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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡育彰(Yu-Chang Tsai) | |
dc.contributor.author | Han-Yi Wu | en |
dc.contributor.author | 吳函䭲 | zh_TW |
dc.date.accessioned | 2021-06-17T06:35:55Z | - |
dc.date.available | 2020-08-20 | |
dc.date.copyright | 2018-08-20 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-16 | |
dc.identifier.citation | 侯雅文. (2016). 水稻細胞分裂素訊息反應調節蛋白 OsRR6 與 OsRR11 之生理功能探討. 國立臺灣大學生物資源暨農學院農藝系碩士論文.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72332 | - |
dc.description.abstract | 隨著全球氣候變遷與全球暖化之影響,作物在非生物逆境下如乾旱、鹽害、冷害及熱逆境下的生長受到阻礙使產量下降,因此了解作物在非生物逆境下之反應機制及耐受性之相關研究亦趨重要。植物荷爾蒙調控著作物的生長發育,同時亦在非生物逆境下之反應機制扮演重要的角色,其中細胞分裂素之生合成及運輸於非生物逆境下皆會明顯地受到抑制,被認為是非生物逆境下之負向調控因子。細胞分裂素之反應是藉由雙分子訊息傳導系統 (two-component signaling system, TCS) 進行調控,其中在前人研究中顯示Type-A RRs能快速地受細胞分裂素誘導,被認為是細胞分裂素之原初反應因子,且在細胞分裂素訊息傳導中扮演負向調控之角色。水稻中Type-A OsRRs共有13個,其中OsRR6在前人研究中發現其基因表現量會明顯受到非生物逆境之誘導,考慮此基因家族間可能有基因功能之補償性,本試驗利用CRISPR/Cas9基因編輯之Osrr6/11突變株進行鹽害下OsRR6及OsRR11之生理功能探討。Osrr6/11突變株於鹽害逆境下有較野生型敏感之外表型,且相較於野生型有較多之鈉離子及過氧化氫累積之現象,其細胞膜穩定性及抗氧化酵素活性亦較野生型差,顯示OsRR6及OsRR11可能在鹽害逆境下參與地上部鈉離子之運輸及抗氧化逆境相關之機制調控中,並且在耐受性機制上扮演正向調控之角色。使用轉錄體定序 (RNA-seq) 進行全基因體表現分析發現OsRR6及OsRR11可能參與在細胞分裂素之活性調控、地上部鈉離子之運輸及與離層酸之生合成及訊息傳導之交互作用中。本試驗結果顯示OsRR6及OsRR11於鹽害逆境下,參與在離子平衡、抗氧化機制、細胞分裂素活性以及離層酸生合成與訊息反應,並扮演鹽害耐受性之正向調控因子。 | zh_TW |
dc.description.abstract | As the global warming and climate change is getting worse, abiotic stress such as drought, salinity, cold and heat stress usually limit growth development of crops and even reduce yield production. Therefore, understanding the response and the mechanism of crops under abiotic stress becomes more important. Plant hormones are key regulators of plant growth and development. Besides, they also play important roles in the response of abiotic stress in plants. Among them, the production and transportation of cytokinin, which is considered as a negative regulator of abiotic stress, is repressed after the stress treatments in previous studies. The responses of cytokinin are mediate by two-component signaling pathway. In cytokinin signaling pathway, the expression of type-A RR genes are rapidly induced by exogenous cytokinin and hence considered as cytokinin primary response genes. Some of type-A RRs in rice act as negative regulators in cytokinin signaling pathway. It has been known that there are 13 type-A OsRRs in rice and the gene expression of OsRR6 is significantly induced under several abiotic stress. Considering there may have redundant functions in type-A OsRRs, we use CRISPR/Cas9 genome editing Osrr6/11 mutants to study the physiological functions of OsRR6 and OsRR11 in salinity stress. In this study, Osrr6/11 double mutants had salt-sensitive phenotype, poor membrane stability, antioxidant enzyme activities and accumulated more sodium in shoot under salt stress. In addition, whole-genome gene expression profiling analysis showed that OsRR6 and OsRR11 may involve in cytokinin conjugation, sodium transportation and abscisic acid biosynthesis and signaling crosstalk in salt stress. These results show that OsRR6 and OsRR11 may be positive regulators in the response of salt stress in rice. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:35:55Z (GMT). No. of bitstreams: 1 ntu-107-R05621105-1.pdf: 7316188 bytes, checksum: b72fc32ce5d2078f49ceaec861bf0e2a (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書 I
致謝 II 摘要 III Abstract IV 目錄 V 圖目錄 VIII 表目錄 X 附表目錄 X 第一章 前人研究 1 1.1 植物於非生物逆境下之反應 1 1.2 植物於非生物逆境下之防禦機制 2 1.3 植物於非生物逆境下之訊息傳導路徑 2 1.4 非生物逆境下植物荷爾蒙間交互作用之關係 4 1.5 細胞分裂素於非生物逆境下之反應 4 1.6 水稻細胞分裂素雙分子訊息傳導系統 5 1.7 細胞分裂素訊息反應調節蛋白 Type-A Response Regulators 6 1.8 CRISPR/Cas9基因編輯Osrr6/11突變株之建立及特性分析 7 第二章 材料與方法 10 2.1 CRISPR/Cas9基因編輯Osrr6/11水稻突變株之建立 10 2.2 水稻植株之材料與生長條件 10 2.3 非生物逆境處理 11 2.4 相對離子滲漏度測定 11 2.5 相對水分含量測定 12 2.6 鈉鉀離子含量測定 12 2.7 過氧化氫含量測定 12 2.8 丙二醛 Malondialdehyde 含量測定 13 2.9 抗氧化酵素活性測定 13 2.10 滲透壓調節物質含量測定 14 2.11 RNA 萃取 14 2.12 RNA-seq 資料分析 15 2.13 統計分析方法 16 第三章 結果 17 3.1 Osrr6/11 突變株於乾旱逆境下之生理表現 17 3.2 Osrr6/11 突變株於冷害逆境下之生理表現 17 3.3 Osrr6/11 突變株於鹽害逆境下之生理表現 18 3.4 Osrr6/11突變株於鹽害下之細胞膜膜系傷害程度較野生型大 18 3.5 Osrr6/11突變株於鹽害下所受之氧化逆境相較於野生型嚴重 19 3.6 Osrr6/11突變株於鹽害下之抗氧化能力較野生型差 19 3.7 Osrr6/11突變株於鹽害下之滲透壓調節物質相較於野生型多 20 3.8 Osrr6/11突變株於鹽害下較野生型有較高之地上部鈉離子累積含量 20 3.9 Osrr6/11突變株於鹽害逆境下之全基因體表現分析 21 3.10 Osrr6/11突變株中差異表現基因 (DEGs) 之基因功能註解與分析 22 3.11 Osrr6/11突變株中差異表現基因 (DEGs) 之KEGG生物途徑分析 23 3.12 Osrr6/11突變株在細胞分裂素相關基因之基因表現情形 24 3.13 Osrr6/11突變株在離子轉運蛋白相關基因之基因表現情形 26 3.14 Osrr6/11突變株在抗氧化酵素相關基因之基因表現情形 27 3.15 Osrr6/11突變株在離層酸相關基因之基因表現情形 27 第四章 討論 28 4.1 OsRR6及OsRR11於不同非生物逆境下之生理功能探討 29 4.2 OsRR6及OsRR11為鹽害逆境下之正向調控因子 30 4.3 OsRR6及OsRR11於鹽害下調控細胞分裂素相關之基因表現 31 4.4 OsRR6及OsRR11參與在地上部鈉離子的運輸調控中 32 4.5 OsRR6及OsRR11於鹽害下參與ABA之相關反應機制 33 第五章 結論 35 參考文獻 36 | |
dc.language.iso | zh-TW | |
dc.title | 水稻細胞分裂素訊息反應調節蛋白 OsRR6 與 OsRR11 於鹽害逆境下之生理功能探討 | zh_TW |
dc.title | Functional Characterization of Rice Cytokinin Response regulators OsRR6 and OsRR11 in Salinity Stress | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張孟基(Men-Chi Chang),洪傳揚(Chwan-Yang Hong),陳良築(Liang-Jwu Chen),侯新龍(Shin-Lon Ho) | |
dc.subject.keyword | 水稻,細胞分裂素,訊息傳導,OsRR6,OsRR11,鹽害,轉錄體分析, | zh_TW |
dc.subject.keyword | Oryza sativa,cytokinin,signaling,OsRR6,OsRR11,salt stress,RNA-seq, | en |
dc.relation.page | 98 | |
dc.identifier.doi | 10.6342/NTU201802459 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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