請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72062
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 靳宗洛(Tsung-Luo Jinn) | |
dc.contributor.author | Lynne Stracovsky | en |
dc.contributor.author | 石凌 | zh_TW |
dc.date.accessioned | 2021-06-17T06:21:26Z | - |
dc.date.available | 2021-08-21 | |
dc.date.copyright | 2018-08-21 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-18 | |
dc.identifier.citation | Adams E, Diaz C, Matsui M, Shin R (2014) Overexpression of a novel component induces HAK5 and enhances growth in Arabidopsis. ISRN Botany 2014
Ahuja I, Vos RCHD, Bones AM, Hall RD (2010) Plant molecular stress responses face climate change. Trends Plant Sci 15: 664-674 Al-Whaibi MH (2011) Plant heat-shock proteins: a mini review. J. King Saud Univ. – Sci. 23: 139-150 Cao S, Jiang L, Song S, Jing R, Xu G (2006) AtGRP7 is involved in the regulation of abscisic acid and stress responses in arabidopsis. Cell Mol Biol Lett 11: 526-535 Chan Z (2012) Expression profiling of ABA pathway transcripts indicates crosstalk between abiotic and biotic stress responses in arabidopsis. Genomics 100: 110-115 Charng YY, Liu HC, Liu NY, Hsu FC, Ko SS (2006) Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol 140: 1297-1305 Chen AP, Zhong NQ, Qu ZL, Wang F, Liu N, Xia GX (2007) Root and vascular tissue-specific expression of glycine-rich protein AtGRP9 and its interaction with AtCAD5, a cinnamyl alcohol dehydrogenase, in Arabidopsis thaliana. J Plant Res 120: 337-343 Chen X, Zeng QC, Lu XP, Yu DQ, Li WZ (2010) Characterization and expression analysis of four glycine-rich RNA-binding proteins involved in osmotic response in tobacco (Nicotiana tabacum cv. Xanthi). Agr Sci China 9: 1577-1587 Clough SJ, Bent AF Vapor-phase sterilization of arabidopsis seed. University of Illinois at Urbana-Champaign. http://www.tonsorlab.pitt.edu/wp-content/uploads/2011/09/Seed-Sterilization.pdf Condit CM, McLean BG, Meagher RB (1990) Characterization of the expression of the petunia. Plant Physiol: 596-602 Czechowski T (2005) Genome-wide identification and testing of superior reference genes for transcript normalization in arabidopsis. Plant Physiol 139: 5-17 Czolpinska M, Rurek M (2018) Plant glycine-rich proteins in stress response: an emerging, still prospective story. Front Plant Sci 9: 1-13 De Oliveira DE, Franco LO, Simoens C, Seurinck J, Coppieters J, Botterman J, Vanmontagu M (1993) Inflorescence-specific genes from Arabidopsis-Thaliana encoding glycine-rich proteins. Plant J 3: 495-507 Finkelstein R (2013) Abscisic acid synthesis and response. Arabidopsis Book 11: e0166 Finkelstein RR, Gampala SSL, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14: S15-S45 Fusaro AF, Bocca SN, Ramos RLB, Barrôco RM, Magioli C, Jorge VC, Coutinho TC, Rangel-Lima CM, De Rycke R, Inzé D, Engler G, Sachetto-Martins G (2007) AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development. Planta 225: 1339-1351 Gomez J, Sánchez-Martínez D, Stiefel V, Rigau J, Puigdomènech P, Pagès M (1988) A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334: 262-264 Hanano S, Sugita M, Sugiura M (1996) Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells. Plant Mol Biol 31: 57-68 Hsu SF, Lai HC, Jinn TL (2010) Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis. Plant Physiol 153: 773-784 Huang YC, Niu CY, Yang CR, Jinn TL (2016) The heat stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiol 172: 1182-1199 Jakoby MJ, Falkenhan D, Mader MT, Brininstool G, Wischnitzki E, Platz N, Hudson A, Hulskamp M, Larkin J, Schnittger A (2008) Transcriptional profiling of mature Arabidopsis trichomes reveals that NOECK encodes the MIXTA-like transcriptional regulator MYB106. Plant Physiol 148: 1583-1602 Kazan K, Lyons R (2016) The link between flowering time and stress tolerance. J Exp Bot 67: 47-60 Kim JY, Park SJ, Jang B, Jung C-H, Ahn SJ, Goh C-H, Cho K, Han O, Kang H (2007) Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50: 439-451 Kotak S, Larkindale J, Lee U, von Koskull-Döring P, Vierling E, Scharf K-D (2007) Complexity of the heat stress response in plants. Curr Opin Plant Biol 10: 310-316 Krishna P (2003) 3 Plant responses to heat stress. Stress 4 Larkindale J, Hall D, Knight MR, Vierling E (2005) Heat stress phenotypes of arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol 138: 882-897 Larkindale J, Vierling E (2007) Core genome responses involved in acclimation to high temperature. Plant Physiol 146: 748-761 Le Gall H, Philippe F, Domon JM, Gillet F, Pelloux J, Rayon C (2015) Cell wall metabolism in response to abiotic stress. Plants 4: 112-166 Lee HJ, Ryu H, Chung KS, Posé D, Kim S, Schmid M, Ahn JH (2013) Regulation of temperature-responsive flowering by MADS-box transcription factor repressors. Science 342: 628-632 Li X (2011) Infiltration of Nicotiana benthamiana protocol for transient expression via Agrobacterium. Bio-Protocol 1 Lima RB, Dos Santos TB, Vieira LGE, Ferrarese MDLL, Ferrarese-Filho O, Donatti L, Boeger MRT, Petkowicz CLDO (2013) Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.). Carbohydr Polym 93: 135-143 Mangeon A, Junqueira RM, Sachetto-Martins G (2010) Functional diversity of the plant glycine-rich proteins superfamily. Plant Signaling Behav 5: 99-104 Mangeon A, Magioli C, Menezes-Salgueiro AD, Cardeal V, de Oliveira C, Galvao VC, Margis R, Engler G, Sachetto-Martins G (2009) AtGRP5, a vacuole-located glycine-rich protein involved in cell elongation. Planta 230: 253-265 Marty I, Monfort a, Stiefel V, Ludevid D, Delseny M, Puigdomènech P (1996) Molecular characterization of the gene coding for GPRP, a class of proteins rich in glycine and proline interacting with membranes in Arabidopsis thaliana. Plant Mol Biol 30: 625-636 Mundy J, Chua N-H (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 507-512 Nakashima K, Ito Y, Yamaguchi-Shinozaki K (2009) Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol 149: 88-95 Nover L, Bharti K, Döring P, Mishra SK, Ganguli A, Scharf K-D (2001) Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones 6: 177-177 Posé D, Verhage L, Ott F, Yant L, Mathieu J, Angenent GC, Immink RG, Schmid M (2013) Temperature-dependent regulation of flowering by antagonistic FLM variants. Nature 503: 414-417 Qin F, Shinozaki K, Yamaguchi-Shinozaki K (2011) Achievements and challenges in understanding plant abiotic stress responses and tolerance. Plant Cell Physiol 52: 1569-1582 Qu AL, Ding YF, Jiang Q, Zhu C (2013) Molecular mechanisms of the plant heat stress response. Biochem Biophys Res Commun 432: 203-207 Raghavendra AS, Gonugunta VK, Christmann A, Grill E (2010) ABA perception and signalling. Trends Plant Sci 15: 395-401 Ringli C, Keller B, Ryser U (2001) Glycine-rich proteins as structural components of plant cell walls. Cell Mol Life Sci 58: 1430-1441 Rodríguez-Hernández AA, Ortega-Amaro MA, Delgado-Sánchez P, Salinas J, Jiménez-Bremont JF (2014) AtGRDP1 gene encoding a glycine-rich domain protein is involved in germination and responds to ABA signalling. Plant Mol Biol Rep 32: 1187-1202 Ross JH, Murphy DJ (1996) Characterization of anther-expressed genes encoding a major class of extracellular oleosin-like proteins in the pollen coat of Brassicaceae. Plant J 9: 625-637 Ruiter RK, Van Eldik GJ, Van Herpen RM, Schrauwen JA, Wullems GJ (1997) Characterization of oleosins in the pollen coat of Brassica oleracea. Plant Cell 9: 1621-1631 Ryser U, Keller B (1992) Ultrastructural localization of a bean glycine-rich protein in unlignified primary walls of protoxylem cells. Plant Cell 4: 773-783 Sachetto-Martins G, Franco LO, de Oliveira DE (2000) Plant glycine-rich proteins: a family or just proteins with a common motif? Biochem Biophys 1492: 1-14 Showalter AM (1993) Structure and function of plant cell wall proteins. Plant Cell 5: 9-23 Sinha S, Raxwal VK, Joshi B, Jagannath A, Katiyar-Agarwal S, Goel S, Kumar A, Agarwal M (2015) De novo transcriptome profiling of cold-stressed siliques during pod filling stages in Indian mustard (Brassica juncea L.). Front Plant Sci 6: 932 Sorensen I, Domozych D, Willats WG (2010) How have plant cell walls evolved? Plant Physiol 153: 366-372 Takeno K (2016) Stress-induced flowering: the third category of flowering response. J Exp Bot 67: 4925-4934 Tuteja N (2007) Abscisic acid and abiotic stress signaling. Plant Signal Behav 2: 135-138 Ueki S, Citovsky V (2002) The systemic movement of a tobamovirus is inhibited by a cadmiumion-induced glycine-rich protein. Nat Cell Biol 4: 478-485 Umezawa T, Nakashima K, Miyakawa T, Kuromori T, Tanokura M, Shinozaki K, Yamaguchi-Shinozaki K (2010) Molecular basis of the core regulatory network in ABA responses: sensing, signaling and transport. Plant Cell Physiol 51: 1821-1839 Vierling E (1991) The roles of heat shock proteins in plants. Environment Xu K, Huang X, Wu M, Wang Y, Chang Y, Liu K, Zhang J, Zhang Y, Zhang F, Yi L, Li T, Wang R, Tan G, Li C (2014) A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis. PLoS One 9: e83556 Xu T, Gu L, Choi MJ, Kim RJ, Suh MC, Kang H (2014) Comparative functional analysis of wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding proteins in response to abiotic stresses. PLoS ONE 9: 1-8 Yang KA, Lim CJ, Hong JK, Park CY, Cheong YH, Chung WS, Lee KO, Lee SY, Cho MJ, Lim CO (2006) Identification of cell wall genes modified by a permissive high temperature in Chinese cabbage. Plant Sci 171: 175-182 Yoshida T, Mogami J, Yamaguchi-Shinozaki K (2014) ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Curr Opin Plant Biol 21: 133-139 Zhu Y, Zhu G, Guo Q, Zhu Z, Wang C, Liu Z (2013) A comparative proteomic analysis of Pinellia ternata leaves exposed to heat stress. Int J Mol Sci 14: 20614-20634 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72062 | - |
dc.description.abstract | 雖然富含甘胺酸蛋白質 (GRPs) 在近30年來已被分離鑑定出來,但我們對於其功能還不甚了解。根據先前的研究,已經知道GRPs分佈在細胞中不同位置且具有多樣的功能,以及其相關甘胺酸重複性的序列。阿拉伯芥中的富含甘胺酸蛋白AtGRP11及AtGRP12分別具有6.5%及26.5%的甘胺酸含量。為了近一步了解AtGRP11及AtGRP12的功能,我們對其進行功能及特性之分析。結果顯示,AtGRP11及AtGRP12皆位於細胞壁,而AtGRP11同時也分佈在細胞核中。利用-glucuronidase (GUS) 染色分析,發現AtGRP11會在植物維管束組織中的不同發育階段表現。相較於野生型,AtGRP11基因表現量降低的突變株具有根部較為短小的性狀,而AtGRP12基因的剃除突變株則有根部較長的性狀。此外,AtGRP11及AtGRP12基因皆會受到37℃的高溫誘導表現,而AtGRP11基因也會受到0℃的低溫及鹽逆境誘導表現。再者,分析各突變株的萌芽率及子葉綠化的情形,發現AtGRP11及AtGRP12基因會參與在 abscisic acid (ABA) 及鹽逆境的調控路徑當中,且AtGRP12基因更是參與在先天耐熱性的調控之中。本篇研究說明AtGRP11及AtGRP12基因各編碼著不同的細胞壁蛋白質,且皆會受到不同的非生物逆境誘導表現,可能在細胞壁結構的調控中扮演著重要的角色。 | zh_TW |
dc.description.abstract | Although glycine rich proteins (GRPs) in plants were isolated nearly 30 years ago, much remains unknown about their function. Previously studied GRPs have been found to have diverse localization and functions, as well as diverse quasi-repetitive glycine repeats. Arabidopsis glycine rich proteins AtGRP11 and AtGRP12 are characterized by 6.5% and 26.5% glycine content, respectively. We have functionally characterized these two proteins to better understand their roles. The results indicated that AtGRP11 and AtGRP12 are localized to the cell wall, and AtGRP11 is also localized to the nucleus. Tissue expression analysis revealed that AtGRP11 is expressed in the vascular tissue at various developmental stages of vegetative plant growth. AtGRP11-knockdown and AtGRP12-knockout mutant plants show shorter and longer root lengths, respectively, as compared to wild-type plants. Both AtGRP11 and AtGRP12 are induced by 37℃ heat, and AtGRP11 is also induced by 0℃ cold and salinity stresses. Moreover, analysis of germination rates and cotyledon greening in mutants indicate that AtGRP11 and AtGRP12 participate in abscisic acid (ABA) and salinity stress responses. Lastly, AtGRP12 was found to play a role in basal thermotolerance. This study shows that AtGRP11 and AtGRP12 are cell wall proteins that are induced by various abiotic stresses, and which possibly serve structural functions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:21:26Z (GMT). No. of bitstreams: 1 ntu-107-R04b42033-1.pdf: 2720040 bytes, checksum: 9a5482391c4eda07756bb110df37411b (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | ACKNOWLEDGEMENTS 2
摘 要 3 ABSTRACT 5 TABLE OF CONTENTS 7 LIST OF FIGURES 9 ABBREVIATIONS 11 INTRODUCTION 12 Heat Shock Response 13 Abscisic Acid Signaling 14 Characterization and Classification of Glycine-Rich Proteins 16 Subcellular Localization and Tissue Specific Expression of Glycine-Rich Proteins 18 Glycine-Rich Protein Response to Stresses 20 Motivation and Objectives 22 MATERIALS AND METHODS 24 Plant Materials and Growth Conditions 24 Constructs 24 ß-Glucuronidase (GUS) Activity Assay 25 RNA Extraction, cDNA synthesis, and Real-Time Quantitative PCR (qRT-PCR) 26 Protein Extraction and Immunoblotting Assay 26 Basal Thermotolerance Assay 27 Subcellular Localization of GRPs in Tobacco and Onion Epidermal Cells 27 Flowering Time and Expression of Flowering Genes 28 Root Length 28 Statistical Analysis 29 Primers and Accession Numbers 29 RESULTS 30 Arabidopsis AtGRP11 and AtGRP12 were Induced by Various Abiotic Stresses 30 Tissue Specific Expression Profiling of AtGRP11 32 Subcellular Localization of AtGRP11 and AtGRP12 33 Characterization of AtGRP11 and AtGRP12 T-DNA Insertion Mutants 33 AtGRP11 and AtGRP12 Mutant Phenotypes 34 AtGRP11 and AtGRP12 Mutant Response to ABA and Salinity Stress 35 AtGRP11 and AtGRP12 Mutant Response to Heat Stress 36 Characterization of AtGRP11 and AtGRP12 Overexpression Transgenic Plants 37 AtGRP11 and AtGRP12 Overexpression Mutant Response to Heat Stress 37 DISCUSSION 38 AtGRP11 and AtGRP12 Localize to the Cell Wall, Possibly Serving a Structural Function 40 AtGRP11 and AtGRP12 Play Important Roles in Abiotic Stress Tolerance 41 CONCLUSIONS AND FUTURE PROSPECTS 45 TABLES 47 FIGURES 49 SUPPLEMENTAL FIGURES 68 REFERENCES 72 APPENDIXES 77 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥兩個富含甘胺酸蛋白質在熱逆境及生長發育時期之功能性研究 | zh_TW |
dc.title | Functional Study of Two Glycine Rich Proteins under Abiotic Stress and during Development in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝旭亮(Hsu-Liang Hsieh),楊健志(Chien-Chih Yang),張英?(Ing-Feng Chang) | |
dc.subject.keyword | 富含甘胺酸蛋白,阿拉伯芥,開花時間,細胞壁,熱逆境, | zh_TW |
dc.subject.keyword | Arabidopsis,cell wall,flowering time,glycine rich proteins,heat stress,thermotolerance, | en |
dc.relation.page | 83 | |
dc.identifier.doi | 10.6342/NTU201803867 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 2.66 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。