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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 劉?睿 | |
dc.contributor.author | Tzu-Hsuan Li | en |
dc.contributor.author | 李姿璇 | zh_TW |
dc.date.accessioned | 2021-06-17T06:31:44Z | - |
dc.date.available | 2023-08-19 | |
dc.date.copyright | 2018-08-19 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-16 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72257 | - |
dc.description.abstract | 目前益生菌的研究主要專注在腸道功能的保健。一般認為益生菌夠維持腸道菌相,並且強化寄主腸道皮膜屏障。先前的研究發現經由攝食Bacillus licheniformis CK1添加的飼料能夠改善黴菌毒素在豬隻上所造成的生理障礙。而 Lactobacillus reuteri Pg4可增厚雞隻腸道黏膜及增加微絨毛的高度。然而,CK1及Pg4 對於腸道上皮細胞的影響機制並未有更深的研究。從in vitro實驗中發現 Pg4及CK1對於腸道上皮細胞有不同的助益機制。在Caco-2及IPEC-1單層膜細胞上,我們發現Pg4透過增加ZO-1及occludin等腸道上皮細胞緊密連接蛋白的表現,來幫助維持腸道單層膜的穩定性,進而延緩病原菌造成的傷害。而 CK1 則能夠顯著降低 Salmonella enterica serovar Typhimurium及Escherichia coli O157:H7腸道病原菌於IPEC-1及Caco-2細胞的吸附能力。我們的結果顯示了益生菌與寄主腸上皮細胞的交互作用,以及益生菌與病原菌之間可能的競爭機制。而本實驗利用分別來自豬隻及人類的上皮細胞模型探討CK1及Pg4可能的益生特性,可以提供菌株更多元的應用方向,結果也發現CK1及Pg4擁有各自優秀的益生特性,未來可以從複合益生菌的角度來研究在人類營養補充品或是飼料添加物的應用。 | zh_TW |
dc.description.abstract | The research related to probiotics has primarily been focused on improving intestine health, including the maintenance of the intestinal microbiota and modulation of host intestinal barrier integrity. As reported, by feeding with Bacillus licheniformis CK1, the reproductive disorder of pig caused by zearalenone toxin can be ameliorated. The other putative probiotic, like Lactobacillus reuteri Pg4, has been demonstrated to thicken the mucus layer and increase the height of microvilli, indicating that Pg4 enhances the function of host intestinal barrier. However, the detail of mechanisms for CK1 and Pg4 regulation in the barrier function remains unclear. Here, we showed that CK1 and Pg4 possess different probiotic properties, which are related to inhibit the adhesive ability of pathogens and enhance the barrier function of intestinal cells, respectively. These results indicate that CK1 reduces the amounts of pathogens, including Salmonella enterica serovar Typhimurium and Escherichia coli O157:H7, binding to the epithelial cells. Furthermore, Pg4 elevates the expression of ZO-1 and occludin, which contributes to the stability of intestinal monolayer and prevents the disruption from pathogen infection. In this study, we demonstrated that how CK1 and Pg4 are likely to function with epithelial monolayer model, and the competitive interaction with pathogen. In conclusion, both CK1 and Pg4 have their own novel properties for helping intestinal cells. Therefore, CK1 and Pg4 provide a perspective of combination of these probiotics which may apply in human dietary supplement or animal feed additives. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:31:44Z (GMT). No. of bitstreams: 1 ntu-107-R03642004-1.pdf: 2015649 bytes, checksum: ddf5144c49002e914748e75c5278a701 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 目錄
誌謝 I 中文摘要 III Abstract IV 圖目錄 VIII 壹、探討文獻 1 一、 腸道皮膜屏障 1 (一)腸道上皮細胞 1 (二)緊密連接蛋白 1 二、 腸道上皮細胞模型 2 三、 常見腸道病原菌 3 四、 益生菌種類 4 (一)Lactobacillus乳酸桿菌簡介 4 (二)Bacillus芽孢桿菌簡介 5 五、 益生菌與病原菌的交互作用 6 六、 研究動機 7 貳、材料方法 8 一、 菌株活化與細胞培養 8 (一)菌株置備 8 (二)細胞株活化、繼代培養及冷凍保存 8 二、 探究活菌對於細胞活性的影響 9 三、 探究CK1及Pg4潛力益生菌對細胞單層膜之影響 10 (一)建立細胞單層膜細胞模型 10 (二)CK1及Pg4潛力益生菌與病原菌共培養處理 10 (三)CK1及Pg4預處理保護由病原菌引起之單層膜破壞效應 11 (四)ZO-1及occludin細胞緊密蛋白之細胞免疫染色分布 11 (五)ZO-1及occludin細胞緊密蛋白之表現分析 12 四、 菌株對於腸道吸附性競爭力分析 13 叁、結果 15 一、 細胞活性分析 15 二、 CK1及Pg4潛力益生菌細菌對於腸道上皮細胞單層膜影響 17 (一)上皮細胞單層膜之建立 17 (二)CK1及Pg4潛力益生菌對於上皮細胞單層膜TEER值影響評估 17 (三)CK1及Pg4 潛力益生菌保護由病原菌引起之單層膜破壞效應 17 (四)CK1及Pg4潛力益生菌對於ZO-1及occludin細胞緊密連接蛋白表現評估 18 三、 CK1、Pg4與病原菌競爭吸附關係探討 20 肆、討論 22 一、 Pg4潛力益生菌增強腸道上皮細胞ZO-1及occludin緊密連接蛋白表現 22 二、 Pg4潛力益生菌具有保護上皮細胞完整性功能 23 三、 CK1及Pg4潛力益生菌降低病原菌吸附於腸道上皮細胞 26 四、 CK1潛力益生菌可能作用機制探討 27 五、 結論 28 參考文獻 42 | |
dc.language.iso | zh-TW | |
dc.title | Bacillus licheniformis CK1及Lactobacillus reuteri Pg4之潛力益生性研究 | zh_TW |
dc.title | Study of probiotic potentials of Bacillus licheniformis CK1 and Lactobacillus reuteri Pg4 | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡孟勳,謝建元,彭及忠 | |
dc.subject.keyword | 腸道皮膜完整性,Bacillus licheniformis CK1,Lactobacillus reuteri Pg4,吸附性競爭, | zh_TW |
dc.subject.keyword | intestinal barrier integrity,Bacillus licheniformis CK1,Lactobacillus reuteri Pg4,competition for adhesion, | en |
dc.relation.page | 50 | |
dc.identifier.doi | 10.6342/NTU201803696 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
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