請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72291
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 朱士維 | |
dc.contributor.author | Kuo-Jen Hsu | en |
dc.contributor.author | 徐國仁 | zh_TW |
dc.date.accessioned | 2021-06-17T06:33:38Z | - |
dc.date.available | 2018-08-20 | |
dc.date.copyright | 2018-08-20 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-16 | |
dc.identifier.citation | Abdelfattah, A. S., et al (2016). 'A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices.' The Journal of Neuroscience 36, 2458-2472.
Adams, M. D., et al (2000). 'The genome sequence of Drosophila melanogaster.' Science 287, 2185-2195. Ahrens, M. B., et al (2013). 'Whole-brain functional imaging at cellular resolution using light-sheet microscopy.' Nature Methods 10, 413-420. Alivisatos, A. P., et al (2012). 'The brain activity map project and the challenge of functional connectomics.' Neuron 74, 970-974. Arnold, C. B., et al (2013). Tunable acoustic gradient index of refraction lens and system, US Patents. Aso, Y., et al (2014). 'Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila.' eLife 3, e04580. Babcock, H. W. (1953). 'The possibility of compensating astronomical seeing.' Publications of the Astronomical Society of the Pacific 65, 229-236. Bahlmann, K., et al (2007). 'Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz.' Optics Express 15, 10991-10998. Beaurepaire, E., et al (2001). 'Ultra-deep two-photon fluorescence excitation in turbid media.' Optics Communications 188, 25-29. Beitel, G. J. and Krasnow, M. A. (2000). 'Genetic control of epithelial tube size in the Drosophila tracheal system.' Development 127, 3271-3282. Beliveau, V., et al (2016). 'A high-resolution in vivo atlas of the human brain's serotonin system.' The Journal of Neuroscience 37, 120-128. Belliveau, J. W., et al (1991). 'Functional mapping of the human visual cortex by magnetic resonance imaging.' Science 254, 716-719. Bewersdorf, J., et al (1998). 'Multifocal multiphoton microscopy.' Optics Letters 23, 655-657. Bocarsly, M. E., et al (2015). 'Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain.' Biomedical Optics Express 6, 4546-4556. Booth, M. J. (2014). 'Adaptive optical microscopy: the ongoing quest for a perfect image.' Light: Science & Applications 3, e165. Bouchard, M. B., et al (2015). 'Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms.' Nature Photonics 9, 113-119. BRC/NTHU. 'FlyCircuit.' from http://www.flycircuit.tw. Burghardt, A. J., et al (2011). 'High-resolution computed tomography for clinical imaging of bone microarchitecture.' Clinical Orthopaedics and Related Research 469, 2179-2193. Buzsáki, G., et al (2015). 'Tools for probing local circuits: high-density silicon probes combined with optogenetics.' Neuron 86, 92-105. Camillo Golgi, S. R. y. C. (1906). In recognition of their work on the structure of the nervous system. Nobel Prize Lecture. Chen, B., et al (2018). 'Rapid volumetric imaging with Bessel-Beam three-photon microscopy.' Biomedical Optics Express 9, 1992-2000. Chen, B.-C., et al (2014). 'Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.' Science 346, 1257998. Chen, T.-W., et al (2013). 'Ultra-sensitive fluorescent proteins for imaging neuronal activity.' Nature 499, 295-300. Chen, Z., et al (2012). 'Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores.' Optics Express 20, 18525-18536. Cheng, A., et al (2011). 'Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.' Nature Methods 8, 139-142. Cheng, L.-C., et al (2014). 'Measurements of multiphoton action cross sections for multiphoton microscopy.' Biomedical Optics Express 5, 3427-3433. Chiang, A. S., et al (2011). 'Three-dimensional reconstruction of brain-wide wiring networks in Drosophila at single-cell resolution.' Current Biology 21, 1-11. Chu, S.-W., et al (2003). 'In vivo developmental biology study using noninvasive multi-harmonic generation microscopy.' Optics Express 11, 3093-3099. Chudakov, D. M., et al (2010). 'Fluorescent proteins and their applications in imaging living cells and tissues.' Physiological Reviews 90, 1103-1163. Chung, K., et al (2013). 'Structural and molecular interrogation of intact biological systems.' Nature 497, 332-337. Dana, H., et al (2016). 'Sensitive red protein calcium indicators for imaging neural activity.' eLife 5, e12727. Davila, H. V., et al (1973). 'Large change in axon fluorescence that provides a promising method for measuring membrane-potential.' Nature: New Biology 241, 159-160. Davis, R. L. (2005). 'Olfactory memory formation in Drosophila: from molecular to systems neuroscience.' Annual Review of Neuroscience 28, 275-302. Day, R. N. and Davidson, M. W. (2009). 'The fluorescent protein palette: tools for cellular imaging.' Chemical Society Reviews 38, 2887-2921. Dean, K. M. and Fiolka, R. (2014). 'Uniform and scalable light-sheets generated by extended focusing.' Optics Express 22, 26141-26152. Denk, W., et al (1990). 'Two-photon laser scanning fluorescence microscopy.' Science 248, 73-76. Driscoll, J. D., et al (2011). 'Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy.' Journal of Neurophysiology 105, 3106-3113. Ducros, M., et al (2013). 'Encoded multisite two-photon microscopy.' Proceedings of the National Academy of Sciences 110, 13138-13143. Duocastella, M., et al (2017). 'Fast inertia-free volumetric light-sheet microscope.' ACS Photonics 4, 1797-1804. Duocastella, M., et al (2012). 'Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics.' Journal of Biomedical Optics 17, 050505. Duocastella, M., et al (2014). 'Simultaneous multiplane confocal microscopy using acoustic tunable lenses.' Optics Express 22, 19293-19301. Erwin Neher, B. S. (1991). For their discoveries concerning the function of single ion channels in cells. Nobel Prize Lecture. Espuny-Camacho, I., et al (2017). 'Hallmarks of Alzheimer’s disease in stem-cell-derived human neurons transplanted into mouse brain.' Neuron 93, 1066-1081. Fahrbach, F. O., et al (2013). 'Rapid 3D light-sheet microscopy with a tunable lens.' Optics Express 21, 21010-21026. Fenno, L., et al (2011). 'The development and application of optogenetics.' Annual Review of Neuroscience 34, 389-412. Fittinghoff, D. N. and Squier, J. A. (2000). 'Time-decorrelated multifocal array for multiphoton microscopy and micromachining.' Optics Letters 25, 1213-1215. Galison, P. (1997). Image and logic - A material culture of microphysics. Gias, C., et al (2005). 'Retinotopy within rat primary visual cortex using optical imaging.' NeuroImage 24, 200-206. Göbel, W., et al (2007). 'Imaging cellular network dynamics in three dimensions using fast 3D laser scanning.' Nature Methods 4, 73-79. Goense, J., et al (2016). 'fMRI at high spatial resolution: implications for BOLD-models.' Frontiers in Computational Neuroscience 10, 66. Gong, Y., et al (2015). 'High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.' Science 350, 1361-1366. Greenshaw, A. J. (1985). Electrical and chemical stimulation of brain tissue in vivo. General Neurochemical Techniques. 1: 233-277. Grewe, B. F., et al (2010). 'High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision.' Nature Methods 7, 399-405. Gu, M. (2000). Advanced optical imaging theory. Guven-Ozkan, T. and Davis, R. L. (2014). 'Functional neuroanatomy of Drosophila olfactory memory formation.' Learning & Memory 21, 519-526. Halpin, S. F. S. (2004). 'Brain imaging using multislice CT: a personal perspective.' The British Journal of Radiology 77, S20-S26. Hama, H., et al (2011). 'Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.' Nature Neuroscience 14, 1481-1488. Harada, H., et al (2006). 'Brain olfactory activation measured by near-infrared spectroscopy in humans.' The Journal of Laryngology & Otology 120, 638-643. Heisenberg, M. (2003). 'Mushroom body memoir: from maps to models.' Nature Reviews Neuroscience 4, 266-275. Helmchen, F. and Denk, W. (2005). 'Deep tissue two-photon microscopy.' Nature Methods 2, 932-940. Herculano-Houzel, S. (2014). 'The glia/neuron ratio: How it varies uniformly across brain structures and species and what that means for brain physiology and evolution.' Glia 62, 1377-1391. Hillman, E. M. C. (2007). 'Optical brain imaging in vivo: techniques and applications from animal to man.' Journal of Biomedical Optics 12, 051402. Hochbaum, D. R., et al (2014). 'All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.' Nature Methods 11, 825-833. Hoge, R. D., et al (2005). 'Simultaneous recording of task-induced changes in blood oxygenation, volume, and flow using diffuse optical imaging and arterial spin-labeling MRI.' NeuroImage 25, 701-707. Honegger, K. S., et al (2011). 'Cellular-resolution population imaging reveals robust sparse coding in the Drosophila mushroom body.' The Journal of Neuroscience 31, 11772-11785. Horton, N. G., et al (2013). 'In vivo three-photon microscopy of subcortical structures within an intact mouse brain.' Nature Photonics 7, 205-209. Hsu, K.-J., et al (2017). 'Optimizing depth-of-field extension in optical sectioning microscopy techniques using a fast focus-tunable lens.' Optics Express 25, 16783-16794. Hsu, K.-J., et al (2018). 'Whole-brain imaging and characterization of Drosophila brains based on one-, two-, and three-photon excitations.' eLifeunder review. Huang, C., et al (2018). 'Long-term optical brain imaging in live adult fruit flies.' Nature Communications 9, 872. Ignell, R., et al (2009). 'Presynaptic peptidergic modulation of olfactory receptor neurons in Drosophila.' Proceedings of the National Academy of Sciences 106, 13070-13075. Imai, T., et al (2014). A varifocal lens using an electrooptic KTa1−xNbxO3 crystal with a microsecond order response time. International Conference on Electronics Packaging (ICEP). Toyama, Japan, IEEE: 698-702. Ji, N. (2017). 'Adaptive optical fluorescence microscopy.' Nature Methods 14, 374-380. Jung, J. C., et al (2004). 'In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.' Journal of Neurophysiology 92, 3121-3133. Jung, S. Y., et al (2017). 'An anatomically resolved mouse brain proteome reveals parkinson disease-relevant pathways.' Molecular & Cellular Proteomics 16, 581-593. Kalueff, A. V., et al (2014). 'Zebrafish as an emerging model for studying complex brain disorders.' Trends in Pharmacological Sciences 35, 63-75. Kao, Y.-T., et al (2012). 'Focal switching of photochromic fluorescent proteins enables multiphoton microscopy with superior image contrast.' Biomedical Optics Express 3, 1955-1963. Keene, A. C. and Waddell, S. (2007). 'Drosophila olfactory memory: single genes to complex neural circuits.' Nature Reviews Neuroscience 8, 341-354. Khodagholy, D., et al (2014). 'NeuroGrid: recording action potentials from the surface of the brain.' Nature Neuroscience 18, 310-315. Kim, C. K., et al (2017). 'Integration of optogenetics with complementary methodologies in systems neuroscience.' Nature Reviews Neuroscience 18, 222-235. Kobat, D., et al (2009). 'Deep tissue multiphoton microscopy using longer wavelength excitation.' Optics Express 17, 13354-13364. Kobat, D., et al (2011). 'In vivo two-photon microscopy to 1.6-mm depth in mouse cortex.' Journal of Biomedical Optics 16, 106014. Kohl, J., et al (2013). 'A bidirectional circuit switch reroutes pheromone signals in male and female brains.' Cell 155, 1610-1623. Kong, L., et al (2015). 'Continuous volumetric imaging via an optical phase-locked ultrasound lens.' Nature Methods 12, 759-762. Koyama, D., et al (2011). 'Three-dimensional variable-focus liquid lens using acoustic radiation force.' IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control 58, 2720-2726. Levene, M. J., et al (2004). 'In vivo multiphoton microscopy of deep brain tissue.' Journal of Neurophysiology 91, 1908-1912. Lin, C.-W., et al (2015). 'Automated in situ brain imaging for mapping the Drosophila connectome.' Journal of Neurogenetics 29, 157-168. Lin, H.-H., et al (2013). 'Parallel neural pathways mediate CO2 avoidance responses in Drosophila.' Science 340, 1338-1341. Lin, Y.-Y., et al (2015). 'Three-wavelength light control of freely moving Drosophila melanogaster for less perturbation and efficient social-behavioral studies.' Biomedical Optics Express 6, 514-523. Liu, S. and Hua, H. (2011). 'Extended depth-of-field microscopic imaging with a variable focus microscope objective.' Optics Express 19, 353-362. Lo, C.-C. and Chiang, A.-S. (2016). 'Toward whole-body connectomics.' The Journal of Neuroscience 36, 11375-11383. Lu, R., et al (2017). 'Video-rate volumetric functional imaging of the brain at synaptic resolution.' Nature Neuroscience 20, 620-628. Lukyanov, K. A., et al (2005). 'Photoactivatable fluorescent proteins.' Nature Reviews Molecular Cell Biolology 6, 885-890. Marin, E. C., et al (2002). 'Representation of the glomerular olfactory map in the Drosophila brain.' Cell 109, 243-255. Matthews, P. M., et al (2006). 'Neuroimaging: applications of fMRI in translational medicine and clinical practice.' Nature Reviews Neuroscience 7, 732–744. McLeod, E. and Arnold, C. B. (2007). 'Mechanics and refractive power optimization of tunable acoustic gradient lenses.' Journal of Applied Physics 102, 033104. Medzhitov, R., et al (1997). 'A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.' Nature 388, 394-397. Megías, M., et al (2001). 'Total number and distribution of inhibitory and excitatory synapses on hippocampal CA1 pyramidal cells.' Neuroscience 102, 527-540. Mermillod-Blondin, A., et al (2008). 'High-speed varifocal imaging with a tunable acoustic gradient index of refraction lens.' Optics Letters 33, 2146-2148. Minsky, M. (1988). 'Memoir on inventing the confocal scanning microscope.' Scanning 10, 128-138. Nöbauer, T., et al (2017). 'Video rate volumetric Ca2+ imaging across cortex using seeded iterative demixing (SID) microscopy.' Nature Methods 14, 811-818. Novák, O., et al (2016). 'Immediate manifestation of acoustic trauma in the auditory cortex is layer specific and cell type dependent.' Journal of Neurophysiology 115, 1860-1874. Olivier, N., et al (2009). 'Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens.' Optics Letters 34, 1684-1686. Ouzounov, D. G., et al (2017). 'In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.' Nature Methods 14, 388-390. Packer, A. M., et al (2013). 'Targeting neurons and photons for optogenetics.' Nature Neuroscience 16, 805-815. Packer, A. M., et al (2015). 'Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo.' Nature Methods 12, 140-146. Pan, C., et al (2016). 'Shrinkage-mediated imaging of entire organs and organisms using uDISCO.' Nature Methods 13, 859-867. Pawley, J. B. (2006). Handbook of biological confocal microscopy, Springer US. Pedrazzani, M., et al (2016). 'Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain.' Journal of Biomedical Optics 21, 036006. Pelvig, D. P., et al (2008). 'Neocortical glial cell numbers in human brains.' Neurobiology of Aging 29, 1754-1762. Pongs, O., et al (1993). 'Frequenin - a novel calcium-binding protein that modulates synaptic efficacy in the Drosophila nervous-system.' Neuron 11, 15-28. Power, R. M. and Huisken, J. (2017). 'A guide to light-sheet fluorescence microscopy for multiscale imaging.' Nature Methods 14, 360-373. Prevedel, R., et al (2016). 'Fast volumetric calcium imaging across multiple cortical layers using sculpted light.' Nature Methods 13, 1021-1028. Prevedel, R., et al (2014). 'Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.' Nature Methods 11, 727-730. Quirin, S., et al (2014). 'Simultaneous imaging of neural activity in three dimensions.' Frontiers in Neural Circuits 8, 29. Quirin, S., et al (2013). 'Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging.' Optics Express 21, 16007-16021. Reddy, G. D., et al (2008). 'Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity.' Nature Neuroscience 11, 713-720. Reihani, N. and Oddershede, L. B. (2009). 'Confocal microscopy of thick specimens.' Journal of Biomedical Optics 14, 030513. Resendez, S. L., et al (2016). 'Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.' Nature Protocols 11, 566-597. Rivera, D. R., et al (2011). 'Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue.' Proceedings of the National Academy of Sciences 108, 17598-17603. Root, C. M., et al (2007). 'Propagation of olfactory information in Drosophila.' Proceedings of the National Academy of Sciences 104, 11826-11831. Ruta, V., et al (2010). 'A dimorphic pheromone circuit in Drosophila from sensory input to descending output.' Nature 468, 686-690. Saar, B. G., et al (2011). 'Coherent Raman scanning fiber endoscopy.' Optics Letters 36, 2396-2398. Scanziani, M. and Häusser, M. (2009). 'Electrophysiology in the age of light.' Nature 461, 930-939. Schrödel, T., et al (2013). 'Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light.' Nature Methods 10, 1013-1020. Shcherbakova, D. M. and Verkhusha, V. V. (2013). 'Near-infrared fluorescent proteins for multicolor in vivo imaging.' Nature Methods 10, 751-754. Sidiropoulou, K., et al (2006). 'Inside the brain of a neuron.' EMBO reports 7, 886-892. Smithpeter, C. L., et al (1998). 'Penetration depth limits of in vivo confocal reflectance imaging.' Applied Optics 37, 2749-2754. Song, A., et al (2017). 'Volumetric two-photon imaging of neurons using stereoscopy (vTwINS).' Nature Methods 14, 420-426. Sporns, O., et al (2005). 'The human connectome: a structural description of the human brain.' Plos Computational Biology 1, 245-251. Spors, H., et al (2006). 'Temporal dynamics and latency patterns of receptor neuron input to the olfactory bulb.' The Journal of Neuroscience 26, 1247-1259. Sullivan, S. Z., et al (2014). 'High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories.' Optics Express 22, 24224-24234. Szalay, G., et al (2016). 'Fast 3D imaging of spine, dendritic, and neuronal assemblies in behaving animals.' Neuron 92, 723-738. Taber, K. H., et al (2010). 'Optical imaging: a new window to the adult brain.' The Journal of Neuropsychiatry and Clinical Neurosciences 22, 357-360. Tainaka, K., et al (2014). 'Whole-body imaging with single-cell resolution by tissue decolorization.' Cell 159, 911-924. Takahashi, K., et al (2006). 'Transcranial fluorescence imaging of auditory cortical plasticity regulated by acoustic environments in mice.' European Journal of Neuroscience 23, 1365-1376. Tang, J., et al (2012). 'Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique.' Proceedings of the National Academy of Sciences 109, 8434-8439. Tao, X., et al (2017). 'Transcutical imaging with cellular and subcellular resolution.' Biomedical Optics Express 8, 1277-1289. Theer, P. and Denk, W. (2006). 'On the fundamental imaging-depth limit in two-photon microscopy.' Journal of the Optical Society of America A 23, 3139-3149. Theer, P., et al (2003). 'Two-photon imaging to a depth of 1000 µm in living brains by use of a Ti: Al2O3 regenerative amplifier.' Optics Letters 28, 1022-1024. Thériault, G., et al (2014). 'Extended two-photon microscopy in live samples with Bessel beams: steadier focus, faster volume scans, and simpler stereoscopic imaging.' Frontiers in Cellular Neuroscience 8, 139. Toronov, V. Y., et al (2007). 'A spatial and temporal comparison of hemodynamic signals measured using optical and functional magnetic resonance imaging during activation in the human primary visual cortex.' NeuroImage 34, 1136-1148. Tsai, D., et al (2017). 'A very large-scale microelectrode array for cellular-resolution electrophysiology.' Nature Communications 8, 1802. Tung, C. K., et al (2004). 'Effects of objective numerical apertures on achievable imaging depths in multiphoton microscopy.' Microscopy Research and Technique 65, 308-314. Tuthill, J. C. (2009). 'Lessons from a compartmental model of a Drosophila neuron.' The Journal of Neuroscience 29, 12033-12034. Vaquero, J. J. and Kinahan, P. (2015). 'Positron emission tomography: current challenges and opportunities for technological advances in clinical and preclinical imaging systems.' Annual Review of Biomedical Engineering 17, 385-414. Vargas, R., et al (2011). 'The zebrafish brain in research and teaching: a simple in vivo and in vitro model for the study of spontaneous neural activity.' Advances in Physiology Education 35, 188-196. Viscarra Rossel, R. A. and McBratney, A. B. (1998). 'Laboratory evaluation of a proximal sensing technique for simultaneous measurement of soil clay and water content.' Geoderma 85, 19-39. Waerzeggers, Y., et al (2010). 'Mouse models in neurological disorders: applications of non-invasive imaging.' Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1802, 819-839. Wang, C., et al (2014). 'Multiplexed aberration measurement for deep tissue imaging in vivo.' Nature Methods 11, 1037-1040. Wang, J. W., et al (2003). 'Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain.' Cell 112, 271-282. Wang, M., et al (2018). In vivo three-photon imaging of deep cerebellum. SPIE BiOS, SPIE. Wang, M., et al (2018). Comparison of excitation wavelengths for in vivo deep imaging of mouse brain. SPIE BiOS, SPIE. Wang, Y., et al (2004). 'Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fluorescent protein-based Ca2+ imaging.' The Journal of Neuroscience 24, 6507-6514. Wei, L., et al (2012). 'Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging.' Biomedical Optics Express 3, 1465-1475. White, J. G. (1987). 'An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.' The Journal of Cell Biology 105, 41-48. Wilt, B. A., et al (2009). 'Advances in light microscopy for neuroscience.' Annual Review of Neuroscience 32, 435-506. Yang, W., et al (2016). 'Simultaneous multi-plane imaging of neural circuits.' Neuron 89, 269-284. Zong, W., et al (2014). 'Large-field high-resolution two-photon digital scanned light-sheet microscopy.' Cell Research 25, 254-257. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72291 | - |
dc.description.abstract | 自從 Cajal 利用光學顯微鏡觀察神經組織開始,科學家已累積大量單一神經細 胞或是數個神經細胞的知識,但這仍不足讓我們全盤了解大腦的功能。大腦是由 數以千萬億微米等級的神經細胞形成的複雜三維網路,彼此間利用毫秒時間尺度 的動態行為運作。因此要了解大腦的功能,必須要發展適合的工具,利用非侵入 式的方式,在具有單一神經細胞的解析度下同時觀察活體全腦功能。
要研究活體全腦功能,傳統電生理學可量測毫秒解析度的神經活動,而功能 性腦磁振造影可非侵入式觀察人的全腦。但電生理學為侵入式觀察,且同時只能 觀察數量有限的細胞,而功能性腦磁振造影的解析度不足以觀察單一神經細胞, 且無法提供腦活動的直接結果。而光學方法可以以非侵入式觀察並提供單一神經 細胞解析度,且可以觀察小動物的全腦,是活體腦功能研究的最佳工具。本篇論 文中,由於果蠅完備的解剖腦圖譜,因此我們用果蠅做為研究對象。 利用光學方法研究活體腦功能時,常見的工具為共軛焦及雙光子顯微鏡,因 其具備光學切片能力,可應用於組織觀察。但切片限制了快速三維取像的速度, 本篇論文提出結合聲光變焦透鏡的商用雙光子顯微鏡的方法,提升三維取像速度, 它以數十萬至百萬赫茲的頻率做軸向的焦點掃描,掃描範圍達上百微米,結合掃 描系統的橫向任意曲線掃描,提供了一個三維光學緞帶成像的掃描方式。利用這 個新穎的成像模式可以觀察毫秒時間尺度的三維神經活動,且同時避免活體樣本 晃動造成的影響,對於高神經密度的果蠅腦是最佳的研究工具。 而在研究果蠅腦功能時,發現了另一個預料外的現象,就是雙光子顯微鏡並 無法穿透整個約兩百微米深的果蠅全腦,其主要原因為腦中氣管強烈的像差及散 射。為了讓光學影像能穿透果蠅全腦,本篇論文中利用了波長 1300 奈米的雷射, 搭配三光子螢光激發,首次實現了活體果蠅在亞細胞解析度下的全腦觀察。由於 長波長的光可以減少散射,並且同時降低波前誤差產生的像差,而三光子螢光提 供了相較於雙光子螢光更優異的光學切片能力。另外,我們也首次利用不同波長 的激發,定量的驗證並解釋限制雙光子影像在果蠅腦穿透深度的機制。在短波長激發下,散射是主要限制穿透深度的因素,然而,在長波長的激發下,像差的影 響超越了散射,成為主要限制影像穿透深度的原因。 藉由以上高速及深組織的光學顯微技術開發,以及全面性的了解果蠅腦的光 學特性,完成果蠅功能腦圖譜的目標指日可待。 | zh_TW |
dc.description.abstract | Since the day of Cajal, neuroscientists have accumulated significant amount of knowledge of single neuron or few-neuron circuits. However, to understand the emergent properties of brain, which composed of three-dimensional (3D) networks from thousands to millions of micron-sized neurons with millisecond to second temporal dynamics, suitable tools should be adopted to explore the functional dynamics throughout whole living brain with single neuron spatiotemporal resolution, i.e., functional connectome.
To study functional connectome, electrophysiology has been successfully applied to single neuron measurements with millisecond resolution in an intact brain, and functional magnetic resonance imaging (fMRI) has been widely used to study whole human brain functional properties. However, electrophysiology is invasive, and the number of simultaneously monitored neurons is limited, while fMRI provides only indirect results of brain activities and nonsufficient spatiotemporal resolution to distinguish single neuron. On the other hand, optical methods provide noninvasive measurements, high spatiotemporal resolution to distinguish single neuron, and whole-brain observation when applied to small animal brains, is the optimal tool. In this dissertation, Drosophila is selected as our research target due to its nearly-complete anatomical connectome. When using optical method to study the brain, confocal/two-photon microscope (2PM) is widely adopted due to their sectioning capability, which is suitable for tissue inspection. However, their 3D acquisition speeds are limited due to sectioning. In this dissertation, we enhance 3D acquisition speed by integrating an ultrasound lens (UL) with a commercial 2PM, providing hundreds of kHz to one MHz axial scan rate with more than 100 μm axial extent. Combined with a commercial scanner that allows arbitrary curve scan on lateral plane, a novel ribbon scan imaging modality is developed. It is demonstrated to monitor millisecond temporal dynamics of 3D neurons of interest without motion artifacts, which is best suited for densely-packed Drosophila brain. During Drosophila brain functional studies, it is unexpectedly discovered that 2PM cannot penetrated the whole ~ 200 μm living brain. The reason is the extraordinary strong aberration/scattering from the tracheae structures. To improve imaging depth, a 1300-nm laser combined with three-photon excitation (3PE) is adopted to achieve whole-brain observation with subcellular resolution for the first time. The long excitation wavelength simultaneously reduces scattering, and aberration caused by phase error. In addition, 3PE process renders exceptional optical section capability. To explore the mechanism that limit two-photon imaging depth in Drosophila brains, the brain optical properties at various excitation wavelengths are quantitatively characterized for the first time. Surprisingly, at short wavelength, scattering dominates; while aberration exceeds it at long wavelengths and becomes the main impeding factor of whole-brain observation in a living Drosophila. Through the validations of the 3D high-speed and deep-tissue optical imaging techniques, together with comprehensive understanding of light interaction in Drosophila brains, it paves the way toward constructing the first whole-Drosophila-brain functional connectome. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:33:38Z (GMT). No. of bitstreams: 1 ntu-107-D03222010-1.pdf: 159102264 bytes, checksum: a6340b5dc1af775cbc1afb8a3e87f9d8 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 中文摘要 II
ABSTRACT IV CONTENTS VI CHAPTER 1. INTRODUCTION 1 1.1 WHY STUDY IN VIVO BRAIN FUNCTIONS 1 1.2 CONVENTIONAL METHODS: IN VIVO, INADEQUATE RESOLUTION, WHOLE-BRAIN OBSERVATION 2 1.3 OPTICAL METHODS: IN VIVO, HIGH RESOLUTION, LIMITED PENETRATION DEPTH 6 1.4 RESEARCH TARGET AND CHALLENGES OF OPTICAL METHODS 10 1.4.1. Research target - Drosophila 10 1.4.2. Challenges of optical methods applied to Drosophila brain 12 1.5 GOALS 17 CHAPTER 2. OPTICAL METHODS FOR IN VIVO BRAIN FUNCTIONAL STUDIES 19 2.1 HISTORICAL REVIEWS 19 2.2 HIGH-SPEED 3D OPTICAL ACQUISITION TECHNIQUES 21 2.2.1. Wide-field imaging 21 2.2.2. Multifocal microscopy 28 2.2.3. Sparse sampling 32 2.2.4. Spatiotemporal multiplexing 37 2.2.5. Extended depth-of-field 39 2.2.6. Brief summary of high-speed imaging techniques 44 2.3 DEEP-TISSUE OPTICAL IMAGING TECHNIQUES 47 2.3.1. High-energy lasers 47 2.3.2. Photo-activatable fluorescence 50 2.3.3. Photon counting 52 2.3.4. Optical microendoscopy by gradient-index lens 56 2.3.5. Adaptive optics 58 2.3.6. Long wavelengths 61 2.3.7. Brief summary of deep-tissue imaging techniques 71 CHAPTER 3. OPTIMIZING DEPTH-OF-FIELD EXTENSION BY ULTRASOUND LENS 74 3.1 MOTIVATION BASED ON LITERATURE REVIEW 74 3.2 ULTRASOUND LENS POSITION VS. EXCITATION DEPTH-OF-FIELD EXTENSION 76 3.3 ULTRASOUND LENS POSITION VS. SIGNAL COLLECTION EFFICIENCY 82 3.4 DEPTH-OF-FIELD EXTENSION COMBINING BOTH EXCITATION AND SIGNAL COLLECTION 90 3.5 DEPTH-OF-FIELD EXTENSION BY CONSIDERING RESONANCE OF ULTRASOUND LENS 93 3.6 EXPERIMENTAL RESULTS 97 3.6.1. Setup 97 3.6.2. Experimental protocols 98 3.6.3. Extended depth-of-field measurements 99 3.6.4. Simultaneous imaging of fluorescent beads in 3D space 101 3.7 DISCUSSIONS AND COMPARISONS OF LITERATURES 102 CHAPTER 4. LIVING BRAIN FUNCTIONAL STUDY BY EXTENDED DEPTH-OF-FIELD 106 4.1 MOTIVATION: WHOLE-BRAIN OBSERVATION WITH SUBCELLULAR RESOLUTION 106 4.2 EXPERIMENTAL SETUP AND PERFORMANCES 109 4.3 3D IMAGE RECONSTRUCTION 114 4.4 SAMPLE PREPARATION 118 4.5 EXPERIMENTAL RESULTS 120 4.5.1. Experimental protocols 120 4.5.2. Volume imaging of odor-stimulated brain 126 4.5.3. Millisecond ribbon imaging on mushroom body 130 4.6 DISCUSSIONS 138 CHAPTER 5. WHOLE-BRAIN IMAGING AND OPTICAL CHARACTERIZATIONS 140 5.1 MOTIVATION: NO REPORT OF IN VIVO WHOLE-DROSOPHILA-BRAIN IMAGING 140 5.2 COMPARING IMAGING DEPTHS BY DIFFERENT EXCITATION WAVELENGTHS 144 5.3 WHOLE-BRAIN IMAGING AND OPTICAL CHARACTERIZATIONS 150 5.4 DISCUSSIONS 161 CHAPTER 6. CONCLUSIONS AND OUTLOOKS 163 6.1 HIGH-SPEED 3D IMAGING BY ULTRASOUND LENS AND OUTLOOKS 163 6.2 OPTICAL PROPERTIES CHARACTERIZATIONS, WHOLE-BRAIN OBSERVATIONS AND OUTLOOKS 164 FIGURE LIST 166 TABLE LIST 169 REFERENCES 170 | |
dc.language.iso | en | |
dc.title | 高速及深組織光學顯微技術應用於果蠅腦功能之研究 | zh_TW |
dc.title | High-speed and Deep-tissue Optical Microscope Techniques for Drosophila Brain Functional Studies | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 孫啟光,陳摘文,林元堯,林彥穎,吳順吉 | |
dc.subject.keyword | 多光子顯微鏡,快速三維光學緞帶成像,功能型腦圖譜,全腦觀察,訊號衰減,綠螢光蛋白, | zh_TW |
dc.subject.keyword | multiphoton microscopy,fast 3D optical ribbon imaging,functional connectome,whole-brain observation,signal attenuations,green-fluorescence protein, | en |
dc.relation.page | 185 | |
dc.identifier.doi | 10.6342/NTU201803368 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-16 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理學研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 155.37 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。