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Research on the Dental Implant Surface Properties and Performance Using Atomic Layer Deposition
atomic layer deposition,zirconium oxide,aluminum oxide,alkaline phosphatase,osteocalcin,osseointegration,atomic force microscope,
|Publication Year :||2019|
Dental implant has become popular treatment option in modern dentistry. Osseointegration is one of the key factors that influence the treatment outcome of implantation. Moreover, the surface treatment of implant plays an important role in osseointegration.
The research modified the implant surface by using atomic layer deposition for coating ZrO2 and Al2O3 with different thickness (0nm, 25nm, 50nm, 100nm) on commercially pure titanium and Ti6Al4V discs respectively. Surface roughness and 3D image was measured and calculated by atomic force microscope. Surface morphology was investigated by scanning electron microscope. The cell viability test was evaluated using alamar blue assay and cell morphology was detected by immunofluorescence assay. Alkaline phosphatase assay and osteocalcin were used as early and late biochemical markers for evaluating osteoblast bone mineralization respectively.
The results showed the surface roughness of ZrO2 and Al2O3 coatings were both in nanoscale topography. Furthermore, the thicker coatings resulted in higher surface roughness. Scanning electron microscope analysis revealed characteristic differences between different coating materials. The crystals on the ZrO2 coatings were more evident than Al2O3 coatings.
ZrO2 coatings demonstrated better results in alamar blue assay and alkaline phosphate activity than non-coating samples whereas there were no significant differences between Al2O3 coatings and non-coating samples. HEPM cell morphology was also influenced by the surface treatment. The morphology on ZrO2 coatings presented more filament structures whereas Al2O3 coatings seems more flattened. As for the osteocalcin assay, the result of each group was lower than control group due to the experiment period was not long enough.
To conclude, with atomic layer deposition technique, the implant surface roughness could control in nanoscale. Moreover, ZrO2 coatings showed better cell viability and alkaline phosphatase assay result than non-coating samples. Therefore, ZrO2 could be an appropriate coating material for improve osseointegration.
|Appears in Collections:||臨床牙醫學研究所|
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