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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林致廷(Chih-Ting Lin) | |
| dc.contributor.author | Ting-Wei Lin | en |
| dc.contributor.author | 林庭瑋 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:08:49Z | - |
| dc.date.available | 2018-12-26 | |
| dc.date.copyright | 2018-12-26 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-12-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71758 | - |
| dc.description.abstract | 血液分離是生物醫學研究或應用中大量使用的樣品製備步驟之一。為了因應Point-Of-Care Technology (POCT)的趨勢，已有許多不同的微流道系統在設計或開發階段，用以實現在POCT的裝置中達到微量的血液分離功能。這些微流道設計開發的主要挑戰，在於微流道中處理微量的全血是一大困境。為了克服此困難，本研究提出了一種運用雕刻機製作的微流道設計，實現血漿提取功能的裝置。在此裝置的設計中結合了全血沉降槽以及協助分離的過濾膜。裝置中的幾何效應對於沉降效果以及過濾效率的影響皆為本研究中評估的項目，最終達到僅需15微升的全血樣本，並且於15分鐘完成沉降過濾，提取出無細胞(cell-free)的血漿樣本。電化學阻抗分析被用於驗證本研究血漿提取的設計，於30分鐘的生物分子反應鍵結後，即可進行量測。電化學阻抗分析可以進一步使此設計更接近符合POTC需求的診斷系統。基於本研究的設計所開發的血液分離裝置，可以被用於未來的個人保健相關應用。 | zh_TW |
| dc.description.abstract | Blood fractionation is an essential sample preparation step in in-vitro diagnosis applications. As a consequence, different microfluidic devices have been designed and developed to achieve blood fractionation functions, especially in Point-of-Care Technology (POCT). The major challenge of this development is difficulties to process small volume of whole blood in microscale devices. To conquer this challenge, we propose a plasma extraction device implemented by mechanically carving methods. In the proposed device, it combines a sedimentation slot and a filter membrane. The geometry effect of the sedimentaiton and filtration efficiency in the proposed device is experimentally evaluted. Only 15 microliter of whole blood sample is needed. Cell-free plasma sample can be extracted after 15 minutes of sedimentation and filtration. On the other hand, electrochemical impedance spectroscopy (EIS) is used to verify the plasma extract design. Further, it makes the device a step closer to a diagnosis system that should achieve POCT demand. 30 minutes is needed for hybridization, and the measurement can be conducted right after it. Based on our works, the developed blood-fractionation device can be used for future personal health care applications. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:08:49Z (GMT). No. of bitstreams: 1 ntu-107-R05945003-1.pdf: 3500438 bytes, checksum: 3c9d6ac7fb107c6557f664f82c915aff (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | Certification of approval by oral committee...............................................................i
Acknowledgment..........................................................................................................ii Abstract in Chinese.....................................................................................................iii Abstract in English......................................................................................................iv Contents.........................................................................................................................v List of Figures.............................................................................................................vii Chapter 1. Introduction...............................................................................................1 1.1 Structure of the Thesis.......................................................................................1 1.2 Overview of Cell Plasma Separation..................................................................2 1.3 Overview of Bio-molecular Sensing Technology.................................................7 1.4 Motivation of My Work...................................................................................10 Chapter 2. Theory.......................................................................................................12 2.1 Process of Blood Sedimentation.......................................................................12 2.2 Basis of Electrochemistry.................................................................................17 2.3 EIS and Equivalent Circuit Model...................................................................19 2.4 Measurement of EIS........................................................................................21 Chapter 3. Experiment...............................................................................................24 3.1 Device Design...................................................................................................24 3.2 Materials and Fabrication...............................................................................27 3.3 Experimental Setup and Process......................................................................30 3.4 EIS Measurement Parameters.........................................................................33 Chapter 4. Results and Discussion............................................................................35 4.1 Simulation of Sedimentation Slot.....................................................................35 4.1.1 Simulation Parameters......................................................................................35 4.1.2 Base Effect........................................................................................................37 4.1.3 Slope Effect......................................................................................................39 4.2 Validation of Sedimentation.............................................................................40 4.3 Validation of Filtration....................................................................................42 4.4 EIS Measurement............................................................................................45 4.4.1 Calibration Curve and Sensing Limitations......................................................45 4.4.2 Spiked Plasma and Diluted Plasma..................................................................46 4.4.3 Checkpoints: the Impedance of each Modification Step..................................48 4.5 Full Integration.............................................................................................50 Chapter 5. Conclusion................................................................................................51 References...................................................................................................................52 | |
| dc.language.iso | en | |
| dc.subject | 血液分離 | zh_TW |
| dc.subject | POTC | zh_TW |
| dc.subject | 血漿提取 | zh_TW |
| dc.subject | 血液沉降 | zh_TW |
| dc.subject | 過濾膜 | zh_TW |
| dc.subject | 電化學阻抗分析 | zh_TW |
| dc.subject | filter membranes | en |
| dc.subject | blood fractionation | en |
| dc.subject | POCT | en |
| dc.subject | electrochemical impedance spectroscopy (EIS) | en |
| dc.subject | plasma extraction | en |
| dc.subject | blood sedimentation | en |
| dc.title | 應用於個人化生物分子檢測之血液分離技術研發 | zh_TW |
| dc.title | A Design of Plasma Extraction for Bio-molecular Diagnosis in Personalized Applications | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃念祖(Nien-Tsu Huang),許聿翔(Yu-Hsiang Hsu) | |
| dc.subject.keyword | 血液分離,POTC,血漿提取,血液沉降,過濾膜,電化學阻抗分析, | zh_TW |
| dc.subject.keyword | blood fractionation,POCT,plasma extraction,blood sedimentation,filter membranes,electrochemical impedance spectroscopy (EIS), | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU201804357 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-12-19 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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