請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73832
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張雅君(Ya-Chun Chang) | |
dc.contributor.author | Cheng-Hsiang Liao | en |
dc.contributor.author | 廖證翔 | zh_TW |
dc.date.accessioned | 2021-06-17T08:11:22Z | - |
dc.date.available | 2024-08-27 | |
dc.date.copyright | 2019-08-27 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-15 | |
dc.identifier.citation | Aladjadjiyan A. 2010. Effect of microwave irradiation on seeds of lentils (Lens culinaris, Med.). Rom. J. Biophys. 20: 213-221.
Araújo SDS, Paparella S, Dondi D, Bentivoglio A, Carbonera D, Balestrazzi A. 2016. Physical methods for seed invigoration: advantages and challenges in seed technology. Front. Plant Sci. 7: 646. Banik S, Bandyopadhyay S, Ganguly S. 2003. Bioeffects of microwave–a brief review. Bioresour. Technol. 87: 155-159. Bhaskara Reddy MV, Raghavan GSV, Kushalappa AC, Paulitz TC. 1998. Effect of microwave treatment on quality of wheat seeds infected with Fusarium graminearum. J. Agric. Eng. Res. 71: 113-117. Bhat NA, Syeed N, Bhat KA, Mir SA. 2010. Pathogenicity and host range of Xanthomonas campestris pv. campestris-incitant of black rot of crucifers. J. Phyto. 2: 01-05. Cebrián G, Condón S, Mañas P. 2017. Physiology of the inactivation of vegetative bacteria by thermo treatments: mode of action, influence of environmental factors and inactivation kinetics. Foods 6: 107. Chao YC, Hsu ST, Tzeng KC. 2010. Bactericidal efficacy of chlorine dioxide against three seed-borne plant pathogenic bacteria and application of seed treatment for eradication of these bacteria. Plant Pathol. Bull. 19: 19-29. Chen CW. Control of cabbage black rot by antagonistic Bacillus spp. Bioagent-the potential application. 2014. Doctoral thesis, National Chung Hsing University of Plant Pathology, Taiwan. (in Chinese) Chen HC. A simple method to differentiate Xanthomonas campestris pv. campestris and X. campestris pv. raphani isolated from cruciferous seeds. Master thesis, Fu Jen Catholic University of Live Science, Taiwan. (in Chinese) Chen RR, Ding CY, Lin HS. 2017. Vegetable seeds research and develop trend and benchmarking enterprise patents layout analysis. Agric. Tech. Ind. Inform. Platform. (in Chinese) (http://atiip.atri.org.tw/Report/PubReportShow.aspx?rpt_guid=4532e629-9cb4-401d-b921-926d9a8b9c04) Chen YT. 2017. Integrated control of the cactus scale, Diaspis echinocacti (Bouché) (Hemiptera: Diaspididae) in pitaya. Master thesis, National Taiwan University of Master Program for Plant Medicine. (in Chinese) Chiang WY, Wu MH, Wu KL, Lin MH, Teng HH, Tsai YF, Ko CC, Yang EC, Jiang JA, Barnett LR, Chu KR. 2014. A microwave applicator for uniform irradiation by circularly polarized waves in an anechoic chamber. Rev. Sci. Instrum. 85: 084703. Chu KR. 2005. Microwave and microwave application. Sci. Dev. 395: 28-37. (in Chinese) Dalton J. 1802. Experimental essays on the constitution of mixed gases; on the force of steam or vapor from waters and other liquids in different temperatures, both in a Torricellian vacuum and in air; on evaporation and on the expansion of gasses by heat. Mem. Proc. Manchester. Lit. Phil. Soc. 5: 535-602. Forsberg G. Control of cereal seed-borne disease by hot humid air treatment, Doctoral thesis, Swedish University of Agricultural Sciences. Retrieve from https://pub.epsilon.slu.se/516/ Friesen AP, Coner RL, Robinson DE, Barton WR, Gillard CL. 2014. Effect of microwave radiation on dry bean seed infection with Xanthomonas axonopodis pv. phaseoli with and without the use of chemical seed treatment. Crop Prot. 65: 77-85. Govers SK, Gayan E, Aertsen A. 2017. Intracellular movement of protein aggregates reveals heterogeneous inactivation and resuscitation dynamics in stressed populations of Escherichia coli. Environ. Microbiol. 19: 511-523. Grondeau C, Samson R. 1994. A review of thermotherapy to free plant materials from pathogens, especially seeds from bacteria. Crit. Rev. Plant Sci. 13: 57-75. Hankin L, Sands DC. 1977. Microwave treatment of tobacco seed to eliminate bacteria on the seed surface. Phytopathology 67: 794-795. Huang TC. 1988. Recent progress of research on control crucifer black rot in Taiwan. Proce. Symp. Veg. Breeding: 29-43. (in Chinese) Huang TC, Lee HL. 1988. Hot acidified zinc sulfate as seed soaking agent for the control of crucifer black rot. Plant Prot. Bull. 30: 245-258. (in Chinese) Humaydan HS, Harman GE, Nedrow BL, DiNitto LV. 1980. Eradication of Xanthomonas campestris, the causal agent of black rot, from Brassica seeds with antibiotics and sodium hypochlorite. Phytopathology 70: 127-131. International Rules for Seed Testing Association. 2019. 7-019b: ver 1.2 Detection of Xanthomonas campestris pv. campestris on Brassica spp. disinfested/disinfected seed with grinding. In: ISTA (eds). Seed Health Testing Methods. Switzerland. Jiao S, Zhong Y, Deng Y. 2016. Hot air-assisted radio frequency heating effects on wheat and corn seeds: Quality change and fungi inhibition. J. Stored Prod. Res. 69: 265-271. Kastelein P, Krijger MC, van der Zouwn PS, van der Steen JM, van der Wolf JM, Fernandes Vieira J, Amaral Villela F. 2014. Transmission of Xanthomonas campestris pv. campestris in seed production crops of cauliflower. 2nd Int. Symp. Org. Greenh. Hortic. 1: 197-204. Knox OGG, McHugh MJ, Fountaine JM, Havis ND. 2013. Effects of microwave on fungal pathogens of wheat seed. Crop Prot. 50: 12-16. Kocks CG, Ruissen MA, Zadoks JC, Duijkers MG. 1998. Survival and extinction of Xanthomonas campestris pv. campestris in soil. Eur. J. Plant Pathol. 104: 911-923. Kothari V, Mishra T, Kushwah P. 2014. Mutagenic effect of microwave radiation on exopolysaccharide production in Xanthomonas campestris. Curr. Trends Biotechnol. Pharm. 8: 29-37. Kuan TL, Minsavage GV, Schaad NW. 1986. Aerial dispersal of Xanthomonas campestris pv. campestris from naturally infected Brassica campestris. Plant Dis. 70: 409-413. Lee YA, Sung AN, Liu TF, Lee YS. 2009. Combination of chromogenic differential medium and estA-specific PCR for isolation and detection of phytopathogenic Xanthomonas spp. Appl. Environ. Microbiol. 75: 6831-6838. Li XH, Yang HJ, Roy B, Park EY, Jiang LJ, Wang D, Miao YG. 2010. Enhanced cellulose production of the Trichoderma viride mutated by microwave and ultraviolet. Microbiol. Res. 165: 190-198. Lin CY. 1981. Research on black rot disease in Taiwan. Plant Prot. Bull. 23: 157-167. (in Chinese) Massomo SM, Mortensen CN, Mabagala RB, Newman MA, Hockenhull J. 2004. Biological Control of Black Rot (Xanthomonas campestris pv. campestris) of Cabbage in Tanzania with Bacillus strains. J. Phytopath. 152: 98-105. McClean VER, Spheppard RJ, Grant EH. 1981. A generalized model for the interaction of microwave radiation with bound water in biological material. J. Microw. Power 16: 1-7. Mckeen WE. 1981. Black rot of rutabaga in Ontario and its control. Can. J. Plant Pathol. 3: 244-246. Meenu G, Vikram A, Bharat N. 2013. Black rot-a devastating disease of crucifers: a review. Agricult. Rev. 34: 269-278. Napoles P, Amat Z, Ramirez P. 1991. The use of different treatments to control Xanthomonas campestris pv. campestris in cabbage seed. Protection de plantas 1: 33-41. Nguyen THP, Shamis Y, Croft RJ, Wood A, Mclntosh RL, Crowford RJ, Ivanova EP. 2015. 18 GHz electromagnetic field induces permeability of Gram-positive cocci . Sci. Rep. 5: 10980. Park WCM, Maxwell AWP, Frank VE, Primmer MP, Collins SA, Baird GL, Dupuy DE. 2017. Evalution of a novel thermo accelerant for augmentation of microwave energy during image-guided tumor ablation. Theranostics 7: 1026-1035. Roberts SJ, Amein T, Frosberg G, Kromphardt C, Koch E, Schmitt A, Werner S. 2006. Physical and Biological seed treatment for control of bacterial disease of carrots and brassicas caused by Xanthomonas spp. 11th Int. Conference Plant Pathogenic Bac., Edinburgh, 10-14 July 2006. Roberts SJ, Brough J, Hunter PJ. 2007. Modelling the spread of Xanthomonas campestris pv. campestris in module-raised brassica transplants. Plant Pathol. 56: 391-401. Roberts SJ, Hiltunen LH, Hunter PJ, Brough J. 1999. Transmission from seed to seedling and secondary spread of Xanthomonas campestris pv. Campestris in Brassica transplants: effects of dose and water regime. Eur. J. Plant Pathol. 105: 879-889. Schaad NW, Dianese JC. 1981. Cruciferous weeds as sources of inoculum of Xanthomonas campestris in black rot of crucifers. Phytopathology 71: 1215-1220. Schaad, NW, Sitterly WR, Humaydan H. 1980. Relationship of incidence of seedborne Xanthomonas campestris to black rot of crucifers. Plant Dis. 64: 91-92. Schmidt M, Zannini E, Arendt EK. 2018. Recent advances in physical post-harvest treatment for shelf-life extension of cereal crops. Foods 7: 45. Schultz T, Gabrielson RL, Olson S. 1986. Contol of Xanthomonas campestris pv. campestris in crucifer seed with slurry treatments of calcium hypochlorite. Plant Dis. 70: 1027-1030. Seaman WL, Wallen VR. 1967. Effect of exposure to radio-frequency electric fields on seed-borne microorganisms. Can. J. Plant Sci. 47: 39-49. Seed Treatment and Environment Committee of the International Seed Federation. 2007. Seed treatment: a tool for sustainable agriculture. ISF. Switzerland. Retrieve from https://croplife.org/wp-content/uploads/pdf_files/Seed-Treatment-A-Tool-for-Sustainable-Agriculture.pdf Shamis Y, Croft R, Taube A, Crowford RJ, Ivanova EP. 2012. Review of the specific effects of microwave radiation on bacteria cells. Appl. Microb. Biotechnol. 96: 319-325. Sharma KK, Singh US, Sharma P, Kumar A, Sharma L. 2015. Seeds treatments for sustainable agriculture-a review. J. Appl. Nat. Sci. 7: 521-539. Sharma SL. 1981. Control of black rot of cauliflower by hot water seed treatment and field sprays with streptocycline. Indian J. Mycol. Plant Pathol. 11: 17-20. Shiomi T. 1992. Black rot cabbage seeds and its disinfection under a hot-air treatment. JARQ. 26: 13-18. Singh D, Mathur SB. 2004. Seed Infection by Bacteria. pp 169-192. In: Singh D, Muthur SB (eds). Histopathology of seed-borne infections. CRC Press. America. Stephenson MMP, Kushalappa AC, Raghavan GSV. 1996. Effect of selected combinations of microwave treatment factors on inactivation of Ustilago nuda from barley seed. Seed Sci. Technol. 24: 557-570. Timila RD. 2001. Seed-borne infection of Xanthomonas campestris pv. campestris in cabbage and its control through seed treatment. Nepal J. Sci. Technol. 3: 5-8. Tsai YF, Barnett LR, Teng HH, Ko CC, Chu KR. 2017. A study of some inherent causes for non-uniform microwave heating. Phys. Plasmas 24: 103301. United States Department of Agriculture. 2011. 7CFR Part 201-Federal seed act regulations. In: USDA (eds). Federal Seed Act Regulations. USA. Retrieve from https://www.federalregister.gov/documents/2011/06/02/2011-13497/federal-seed-act-regulations. Ven der Wolf J. 2005. Infection of brassica seed with Xanthomonas campestris pv. campestris. Plant Res. Int. 363: 19-28. Ven der Wolf JM, Zouwen PS ven der, Heijden L ven der. 2013. Flower infection of Brassica oleracea with Xanthomonas campestris pv. campestris results in high levels of seed infection. Eur. J. Plant Pathol. 136: 103-111. Vicente JG, Holub EB. 2013. Xanthomonas cmpestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops. Mol. Plant Pathol. 14: 2-18. Weidauer A. 2017. Electron treatment of seed. 1st Int. Conf. Appl. Radiat. Sci. Technol. Retrieve from https://media.superevent.com/documents/20170426/b2cb3edbfea051c761989fbf2f565b84/a.-weidauer.pdf. Woo IS, Rhee IK, Park HD. 2000. Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure. Appl. Environ. Microbiol. 66: 2243-2247. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73832 | - |
dc.description.abstract | 十字花科蔬菜黑腐病是由黑腐病菌 (Xanthomonas campestris pv. campestris,簡稱XCC) 所引起的世界性嚴重病害,在臺灣地區影響甘藍菜的產量甚鉅,而防治上最有效的方法便是使用無帶菌的健康種子,減少初次接種源,以降低田間發病率。目前雖已經有許多種子消毒方法被開發出來,但多具有無法完全殺菌、降低種子發芽率或處理時間太長等缺點。因此本研究欲利用臺大物理系所開發之24 GHz高頻微波系統,建立一套快速且能有效消除十字花科蔬菜種子黑腐病菌之技術,期望能提供種子生產業者全新的種子處理選項。首先以300 W高頻微波照射 XCC 1-1菌株之1 ml懸浮液,結果顯示8.91秒便能將XCC 1-1完全消除,說明了以高頻微波進行種子消毒的可能性。於是將甘藍種子浸泡於XCC 1-1懸浮液,風乾1天後作為人工接菌種子,平均每粒帶菌種子約有6萬CFU (colony forming unit) 的菌量。當直接以人工接菌的乾種子進行高頻微波的照射55秒,種子平均溫度達90℃,雖可以降低種子帶菌量至52%,但發芽率亦下降為25%。為提升高頻微波的殺菌效果,嘗試將種子進行噴霧處理後再照射高頻微波,結果顯示以該方法將種子加熱至平均溫度94℃時,能降低種子帶菌量至24%,同時發芽率提高為58%,較直接以乾種子照射高頻微波效果更佳,顯示水分可能為提升殺菌效果的關鍵。為了確認水在殺菌過程中的角色,進一步比較乾燥 (dry)、噴霧 (misting) 及浸水 [包含種子浸水於倒立離心管 (water-soaking in inverted tube) 及種子浸水 (water-soaking) 於正立離心管兩種處理] 等四種種子處理,經200 W高頻微波照射後,種子溫度達80℃左右之細菌族群量及發芽率。結果顯示種子表面所覆蓋的水分越多,殺菌效果越好,在兩種種子浸水的處理中,皆能將XCC 1-1從種子上完全消除;而在發芽率試驗中,只有種子浸水 (water-soaking) 的處理在高頻微波照射後,發芽率仍可維持100%。於是種子浸水處理配合高頻微波成為最佳種子消毒方式。為進一步探討種子浸水 (water-soaking) 後照射高頻微波,種子達80℃高溫下仍然得以存活的關鍵,於是藉由調控高頻微波功率和照射時間,將甘藍種子加熱至不同溫度,以找出XCC 1-1死亡而種子得以存活的溫度區間。由實驗結果發現以600 W照射0.6~1秒的快速加熱下,甘藍種子最高存活溫度為91℃,同時種子與XCC 1-1的致死溫度差可達13℃以上;相對在20 W照射35秒的慢速加熱下,甘藍種子最高僅能承受63℃,種子與XCC 1-1的致死溫差亦降為11℃;顯示高功率高頻微波的快速加熱是種子得以在高溫存活的主要原因,並且加熱速度越快越有利於種子消毒。綜合以上結果,本研究成功開發出以甘藍種子浸水 (water-soaking) 方式配合照射600 W高頻微波1秒鐘,作為高頻微波的標準種子消毒方法。此外,本研究亦證實了高頻微波的照射不會影響甘藍種子發芽及植株的生長,且能有效將幼苗黑腐病之發病率由15%下降為0%,於是本研究成為世界首度成功利用微波進行種子消毒的案例。基於人工接菌種子的成功,本研究進一步嘗試利用該技術消除種子內部的XCC。為獲得自然感染的帶菌種子,將XCC 1-1懸浮液噴灑於油菜花上,待種子成熟後採收果莢,經表面消毒後,證實每粒種子內部帶菌量約為3,500~16,000 CFU。將經表面消毒之種內帶菌油菜種子以種子浸水 (water-soaking) 的方式照射高頻微波,可以消除99%的種內細菌量;在進一步嘗試後發現,事先將種子浸水10分鐘,再以種子浸水方式處理高頻微波,便能將種子內部XCC全數殺死,但種子也無法存活。此外,本研究欲瞭解高頻微波如何於有水環境下將XCC 1-1殺死,於是初步收集種子浸水後經高頻微波照射之XCC 1-1,其中一部分細菌經propidium iodide染色後以共軛焦顯微鏡觀察,發現XCC 1-1細胞均無螢光產生,表示細胞膜並未受到破壞;另一部分細菌塗佈於SYG-X培養基後,完全無法在培養基生長,代表XCC 1-1已死亡,且死亡原因應與細胞膜損害無關。同時在掃描式及穿透式電子顯微鏡的觀察中,發現XCC 1-1經高頻微波照射後會有細胞縮短及細胞質產生均勻分布的電子緻密小體,推論這些效應可能與XCC致死的機制有關,但仍有待後續實驗證實。 | zh_TW |
dc.description.abstract | Black rot of crucifers caused by Xanthomonas campestris pv. campestris (XCC) is one of the most important diseases in the world, and it affects the yield of cabbage especially in Taiwan. The most effective method for prevention and control of black rot disease is the use of healthy pathogen-free seeds to reduce the initial inoculum and thus to decrease the disease incidence in the field. Though many seed disinfection methodshavebeen developed, but most of these methods cannot eliminate pathogenic bacteria completely, affect seed germination, or require long processing time. Therefore, this study intends to use the 24 GHz high-frequency microwave system developed by Department of Physics at National Taiwan University to establish a technology which can quickly and effectively eliminate XCC from vegetable seeds. Therefore, a new seed disinfection option can be provided for seed producers. At first, 1 ml of XCC 1-1 strain suspension was irradiated with 300 W high frequency microwave, and the result showed that XCC 1-1 was completely eliminated in 8.91 seconds, indicating the possibility of seed sterilization by high frequency microwave. The cabbage seeds were immersed in XCC 1-1 suspension, air-dried for 1 day and used as the artificially inoculated seeds, and the average bacterial population per seed was about 60,000 CFU (colony forming unit). While these dry inoculated seeds were directly irradiated with high frequency microwave for 55 seconds and the average seed temperature reach 90℃, XCC 1-1 population decreased to 52%, and seed germination rate was also reduced to 25%. In order to improve the sterilization effect of high frequency microwave, the seeds were misting-treated and then irradiated with high frequency microwave.. The results indicated when the misting-treated seeds were heated to the average temperature of 94℃, bacterial population per seed was reduced to 24%, and germination rate increased to 58%. Because the bacteria elimination of misting-treated seeds were better than dry seeds after irradiation with microwave, indicating that water might be the key factor of improving sterilization effect. To confirm the role of water in sterilization process, bacterial population and germination rate of dry, misting, and water-treated (including water-soaking in inverted tube and in upright tube) seeds were analyzed after irradiated with 200 W high frequency microwave, at about 80℃. The results revealed that the more water seed surface was covered by, the better bactericidal effect it had. In both water-treated seed treatments, XCC 1-1 could be eliminated from seeds completely; but only seeds of water-soaking treatment (water-soaking in upright tube)could maintain 100% germination rate. Consequently, seed with water-soaking treatment and high frequency microwave irradiation is the best disinfection method. In order to further explore the reason seed after water-soaking microwave treatement can still survive at high temperature of 80℃, the cabbage seeds were heated to different temperatures by adjusting the high frequency microwave power and irradiation times, and then tried to find out the temperature range in which XCC 1-1 die but seeds survive. According to the experimental results, it was found that under rapid heating of 600 W microwave irradiation for 0.6-1 seconds, the highest survival temperature of cabbage seeds was 91℃, and the lethal temperature difference between seed and XCC 1-1 reached above 13℃. Whereas under slow heating of 20 W irradiation for 35 seconds, the highest survival temperature of cabbage seed was 63℃, and the lethal temperature between seed and XCC 1-1 was reduced to 11℃. It can be concluded that rapid heating of high power high frequency microwave is the main reason why seed can survive at high temperature, and fast heating rate, is benificial to seed disinfection. Based on the above results, this study successfully developed a standard seed disinfection protocol for high frequency microwave as following water-soaking seed were irradiated with 600 W high frequency microwave for 1 seconds. In addition, our study also demonstrated that high frequency microwave irradiation did not affect seed germination and plant growth, also it could effectively reduce the black rot disease incidence of seedlings from 15% to 0%. So this study became the world’s first successful case of seed disinfection using microwave. Because of the success on artificially inoculated seeds, we further attempted to use the same technology to eliminate XCC inside the seeds. To obtain naturally infected seeds, the XCC 1-1 suspension was sprayed on the rape flowers, and the seed pods were harvested after seed matured. These seeds were surface sterilized, and the internal bacterial population was determined as 3,500-16,000 CFU per seed. Surface-sterilized rape seeds treated with water-soaking microwave irradiation method could eliminate 99% of XCC inside the seed; in advance, it was found that seeds pre-soaking in water for 10 minutes then treated with water-soaking microwave irradiation method could eliminate XCC inside the seed completely, but seed cannot survive. Further more, to understand how XCC was eliminated by high-frequency microwave in a watery environment, XCC 1-1 was collected after water-soaking microwave irradiation treatment, and part of the bacteria were stained with propidium iodide and observed by confocal microscope. Beacuse there had no fluorescence in XCC 1-1 cells, it suggested that bacterial cell membranes were not damaged. Another part of irradiated XCC 1-1 was cultured on medium, but no colony was observed, indicating these bacteria were dead and cell membrane destruction seemed not the causal reason. According to the observations of scanning and transmission electron microscopy, it was found that XCC 1-1 cells became shortend and electron-dense objects appeared in cytoplasm after high frequency microwave irradiation. It is inferred that these effects may be related to the mechanism of XCC 1-1 death, but it still needs to be confirmed. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:11:22Z (GMT). No. of bitstreams: 1 ntu-108-R06645007-1.pdf: 4180870 bytes, checksum: c7c566b51c3757d1a1d552ca44e6e056 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 論文口試委員審定書 i
誌謝 ii 中文摘要 iv ABSTRACT vi 目錄 ix 表目錄 xiii 圖目錄 xiv 第 1 章 前言 1 1.1 十字花科黑腐病 1 1.2 XCC帶菌種子處理方法之介紹 3 1.3 高頻微波 6 1.4 微波種子處理 6 1.5 高頻微波研究之現況 8 1.6 微波對細菌的致死效應 8 1.7 研究動機 10 第 2 章 材料與方法 11 2.1 十字花科種子之種傳細菌檢測試驗 11 2.1.1 供試十字花科植物種子 11 2.1.2 菌株來源、培養及保存方法 11 2.1.3 以鑑別性培養基區分十字花科植物常見之種傳細菌 11 2.2 利用人工接菌種子開發殺菌技術 12 2.2.1 人工接菌種子之製備 12 2.2.2 種子表面帶菌量之檢測 12 2.2.3 種子發芽率之檢測 12 2.2.4 微波設備 13 2.2.5 人工接菌種子於不同時間後之帶菌量檢測 13 2.2.6 細菌懸浮液之微波殺菌試驗 13 2.2.7 甘藍種子之耐溫試驗 14 2.2.8 人工接菌乾種子之高頻微波殺菌及發芽率試驗 14 2.2.9 人工接菌噴霧處理種子之高頻微波殺菌及發芽率試驗 15 2.2.10 人工接菌種子之高頻微波殺菌條件試驗 15 2.2.11 甘藍種子及黑腐病菌之致死溫度試驗 16 2.2.12 人工接菌種子經高頻微波處理後之生長勢及發病率試驗 16 2.3 自然感染種子殺菌技術之開發 17 2.3.1 接種油菜植株以生產自然感染且種內帶菌種子 17 2.3.2 以二氧化氯溶液測試油菜種子之表面消毒效力 17 2.3.3 自然感染種內帶菌種子之製備 17 2.3.4 種子內部帶菌量之檢測 18 2.3.5 自然感染油菜種子之高頻微波殺菌試驗 18 2.3.6 預先種子浸水種子之種內高頻微波殺菌及發芽率試驗 18 2.4 高頻微波殺菌機制之分析試驗 19 2.4.1 以碘化丙啶(propidium iodide)染劑分析XCC 1-1 19 2.4.2 以掃描式電子顯微鏡觀察經微波處理之XCC 1-1 19 2.4.3 以穿透式電子顯微鏡觀察經微波處理之XCC 1-1 20 第 3 章 結果 22 3.1 十字花科種子之種傳細菌檢測試驗 22 3.1.1 以鑑別性培養基區分十字花科植物常見之種傳細菌 22 3.1.2 供試甘藍種子之XCC檢測結果 23 3.2 利用人工接菌種子開發殺菌技術 23 3.2.1 人工接菌種子之細菌殘存量變化 23 3.2.2 細菌懸浮液之微波殺菌試驗 24 3.2.3 甘藍種子之耐溫測試 24 3.2.4 人工接菌乾種子之高頻微波殺菌及發芽率試驗 25 3.2.5 人工接菌噴霧處理種子之高頻微波殺菌及發芽率試驗 25 3.2.6 人工接菌甘藍種子之高頻微波殺菌條件試驗 26 3.2.7 甘藍種子及十字花科黑腐病菌之致死溫度試驗 27 3.2.8 人工接菌甘藍種子經微波照射後之生長勢及發病率試驗 29 3.3 自然感染種子殺菌技術之開發 29 3.3.1 接種油菜植株以生產自然感染且種內帶菌種子 29 3.3.1 以二氧化氯溶液測試油菜種子之表面消毒效力試驗 30 3.3.2 高頻微波照射自然感染油菜種子之殺菌條件試驗 30 3.3.3 種子事先浸水後再照射高頻微波之殺菌及發芽率試驗 31 3.4 高頻微波殺菌機制之分析試驗 31 3.4.1 碘化丙啶(propidium iodide)染色之實驗結果 31 3.4.2 掃描式電子顯微鏡之觀察結果 32 3.4.3 穿透式電子顯微鏡之觀察結果 32 第 4 章 討論 34 4.1 十字花科種子之種傳細菌檢測試驗 34 4.2 利用人工接菌種子開發殺菌技術 34 4.3 自然感染種子殺菌技術之開發 39 4.4 高頻微波殺菌機制之分析試驗 40 4.5 總結與未來展望 42 參考文獻 44 附表 52 附圖 65 附錄 88 | |
dc.language.iso | zh-TW | |
dc.title | 以高頻微波消除甘藍種子之黑腐病菌之研究 | zh_TW |
dc.title | Study on eliminating Xanthomonas campestris pv.
campestris from cabbage seeds by high frequency microwave | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 柯俊成(Chiun-Cheng Ko) | |
dc.contributor.oralexamcommittee | 朱國瑞(Kwo-Ray Chu),李永安(Yung-An Lee) | |
dc.subject.keyword | 高頻微波,十字花科黑腐病菌,甘藍,種子消毒,致死溫度, | zh_TW |
dc.subject.keyword | high frequency microwave,Xanthomonas campestris pv. campestris,cabbage,seed disinfection,lethal temperature, | en |
dc.relation.page | 92 | |
dc.identifier.doi | 10.6342/NTU201903663 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
顯示於系所單位: | 植物醫學碩士學位學程 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 4.08 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。