immersion solidphase microextraction (DI-SPME) coupled with gas chromatography-tandem mass spectrometers (GC-MS/MS) to measure 34 parent compounds at the same time to solve the problem. An ideal parameter were established for detecting pesticide parent compounds in urine and the detection limit was 0.5 ng/mL. Six pesticide residues were found in 180 first-morning-void urine samples, including 4-Chloroaniline, Pentachlorobenzene, 1-Naphthylamine, 2-Naphthylamine, Thionazin, and Chlorpyrifos. The concentration varied from 2.90 ng/mL to 56.94 ng/mL among different samples. Thionazin and 1-Naphthylamine had higher detection rate and mean concentration. Based on the analysis of questionnaire, we discovered that urinary concentration of 1-Naphthylamine was associated with vegetables consumption (P-value = 0.04); 4-Chloroaniline was associated with open burning waste (P-value = 4.9×10-11) and steel mill (P-value = 0.0003); Chlorpyrifos was associated with odor around the house (P-value = 0.001). This study also discovered that the amount of exposure of children in Taiwan was higher than the amount of adults in France and Mexico. This result demonstrates the fact that pesticide residues are still an issue in Taiwan. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:13:45Z (GMT). No. of bitstreams: 1 ntu-108-R06851012-1.pdf: 1950873 bytes, checksum: 535956136b3b1549159366dcccdad2f9 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 中文摘要 i
ABSTRACT ii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES viii Chapter 1 Introduction 1 1.1 Pesticide use trends 1 1.2 Pesticides categories 1 1.3 Pesticides monitoring 2 1.4 Analytical methods of pesticide residues 3 1.4.1 Analytes 3 1.4.2 Specimen 3 1.4.3 Analysis 4 1.5 Objectives 4 Chapter 2 Materials and Methods 6 2.1 Participants 6 2.2 Reagent and chemicals 6 2.3 Sample preparation 6 2.4 SPME procedure 7 2.5 Instrumentation 7 2.6 Quantification 8 2.7 Quality assurance and quality control 8 2.8 Questionnaire 8 2.9 Statistical analysis 9 Chapter 3 Results 10 3.1 Optimization of DI-SPME procedure 10 3.2 Analysis of pesticides with GC–MS/MS 10 3.3 Results of questionnaire 11 Chapter 4 Discussion 12 Chapter 5 Conclusion 13 REFERENCE 14 | |
dc.language.iso | en | |
dc.title | 以固相微萃取偵測台灣兒童尿液中農藥殘留 | zh_TW |
dc.title | Solid-Phase Microextraction Procedure to Determine Pesticide Residues in Urine | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林嘉明(Jia-Ming Lin),王文忻(Ven-Shing Wang),陳美蓮(Mei-Lien Chen) | |
dc.subject.keyword | 農藥殘留,農藥原型物,兒童,固相微萃取,暴露評估, | zh_TW |
dc.subject.keyword | pesticide,parent compound,children,SPME,exposure assessment, | en |
dc.relation.page | 40 | |
dc.identifier.doi | 10.6342/NTU201903747 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-15 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 食品安全與健康研究所 | zh_TW |
顯示於系所單位: | 食品安全與健康研究所 |
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