請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73082
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡坤憲(Kun-Hsien Tsai) | |
dc.contributor.author | Ya-Chih Cheng | en |
dc.contributor.author | 鄭雅之 | zh_TW |
dc.date.accessioned | 2021-06-17T07:16:43Z | - |
dc.date.available | 2023-12-25 | |
dc.date.copyright | 2021-02-23 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-12-25 | |
dc.identifier.citation | Azevedo, R. S. S., Sousa, J. R. D., Araujo, M. T. F., Filho, A. J. M., Alcantara, B. N. D., Araujo, F. M. C., … Vasconcelos, P. F. C. (2018). In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-017-17765-5 Barletta, A. B. F., Alves, L. R., Silva, M. C. L. N., Sim, S., Dimopoulos, G., Liechocki, S., … Sorgine, M. H. F. (2016). Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and dengue virus. Scientific Reports, 6(1). https://doi.org/10.1038/srep19928 Beasley, D. W., Li, L., Suderman, M. T., Barrett, A. D. (2002). Mouse neuroinvasive phenotype of West Nile virus strains varies depending upon virus genotype. Virology, 296(1), 17-23. https://doi.org/10.1006/viro.2002.1372 Bhatt, S., Gething, P., Brady, O., Messina J. P., Farlow A. W., Moyes C. L., … Hay S. I. (2013). The global distribution and burden of dengue. Nature, 496, 504-507. https://doi.org/10.1038/nature12060 Brasil, P., Pereira, J. P., Moreira, M. E., Nogueira, R. M. R., Damasceno, L., Wakimoto, M., … Nielsen-Saines, K. (2016). Zika virus infection in pregnant women in Rio de Janeiro. New England Journal of Medicine, 375(24), 2321-2334. https://doi.org/10.1056/nejmoa1602412 Brown, J. E., Mcbride, C. S., Johnson, P., Ritchie, S., Paupy, C., Bossin, H., … Powell, J. R. (2011). Worldwide patterns of genetic differentiation imply multiple ‘domestications’ of Aedes aegypti, a major vector of human diseases. Proceedings of the Royal Society B: Biological Sciences, 278(1717), 2446-2454. https://doi.org/10.1098/rspb.2010.2469 Brown, J. E., Evans, B. R., Zheng, W., Obas, V., Barrera-Martinez, L., Egizi, A., … Powell, J. R. (2013). Human impacts have shaped historical and recent evolution in Aedes aegypti, the dengue and yellow fever mosquito. Evolution, 68(2), 514-525. https://doi.org/10.1111/evo.12281 Chang, C., Ortiz, K., Ansari, A., Gershwin, M. E. (2016). The Zika outbreak of the 21st century. Journal of Autoimmunity, 68, 1-13. https://doi.org/10.1016/j.jaut.2016.02.006 Chernomordik, L. (1996). Non-bilayer lipids and biological fusion intermediates. Chemistry and Physics of Lipids, 81(2), 203-213. https://doi.org/10.1016/0009-3084(96)02583-2 Chotiwan, N., Andre, B. G., Sanchez-Vargas, I., Islam, M. N., Grabowski, J. M., Hopf-Jannasch, A., . . . Perera, R. (2018). Dynamic remodeling of lipids coincides with dengue virus replication in the midgut of Aedes aegypti mosquitoes. PLoS Pathogens, 14(2). https://doi.org/10.1371/journal.ppat.1006853 Cugola, F. R., Fernandes, I. R., Russo, F. B., Freitas, B. C., Dias, J. L., Guimarães, K. P., . . . Beltrão-Braga, P. C. (2016). The Brazilian Zika virus strain causes birth defects in experimental models. Nature, 534(7606), 267-271. https://doi.org/10.1038/nature18296 Cui, Z. C. (1989). Allowable limit of error in clinical chemistry quality control. Clinical Chemistry, 35(4), 630-631. https://doi.org/10.1093/clinchem/35.4.630 Cullis, P., Kruijff, B. D. (1979). Lipid polymorphism and the functional roles of lipids in biological membranes. Biochimica Et Biophysica Acta, 559(4), 399-420. https://doi.org/10.1016/0304-4157(79)90012-1 Davidsen, J., Mouritsen, O. G., Jørgensen, K. (2002). Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes. Biochimica Et Biophysica Acta, 1564(1), 256-262. https://doi.org/10.1016/s0005-2736(02)00461-3 Dennis, E. A. (2009). Lipidomics joins the omics evolution. Proceedings of the National Academy of Sciences, 106(7), 2089-2090. https://doi.org/10.1073/pnas.0812636106 Dick, G., Kitchen, S., Haddow, A. (1952). Zika virus (I). Isolations and serological specificity. Transactions of the Royal Society of Tropical Medicine and Hygiene, 46(5), 509-520. https://doi.org/10.1016/0035-9203(52)90042-4 Downer, R. G. H. (1985). Lipid metabolism. In G. A. Kerkut L. I. Gilbert (Eds.), Comprehensive insect physiology, biochemistry and pharmacology (pp. 77-114). Oxford: Pergamon. Epelboin, Y., Talaga, S., Epelboin, L., Dusfour, I. (2017). Zika virus: an updated review of competent or naturally infected mosquitoes. PLoS Neglected Tropical Diseases, 11(11). https://doi.org/10.1371/journal.pntd.0005933 Fast, P. G. (1966). A comparative study of the phospholipids and fatty acids of some insects. Lipids, 1(3), 209-215. https://doi.org/10.1007/bf02531874 Faye, O., Faye, O., Diallo, D., Diallo, M., Weidmann, M., Sall, A. (2013). Quantitative real-time PCR detection of Zika virus and evaluation with field-caught mosquitoes. Virology Journal, 10(1), 311. https://doi.org/10.1186/1743-422x-10-311 Gentile, C., Lima, J., Peixoto, A. (2005). Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae). Memórias Do Instituto Oswaldo Cruz, 100(6), 545-547. https://doi.org/10.1590/s0074-02762005000600008 Gliedman, J. B., Smith, J. F., Brown, D. T. (1975). Morphogenesis of Sindbis virus in cultured Aedes albopictus cells. Journal of Virology, 16(4), 913-926. https://doi.org/10.1128/jvi.16.4.913-926.1975 Goebel, S., Snyder, B., Sellati, T., Saeed, M., Ptak, R., Murray, M., … Kalkeri, R. (2016). A sensitive virus yield assay for evaluation of antivirals against Zika virus. Journal of Virological Methods, 238, 13-20. https://doi.org/10.1016/j.jviromet.2016.09.015 Hannun, Y. A., Obeid, L. M. (2011). Many ceramides. Journal of Biological Chemistry, 286(32), 27855-27862. https://doi.org/10.1074/jbc.r111.254359 Hematian, A., Sadeghifard, N., Mohebi, R., Taherikalani, M., Nasrolahi, A., Amraei, M., Ghafourian, S. (2016). Traditional and modern cell culture in virus diagnosis. Osong Public Health and Research Perspectives, 7(2), 77-82. https://doi.org/10.1016/j.phrp.2015.11.011 Hsu, F. F., Turk, J., Thukkani, A. K., Messner, M. C., Wildsmith, K. R., Ford, D. A. (2003). Characterization of alkylacyl, alk-1-enylacyl and lyso subclasses of glycerophosphocholine by tandem quadrupole mass spectrometry with electrospray ionization. Journal of Mass Spectrometry, 38(7), 752-763. https://doi.org/10.1002/jms.491 Janmey, P., Kinnunen, P. (2006). Biophysical properties of lipids and dynamic membranes. Trends in Cell Biology, 16(10), 538-546. https://doi.org/10.1016/j.tcb.2006.08.009 Kamal, M., Kenawy, M. A., Rady, M. H., Khaled, A. S., Samy, A. M. (2018). Mapping the global potential distributions of two arboviral vectors Aedes aegypti and Ae. albopictus under changing climate. PLoS One, 13(12). https://doi.org/10.1371/journal.pone.0210122 Kloet, F. M. V. D., Bobeldijk, I., Verheij, E. R., Jellema, R. H. (2009). Analytical error reduction using single point calibration for accurate and precise metabolomic phenotyping. Journal of Proteome Research, 8(11), 5132-5141. https://doi.org/10.1021/pr900499r Kraut, R. (2011). Roles of sphingolipids in Drosophila development and disease. Journal of Neurochemistry, 116(5), 764-778. https://doi.org/10.1111/j.1471-4159.2010.07022.x Kumar, S.S., Puttaraju, H.P. (2012). Improvised microinjection technique for mosquito vectors. Indian Journal of Medical Research, 136(6), 971-978. Leier, H. C., Weinstein, J. B., Kyle, J. E., Lee, J.-Y., Bramer, L. M., Stratton, K. G., … Tafesse, F. G. (2020). A global lipid map defines a network essential for Zika virus replication. Nature Communications, 11, 3652. https://doi.org/10.1038/s41467-020-17433-9 Lindenbach, B. D., Rice, C. M. (2003). Molecular biology of flaviviruses. Advances in Virus Research, 23-61. https://doi.org/10.1016/s0065-3527(03)59002-9 Malone, R. W., Homan, J., Callahan, M. V., Glasspool-Malone, J., Damodaran, L., Schneider, A. D. B., … Wilson, J. (2016). Zika virus: medical countermeasure development challenges. PLoS Neglected Tropical Diseases, 10(3). https://doi.org/10.1371/journal.pntd.0004530 Mckenzie, B. A., Wilson, A. E., Zohdy, S. (2019). Aedes albopictus is a competent vector of Zika virus: a meta-analysis. PLoS One, 14(5). https://doi.org/10.1371/journal.pone.0216794 Meer, G. V., Voelker, D. R., Feigenson, G. W. (2008). Membrane lipids: where they are and how they behave. Nature Reviews Molecular Cell Biology, 9(2), 112-124. https://doi.org/10.1038/nrm2330 Melo, C. F. O. R., Oliveira, D. N. D., Lima, E. D. O., Guerreiro, T. M., Esteves, C. Z., Beck, R. M., … Catharino, R. R. (2016). A Lipidomics approach in the characterization of Zika-infected mosquito cells: potential targets for breaking the transmission cycle. PLoS One, 11(10). https://doi.org/10.1371/journal.pone.0164377 Molloy, J. C., Sommer, U., Viant, M. R., Sinkins, S. P. (2016). Wolbachia modulates lipid metabolism in Aedes albopictus mosquito cells. Applied and Environmental Microbiology, 82(10), 3109-3120. https://doi:10.1128/aem.00275-16 Morrison, A. C., Gray, K., Getis, A., Astete, H., Sihuincha, M., Focks, D., … Scott, T. W. (2004). Temporal and geographic patterns of Aedes aegypti (Diptera: Culicidae) production in Iquitos, Peru. Journal of Medical Entomology, 41(6), 1123-1142. https://doi.org/10.1603/0022-2585-41.6.1123 Noorbakhsh, F., Abdolmohammadi, K., Fatahi, Y., Dalili, H., Rasoolinejad, M., Rezaei, F., … Nicknam, M. H. (2019). Zika virus infection, basic and clinical aspects: a review article. Iranian journal of public health, 48(1), 20-31. https://doi.org/10.18502/ijph.v48i1.779 Oliveira, W. K. D., França, G. V. A. D., Carmo, E. H., Duncan, B. B., Kuchenbecker, R. D. S., Schmidt, M. I. (2017). Infection-related microcephaly after the 2015 and 2016 Zika virus outbreaks in Brazil: a surveillance-based analysis. The Lancet, 390(10097), 861-870. https://doi.org/10.1016/s0140-6736(17)31368-5 Orchard, R. C., Wilen, C. B., Virgin, H. W. (2018). Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection. Nature Microbiology, 3(10), 1109-1114. https://doi.org/10.1038/s41564-018-0221-8 Past, P. G. (1971). Insect lipids. Progress in the Chemistry of Fats and Other Lipids, 11, 179-242. https://doi.org/10.1016/0079-6832(71)90006-1 Petelska, A. D., Figaszewski, Z. A. (2000). Effect of pH on the interfacial tension of lipid bilayer membrane. Biophysical Journal, 78(2), 812-817. https://doi.org/10.1016/s0006-3495(00)76638-0 Perera, R., Riley, C., Isaac, G., Hopf-Jannasch, A. S., Moore, R. J., Weitz, K. W., … Kuhn, R. J. (2012). Dengue virus infection perturbs lipid homeostasis in infected mosquito cells. PLoS Pathogens, 8(3). https://doi.org/10.1371/journal.ppat.1002584 Pluskal, T., Castillo, S., Villar-Briones, A., Orešič, M. (2010). MZmine 2: modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data. BMC Bioinformatics, 11(1). https://doi.org/10.1186/1471-2105-11-395 Rietveld, A., Neutz, S., Simons, K., Eaton, S. (1999). Association of sterol- and glycosylphosphatidylinositol-linked proteins with Drosophila raft lipid microdomains. Journal of Biological Chemistry, 274(17), 12049-12054. https://doi:10.1074/jbc.274.17.12049 Sanchez-Alvarez, M., Zhang, Q., Finger, F., Wakelam, M. J., Bakal, C. (2015). Cell cycle progression is an essential regulatory component of phospholipid metabolism and membrane homeostasis. Open Biology, 5(9), 150093. https://doi.org/10.1098/rsob.150093 Simonin, Y., Loustalot, F., Desmetz, C., Foulongne, V., Constant, O., Fournier-Wirth, C., . . . Salinas, S. (2016). Zika virus strains potentially display different infectious profiles in human neural cells. EBioMedicine, 12, 161-169. https://doi.org/10.1016/j.ebiom.2016.09.020 Smithburn, K. C. (1952). Neutralizing antibodies against certain recently isolated viruses in the sera of human beings residing in East Africa. Journal of immunology, 69(2), 223-234. Stiasny, K., Koessl, C., Heinz, F. X. (2003). Involvement of lipids in different steps of the Flavivirus fusion mechanism. Journal of Virology, 77(14), 7856-7862. https://doi.org/10.1128/jvi.77.14.7856-7862.2003 Stiban, J., Tidhar, R., Futerman, A. H. (2010). Ceramide synthases: roles in cell physiology and signaling. Advances in Experimental Medicine and Biology, 688, 60-71. https://doi.org/10.1007/978-1-4419-6741-1_4 Tang, C. H., Tsao, P. N., Chen, C. Y., Shiao, M. S., Wang, W. H., Lin, C. Y. (2011). Glycerophosphocholine molecular species profiling in the biological tissue using UPLC/MS/MS. Journal of Chromatography B, 879(22), 2095-2106. https://doi.org/10.1016/j.jchromb.2011.05.044 Tang, C. H., Tsao, P. N., Lin, C. Y., Fang, L. S., Lee, S. H., Wang, W. H. (2012). Phosphorylcholine-containing lipid molecular species profiling in biological tissue using a fast HPLC/QqQ-MS method. Analytical and Bioanalytical Chemistry, 404(10), 2949-2961. https://doi.org/10.1007/s00216-012-6414-8 Utermöhlen, O., Herz, J., Schramm, M., Krönke, M. (2008). Fusogenicity of membranes: the impact of acid sphingomyelinase on innate immune responses. Immunobiology, 213(3-4), 307-314. https://doi.org/10.1016/j.imbio.2007.10.016 Vazeille, M., Madec, Y., Mousson, L., Bellone, R., Barré-Cardi, H., . . . Failloux, A. B. (2019). Zika virus threshold determines transmission by European Aedes albopictus mosquitoes. Emerging microbes infections, 8(1), 1668-1678. https://doi.org/10.1080/22221751.2019.1689797 Vielle, N. J., Zumkehr, B., García-Nicolás, O., Blank, F., Stojanov, M., Musso, D., . . . Alves, M. P. (2018). Silent infection of human dendritic cells by African and Asian strains of Zika virus. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-23734-3 Wu, P., Yu, X., Wang, P. Cheng, G. (2019). Arbovirus lifecycle in mosquito: acquisition, propagation and transmission. Expert Reviews in Molecular Medicine, 21. https://doi.org/10.1017/erm.2018.6 Zhang, J., Zhang, Z., Chukkapalli, V., Nchoutmboube, J. A., Li, J., Randall, G., … Wang, X. (2016). Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites. Proceedings of the National Academy of Sciences, 113(8). https://doi.org/10.1073/pnas.1519730113 Zhang, Z., He, G., Filipowicz, N. A., Randall, G., Belov, G. A., Kopek, B. G., Wang, X. (2019). Host lipids in positive-strand RNA virus genome replication. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00286 Zhou, Y., Raphael, R. M. (2007). Solution pH alters mechanical and electrical properties of phosphatidylcholine membranes: relation between interfacial electrostatics, intramembrane potential, and bending elasticity. Biophysical Journal, 92(7), 2451-2462. https://doi.org/10.1529/biophysj.106.096362 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73082 | - |
dc.description.abstract | 白線斑蚊(Aedes albopictus)是全球重要的病媒之一,可傳播茲卡病毒感染症等蟲媒傳染病。這些病毒在感染細胞、細胞中複製和離開細胞時,都需要宿主提供脂質製膜狀構造。而脂質的結構、功能和脂質與病毒間的關係,可藉由脂質體學的方法來了解。先前的研究發現,部分RNA病毒與含磷酸膽鹼(phosphorylcholine)的脂質如磷脂醯膽鹼(phosphatidylcholine,PC)和鞘磷脂(sphingomyelin,SM)有關,並指出特定脂質的表現有助於病毒的複製和包裝。但由於多數的研究將焦點放在病毒與宿主間的關係,而非病毒與病媒間關係,使得相關研究較少。因此,本研究的目的是應用極致液相層析儀三段四極柱串聯式質譜儀,來了解受茲卡病毒亞洲型和非洲型感染的病媒細胞C6/36和經中胸注射的白線斑蚊雌蚊其頭胸部和腹部中的感染情況差異,與其含磷酸膽鹼脂質組成變化間的關係。 在蚊蟲細胞層級和蚊蟲個體層級的研究結果顯示在亞洲型和非洲型茲卡病毒的感染下,PCs和SMs相較於控制組有顯著表現量差異。首先,在兩者都觀察到PCs的表現量有顯著上升。另一方面,Lyso-PCs在受感染的細胞中顯著上升,但在蚊蟲腹部中卻呈下降趨勢。而SMs表現量變化則僅在細胞層級中觀察到,但在亞洲型與非洲型病毒的感染下有顯著差異。Lyso-PCs表現量的上升,與SMs表現量的下降,可能與非洲株病毒的感染速度和毒性有關。然而,在蚊蟲腹部中的僅發現同樣有上升變化的PCs,在Lyso-PCs和SMs中的發現與細胞層級有所不同,強調在蚊蟲個體和組織中進行實驗以反映脂質變化真實性的重要。因此,此研究描述了茲卡病毒亞洲型和非洲型在感染病媒細胞層級和個體層級的變化情形,並可增進對於茲卡病毒在病媒中感染、複製與脂質間關係更進一步的了解。 | zh_TW |
dc.description.abstract | Aedes albopictus is an important vector that can transmit vector-borne diseases such as Zika virus (ZIKV) disease. The infection of these arbovirus depends on hosts’ lipid-made membranes. Recent studies that applied lipidomic approaches demonstrated the perturbation of host phosphorylcholine-containing lipids like phosphatidylcholines (PCs) and sphingomyelins (SMs) at viral replication sites and packing vesicles that aid the formation of viral replication complexes (VRCs) and increase membrane fluidity. However, the understanding about ZIKV infection from the aspects of vectors are not well developed. This study aimed to provide information on the relationships between Asian strain and African strain of ZIKV infection and phosphorylcholine-containing lipid composition in the vector by applying UPLC-QqQ-MS/MS in order to compare the virulence and the expression of PCs and SMs in cell and individual levels. The results were acquired from the extracted lipid of ZIKV infected C6/36 cells and intrathoracically infected Aedes albopictus mosquitoes. And it showed that in both cell and individual levels, the lipid composition was significantly different between ZIKV Asian and African strains-infected ones and uninfected control. First, in infected C6/36 cells and mosquito bodies, PCs were observed to increase. On the other hand, the changing tendencies of Lyso-PCs were increasing in infected C6/36 cells but decreasing in mosquito bodies. As for SMs, the different expression levels were only observed in ZIKV Asian and African strain-infected C6/36 cells which could be related to the different virulence and the infection stage. The increasing levels of Lyso-PCs and the decreasing levels of SMs can explain the more rapid and virulent properties of ZIKV African strain. And the different expression of lipids between cell and individual levels indicated the complex environment in the whole mosquito, which emphasized on the importance of in vivo experiments. These novel findings can provide basic biological information for understanding the relationship between ZIKV infection, replication and lipid composition in mosquito vector. And provide insights of the lipid composition from the aspect of vectors. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:16:43Z (GMT). No. of bitstreams: 1 U0001-2512202004043300.pdf: 3009295 bytes, checksum: 9c0e1331a42f8b2bd0d52eb088a17292 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 口試委員會審定書 i 致謝 ii 中文摘要 iii Abstract iv Contents vi List of figures x List of tables xiii Chapter 1 Introduction 1 1.1 Zika virus disease 1 1.1.1 The pathogen: Zika virus 1 1.1.2 The vector: Aedes mosquitoes 1 1.1.3 The host: Homo sapiens 2 1.2 Phosphorylcholine-containing lipids 3 1.3 Relationship between ZIKV and phosphorylcholine-containing lipids 4 1.4 Lipidomics 4 1.5 Specific aims 5 Chapter 2 Materials and methods 7 2.1 Study design 7 2.2 Materials and methods 8 2.2.1 C6/36 cell culture 8 2.2.2 Virus 9 2.2.3 Plaque assay 9 2.2.4 ZIKV infction in C6/36 cells 10 2.2.5 RNA detection by RT-qPCR 10 2.2.6 Determining incubation period of ZIKV infection in C6/36 cells 11 2.2.7 Preparing ZIKV infected C6/36 cell samples 11 2.2.8 Preparing ZIKV-infected female Ae. albopictus mosquitoes 12 2.2.9 Lipid extraction 13 2.2.10 Phosphorylcholine-containing lipid profiling by UPLC-QqQ-MS/MS 14 2.2.11 Data processing 15 2.2.12 Analytical variation handling 15 2.2.13 Structural identification by UPLC-QqQ-MS/MS 16 2.2.14 Multivariate data analysis 16 2.2.15 Univariate data analysis 17 Chapter 3 Results and achievements 18 3.1 ZIKV infection in C6/36 cells 18 3.2 Phosphorylcholine-containing lipid composition of ZIKV infected and uninfected C6/36 cells 18 3.3 Multivariate analysis of effects of ZIKV on phosphorylcholine-containing lipid composition in infected and uninfected C6/36 cells 19 3.4 Univariate analysis of effects of ZIKV on phosphorylcholine-containing lipid composition in infected and uninfected C6/36 cells 21 3.5 Compring the phophorylcholine-containing lipid composition between ZIKV Asian and African strain-infected C6/36 cells 23 3.6 ZIKV infection in Ae. albopictus 24 3.7 Phosphorylcholine-containing lipid composition of ZIKV infected and uninfected Ae. albopictus 25 3.8 Multivariate analysis of effects of ZIKV on phosphorylcholine-containing lipid composition in infected and uninfected Ae. albopictus 25 3.9 Univariate analysis of effects of ZIKV on phosphorylcholine-containing lipid composition in infected and uninfected Ae. albopictus 26 3.10 Compring the phophorylcholine-containing lipid composition between ZIKV Asian and African strain-infected Ae. albopictus 28 Chapter 4 Discussion 29 4.1 Comparing the expression of ZIKV infected C6/36 cells between Asian and African strains 29 4.2 Comparing between control and infected group in ZIKV Asian strain-infected C6/36 cells 31 4.3 Perturbation of PCs in ZIKV Asian and African strain-infected C6/36 cells 31 4.4 Increasing levels of Lyso-PCs in ZIKV Asian and African strain-infected C6/36 cells 32 4.5 Increasing levels of specific PCs in ZIKV Asian and African strain-infected C6/36 cells 33 4.6 Increasing levels of SMs in ZIKV Asian strain-infected C6/36 cells 33 4.7 Decreasing levels of SMs in ZIKV African strain-infected C6/36 cells 34 4.8 Comparing the different expression of SMs between ZIKV Asian and African strain-infected C6/36 cells 35 4.9 Comparing the amount of ZIKV between Asian and African strains in infected Ae. albopictus 36 4.10 Perturbation of PCs in ZIKV Asian and African strain-infected Ae. albopictus 36 4.11 Decreasing levels of Lyso-PCs in ZIKV Asian and African strain-infected Ae. albopictus 37 4.12 Increasing levels of specific PCs in ZIKV Asian and African strain-infected Ae. albopictus 37 4.13 Comparing the different expression of phosphorylcholine-containing lipids between ZIKV Asian and African strain-infected C6/36 cells and Ae. albopictus 38 4.14 Limitation and future works 40 Chapter 5 Conclusion 42 References 43 Appendix 86 | |
dc.language.iso | en | |
dc.title | 應用三段四極柱串聯式質譜儀分析受茲卡病毒感染之白線斑蚊其含磷酸膽鹼脂質組成研究 | zh_TW |
dc.title | Using UPLC-QqQ-MS/MS based lipidomic approach to analyze phosphorylcholine-containing lipid compositions in Zika virus-infected Aedes albopictus | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林靖愉(Ching-Yu Lin) | |
dc.contributor.oralexamcommittee | 楊景程(Chin-Cheng Yang),范怡琴(Yi-Chin Fan),廖曉偉(Hsiao-Wei Liao) | |
dc.subject.keyword | 脂質體學,茲卡病毒,白線斑蚊,磷脂醯膽鹼,鞘磷脂, | zh_TW |
dc.subject.keyword | Lipidomics,Zika virus,Ae. albopictus,Phosphatidylcholine,Sphingomyelin, | en |
dc.relation.page | 86 | |
dc.identifier.doi | 10.6342/NTU202004460 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-12-29 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 環境與職業健康科學研究所 | zh_TW |
顯示於系所單位: | 環境與職業健康科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-2512202004043300.pdf 目前未授權公開取用 | 2.94 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。