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The establishment of animal population genetic information and the application of rapid nucleic acid detection platform for diagnosis of animal viral pathogens
Taiwan yellow cattle,Taiwan black goat,phylogenetic analysis,isothermal nucleic acid amplification,rapid disease diagnosis,
|Publication Year :||2019|
重組聚合酶擴增反應（recombinase polymerase amplification, RPA）為一個高敏感性且高專一性的恆溫擴增技術，反應溫度於37至42˚C，且僅需要簡單的樣品處理便可進行擴增反應，可在20分鐘內偵測10個copies的DNA或RNA標的。本研究第二部分成功應用此技術放大及偵測不同常見動物疾病之標的，包含山羊關節炎腦炎（caprine arthritis-encephalitis virus, CAEV）、牛白血病（bovine leukemia virus, BLV）及家禽白血病J型（avian leukosis virus subgroup J, ALV-J）；搭配側層流分析法（lateral flow dipstick, LFD）進行偵測，並與傳統現行主流疾病診斷方法進行比較。
The Genetic Preservation of Domestic Livestock was set to maintain an effective population size, preserve genetic diversity, genetic resources, and extend their utilization. In early times, characterizations of domestic livestock breeds were through observation of phenotypic heterogeneity, and could not justify accurately for the purpose of genetic improvement or to sustain the genetic resources preservation in Taiwan. The application of molecular genetics profiling provides advanced hereditary information for each individual, and thus enable us to better define the differences among livestock populations. The aim of this study was to characterize the current genetic structure of these Taiwan yellow cattle and Taiwan black goat populations, as well as to assess their phylogenetic relationship. The spatial distribution of the different breeds of cattle and goat populations and the high contribution of these populations to overall gene diversity supported the hypothesis that the present herd was derived from different genetic lineages that were incorporated to control the level of inbreeding. This information will be essential for establishing a reliable breeding and conservation plan and are also crucial for understanding the origin and breeding history of domestic cattle and goat breeds in Taiwan.
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal amplification technique, operating at 37-42˚C, with minimal sample preparation and capable of amplifying as low as 10 DNA or RNA target copies in about 20 min. We have used the technique to amplify diverse targets, including caprine arthritis-encephalitis virus (CAEV), bovine leukemia virus (BLV), and avian leukosis virus subgroup J (ALV-J) from a wide variety of target animals. Furthermore, different detection strategies integrated with RPA has been successfully performed using lateral flow strips detection. This study focuses on the comparison of different detection methodologies and targets related to RPA-LFD with other disease diagnostic technologies.
To sum up, livestock and poultry breeds are an important part of the biological genetic resources. They are renewable or changeable, and are influenced by the natural ecological environments and by social development. Understanding the origin and genetic phylogenetic relationship of domestic cattle and goat breeds are important for breeding management. Meanwhile, farmers and stockmen should know how to prevent, control and treat animal diseases through farm health planning and close working with vets. The developed RPA-LFD assays are simple and sensitive enough for point-of-care diagnostics. It also serves as a model platform that could be further adapted to detect other pathogens or genetic markers of interest using basic laboratory equipment. Animal husbandry would be better improved with more productive and healthy livestock and poultry in Taiwan.
|Appears in Collections:||動物科學技術學系|
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