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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李岳聯(yueh-lien Lee) | |
dc.contributor.author | Chung-Wen Hsu | en |
dc.contributor.author | 徐仲彣 | zh_TW |
dc.date.accessioned | 2021-06-17T06:16:28Z | - |
dc.date.available | 2023-09-03 | |
dc.date.copyright | 2018-09-03 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-30 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71955 | - |
dc.description.abstract | 本研究目的在尋找毒性較低且環保之殺菌劑,降低防污塗料毒性及防止環境重金屬汙染。藉由微生物特性分析硫酸鹽還原菌之生長曲線,並檢測殺菌劑之抑菌性,接著進行浸泡分析,使用光學顯微鏡、掃描式電子顯微鏡及共軛焦雷射掃描顯微鏡,觀察隨時間變化硫酸鹽還原菌之生物膜在金屬表面附著情形以及殺菌劑之效果,最後藉由電化學分析,由電化學數據判斷細菌對金屬底材造成的腐蝕及殺菌劑之抑菌性,最後使用光學顯微鏡和掃描式電子顯微鏡證實驗結果。
本研究第一部分為微生物特性分析,本實驗之菌種為硫酸鹽還原菌,量測並繪出其原始生長曲線,加入本研究選用之殺菌劑,十二烷基二甲基苄基氯化銨 (Benzyldimethyldodecyl ammonium Chloride),測量添加不同濃度下對硫酸鹽還原菌生長抑制的效果,藉此得出殺菌劑的最小抑制濃度(MIC,Minimum inhibitory concentration)。經濃度測試結果顯示,十二烷基二甲基苄基氯化銨具有抑制硫酸鹽還原菌生長的效果,其最小抑制濃度約為25ppm。 實驗第二部分為浸泡分析,由光學顯微鏡、掃描式電子顯微鏡及共軛焦雷射掃描顯微鏡觀察出接種硫酸鹽還原菌之金屬表面覆蓋一層生物膜,隨浸泡時間增加生物膜之厚度也增加許多,而添加殺菌劑之金屬表面活細胞明顯減少許多且無生物膜附著之情形。 實驗第三部分為電化學分析,由動電位極化曲線結果顯示,當硫酸鹽還原菌附著在底材表面隨著時間增長將會造成腐蝕電流密度大幅增加,而添加殺菌劑之腐蝕電流密度雖然也增加,但幅度相對低且相對緩慢,藉由光學顯微鏡及掃描式電子顯微鏡觀察出硫酸鹽還原菌確實會造成金屬底材孔蝕之現象,同樣地添加殺菌劑之金屬表面孔蝕之情形相對較緩和。 | zh_TW |
dc.description.abstract | The aim of this study was to reduce the toxicity of antifouling coatings and to prevent heavy metal pollution in the environment and finding a biocide which was less toxic and eco-friendly. Detecting the bactericidal activity of compound by microbial experimental, determined whether bacteria attached, biofilm formation and bactericidal activity by electrochemical experimental. Finally, Optical microsocope, SEM and CLSM were used to certifiy previous experiments.
The first part was microbial experiments, the experimental results verify the ability of inhibit the sulfate-reducing bacteria (SRB) growing of biocide, Benzyldimethyldodecyl ammonium chloride (DBMDAC), and detecting growth inhibition effect in different concentration, the minimal inhibitory concentration is 25ppm. The second part was immersion experiments, according to the Optical Microscope (OM), Scanning Electron Microscope (SEM) and Confocal Laser Scanning microscope (CLSM) results, there was no biofilm on the surface which added DBMDAC in the medium. The final part was electrochemical experiments, in dynamic potential polarization curve, attachment of bacteria causing current density increased greatly. The reason of this situation was pitting which was under the biofilm. However, the current density of test medium added BDMDAC increased slowly and the current density of sterile medium was stable. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:16:28Z (GMT). No. of bitstreams: 1 ntu-107-R05525021-1.pdf: 12662154 bytes, checksum: 8d7e5d563f64189a5274533fe9947768 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 致謝 i
摘要 ii 圖目錄 vii 表目錄 xii 第一章 前言 1 第二章 文獻回顧 3 2-1 腐蝕的定義與分類 3 2-1-1腐蝕的定義 3 2-1-2腐蝕的分類 3 2-2電化學腐蝕基本理論 10 2-2-1腐蝕熱力學 10 2-2-2腐蝕動力學 12 2-3海洋微生物腐蝕 15 2-3-1常見海洋汙損生物 15 2-3-2海洋微生物腐蝕機制 16 2-3-3 幾種典型引起腐蝕之微生物 16 2-4海洋微生物腐蝕的防護技術 20 2-4-1物理方法 20 2-4-2化學方法 20 2-4-4電化學檢測 22 第三章 實驗步驟及方法 26 3-1 實驗流程 26 3-2 金屬底材選用與製備 27 3-3 微生物特性分析 28 3-3-1 菌種培養 28 3-3-2 抗菌化合物選用 31 3-3-3 生長曲線及藥品毒性測試 32 3-4浸泡分析 34 3-4-1光學顯微鏡觀察與分析 34 3-4-2掃描式電子顯微鏡觀察與分析 35 3-4-3共軛焦雷射掃描顯微鏡觀察與分析 37 3-5電化學實驗方法 39 3-5-1恆電位儀 39 3-5-2動電位極化曲線 42 3-5-3動電位極化曲線後之表面形貌觀察 43 第四章 實驗結果與討論 44 4-1微生物特性分析之結果 44 4-2 浸泡分析之結果 49 4-2-1 光學顯微鏡觀察與分析 49 4-2-2 掃描式電子顯微鏡觀察與分析 59 4-2-3共軛焦雷射掃描顯微鏡觀察與分析 78 4-3 電化學分析之結果 92 4-3-1動電位極化曲線 92 4-3-2光學顯微鏡觀察與分析 95 4-3-3掃描式電子顯微鏡觀察與分析 102 第五章 結論 109 第六章 未來展望 111 參考文獻 112 | |
dc.language.iso | zh-TW | |
dc.title | 殺菌劑對304不鏽鋼表面生物膜成長及腐蝕行為之影響 –以硫酸鹽還原菌為例 | zh_TW |
dc.title | The use of Biocide to Control the Number and Activity of Sulfate Reducing Bacteria Biofilm and Corrosion Behaviors of 304 Stainless Steel | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 邱逢琛(Forng-Chen Chiu),簡順億(Shun-Yi Jian),羅凱尹(Kai-Yin Lo) | |
dc.subject.keyword | 海洋微生物,生物腐蝕,殺菌劑,電化學,動電位極化曲線, | zh_TW |
dc.subject.keyword | Marine Microorganisms,biological corrosion,biocides,electrochemical,dynamic potential polarization curves, | en |
dc.relation.page | 120 | |
dc.identifier.doi | 10.6342/NTU201804093 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 工程科學及海洋工程學研究所 | zh_TW |
顯示於系所單位: | 工程科學及海洋工程學系 |
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