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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳示國(Shih-Kuo Chen) | |
dc.contributor.author | I-Ling Hsiao | en |
dc.contributor.author | 蕭亦聆 | zh_TW |
dc.date.accessioned | 2021-06-17T06:31:54Z | - |
dc.date.available | 2025-11-12 | |
dc.date.copyright | 2020-11-13 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-11-11 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72260 | - |
dc.description.abstract | 哺乳動物位於下視丘的視交叉上核(SCN),會產生約 24 小時節律的指令並影響生理功能像是睡眠清醒週期,並同步各部位的周邊時鐘,所以被視為是生理時鐘的中央節律點。眼睛內視網膜的自主感光視網膜神經節細胞(ipRGCs)可以將外界的光訊息傳遞到視交叉上核,調節晝夜節律的同步,又稱作光同步生理時鐘(circadian photoentrainment)。而過去行為實驗的結果顯示,晝夜節律的相移(phase shift)程度會受到2個因子影響:接受到的光子數量和內生性生理時間。然而,自主感光視網膜神經節細胞和視交叉上核之間的神經連結以及光同步生理時鐘的機制目前仍未知。根據目前的研究,視交叉上核的腹外側(ventrolateral part)是主要接受來自自主感光視神經細胞所傳遞光線訊息的區域。有趣的是,最近的研究顯示自主感光視網膜神經節細胞也可以直接投射到視交叉上核的背側(dorsal part)。利用不同方向的光線和 c-Fos 免疫螢光染色,我們發現視交叉上核的背側和腹側的神經細胞可以分別不同方向的光線活化,且在相同亮度的情況下,不同方向的光線可以引發不同程度的相移。總結來說,視交叉上核內不同區域的神經細胞可以被來自不同方向的光線活化,進而影響晝夜節律的同步。 | zh_TW |
dc.description.abstract | In mammals, the central circadian pacemaker suprachiasmatic nucleus (SCN) in the hypothalamus plays an important role to synchronize the peripheral clocks in a roughly 24-hour cycle and influence many physiological functions such as sleep-wake cycle. In addition, the SCN receives retinal inputs from the photoreceptors, intrinsically photosensitive retinal ganglion cells (ipRGCs), to mediate circadian photoentrainment. Behavioral tests indicated that circadian phase-shift response is determined by two factors: number of photons and circadian time. However, the neural connection from ipRGCs to the SCN remain poorly understood. Previous studies suggested that the ventrolateral SCN is the primary recipient region of photic input. Intriguingly, recent studies showed that the dorsal SCN may receive synaptic inputs from ipRGCs directly despite the physiological function is still unclear. Using different direction of light and c-Fos staining, here we showed that ipRGCs may activate the dorsal and ventral SCN differentially. Surprisingly, the phase shifts display significant differences with our different direction of light under the same illuminance. These results indicate the possibility that direction of light may be an effective factor to determine circadian phase response. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:31:54Z (GMT). No. of bitstreams: 1 U0001-1309202014451600.pdf: 8080549 bytes, checksum: bdf7067d8bbbfd237cdfbedfbab54128 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 謝誌 (i) 摘要 (iii) Abstract (iv) Contents (vi) Chapter I. Introduction (1) 1.1 Intrinsically photosensitive retinal ganglion cell (ipRGC) (1) 1.1.1 Properties of ipRGCs (2) 1.1.2 Subtypes of ipRGCs (2) 1.1.3 Projections of ipRGCs (3) 1.1.4 Development of ipRGCs (4) 1.2 Suprachiasmatic nucleus (4) 1.2.1 Diversity of the SCN neurons (5) 1.2.2 Inputs of the SCN (5) 1.2.3 Coupling of the SCN (7) 1.2.4 Outputs of the SCN (8) 1.3 Circadian photoentrainment (8) 1.3.1 Synchronization and entrainment (9) 1.3.2 Free-running period (tau) (10) 1.3.3 Phase response curve (PRC) (11) 1.3.4 Calcium and photoentrainment (12) Statement of the Purpose (14) Chapter II. Materials and Methods (15) 2.1 Animals (15) 2.2 Genotyping (15) 2.2.1 DNA extraction (16) 2.2.2 Polymerase chain reaction (PCR) and gel electrophoresis (16) 2.3 Lighting design (17) 2.4 c-Fos protein detection (17) 2.4.1 Histology and immunohistochemistry (17) 2.4.2 Image collection and measurement of c-Fos-positive cell counting (18) 2.5 Tamoxifen and 4-Hydroxytamoxifen injection (19) 2.5.1 Drug preparation (19) 2.5.2 Time-course of TRAPing with tamoxifen (20) 2.5.3 Time-course of TRAPing with 4-OHT (21) 2.6 Phase shifting response and data analysis (22) 2.6.1 Wheel-running activity (22) 2.6.2 Statistical analysis (23) Chapter III. Results (24) 3.1 Light from below may activate less neurons in the ventral SCN than light from above under same illuminance (24) 3.2 Different direction of light activate the SCN neurons in TRAP2 mice (27) 3.3 Light from below would generate smaller phase shift response than light from above under same illuminance during subjective night (28) Chapter IV. Discussion (31) 4.1 Anesthesia of avertin depress neuronal activity in the SCN (31) 4.2 Different direction of light can activate different regions of the SCN (32) 4.3 Direction of light may be an effective factor to influence the extent of circadian phase response (33) Chapter V. Significance of work (38) Appendix I. Oral Presentation (66) Appendix II. Poster (67) References (71) Figure 1. Schematic representation of the mouse genetic lines (39) Figure 2. Experimental design of light-induced c-Fos and CT point of light exposure procedure (40) Figure 3. Light-induced c-Fos expression in the SCN under different direction of light with the treatment of anesthetic avertin at CT16 (41) Figure 4. Light-induced c-Fos expression in the SCN under different direction of light without the treatment of anesthetic avertin at CT16 (42) Figure 5. Light-induced c-Fos expression in the SCN under different direction of light without the treatment of anesthetic avertin at CT22 (43) Figure 6. Heatmap showing the expression patterns of c-Fos under different direction of light and circadian time (44) Figure 7. Light-induced c-Fos expression in the dorsal and ventral SCN respectively under different direction of light at CT16 (45) Figure 8. Light-induced c-Fos expression in the dorsal and ventral SCN respectively under different direction of light at CT22 (46) Figure 9. Timeline of TRAPing with tamoxifen (47) Figure 10. Light-induced TRAP2 recombination and c-Fos expression in the SCN under different direction of light at CT22 (48) Figure 11. Light-induced c-Fos expression in the dorsal and ventral SCN respectively under different direction of light at CT22 in TRAP2 mice (49) Figure 12. Number of TRAPed cells and c-Fos positive cells under different light conditions at CT22 (50) Figure 13. Timeline of TRAPing with 4-OHT (51) Figure 14. Light-induced TRAP2 recombination with 4-OHT and c-Fos expression in the SCN under direction of light from above at CT22 (52) Figure 15. Number of TRAPed cells with 4-OHT and c-Fos positive cells under different light conditions at CT22 (53) Figure 16. Representative images of double-plotted wheel running behavior test in WT mice exposed to a light pulse from different direction (54) Figure 17. Total counts of locomotor activity under different direction of light (55) Figure 18. Free-running period under different direction of light (56) Figure 19. Phase response curve of light from below and light from below in WT mice (57) Figure 20. Representative images of double-plotted wheel running behavior test in WT mice exposed to a light pulse from different direction randomly (58) Figure 21. Total counts of locomotor activity under different direction of light randomly (59) Figure 22. Free-running period under different direction of light randomly (60) Figure 23. Phase response curve of light from below and light from below in WT mice using polynomial regression curve fitting (61) Figure 24. Sine wave analysis showing the amplitude of the curve of light from above and light from below randomly from CT10 to CT24 (62) Table 1. List of primers for genotyping (63) Table 2. List of antibody for immunohistochemistry (64) Table 3. List of the drugs (65) | |
dc.language.iso | en | |
dc.title | 視交叉上核編碼空間上的訊息來調節光同步生理時鐘 | zh_TW |
dc.title | Suprachiasmatic Nucleus Encodes Spatial Information for Circadian Photoentrainment | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張芳嘉(Fang-Chia Chang),姚皓傑(Hau-Jie Yau),黃雯華(Wendy W. Hwang-Verslues) | |
dc.subject.keyword | 視交叉上核,自主感光視網膜神經節細胞,光同步生理時鐘,相移, | zh_TW |
dc.subject.keyword | suprachiasmatic nucleus (SCN),intrinsically photosensitive retinal ganglion cells (ipRGCs),circadian photoentrainment,phase shift, | en |
dc.relation.page | 81 | |
dc.identifier.doi | 10.6342/NTU202004209 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-11-12 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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