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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王兆麟 | |
dc.contributor.author | Chun-Feng Lai | en |
dc.contributor.author | 賴淳風 | zh_TW |
dc.date.accessioned | 2021-06-17T06:16:17Z | - |
dc.date.available | 2018-09-07 | |
dc.date.copyright | 2018-09-07 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2018-09-05 | |
dc.identifier.citation | 1. Ker RF. Mechanics of tendon, from an engineering perspective. International Journal of Fatigue 2007;29:1001-9.
2. Korhonen RK, Saarakkala S. Biomechanics and Modeling of Skeletal Soft Tissues. In Klika V ed. Theoretical Biomechanics. Rijeka: InTech, 2011:Ch. 06. 3. Rowe RWD. The Structure of Rat Tail Tendon. Connective Tissue Research 1985;14:9-20. 4. Bruneau A, Champagne N, Cousineau-Pelletier P, et al. Preparation of rat tail tendons for biomechanical and mechanobiological studies. JoVE (Journal of Visualized Experiments) 2010:e2176-e. 5. Hansen KA, Weiss JA, Barton JK. Recruitment of tendon crimp with applied tensile strain. TRANSACTIONS-AMERICAN SOCIETY OF MECHANICAL ENGINEERS JOURNAL OF BIOMECHANICAL ENGINEERING 2002;124:72-7. 6. Rigby BJ, Hirai N, Spikes JD, et al. The Mechanical Properties of Rat Tail Tendon. The Journal of General Physiology 1959;43:265. 7. Haut R. Age-dependent influence of strain rate on the tensile failure of rat-tail tendon. J Biomech Eng 1983;105:296-9. 8. Kato YP, Christiansen DL, Hahn RA, et al. Mechanical properties of collagen fibres: a comparison of reconstituted and rat tail tendon fibres. Biomaterials 1989;10:38-42. 9. Dutov P, Antipova O, Varma S, et al. Measurement of Elastic Modulus of Collagen Type I Single Fiber. PloS one 2016;11:e0145711. 10. Wenger MPE, Bozec L, Horton MA, et al. Mechanical Properties of Collagen Fibrils. Biophysical Journal 2007;93:1255-63. 11. Svensson RB, Heinemeier KM, Couppé C, et al. Effect of aging and exercise on the tendon. Journal of Applied Physiology 2017;121:1237. 12. Lavagnino M, Gardner K, Arnoczky SP. Age-Related Changes in the Cellular, Mechanical, and Contractile Properties of Rat Tail Tendons. Connective Tissue Research 2013;54:70-5. 13. Li Y, Fessel G, Georgiadis M, et al. Advanced glycation end-products diminish tendon collagen fiber sliding. Matrix Biology 2013;32:169-77. 14. Goh KL, Holmes DF, Lu HY, et al. Ageing Changes in the Tensile Properties of Tendons: Influence of Collagen Fibril Volume Fraction. Journal of Biomechanical Engineering 2008;130:021011--8. 15. Haraldsson B, Aagaard P, Crafoord‐Larsen D, et al. Corticosteroid administration alters the mechanical properties of isolated collagen fascicles in rat‐tail tendon. Scandinavian journal of medicine & science in sports 2009;19:621-6.54 16. Haraldsson BT, Langberg H, Aagaard P, et al. Corticosteroids reduce the tensile strength of isolated collagen fascicles. The American journal of sports medicine 2006;34:1992-7. 17. Haraldsson BT, Aagaard P, Qvortrup K, et al. Lateral force transmission between human tendon fascicles. Matrix Biology 2008;27:86-95. 18. Szczesny SE, Caplan JL, Pedersen P, et al. Quantification of Interfibrillar Shear Stress in Aligned Soft Collagenous Tissues via Notch Tension Testing. Scientific Reports 2015;5:14649. 19. Kondratko-Mittnacht J, Duenwald-Kuehl S, Lakes R, et al. Shear Load Transfer in High and Low Stress Tendons. Journal of the mechanical behavior of biomedical materials 2015;45:109-20. 20. Hesjedal T, Behme G. The origin of ultrasound-induced friction reduction in microscopic mechanical contacts. IEEE transactions on ultrasonics, ferroelectrics, and frequency control 2002;49:356-64. 21. Teidelt E, Starcevic J, Popov VL. Influence of ultrasonic oscillation on static and sliding friction. Tribology Letters 2012;48:51-62. 22. Freundlich H, Gillings D. The influence of ultrasonic waves on the viscosity of colloidal solutions. Transactions of the Faraday Society 1938;34:649-60. 23. Gersten JW. Effect of ultrasound on tendon extensibility. American Journal of Physical Medicine & Rehabilitation 1955;34:362-9. 24. Morishita K, Karasuno H, Yokoi Y, et al. Effects of Therapeutic Ultrasound on Range of Motion and Stretch Pain. Journal of Physical Therapy Science 2014;26:711-5. 25. Iwashina T, Mochida J, Miyazaki T, et al. Low-intensity pulsed ultrasound stimulates cell proliferation and proteoglycan production in rabbit intervertebral disc cells cultured in alginate. Biomaterials 2006;27:354-61. 26. Edginton RS, Mattana S, Caponi S, et al. Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy. Journal of visualized experiments : JoVE 2016. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71951 | - |
dc.description.abstract | 目的:研究大鼠尾巴肌腱的機械性質與黏彈性,並探討大鼠尾巴肌腱在低強度超音波刺激下可能的改變。
背景簡介:肌腱的主要功能為連結與制動關節,連接在肌肉與骨骼之間。超音波對軟組織的熱效應已被廣泛應用於復健及治療之中,能夠舒緩疼痛並且提高關節自由度。但超音波如熱效應外,包括孔蝕(Cavitation)現象及表面振波等,都已被廣泛應用於工業界中,前者可以造成微結構的破壞,後者則在工業中應用於聲波潤滑。因此本研究希望能夠在降低超聲波熱能的影響下,對軟組織之機械性質與黏彈性做討論,希望能夠建立軟組織的微結構,包含膠原纖維之間的關聯等,在超音波照射下的所造成的改變。 材料與方法:(一)大鼠尾巴肌腱束抽取:大鼠在 12 周大時犧牲,本研究取得其尾巴作為實驗試樣。在小心地劃開大鼠尾巴皮膚及破壞椎體連接後,將肌腱束從大鼠尾巴遠端抽出。每條肌腱束被分為四段,一段長約 35 mm,每兩段被作為控制組與實驗組的成對試樣。每段皆透過光學顯微鏡照相,並量測截面積。實驗中另有三組鼠尾肌腱,透過藥物及酵素清洗取得純化的膠原纖維。(二)超音波系統設置:超音波系統由輸入端至輸出端,依序由波形產生器、RF 功率放大器、電功率計及其感測器、匹配電路到超音波探頭組成。超音波能量輸出透過聲功率計量測,在實驗環境中有 70 mW/cm2 的強度。(三)力學測試:力學測試以拉伸速率分為三組,依序分別為低應變速率組(0.01 mm/s)、中應變速率組(0.1 mm/s)及高應變速率組(0.8 mm/s)。在每一組不同的應變速率組中,又分別使用兩種不同的式樣,其中一種為鼠尾肌腱,另一組則為由鼠尾肌腱純化的膠原纖維。每組力學測試皆經過一固定 2%的應變大小,重複荷重測試五次,再執行拉斷測試。控制組與實驗組的不同,在於力學測試時超音波刺激的有無。 結果:(一)拉伸應變速率:控制組中,低應變速率的線性模數較其他兩組來得低,能量損失較其他兩組高,最大應力隨負荷所損失的比例,也是較其他兩組低。實驗組中有相同的趨勢,但不是每組皆有顯著差異。(二)面積影響:大鼠尾巴肌腱的隨面積增加而減少,從 200 MPa(面積大於 0.1 mm2),到 1200 MPa(面積小於 0.05 mm2)不等。藉由縮小截面積的範圍,各機械性質的標準差都可以被大幅降低(50%降至15%)。(三)超音波的影響:在所有的資料中,超音波刺激與控制組之間並沒有顯著差異,標準差過大。在縮小截面積所帶來的影響後,取截面積在 0.05 至 0.09 mm2之間(直徑為 300±25 um),會發現超音波在高應變速率組造成了顯著差異(p<0.05)。 (四)為節省實驗試樣,此研究中所使用的大鼠尾巴取自台大醫工所醫用陶瓷及複合材料實驗室,大鼠皆曾有過施打 OA 誘導高尿酸紀錄,然而在已知文獻回顧中,並不會影響肌腱力學性質。 結論:大鼠尾巴肌腱的力學性質,包括線性模數、最大應力以及能量損失等,對速率及面積的影響,已在本研究中完成。然而因為大鼠肌腱束的截面積變異,使模數與應力的標準差非常大。低能量超音波僅在高拉伸速率,也就是等同於生理負荷速度時,線性模數有顯著的降低。 | zh_TW |
dc.description.abstract | Objective: To study the mechanical and viscoelastic properties of the tendon, and to investigate the differences in those properties under low intensity ultrasound stimulation.
Summary of background data: The main function of the tendon is to connect and drive joints, and it lies between the muscle tissue and bone. Rat tail tendon grows parallel along with the tail, and are dispersed peripherally around vertebrate. Each tendon can be separated partly into 4 or 5 tendon fascicles. The effect of ultrasound, especially the thermal effect, has been well studied and widely used in therapeutics. Cavitation and surface acoustic waves have not been studied to the same extent as thermal effect despite their common appearance in industry. Cavitation can lead to micro structure damage and surface acoustic waves can reduce the friction force between two layers (acoustic lubricant) thus prompting this investigation. This study aims to investigate if the stimulation of low intensity ultrasound, which would not raise the temperature in tissue, would affect the mechanical and viscoelastic properties of tendon tissue. Methods: (1) Preparation of rat tail tendon: 12-week old rats were examined in this study. Rat tails were cut off from rats after they were sacrificed. After peeling off skin from tail, tendon fascicles were carefully pulled out from the distal end of the tail. Each tendon fascicle was equally divided into 4 parts. Two parts of the tendon fascicle were assigned to the intact group and the other 2 were assigned to experiment group. All of the tendon fascicles had pictures taken transversely in order to measuring their cross sectional area. Some of the rat tail tendons were immersed in enzymes to extract the pure extracellular matrix and collagen fibers from the tendon. (2) Setup of ultrasound system listed from the input to the output terminal: Ultrasound system was built with function generator, radio frequency amplifier, radio frequency power meter and its sensor, impedance matching circuit and ultrasound probe. The power of ultrasound was measured by the ultrasound acoustic meter, and then intensity was calculated after dividing power by the surface area of ultrasound probe. In this study, 70 mW/cm2 ultrasound intensity is delivered from the probe. (3) Mechanical testing protocol: There are three different groups depending on the cyclic loading rate, low strain rate (0.01 mm/s), middle strain rate (0.1 mm/s) and high strain rate (0.8 mm/s). In the high strain rate group, both rat tail tendon and purified collagen fibers bundles were examined. All the specimens underwent a 2% constant-strain cyclic load for 5 times. After cyclic loading, ramp to failure was conducted to determine the mechanical properties in linear region. The only difference between control group and experimental group is that there is an ultrasound stimulation during the loading. Result: (1) The average tangent modulus in the low strain rate group was higher than those in the mid strain rate group and the high strain rate group, but the average energy loss and max stress reduction rate is lower than those in mid strain rate group and high strain rate group. (2) The modulus of the rat tail tendon decreases while the cross section area increases. It ranges from 200 MPa (area greater than 0.1 mm2) to 1200 MPa (area less than 0.05 mm2). By grouping data points with smaller range of area, the relative standard deviation of modulus and max stress are reduced from 50% to 15%. (3) With all data points included, there is no significant difference between the intact group and experiment group. With the data points chosen with area in between 0.05 mm2 and 0.09 mm2, there is a significance in the high strain rate group between intact group and experiment group. Conclusion: The mechanical and viscoelastic properties of rat tail tendon were investigated. However, the variance of cross sectional area leads to a great standard deviation in modulus. Low intensity ultrasound only significantly reduces tangent modulus in high strain rate group when the variance of cross sectional area. Keywords: Rat tail tendon, mechanical properties, low-intensity ultrasound, therapeutic ultrasound | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:16:17Z (GMT). No. of bitstreams: 1 ntu-106-R04548014-1.pdf: 2470245 bytes, checksum: 8ae52fc884a4d623b0c6444e02ac65ab (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 誌謝 I
中文摘要 III Abstract V 圖目錄 XI 表目錄 XIII 第一章 緒論 1 1.1 肌腱構造與微結構 1 1.2 肌腱之力學研究 2 1.3 大鼠尾巴肌腱 3 1.3.1 大鼠尾巴肌腱機械性質 3 1.3.2 影響肌腱結構與機械性質的原因 4 1.3.3 剪切應力 5 1.4 超音波的簡介 5 1.4.1 超音波在工程的應用 6 1.4.2 超音波在醫療的應用 6 1.4.3 超音波與軟組織 7 1.5 實驗目的與假說 7 第二章 材料與方法 9 2.1 研究方法介紹 9 2.2 實驗程序 9 2.3 實驗儀器 11 2.3.1 拉伸測試機台(Bose Electroforce 5500) 11 2.3.2 電功率放大器(E&I 210L) 12 2.3.3 電功率計(Bird 4421) 與 感測器(Bird 4025) 12 2.3.4 超音波聲功率計(Ohmic UPM-DT-10PA) 13 2.3.5 超音波探頭 13 2.4 實驗流程 14 2.4.1 試樣準備 14 2.4.2 影像面積量測 15 2.4.3 超音波環境架設 16 2.4.4 力學測試 18 2.5 資料分析 20 第三章 實驗結果 21 3.1 超音波刺激系統 21 3.1.1 超音波匹配 21 3.1.2 超音波強度 21 3.2 面積量測結果 22 3.3 重複拉伸測試結果 24 3.3.1 能量損失 24 3.3.2 正切線模數 30 3.3.3 最大應力 36 第四章 討論 42 4.1 超音波能量與匹配 42 4.2 面積影像量測 42 4.3 肌腱力學性質 43 4.3.1 能量損失 43 4.3.2 最大線性模數 43 4.3.3 最大應力 48 4.3.4 純化ECM與一般肌腱束之比較 49 4.4 實驗限制 49 4.4.1 超音波強度量測 49 4.4.2 力學測試與夾製具 50 4.4.3 電訊號干擾 50 4.4.4 實驗試樣 51 第五章 結論與未來展望 52 5.1 結論 52 5.2 未來展望 52 參考文獻 53 | |
dc.language.iso | zh-TW | |
dc.title | 低強度超音波刺激對肌腱機械性質之影響 | zh_TW |
dc.title | Effect of Low Intensity Ultrasound Stimulation on
Mechanical Properties of Tendon Fascicle | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林文澧,趙本秀,劉浩澧,江惠華 | |
dc.subject.keyword | 大鼠尾巴肌腱,機械性質,低能量脈衝式超音波,超音波治療, | zh_TW |
dc.subject.keyword | Rat tail tendon,mechanical properties,low-intensity ultrasound,therapeutic ultrasound, | en |
dc.relation.page | 54 | |
dc.identifier.doi | 10.6342/NTU201804094 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-09-06 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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