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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 童心欣(Hsin-hsin Tung) | |
| dc.contributor.author | Yu-Chang Chou | en |
| dc.contributor.author | 周鈺錩 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:23:37Z | - |
| dc.date.available | 2020-11-13 | |
| dc.date.copyright | 2020-11-13 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-10-07 | |
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Y. 2017. “Geographic distribution of cadmium and its interaction with the microbial community in Longjiang River: risk evaluation after a shocking pollution accident.” Scientific Reports, 7:227. 土壤及地下水污染整治法. 民99年2月3日. 行政院環境保護署, 底泥污染來源及傳輸模式調查計畫-以重點河川為例. 行政院環境保護署: 2013. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72105 | - |
| dc.description.abstract | 本研究探討以底泥微生物菌群變化作為銅污染源鑑識之可行性。研究方法以模擬河川底泥反應槽進行分析，以河水添加不同濃度硫酸銅連續暴露底泥的方式進行研究。反應槽取用老街溪河水及底泥，於河水中添加硫酸銅作為進流，每周定時採取進出流分析，並於第0、14、28、56天取底泥樣品，對其萃取出的核酸進行次世代定序，檢測微生物菌群變化。藉由相關性分析及樣品微生物族群受底泥模擬接觸反應槽環境條件影響分析各自找出了與銅濃度具有相關性之菌屬，將兩者結果比對後篩選出之菌屬分別為Levilinea、Ornatilnea、Longilinea、Methylocystis、Mycobacterium、Sulfuricella、Candidatus Cloacamonas、Ignavibacterium、以及Subdivision3_genera_incertae_sedis此9屬。此9菌屬有機會以其族群變化作為銅污染鑑識技術的可能性。未來建議增加現地試驗驗證後可利用其消長變化作為鑑識污染來源的指標。 | zh_TW |
| dc.description.abstract | This study investigates the possibility of applying microbial community profile changes of sediments to copper pollution source identification. Sediment microcosm monitoring was used in this study. In the sediment microcosm, river water spiked with different concentrations of copper sulfate was used as the influent and cultured for 8 weeks. Water samples (influents and effluents) were collected periodically, and sediments were collected at 0, 2nd, 4th, and 8th weeks. And, crude nucleic acids in sediments were also extracted, cleaned and sent for 16S r-RNA gene amplicon sequencing by next generation sequencing with MiSeq platform (Illumina). The microbial sequence results from the sediment microcosms with Pearson correlation analysis and canonical correspondence analysis, showed that the abundance of Levilinea, Ornatilnea, Longilinea, Methylocystis, Mycobacterium, Sulfuricella, Candidatus Cloacamonas, Ignavibacterium and Subdivision3_genera_incertae_sedis were correlated with copper concentration in microcosm. These families were possible candidates for copper pollution source tracking in rivers or streams. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:23:37Z (GMT). No. of bitstreams: 1 U0001-0510202012403200.pdf: 4734596 bytes, checksum: d397a793060729fc7241823e407dd4d0 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 口試委員會審定書 I 誌謝 II 摘要 III Abstract IV 目錄 V 圖目錄 VIIII 表目錄 XII 第一章 前言 1 1.1 研究背景 1 1.2 研究目的 2 第二章 文獻回顧 3 2.1 底泥重金屬 3 2.2 底泥銅污染現狀 3 2.3 底泥微生物 4 2.4 銅污染影響微生物變化 5 2.5 底泥微生物族群變化 7 2.6 高通量定序應用於受污染底泥中微生物分析 8 第三章 研究方法與材料 10 3.1 實驗架構 10 3.2 模擬底泥接觸反應槽裝置以及樣本採集 12 3.2.1 現地採樣 12 3.2.2 反應槽設置 12 3.2.3 水樣、底泥採樣頻率 14 3.3 pH值、導電度、濁度測量 14 3.4 水中總有機碳分析(Total Organic Carbon, TOC) 15 3.5 水中銅濃度分析 15 3.6 水中硫酸鹽分析 15 3.7 水中氨氮分析 16 3.8 水中正磷酸鹽分析 16 3.9 底泥之銅濃度分析 16 3.10 底泥之有機質含量分析 18 3.11 反應槽底泥樣品核酸萃取及前處裡 19 3.12 高通量次世代定序 20 3.12.1 聚合酶連鎖反應 (PCR) 21 3.12.2 高通量定序 (high throughput sequencing) 22 3.12.3 序列整理 (reads pre-processing) 22 3.12.4 操作分類單元(OTU)分類與菌種分析 22 3.12.5 微生物族群組成百分比分析 23 3.13 統計方法(相關性分析) 23 3.14 樣品微生物族群受底泥模擬接觸反應槽環境條件影響分析 24 第四章 實驗結果 25 4.1 反應槽進出流水質參數 25 4.1.1 pH值 25 4.1.2 導電度 26 4.1.3 濁度 28 4.1.4 總有機碳 29 4.1.5 銅濃度 30 4.1.6 硫酸鹽 31 4.1.7 氨氮 32 4.1.8 正磷酸鹽 33 4.2 反應槽底泥參數 34 4.2.1 底泥累積銅濃度 34 4.2.2 有機質含量 36 4.3 高通量次世代定序 37 4.3.1 相關性分析 38 4.3.2 樣品微生物族群受底泥模擬接觸反應槽環境條件影響分析 42 第五章 結果討論 52 5.1 反應槽進出流水質參數 52 5.2 反應槽底泥參數 53 5.3 高通量次世代定序 54 第六章 結論與建議 59 6.1 結論 59 6.2 建議 60 參考文獻 61 附錄 66 附錄一 高通量定序使用之barcodes 66 | |
| dc.language.iso | zh-TW | |
| dc.subject | 銅 | zh_TW |
| dc.subject | 硫酸銅 | zh_TW |
| dc.subject | 底泥 | zh_TW |
| dc.subject | 微生物菌群分布 | zh_TW |
| dc.subject | 次世代定序 | zh_TW |
| dc.subject | sediments | en |
| dc.subject | copper sulfate | en |
| dc.subject | microbial community profile | en |
| dc.subject | next generation sequencing | en |
| dc.subject | copper | en |
| dc.title | 以模擬底泥反應槽之菌群分布作為鑑識銅汙染源指標 | zh_TW |
| dc.title | Copper Pollution Source Identification by Microbial Community Profiling of Sediment Microcosm | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林立虹(Li-Hung Lin),江殷儒(Yin-Ru Chiang),于昌平(Chang-Ping Yu) | |
| dc.subject.keyword | 銅,硫酸銅,底泥,微生物菌群分布,次世代定序, | zh_TW |
| dc.subject.keyword | copper,copper sulfate,sediments,microbial community profile,next generation sequencing, | en |
| dc.relation.page | 67 | |
| dc.identifier.doi | 10.6342/NTU202004236 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-10-08 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| Appears in Collections: | 環境工程學研究所 | |
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| U0001-0510202012403200.pdf Restricted Access | 4.62 MB | Adobe PDF |
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