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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 吳岳隆 | |
dc.contributor.author | Chia-Chi Tai | en |
dc.contributor.author | 戴家琪 | zh_TW |
dc.date.accessioned | 2021-06-17T06:09:37Z | - |
dc.date.available | 2020-12-20 | |
dc.date.copyright | 2018-12-20 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-11-30 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71782 | - |
dc.description.abstract | 桿狀病毒是可以感染多種無脊椎動物的環狀雙股DNA病毒,鱗翅目幼蟲是普遍最常見的寄主。其中,加州苜蓿夜蛾多角體病毒 (AcMNPV) 是最常被研究的桿狀病毒,於1980年代開始用於表現重組蛋白。家蠶核多角體病毒 (BmNPV) 是另一種也被廣泛運用於桿狀病毒表達載體系統 (BEVS) 以生產外源蛋白的病毒。這兩種核多角體病毒在序列同源性及基因結構上雖然具有高度相似性,但AcMNPV與BmNPV的寄主範圍卻完全沒有重疊。BmNPV在可容許 (permissive) 的Bm細胞株中能複製並產生病毒顆粒。然而,Bm細胞株對AcMNPV為不可容許(nonpermissive),因此並不會釋出新的病毒。已經有許多研究在探討關於決定這兩種桿狀病毒之間的寄主專一性的因素。例如,在哺乳動物中,細胞的選擇感受性、生理屏障或寄主防禦皆有可能導致特定組織的病毒感染。根據這個原因,我們推測寄主昆蟲也可能透過調節生理途徑影響病毒入侵。腺苷 (adenosine) 是一個作為能量調節信號的分子,在病原體入侵時提供能量誘導免疫反應。因此假設干擾腺苷的途徑或許能中斷能量並增加寄主感受性。目前我們的研究結果證實於家蠶細胞中抑制腺苷受體確實能提升原非允許病毒AcMNPV的感染能力,而家蠶蟲體實驗中則表現出相同的趨勢。了解病毒與寄主之間的專一性,可以為桿狀病毒在蛋白質表現系統的應用及生物製劑之開發帶來一個全新的突破。 | zh_TW |
dc.description.abstract | Baculoviruses are circular dsDNA viruses that infect many species of invertebrates, with lepidoptera larvae being the most common hosts. AcMNPV, the most commonly studied baculovirus, began to be used for expression of recombinant protein in 1980. BmNPV are another baculovirus widely used in BEVS to produce foreign proteins. These two NPVs are highly similar in sequence homology and genome organization, but the host ranges of AcMNPV and BmNPV are completely independent. BmNPVs replicate and produce viruses in the permissive Bm cell line. By contrast, AcMNPV is weakly expressed in the non-permissive Bm cell. There has been much research on the factors that determine host-specificity between these two baculoviruses. In mammals, for example, selective susceptibility of cells, physical barriers, or host defenses that potentially contribute to virus infection of specific tissues have been studied. For this reason, we theorize that insect hosts could possibly influence virus invasion through regulation of physiological pathways. Adenosine, a molecule that functions as an energy allocation signal, provides energy to induce immune systems when pathogens are infected. We can therefore assume that inhibition of adenosine pathways may interrupt energy flows and thus increase host. Our result confirm that inhibit the adenosine receptor in cells of silkworm can indeed enhance the infection ability of the original nonpermissive AcMNPV, while there are the same trend appeared in the experiment of silkworm larvae. The manipulation of virus-host specificity can provide a breakthrough for the application of baculovirus in protein expression systems and the development of bio-control agents. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:09:37Z (GMT). No. of bitstreams: 1 ntu-107-R05632010-1.pdf: 2321187 bytes, checksum: b0c5be92b24c1acfd53088de48468fef (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書 ii
中文摘要 iii Abstract iv 圖次 viii 表次 ix 壹、前言 1 貳、往昔研究 4 2.1 桿狀病毒 (Baculovirus) 4 2.1.1 桿狀病毒基本特徵與感染週期 4 2.1.2 加州苜蓿蛾核多角體病毒 (AcMNPV) 與家蠶核多角體病毒 (BmNPV) 4 2.1.3 影響寄主專一性的因素 5 2.2 家蠶 (Silk worm, Bombyx mori) 5 2.2.1生活史與家蠶飼養 5 2.2.2 歷史性、重要性及應用性 6 2.3 腺苷與其受體途徑 6 2.4碳水化合物 (carbohydrate) 代謝:糖解作用及檸檬酸循環 (TCA cycyle) 7 參、材料與方法 9 2.1 家蠶 (Bombyx mori)、細胞和病毒 9 2.2 siRNA之細胞轉染 (transfection) 與蟲體注射 (injection) 9 2.3 分離家蠶血淋巴 (hemolymph) 和脂肪體 (fatbody) 9 2.4葡萄糖分析 10 2.5海藻糖 (trehalose) 分析 10 2.6 核酸萃取 11 2.7 病毒核酸萃取 12 2.8即時定量聚合酶連鎖反應 12 2.9 統計分析 12 肆、結果 13 3.1 細胞實驗 (in vitro) 13 3.1.1 BmNPV與AcMNPV分別感染允許 (permissive) 及非允許 (nonpermissive) 細胞株 13 3.1.2 於Bm細胞中透過RNA干擾對腺苷受體進行基因抑制 14 3.1.3抑制adoR基因並感染AcMNPV 之酵素基因表現 15 3.2 家蠶蟲體實驗 (in vivo) 16 3.2.1 家蠶幼蟲感染BmNPV與AcMNPV之病毒表現 16 3.2.2 家蠶幼蟲感染BmNPV與AcMNPV之醣類代謝反應 16 伍、討論 18 陸、參考文獻 41 | |
dc.language.iso | zh-TW | |
dc.title | 抑制腺苷受體以增加寄主對桿狀病毒的感受性 | zh_TW |
dc.title | Inhibition of adenosine receptor increases host susceptibility to baculovirus infection | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 乃育昕,蔡志偉,吳佩穎 | |
dc.subject.keyword | 桿狀病毒,加州苜蓿夜蛾核多角體病毒,寄主專一性,腺?受體,能量, | zh_TW |
dc.subject.keyword | Baculovirus,AcMNPV,host specificity,adenosine receptor,energy, | en |
dc.relation.page | 45 | |
dc.identifier.doi | 10.6342/NTU201804276 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-12-03 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
顯示於系所單位: | 昆蟲學系 |
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