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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 范士岡(Shih-Kang Fan) | |
| dc.contributor.author | Yu-Hsuan Yang | en |
| dc.contributor.author | 楊育璿 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:19:32Z | - |
| dc.date.available | 2023-08-21 | |
| dc.date.copyright | 2018-08-21 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-20 | |
| dc.identifier.citation | [1] M. Kovač, 'The bioinspiration design paradigm: A perspective for soft robotics,' Soft Robotics, vol. 1, pp. 28-37, 2014.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72022 | - |
| dc.description.abstract | 本研究利用電控微流體平臺跨尺度同時操控多種水膠與細胞之技術，配合事先設計好之電極圖案，以介電泳力將水膠溶液內C2C12骨骼肌纖維母細胞排列並使水膠交聯固化，以形成具特定三維細胞微結構之水膠微組件，並進行培養，探討不同三維微結構對於骨骼肌纖維母細胞生長與分化之影響。肌纖維母細胞在分化時會彼此融合並形成多核的肌小管，而每個肌小管為一個收縮單位，如何藉由肌纖維母細胞之排列使其在分化時能有等向性的肌小管形成，來達到最有效率之收縮力，為本研究致力之目標。本研究使用之水膠材料為Gelatin methacryloyl (GelMA)，加入光起始劑後成為一種光交聯性質之水膠，在照射UV光後會由液態轉為水膠態，此特性使得電控微流體技術得以在其為液態時運用電訊號操控之，並同時操控水膠內之細胞，並在曝光後產生內部具有固定細胞微結構之水膠微組件。在C2C12於GelMA水膠中進行分化後，可做為生物合成致動器之驅動微組件。以電控微流體技術，將含有細胞之驅動微組件以及其他水膠之結構微組件組合出任意的機構，甚至是改變基本微組件的大小來達到更為複雜的結構。我們已經成功建立出一基礎生物合成致動器，其為兩個結構微組件與一個致動微組件組成的1×3結構。經過12天的培養以及分化後，此基礎生物合成致動器可受到外接電訊號產生之一均勻電場刺激而產生收縮運動。我們進一步地將此致動器與Parylene材料進行封裝，期望改善細胞在分化時使致動器蜷曲的問題，在未來也可以進一步地將此致動器連接到不同的結構物上，以及進行可撓式微型電極的封裝，使其產生具時間性與空間性之驅動能力。 | zh_TW |
| dc.description.abstract | We constructed hydrogel microcomponets accommodating patterned skeletal muscle myoblasts (C2C12) by dielectrophoresis (DEP) force on an electromicrofluidic (EMF) platform due to its advanced manipulations of various hydrogels and cells simultaneously in cross-scale by electric signal application. The effects of aligned cell patterns on differentiation and myotubes maturation were analyzed. Myboblasts fuse and form multinucleated myotubes during differentiation, and each individual myotube is consider as a motor unit. To improve the contractility with highly aligned myotubes, the arrangement of myoblasts before differentiation is critical. By using photo-crosslinkable hydrogels, gelatin methacryloyl (GelMA), and pre-designed electrode patterns, hydrogel microcomponents with 3D cell microstructures were constructed after hydrogel polymerization. After C2C12 differentiation within GelMA, the microcomponents were used as driving microcomponents in a biohybrid-actuator. Furthermore, we assembled different driving microcomponents and structural hydrogel microcomponents to construct a biohybrid-actuator on an EMF platform. After 12 days in culture and differentiation, this biohybrid-actuator was actuated by electrical stimulation with a uniform electrical field around the actuator. With the proof-of-concept studies of the biohybrid-actuators, we further encapsulated the actuators with Parylene to improve the stability of the actuator during cell differentiation. In the future, the actuator would be attached on different solid surfaces and further integrated with flexible driving electrodes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:19:32Z (GMT). No. of bitstreams: 1 ntu-107-R05522109-1.pdf: 5794903 bytes, checksum: f8d056d97dffce8eae530fd141664fb8 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 目錄
致謝 i 中文摘要 ii Abstract iii 圖目錄 vii 表目錄 xiii 第1章 緒論 1 1-1 研究背景 1 1-2 文獻回顧 2 1-2.1 心肌組織建構之致動器 2 1-2.2 骨骼肌組織之建構 5 1-2.3 致動器之機構設計 10 1-2.4 外在訊號源之設計 12 1-3 研究動機 16 1-4 研究方法 19 第2章 電控微流體技術簡介 23 2-1 介電潤濕理論 23 2-2 液體介電泳理論 26 2-3 粒子介電泳理論 28 2-4 電控微流體平臺之技術與應用 29 第3章 實驗製程、儀器與系統介紹 31 3-1 電控微流體平臺製程 31 3-1.1 清洗玻璃基板 31 3-1.2 蒸鍍光阻貼附層 31 3-1.3 旋轉塗佈正光阻 31 3-1.4 曝光、顯影及定影 32 3-1.5 濕蝕刻 32 3-1.6 塗佈與沉積介電層 32 3-1.7 旋轉塗佈疏水層 33 3-1.8 上板表面處理 34 3-2 實驗系統 34 3-2.1 電控微流體平臺系統 34 3-2.2 細胞培養系統 35 3-2.3 水膠材料 39 3-2.4 曝光系統 42 3-2.5 電控微流體平臺 43 3-2.6 光罩圖案設計 43 3-2.7 細胞染色 45 第4章 實驗結果與討論 47 4-1 水膠與骨骼肌細胞於電控微流體平臺之操控與分析 47 4-1.1 建構不同三維細胞微結構之水膠微組件 47 4-1.2 水膠微組件之培養與免疫螢光染色分析 48 4-2 不同三維微結構對於骨骼肌細胞生長之影響與分析 51 4-2.1 建構特定條狀之三維細胞微結構之水膠微組件 51 4-2.2 不同條狀之三維細胞微結構之免疫螢光染色分析 53 4-3 生物合成致動器之初步設計與建構 55 4-3.1 生物合成致動器結構之材料測試 55 4-3.2 生物合成致動器之初步建構 57 4-3.3 以磁力固定生物合成致動器之建構 60 4-4 電訊號源之架設與致動器之驅動 62 4-4.1 基礎生物合成致動器之電訊號刺激分化與驅動分析 62 4-4.2 電訊號源之優化 66 4-5 生物合成致動器與Parylene結構之封裝 67 4-5.1 致動器之封裝設計討論 67 4-5.2 具2 'μ' m Parylene層之生物合成致動器之建構 68 4-5.3 具5 'μ' m Parylene層之生物合成致動器之建構 70 4-5.4 具Parylene層生物合成致動器之改進與實驗設計 72 第5章 結論與未來展望 74 5-1 結論 74 5-2 未來展望 74 參考文獻 77 | |
| dc.language.iso | zh-TW | |
| dc.subject | 三維細胞培養微結構 | zh_TW |
| dc.subject | 電控微流體 | zh_TW |
| dc.subject | C2C12分化 | zh_TW |
| dc.subject | 生物合成致動器 | zh_TW |
| dc.subject | 3D cell microstructure | en |
| dc.subject | C2C12 differentiation | en |
| dc.subject | biohybrid actuator | en |
| dc.subject | Electromicrofluidics (EMF) | en |
| dc.title | 以電控微流體平臺組成具三維骨骼肌細胞微結構之生物合成致動器 | zh_TW |
| dc.title | Construction of Biohybrid Actuators with 3D Skeletal Muscle Cell Microstructures on Electromicrofluidic Platform | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 范育睿(Yu-Jui Fan),許聿翔(Yu-Hsiang HSU) | |
| dc.subject.keyword | 電控微流體,三維細胞培養微結構,C2C12分化,生物合成致動器, | zh_TW |
| dc.subject.keyword | Electromicrofluidics (EMF),3D cell microstructure,C2C12 differentiation,biohybrid actuator, | en |
| dc.relation.page | 84 | |
| dc.identifier.doi | 10.6342/NTU201801212 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
| Appears in Collections: | 機械工程學系 | |
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|---|---|---|---|
| ntu-107-1.pdf Restricted Access | 5.66 MB | Adobe PDF |
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