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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71911完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 趙本秀(Pen-Hsiu Chao) | |
| dc.contributor.author | Chun-Wei Chang | en |
| dc.contributor.author | 張峻瑋 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:14:38Z | - |
| dc.date.available | 2023-10-17 | |
| dc.date.copyright | 2018-10-17 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-09-17 | |
| dc.identifier.citation | 1. A. M. Kiapour, R.F.a.M.M.M., Basic science of anterior cruciate ligament injury and repair. Bone Joint Res, 2014. 3(2): p. 20-31.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71911 | - |
| dc.description.abstract | 前十字韌帶受限於自身組織修復能力，因此組織工程也許可能提供足量新生組織。高分子緻密纖維材料雖具有良好力學強度，卻會限制細胞遷移以及胞外基質(ECM)沉積。本研究發現在緻密纖維材料上給予膠原蛋白塗層能幫助前十字韌帶細胞遷移進而讓細胞平均分散在整體材料中。我們篩選不同生長激素後發現纖維母細胞生長因子2型(FGF-2)能增加細胞數量，轉化生長因子-β1(TGF-β1) 能增加膠原蛋白生成，且次序性給予兩種不同生長激素比起單獨或混合給予能提高細胞數以及膠原蛋白的生成。最後我們利用血纖維蛋白凝膠將兩塊材料製備成複合物，不僅提高機械強度更能增進新生膠原蛋白的累積。未來我們會更進一步探討血纖維蛋白凝膠對生長因子的交互作用。 | zh_TW |
| dc.description.abstract | The limited repair ability of anterior cruciate ligament (ACL) prompted research in ligament tissue engineering to be a promising treatment strategy. We aim to optimize current issues of poor infiltration, poor ECM deposition and limited construct size. Collagen coating improved cell infiltration and resulted in homogeneous ligament cellularity on the densely fibrous PLLA scaffold. We found that the sequential growth factor supplementation of fibroblast growth factor-2 (FGF-2) and transforming growth factor beta-1 (TGF-β1) increased ACL cellularity and collagen deposition. To increase construct size, we laminated two layers of scaffolds with fibrin, which further improved collagen deposition and mechanical properties. Future study will focus on the interaction between fibrin gel and growth factors. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:14:38Z (GMT). No. of bitstreams: 1 ntu-107-R05548050-1.pdf: 4917698 bytes, checksum: de9b5397816764f389ae5998380b6ac2 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 中文摘要…………………………………………………………………………………………………………………………………iii
Abstract…………………………………………………………………………………………………………………………………iv Chapter 1 Introduction………………………………………………………………………………………………1 Chapter 2 Materials and Methods………………………………………………………………………5 2.1 Electrospun Scaffold…………………………………………………………………5 2.2 ECM Coating………………………………………………………………………………………………………………5 2.3 Cell culture……………………………………………………………………………………………………………6 2.4 Laminated scaffold……………………………………………………………………………………………7 2.5 Imaging ………………………………………………………………………………………………………………………7 2.6 Biochemical contents assay………………………………………………………………………8 2.7 RNA extraction and quantification of gene expression level……………………………………………………………………………………………………………………………………………9 2.8 Mechanical test…………………………………………………………………………………………………11 2.9 Statistical Analysis..………………………………………………………………………………11 Chapter 3 Results…………………………………………………………………………………………………………12 3.1 ECM coated PLLA scaffold for cell infiltration………………12 3.2 Growth factor supplement screening for ACL in vitro culture……………………………………………………………………………………………………………………………………16 3.3 Sequential growth factor supplementation………………………………18 3.4 Scaffold lamination………………………………………………………………………………………23 Chapter 4 Discussion…………………………………………………………………………………………………28 References……………………………………………………………………………………………………………………………32 | |
| dc.language.iso | en | |
| dc.subject | 膠原蛋白 | zh_TW |
| dc.subject | 纖維母細胞生長因子2型 | zh_TW |
| dc.subject | 轉化生長因子-β1 | zh_TW |
| dc.subject | 前十字韌帶細胞 | zh_TW |
| dc.subject | 血纖維蛋白 | zh_TW |
| dc.subject | Anterior cruciate ligament cell | en |
| dc.subject | basic fibroblast growth factor-2 | en |
| dc.subject | transforming growth factor beta-1 | en |
| dc.subject | fibrin | en |
| dc.title | 功能性韌帶組織工程最佳化:化學環境 | zh_TW |
| dc.title | Chemical Optimization for Functional Ligament Tissue Engineering | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡偉博(Wei-Bor Tsai),葉伊純(Yi-Cheun Yeh) | |
| dc.subject.keyword | 前十字韌帶細胞,膠原蛋白,纖維母細胞生長因子2型,轉化生長因子-β1,血纖維蛋白, | zh_TW |
| dc.subject.keyword | Anterior cruciate ligament cell,basic fibroblast growth factor-2,transforming growth factor beta-1,fibrin, | en |
| dc.relation.page | 36 | |
| dc.identifier.doi | 10.6342/NTU201804124 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-09-17 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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