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DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Ming-Ye He | en |
dc.contributor.author | 何明曄 | zh_TW |
dc.date.accessioned | 2021-06-17T07:18:54Z | - |
dc.date.available | 2019-08-15 | |
dc.date.copyright | 2019-08-15 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-07-10 | |
dc.identifier.citation | [1] Cox,G.,et al.,3-dimensional imaging of collagen using second harmonic generation.J Struct Biol, 2003. 141(1): p. 53-62.
[2] Jester,J.V.,et al.,Expression of alpha-smooth muscle (alpha-SM) actin during corneal stromal wound healing. Invest Ophthalmol Vis Sci, 1995.36(5): p.809-19. [3] Oliva MS,Schottman T,Gulati M.Turning the tide of corneal blindness.Indian J Ophthalmol.2012;60:423–427 [4] Three-Dimensional Distribution of Transverse Collagen Fibers in the Anterior Human Corneal Stroma Moritz Winkler,Golroxan Shoa, Yilu Xie,Steven J.Petsche, Peter M.Pinsky, Tibor Juhasz,Donald J. Brown and James V.Jester [5] Chen,W.L.,et al.,Quantitative analysis of multiphoton excitation autofluorescence and second harmonic generation imaging for medical diagnosis.Comput Med Imaging Graph, 2012.36(7):p.519-26. [6] Trelstad, R.L. and A.J. Coulombre, Morphogenesis of the collagenous stroma in the chick cornea. J Cell Biol, 1971. 50(3): p. 840-58. [7] Morishige, N., et al., Second-harmonic imaging microscopy of normal human and keratoconus cornea. Invest Ophthalmol Vis Sci, 2007. 48(3): p. 1087-94. [8] Sun, T.L., et al., Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy. J Biomed Opt, 2010. 15(3): p. 036002. [9] Hsueh, C.M., et al., Structural characterization of edematous corneas by forward and backward second harmonic generation imaging. Biophys J, 2009. 97(4): p. 1198-205. [10] Lee, S.L., Y.F. Chen, and C.Y. Dong, Probing Multiscale Collagenous Tissue by Nonlinear Microscopy. Acs Biomaterials Science & Engineering, 2017. 3(11): p. 2825-2831. [11]光連雙月刊 2007/09 71期 非線性光學顯微術應用於生理及病理學的活體研究 [12] Thompson, Darcy Wentworth.“On growth and form.”On growth and form.(1942). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73131 | - |
dc.description.abstract | 在此論文中,主要目的是了解眼角膜在發育過程中,其膠原蛋白三維構造的變化。使用血清抗體陰性雞胚胎蛋,使其發育至9至19天的雞胚胎,而後取下其眼角膜(左眼以及右眼),各天數均使用三個雞胚胎,共六個眼角膜(左、右各三個)。再利用雙光子顯微鏡(second harmonic generation , SHG)的連續成像,並搭配快速傅立葉轉換(fast Fourier-transform , FFT)進行分析,藉此了解眼角膜的膠原蛋白在發育過程中其三維構造的變化,這對於發育生物學中的發育模式形成(Pattern Formation)是相當重要的問題。因此,我們亦對樣品進行染色,希望知道在發育過程中細胞、細胞核的變化以及細胞與膠原蛋白的關係,進而了解使眼角膜產生特定結構的機制,建立雞胚胎角膜發育的模型。 | zh_TW |
dc.description.abstract | The cornea is main refractive component of the eye.it has high optical clarity and acts as a barrier to the external environment. And such characteristics come from the structure of the cornea.Therefore, understanding the structure of the cornea is important work.
In this research project, we take 9-19 days chicken embryo’s cornea as sample. Next, we use second harmonic generation (SHG) microcopy to take images and fast Fourier-transform (FFT) to analyze images. We also analyze the data of the left and right eyes. The result show that, chicken cornea stroma rotate in a counterclockwise direction. Otherwise, the rotation rate decreases with depth. Upon reaching posterior stroma, the cornea’s collagen remains certain direction. We can confirm the rotation characteristics of collagen and help to understand the structure which contain numerous collagen. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:18:54Z (GMT). No. of bitstreams: 1 ntu-108-R05245011-1.pdf: 3974642 bytes, checksum: 24e334532966018845c288177f26d669 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書
誌謝1 中文摘要2 英文摘要3 目錄4 圖目錄6 表目錄8 Chapter1緒論9 Chapter 2實驗原理10 2.1雙光子以及二倍頻10 2.1.1雙光子與二倍頻原理說明12 2.1.2雙光子與二倍頻差異處13 2.2雞胚胎14 2.3眼角膜15 2.4染色16 2.5傅立葉轉換19 2.6發育模式形成20 Chapter3實驗儀器以及研究方法21 3.1雙光子顯微鏡21 3.2樣品製備24 3.3實驗操作25 Chapter4實驗結果與討論26 4.1以雙光子顯微鏡觀察角膜膠原蛋白26 4.2以雙光子顯微鏡搭配染色觀察角膜33 Chapter 5結論以及未來展望34 REFERENCE35 | |
dc.language.iso | zh-TW | |
dc.title | 雞胚胎的眼角模於發育過程中的結構變化 | zh_TW |
dc.title | Structural Change of Chicken Cornea during Development | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳永芳(Yang-Fang Chen),王立民(Li-Min Wang),張顏暉(Yuan-Huei Chang) | |
dc.subject.keyword | 眼角膜,雙光子顯微鏡,膠原蛋白,核酸染色,發育模式形成, | zh_TW |
dc.subject.keyword | cornea,two-photon microscopy,collagen,nucleic acid staining,pattern formation, | en |
dc.relation.page | 35 | |
dc.identifier.doi | 10.6342/NTU201901274 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-07-10 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 應用物理研究所 | zh_TW |
顯示於系所單位: | 應用物理研究所 |
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ntu-108-1.pdf 目前未授權公開取用 | 3.88 MB | Adobe PDF |
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