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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 賴育英(Yu-Ying Lai) | |
| dc.contributor.author | Yi-Hsiang Huang | en |
| dc.contributor.author | 黃郁翔 | zh_TW |
| dc.date.accessioned | 2021-05-19T17:42:06Z | - |
| dc.date.available | 2024-03-19 | |
| dc.date.available | 2021-05-19T17:42:06Z | - |
| dc.date.copyright | 2019-03-19 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-03-07 | |
| dc.identifier.citation | 1. Lanyi, J. K., Bacteriorhodopsin. Annual Review of Physiology 2004, 66, 665-688.
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S., Two-Photon Excited Fluorescence of a Conjugated Polyelectrolyte and Its Application in Cell Imaging. ACS Applied Materials & Interfaces 2010, 2 (10), 2744-2748. 46. Geng, J.; Goh, C. C.; Tomczak, N.; Liu, J.; Liu, R.; Ma, L.; Ng, L. G.; Gurzadyan, G. G.; Liu, B., Micelle/Silica Co-protected Conjugated Polymer Nanoparticles for Two-Photon Excited Brain Vascular Imaging. Chemistry of Materials 2014, 26 (5), 1874-1880. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7361 | - |
| dc.description.abstract | 從嗜鹽古細菌的細胞膜上發現的細菌視紫紅質HmBRI之突變種HmBRI-D94N是一種由光驅動的氫離子幫浦。HmBRI-D94N內部的全反式retinal在吸收光能之後改變構形，進而使HmBRI-D94N依序放出和吸收一個氫離子。這個光敏感蛋白質可以在光照下瞬間酸化周遭環境，造成一個短時間的pH值變化。這在生醫領域裡可以做為感光裝置元件，藥物傳遞材料和偵測元件等應用。本實驗分成兩部分，第一部分為利用具有螢光性質的共軛高分子去改變HmBRI-D94N氫離子幫浦功能所需使用的驅動波長。HmBRI-D94N是吸收波長為550 nm附近的綠光而受驅動，結合會放出綠色螢光的共軛高分子，理論上可以在其他波長的光照下驅動HmBRI-D94N，延伸HmBRI-D94N的應用範圍。本實驗使用兩種高分子，poly(p-phenyleneethynylene)的離子性高分子PPESO3，以及poly[2,7-(9,9-dihexylfluorene)-alt-4,7-benzothiadiazole)] (PFBT)的奈米粒子。這兩種共軛高分子都具有藍光的吸收波段以及綠光的螢光波段，並且都有著吸收紅外光的雙光子吸收的性質。以高穿透跟低傷害的紅外光激發高分子，藉由螢光共振能量轉移(FRET)間接激發HmBRI-D94N為此實驗的目標。第二部分為氧化銦錫(ITO)導電玻璃的表面改質，目的是讓HmBR-D94N I能夠依附在導電玻璃之上。首先使用三乙酸基胺(NTA)跟鎳離子，跟HmBRI-D94N上的組氨酸標籤(histidine tag)形成錯合物。這方法雖然能成功固定HmBRI-D94N在ITO上，但不夠穩定，容易在水中解離。因此在形成錯合物之後，再用1-乙基-3-(3-二甲基氨基丙基)碳醯二亞胺 (3-(ethyliminomethyleneamino)-N,N-dimethyl-propan-1-amine，簡稱EDC)跟N-羥基琥珀醯亞胺(N-Hydroxysuccinimide，簡稱NHS)使HmBRI-D94N跟NTA之間形成更穩定的醯胺鍵結。結合HmBRI-D94N的氫離子釋放能力跟ITO因為環境酸鹼值改變電動勢的性質，可以作成偵測蛋白質交互反應的元件。 | zh_TW |
| dc.description.abstract | A new kind of bacteriorhodopsin HmBRI was found in Haloarcula marismortui. HmBRI-D94N is a light driven proton pump, which is functioned by a series of conformational changes of the retinal inside the protein in the presence of light illumination. It can pump out a proton and change the pH value of environment in a very short time. HmBRI-D94N can be engineered for application for biotechnology, including molecular memory devices, light-triggered drug delivery, and a protein sensor. The first part of this study focuses on changing the wavelength of stimulating light to drive HmBRI-D94N, by introducing water-soluble conjugated polymer. The proton-pump functionality of HmBRI-D94N is triggered by absorbing primarily green light. Theoretically speaking, a polymer which can convert other wavelengths into green fluorescence should be capable of activating HmBRI-D94N by Förster resonance energy transfer (FRET). PPESO3 (conjugated polyelectrolyte) and poly(fluorine-alt-benzothiadiazole) (PFBT, water-soluble nanoparticles) are examined for this purpose. The results indicate that the FRET can take place between PFBT and HmBRI-D94N. The second part is the surface modification of indium tin oxide (ITO) glass by HmBRI-D94N, aiming at stably fastening HmBRI-D94N onto the ITO glass. A combination of nitrilotriacetic acid and nickel ion was employed to absorb HmBRI-D94N by establishing the coordination between polyhistidine-tag of HmBRI-D94N and nickel. This modification can be achieved; however, the coordination is not stable enough. HmBRI-D94N can be easily removed from the ITO surface by water and in the presence of imidazole. (3-(Ethyliminomethyleneamino)-N,N-dimethyl-propan-1-amine (EDC) and N-hydroxysuccinimide (NHS) were then utilized to build covalent bond between HmBRI-D94N and NTA. The corresponding device performs good stability against water and even imidazole, validating that HmBRI-D94N can be stably fastened on the ITO glass by our approaches. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-19T17:42:06Z (GMT). No. of bitstreams: 1 ntu-108-R05549036-1.pdf: 3741416 bytes, checksum: c90b523a088258d2393817e29bad6e09 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 目錄 i
圖目錄 iii 表目錄 vi 中文摘要 vii Abstract viii 第一章：緒論 1 第一節：細菌視紫紅質HmBRI 1 1.1：HmBRI的基本介紹 1 1.2：HmBRI的運輸機制 3 1.3：研究動機 5 第二節：螢光共振能量轉移(FRET) 6 2.1：FRET的原理及相關應用 6 2.2：雙光子激發螢光的應用 9 第三節：氧化銦錫(ITO)的表面修飾 12 3.1：ITO修飾的基本介紹 12 3.2：Histidine-Tag與Ni2+的非共價性鍵結 15 3.3：EDC/NHS的交叉鍊接 17 第二章：結果與討論－蛋白質的能量傳遞 19 第一節：高分子的合成與性質 19 1.1：PPESO3的吸收光譜與螢光光譜 19 1.2：PFBT的吸收光譜與螢光光譜 23 第二節：PPESO3與HmBRI-D94N的能量轉移實驗 27 第三節：PFBT dots與HmBRI-D94N的能量轉移實驗 32 第三章：結果與討論－蛋白質的固定修飾 36 第一節：ITO表面修飾的實驗概述 36 第二節：Histidine tag非共價鍵結的光電化學測試 38 第三節：EDC-NHS共價鍵結的光電化學測試 41 第四章：總結 44 第五章：實驗細節 46 第一節：試藥 46 第二節：實驗儀器 46 2.1：核磁共振光譜儀(Nuclear Magnetic Resonance，NMR) 46 2.2：凝膠滲透層析儀(Gel Permeation Chromatograph，GPC) 46 2.3：紫外線與可見光光譜儀(UV-Vis Spectrophotometer) 47 2.4：螢光光譜儀(Fluorescence Spectrophotometer) 47 2.5：粒徑/介面電位分析儀(Particle Sizing and Zeta Potential) 47 2.6：光電化學實驗裝置(Photocurrent Device) 48 2.7：X光電子能譜分析儀(X-ray Photoelectron Spectroscopy) 48 第三節：合成 48 第四節：ITO表面修飾 51 參考資料 54 附錄：NMR光譜 58 附錄：X電子能譜分析圖 66 | |
| dc.language.iso | zh-TW | |
| dc.title | 視紫紅質用於ITO表面修飾及與共軛高分子間的能量轉移研究 | zh_TW |
| dc.title | Utilization of Rhodopsin in ITO Surface Modification and Study on the Energy Transfer between Rhodopsin and Conjugated Polymers | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊啟昇(Chii-Shen Yang),葉怡均(Yi-Chun Yeh),葉伊純(Yi-Cheun Yeh) | |
| dc.subject.keyword | 高分子,螢光,螢光共振能量轉移,表面修飾,蛋白質,視紫質, | zh_TW |
| dc.subject.keyword | polymer,fluorescence,FRET,surface modification,protein,rhodopsin, | en |
| dc.relation.page | 66 | |
| dc.identifier.doi | 10.6342/NTU201900290 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2019-03-08 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2024-03-19 | - |
| 顯示於系所單位： | 高分子科學與工程學研究所 | |
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