請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72809
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林頌然(Sung-Jan Lin) | |
dc.contributor.author | Hung-Jui Chang | en |
dc.contributor.author | 張紘睿 | zh_TW |
dc.date.accessioned | 2021-06-17T07:06:49Z | - |
dc.date.available | 2023-08-19 | |
dc.date.copyright | 2019-08-19 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-07-24 | |
dc.identifier.citation | Brownell, I., E. Guevara, C. B. Bai, C. A. Loomis and A. L. Joyner (2011). 'Nerve-derived sonic hedgehog defines a niche for hair follicle stem cells capable of becoming epidermal stem cells.' Cell stem cell 8(5): 552-565.
Daley, W. P., S. B. Peters and M. Larsen (2008). 'Extracellular matrix dynamics in development and regenerative medicine.' J Cell Sci 121(Pt 3): 255-264. Fan, S. M.-Y., C.-F. Tsai, C.-M. Yen, M.-H. Lin, W.-H. Wang, C.-C. Chan, C.-L. Chen, K. K. L. Phua, S.-H. Pan, M. V. Plikus, S.-L. Yu, Y.-J. Chen and S.-J. Lin (2018). 'Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.' Biomaterials 167: 121-131. Festa, E., J. Fretz, R. Berry, B. Schmidt, M. Rodeheffer, M. Horowitz and V. Horsley (2011). 'Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling.' Cell 146(5): 761-771. Fleger-Weckmann, A., Y. Ustun, J. Kloepper, R. Paus, W. Bloch, Z. L. Chen, J. Wegner, L. Sorokin, L. Langbein, B. Eckes, P. Zigrino, T. Krieg and R. Nischt (2016). 'Deletion of the epidermis derived laminin gamma1 chain leads to defects in the regulation of late hair morphogenesis.' Matrix Biol 56: 42-56. Fujiwara, H., M. Ferreira, G. Donati, D. K. Marciano, J. M. Linton, Y. Sato, A. Hartner, K. Sekiguchi, L. F. Reichardt and F. M. Watt (2011). 'The basement membrane of hair follicle stem cells is a muscle cell niche.' Cell 144(4): 577-589. Gao, J., M. C. DeRouen, C.-H. Chen, M. Nguyen, N. T. Nguyen, H. Ido, K. Harada, K. Sekiguchi, B. A. Morgan, J. H. Miner, A. E. Oro and M. P. Marinkovich (2008). 'Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis.' Genes & development 22(15): 2111-2124. Gilles, C., J. A. Bassuk, H. Pulyaeva, E. H. Sage, J. M. Foidart and E. W. Thompson (1998). 'SPARC/osteonectin induces matrix metalloproteinase 2 activation in human breast cancer cell lines.' Cancer Res 58(23): 5529-5536. Gilmore, A. P. (2005). 'Anoikis.' Cell Death & Differentiation 12(2): 1473-1477. Hohenester, E. and P. D. Yurchenco (2013). 'Laminins in basement membrane assembly.' Cell adhesion & migration 7(1): 56-63. Huang, W. Y., S. F. Lai, H. Y. Chiu, M. Chang, M. V. Plikus, C. C. Chan, Y. T. Chen, P. N. Tsao, T. L. Yang, H. S. Lee, P. Chi and S. J. Lin (2017). 'Mobilizing Transit-Amplifying Cell-Derived Ectopic Progenitors Prevents Hair Loss from Chemotherapy or Radiation Therapy.' Cancer Res 77(22): 6083-6096. Imanishi, H., D. Tsuruta, C. Tateishi, K. Sugawara, R. Paus, T. Tsuji, M. Ishii, K. Ikeda, H. Kunimoto, K. Nakajima, J. C. Jones and H. Kobayashi (2010). 'Laminin-511, inducer of hair growth, is down-regulated and its suppressor in hair growth, laminin-332 up-regulated in chemotherapy-induced alopecia.' J Dermatol Sci 58(1): 43-54. Jahoda, C. A. B., K. A. Horne and R. F. Oliver (1984). 'Induction of hair growth by implantation of cultured dermal papilla cells.' Nature 311(5986): 560-562. Lee, Y. M., M. H. Bae, O. H. Lee, E. J. Moon, C. K. Moon, W. H. Kim and K. W. Kim (2004). 'Synergistic induction of in vivo angiogenesis by the combination of insulin-like growth factor-II and epidermal growth factor.' Oncol Rep 12(4): 843-848. Li, J., J. Tzu, Y. Chen, Y.-P. Zhang, N. T. Nguyen, J. Gao, M. Bradley, D. R. Keene, A. E. Oro, J. H. Miner and M. P. Marinkovich (2003). 'Laminin-10 is crucial for hair morphogenesis.' The EMBO journal 22(10): 2400-2410. Lu, P., K. Takai, V. M. Weaver and Z. Werb (2011). 'Extracellular matrix degradation and remodeling in development and disease.' Cold Spring Harb Perspect Biol 3(12). Miller, M. J., S. H. Wei, M. D. Cahalan and I. Parker (2003). 'Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy.' 100(5): 2604-2609. Miner, J. H., R. M. Lewis and J. R. Sanes (1995). 'Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues.' J Biol Chem 270(48): 28523-28526. Muller-Rover, S., B. Handjiski, C. van der Veen, S. Eichmuller, K. Foitzik, I. A. McKay, K. S. Stenn and R. Paus (2001). 'A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages.' J Invest Dermatol 117(1): 3-15. Myung, P. and M. Ito (2012). 'Dissecting the bulge in hair regeneration.' The Journal of clinical investigation 122(2): 448-454. Nikolova, G., N. Jabs, I. Konstantinova, A. Domogatskaya, K. Tryggvason, L. Sorokin, R. Fassler, G. Gu, H. P. Gerber, N. Ferrara, D. A. Melton and E. Lammert (2006). 'The vascular basement membrane: a niche for insulin gene expression and Beta cell proliferation.' Dev Cell 10(3): 397-405. Nishimura, E. K., S. A. Jordan, H. Oshima, H. Yoshida, M. Osawa, M. Moriyama, I. J. Jackson, Y. Barrandon, Y. Miyachi and S.-I. Nishikawa (2002). 'Dominant role of the niche in melanocyte stem-cell fate determination.' Nature 416(6883): 854-860. Ozbek, S., P. G. Balasubramanian, R. Chiquet-Ehrismann, R. P. Tucker and J. C. Adams (2010). 'The evolution of extracellular matrix.' Mol Biol Cell 21(24): 4300-4305. Ozdemir, E., Y. Kakehi, H. Okuno and O. Yoshida (1999). 'Role of matrix metalloproteinase-9 in the basement membrane destruction of superficial urothelial carcinomas.' J Urol 161(4): 1359-1363. Pöschl, E., U. Schlötzer-Schrehardt, B. Brachvogel, K. Saito, Y. Ninomiya and U. Mayer (2004). 'Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development.' Development 131(7): 1619. Paquette, B., C. Baptiste, H. Therriault, G. Arguin, B. Plouffe and R. Lemay (2007). 'In vitro irradiation of basement membrane enhances the invasiveness of breast cancer cells.' British journal of cancer 97(11): 1505-1512. Park, S., D. G. Gonzalez, B. Guirao, J. D. Boucher, K. Cockburn, E. D. Marsh, K. R. Mesa, S. Brown, P. Rompolas, A. M. Haberman, Y. Bellaïche and V. Greco (2017). 'Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice.' Nature cell biology 19(2): 155-163. Paus, R., B. Handjiski, S. Eichmuller and B. M. Czarnetzki (1994). 'Chemotherapy-induced alopecia in mice. Induction by cyclophosphamide, inhibition by cyclosporine A, and modulation by dexamethasone.' Am J Pathol 144(4): 719-734. Plikus, M. V., J. A. Mayer, D. de la Cruz, R. E. Baker, P. K. Maini, R. Maxson and C.-M. Chuong (2008). 'Cyclic dermal BMP signalling regulates stem cell activation during hair regeneration.' Nature 451: 340. Pozzi, A., P. D. Yurchenco and R. V. Iozzo (2017). 'The nature and biology of basement membranes.' Matrix biology : journal of the International Society for Matrix Biology 57-58: 1-11. Ramos-Lewis, W. and A. Page-McCaw (2019). 'Basement membrane mechanics shape development: Lessons from the fly.' Matrix Biol 75-76: 72-81. Rendl, M., L. Lewis and E. Fuchs (2005). 'Molecular dissection of mesenchymal-epithelial interactions in the hair follicle.' PLoS Biol 3(11): e331. Rompolas, P., E. R. Deschene, G. Zito, D. G. Gonzalez, I. Saotome, A. M. Haberman and V. Greco (2012). 'Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration.' Nature 487(7408): 496-499. Schneider, C. A., W. S. Rasband and K. W. Eliceiri (2012). 'NIH Image to ImageJ: 25 years of image analysis.' Nat Methods 9(7): 671-675. Timpl, R. (1996). 'Macromolecular organization of basement membranes.' Curr Opin Cell Biol 8(5): 618-624. Timpl, R. and J. C. Brown (1996). 'Supramolecular assembly of basement membranes.' Bioessays 18(2): 123-132. Yew, E., C. Rowlands and P. T. C. So (2014). 'Application of Multiphoton Microscopy in Dermatological Studies: a Mini-Review.' Journal of innovative optical health sciences 7(5): 1330010-1330010. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72809 | - |
dc.description.abstract | 毛囊是哺乳類動物皮膚的附屬物,具有定期再生用以保溫的能力。在體內平衡期間,外部微環境與來自於上皮細胞和間充質細胞的信號會調控毛囊生長週期。此外,游離輻射會導致毛囊進入萎縮性週期,其中包括萎縮性生長期和萎縮性衰退期甚至於導致掉髮。然而目前依然不清楚游離輻射導致地在毛囊再生過程中毛囊細胞的行為表現。在本實驗當中,我們通過活體影像和組織學分析闡明了輻射損傷後毛囊再生的機制。我們證明在受到2戈雷和5.5戈雷輻射照射後基底膜會失去其完整性。而且我們還追蹤了同一根受到游離輻射照射後的毛囊並發現它會進入萎縮性生長期。此外,我們通過縮時攝影活體影像觀察再生過程中上皮細胞及其後代的行為。在早期階段,凋亡細胞出現在毛囊基質當中,而外根鞘細胞停留在最初的位置。在受到5.5戈雷游離輻射照射48小時後,外根鞘細胞開始向下遷移並且試圖脫離基底膜。在照射後72到84小時,由於大量的細胞凋亡導致毛囊球逐漸消失。與此同時,上皮細胞也出現了顯著性的變化。透過我們的實驗,我們認為外根鞘細胞的搖擺向下運動和脫離基底膜都是由於基底膜的不完整而造成的,這為探索毛囊再生的機制提供了一個新的思路。 | zh_TW |
dc.description.abstract | Hair follicles are appendages of the mammalian skin with the capacity to regularly regenerate for temperature protection. During homeostasis, external microenvironment and intrinsic signals from epithelial cells and mesenchymal cells regulate hair cycle. Besides, radiation induces hair follicle dystrophies, including dystrophic catagen and dystrophic anagen, and even hair loss. However, the cellular behaviors in hair follicle regeneration from radiation remain unclear. In this study, we elucidate the mechanisms of hair follicle regeneration post-radiation injury using intravital imaging and histological analysis. We demonstrated that basement membrane loses its integrity after 2Gy and 5.5Gy radiation. After radiation, we also traced single hair follicle and found that the hair follicle entry dystrophic anagen. Also, we monitored the behavior of epithelial cells and their progeny during regeneration by long-term live imaging. In the early phase, apoptotic cells appeared in the matrix of the hair follicle and the outer root sheath cells stayed at the original position. After 48 hours after 5.5Gy radiation, the ORS cells began to migrate downward and attempted to escape from the basement membrane. Hair bulb gradually disappeared at 72-82 hours due to massive apoptosis. Meanwhile, the epithelial cell morphology dramatically changes. Our result suggesting that the swinging downward movement of ORS cells and the detachment of basement membrane are all due to the incompleteness of basement membrane, which brings a new thought to explore the mechanism of HF regeneration. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:06:49Z (GMT). No. of bitstreams: 1 ntu-108-R06548016-1.pdf: 1804111 bytes, checksum: a851b0298cc59f91114837579e73c924 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書..........................................#
中文摘要..................................................i ABSTRACT................................................ii CONTENTS...............................................iii LIST OF FIGURES..........................................v LIST OF TABLES..........................................vi Introduction.............................................1 The structure of hair follicle...........................1 The cycle of hair follicle...............................2 Niche (microenvironment) of hair follicle................2 Hair follicle dystrophic model by ionizing radiation.....3 Intravital imaging.......................................4 Materials and Methods....................................6 Mice.....................................................6 Synchronization of the hair follicle cycle...............6 Radiation................................................7 Immunofluorescence staining..............................7 Intravital imaging.......................................7 Animal prepares for live imaging.........................8 Imaging processing.......................................9 Results.................................................10 Hair follicle basement membrane integrity changed from anagen to catagen.......................................10 Hair follicle basement membrane was damaged after irradiation injury......................................12 Irradiation-induced HF dystrophic change can be observed through two-photon microscopy...........................13 ORS cells did not migrate significantly in dystrophic anagen during 12 to 24 hours after 5.5 Gy IR injury.....16 ORS cells migrated downward and also attempted to migrated inward to IRS during 48 to 60 hours after irradiation injury..................................................18 Hair bulb shrank gradually and accompanied by massive apoptotic fragment during 72 to 82 hours after 5.5 Gy IR injury..................................................20 Discussion..............................................22 References..............................................25 | |
dc.language.iso | en | |
dc.title | 探討基底膜在毛囊再生過程中所扮演的角色 | zh_TW |
dc.title | The Role of Basement Membrane in Hair Follicle Regeneration | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊宗霖,冀宏源 | |
dc.subject.keyword | 毛囊,游離輻射傷害,組織再生,細胞凋亡,基底膜,細胞遷移, | zh_TW |
dc.subject.keyword | Hair follicle,Ionizing radiation injury,Regeneration,Apoptosis,Basement membrane,Migration, | en |
dc.relation.page | 29 | |
dc.identifier.doi | 10.6342/NTU201901922 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-07-25 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 1.76 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。