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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳信志(Shinn-Chih Wu) | |
dc.contributor.author | Yi-Chen Chen | en |
dc.contributor.author | 陳奕臣 | zh_TW |
dc.date.accessioned | 2021-06-17T06:08:29Z | - |
dc.date.available | 2021-12-21 | |
dc.date.copyright | 2018-12-21 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-12-20 | |
dc.identifier.citation | 1 Polge, C., Smith, A. U. & Parkes, A. S. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature 164, 666 (1949).
2 Blesbois, E., Grasseau, I. & Seigneurin, F. Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction 129, 371-378, doi:10.1530/rep.1.00454 (2005). 3 Gliozzi, T. M., Zaniboni, L. & Cerolini, S. DNA fragmentation in chicken spermatozoa during cryopreservation. Theriogenology 75, 1613-1622, doi:10.1016/j.theriogenology.2011.01.001 (2011). 4 Partyka, A., Nizanski, W. & Lukaszewicz, E. Evaluation of fresh and frozen-thawed fowl semen by flow cytometry. Theriogenology 74, 1019-1027, doi:10.1016/j.theriogenology.2010.04.032 (2010). 5 Saint Jalme, M., Lecoq, R., Seigneurin, F., Blesbois, E. & Plouzeau, E. Cryopreservation of semen from endangered pheasants: the first step towards a cryobank for endangered avian species. Theriogenology 59, 875-888 (2003). 6 Song, Y., Cheng, K. M., Robertson, M. C. & Silversides, F. G. Production of donor-derived offspring after ovarian transplantation between Muscovy (Cairina moschata) and Pekin (Anas platyrhynchos) ducks. Poultry Science 91, 197-200, doi:10.3382/ps.2011-01672 (2012). 7 Song, Y. & Silversides, F. G. Production of offspring from cryopreserved chicken testicular tissue. Poultry science 86, 1390-1396, doi:10.1093/ps/86.7.1390 (2007). 8 Trefil, P. et al. Restoration of spermatogenesis and male fertility by transplantation of dispersed testicular cells in the chicken. Biology of Reproduction 75, 575-581, doi:10.1095/biolreprod.105.050278 (2006). 9 Extavour, C. G. & Akam, M. Mechanisms of germ cell specification across the metazoans: epigenesis and preformation. Development 130, 5869-5884, doi:10.1242/dev.00804 (2003). 10 Tsunekawa, N., Naito, M., Sakai, Y., Nishida, T. & Noce, T. Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells. Development 127, 2741-2750 (2000). 11 Lavial, F. et al. Ectopic expression of Cvh (Chicken Vasa homologue) mediates the reprogramming of chicken embryonic stem cells to a germ cell fate. Developmental Biology 330, 73-82, doi:10.1016/j.ydbio.2009.03.012 (2009). 12 Lee, H. C. et al. DAZL expression explains origin and central formation of primordial germ cells in chickens. Stem Cells and Development 25, 68-79, doi:10.1089/scd.2015.0208 (2016). 13 Eyal-Giladi, H. & Kochav, S. From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. I. General morphology. Developmental Biology 49, 321-337 (1976). 14 Nakamura, Y. et al. Migration and proliferation of primordial germ cells in the early chicken embryo. Poultry Science 86, 2182-2193 (2007). 15 Hamburger, V. & Hamilton, H. L. A series of normal stages in the development of the chick embryo. Journal of Morphology 88, 49-92 (1951). 16 Kang, K. S. et al. Spatial and temporal action of chicken primordial germ cells during initial migration. Reproduction 149, 179-187, doi:10.1530/REP-14-0433 (2015). 17 Tajima, A. et al. Study on the concentration of circulating primordial germ cells (cPGCs) in early chick embryos. Journal of Experimental Zoology 284, 759-764 (1999). 18 Nakamura, Y., Kagami, H. & Tagami, T. Development, differentiation and manipulation of chicken germ cells. Development, Growth & Differentiation 55, 20-40, doi:10.1111/dgd.12026 (2013). 19 Fujimoto, T., Ukeshima, A. & Kiyofuji, R. The origin, migration and morphology of the primordial germ cells in the chick embryo. Anatomical Record 185, 139-145, doi:10.1002/ar.1091850203 (1976). 20 Stebler, J. et al. Primordial germ cell migration in the chick and mouse embryo: the role of the chemokine SDF-1/CXCL12. Developmental Biology 272, 351-361, doi:10.1016/j.ydbio.2004.05.009 (2004). 21 Ishimaru, Y. et al. Mechanism of asymmetric ovarian development in chick embryos. Development 135, 677-685, doi:10.1242/dev.012856 (2008). 22 Hughes, G. C. The population of germ cells in the developing female chick. Journal of Embryology and Experimental Morphology 11, 513-536 (1963). 23 Zheng, Y. H. et al. Expression pattern of meiosis associated SYCP family members during germline development in chickens. Reproduction 138, 483-492, doi:10.1530/REP-09-0163 (2009). 24 Smith, C. A., Roeszler, K. N., Bowles, J., Koopman, P. & Sinclair, A. H. Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid. BMC Developmental Biology 8, 85, doi:10.1186/1471-213X-8-85 (2008). 25 Yu, M. et al. RALDH2, the enzyme for retinoic acid synthesis, mediates meiosis initiation in germ cells of the female embryonic chickens. Amino Acids 44, 405-412, doi:10.1007/s00726-012-1343-6 (2013). 26 Kuramochi-Miyagawa, S. et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131, 839-849, doi:10.1242/dev.00973 (2004). 27 Vourekas, A. et al. Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis. Nature Structural & Molecular Biology 19, 773-781, doi:10.1038/nsmb.2347 (2012). 28 Kim, T. H. et al. Conserved functional characteristics of the PIWI family members in chicken germ cell lineage. Theriogenology 78, 1948-1959, doi:10.1016/j.theriogenology.2012.07.019 (2012). 29 Rengaraj, D. et al. Small non-coding RNA profiling and the role of piRNA pathway genes in the protection of chicken primordial germ cells. BMC Genomics 15, 757, doi:10.1186/1471-2164-15-757 (2014). 30 Siomi, M. C., Sato, K., Pezic, D. & Aravin, A. A. PIWI-interacting small RNAs: the vanguard of genome defence. Nature Reviews Molecular Cell Biology 12, 246-258, doi:10.1038/nrm3089 (2011). 31 Xu, L. et al. Piwil1 mediates meiosis during spermatogenesis in chicken. Animal Reproduction Science 166, 99-108, doi:10.1016/j.anireprosci.2016.01.008 (2016). 32 Chang, K. W. et al. Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development. BMC Genomics 19, 425, doi:10.1186/s12864-018-4820-9 (2018). 33 Macdonald, J., Glover, J. D., Taylor, L., Sang, H. M. & McGrew, M. J. Characterisation and germline transmission of cultured avian primordial germ cells. PLoS One 5, e15518, doi:10.1371/journal.pone.0015518 (2010). 34 van de Lavoir, M. C. et al. Germline transmission of genetically modified primordial germ cells. Nature 441, 766-769, doi:10.1038/nature04831 (2006). 35 Whyte, J. et al. FGF, insulin, and SMAD signaling cooperate for avian primordial germ cell self-renewal. Stem Cell Reports 5, 1171-1182, doi:10.1016/j.stemcr.2015.10.008 (2015). 36 Nakamura, Y. Poultry genetic resource conservation using primordial germ cells. Journal of Reproduction and Development 62, 431-437, doi:10.1262/jrd.2016-052 (2016). 37 Nandi, S. et al. Cryopreservation of specialized chicken lines using cultured primordial germ cells. Poultry Science 95, 1905-1911, doi:10.3382/ps/pew133 (2016). 38 Mizushima, S. et al. The birth of quail chicks after intracytoplasmic sperm injection. Development 141, 3799-3806, doi:10.1242/dev.111765 (2014). 39 Motono, M. et al. Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. Journal of Bioscience and Bioengineering 109, 315-321, doi:10.1016/j.jbiosc.2009.10.007 (2010). 40 Macdonald, J. et al. Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons. Proceedings of the National Academy of Sciences of the United States of America 109, E1466-1472, doi:10.1073/pnas.1118715109 (2012). 41 Park, T. S. & Han, J. Y. piggyBac transposition into primordial germ cells is an efficient tool for transgenesis in chickens. Proceedings of the National Academy of Sciences of the United States of America 109, 9337-9341, doi:10.1073/pnas.1203823109 (2012). 42 Olivier, S. et al. EB66 cell line, a duck embryonic stem cell-derived substrate for the industrial production of therapeutic monoclonal antibodies with enhanced ADCC activity. MAbs 2, 405-415 (2010). 43 Zhu, L. et al. Production of human monoclonal antibody in eggs of chimeric chickens. Nature Biotechnology 23, 1159-1169, doi:10.1038/nbt1132 (2005). 44 Raju, T. S., Briggs, J. B., Borge, S. M. & Jones, A. J. Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiology 10, 477-486 (2000). 45 Gilgunn, S. et al. Comprehensive N-Glycan Profiling of Avian Immunoglobulin Y. PLoS One 11, e0159859, doi:10.1371/journal.pone.0159859 (2016). 46 Schusser, B. et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proceedings of the National Academy of Sciences of the United States of America 110, 20170-20175, doi:10.1073/pnas.1317106110 (2013). 47 Park, T. S., Lee, H. J., Kim, K. H., Kim, J. S. & Han, J. Y. Targeted gene knockout in chickens mediated by TALENs. Proceedings of the National Academy of Sciences of the United States of America 111, 12716-12721, doi:10.1073/pnas.1410555111 (2014). 48 Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823, doi:10.1126/science.1231143 (2013). 49 Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826, doi:10.1126/science.1232033 (2013). 50 Dimitrov, L. et al. Germline gene editing in chickens by efficient CRISPR-mediated homologous recombination in primordial germ cells. PLoS One 11, e0154303, doi:10.1371/journal.pone.0154303 (2016). 51 Oishi, I., Yoshii, K., Miyahara, D., Kagami, H. & Tagami, T. Targeted mutagenesis in chicken using CRISPR/Cas9 system. Scientific Reports 6, 23980, doi:10.1038/srep23980 (2016). 52 Nakamura, Y. et al. Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos. Journal of Poultry Science 46, 127-135, doi:10.2141/Jpsa.46.127 (2009). 53 Song, Y., D'Costa, S., Pardue, S. L. & Petitte, J. N. Production of germline chimeric chickens following the administration of a busulfan emulsion. Molecular Reproduction and Development 70, 438-444, doi:10.1002/mrd.20218 (2005). 54 Nakamura, Y. et al. X-irradiation removes endogenous primordial germ cells (PGCs) and increases germline transmission of donor PGCs in chimeric chickens. The Journal of Reproduction and Development 58, 432-437 (2012). 55 Park, K. J. et al. Gamma-irradiation depletes endogenous germ cells and increases donor cell distribution in chimeric chickens. In vitro Cellular & Developmental Biology. Animal 46, 828-833, doi:10.1007/s11626-010-9361-8 (2010). 56 Taylor, L. et al. Efficient TALEN-mediated gene targeting of chicken primordial germ cells. Development 144, 928-934, doi:10.1242/dev.145367 (2017). 57 Liu, C. et al. Production of chicken progeny (Gallus gallus domesticus) from interspecies germline chimeric duck (Anas domesticus) by primordial germ cell transfer. Biology of Reproduction 86, 101, doi:10.1095/biolreprod.111.094409 (2012). 58 van de Lavoir, M. C. et al. Interspecific germline transmission of cultured primordial germ cells. PLoS One 7, e35664, doi:10.1371/journal.pone.0035664 (2012). 59 Marie-Etancelin, C. et al. Genetics and selection of mule ducks in France: a review. Worlds Poultry Science Journal 64, 187-207, doi:10.1017/S0043933907001791 (2008). 60 Maheshwari, S. & Barbash, D. A. The genetics of hybrid incompatibilities. Annual Review of Genetics 45, 331-355, doi:10.1146/annurev-genet-110410-132514 (2011). 61 Orr, H. A. Dobzhansky, Bateson, and the genetics of speciation. Genetics 144, 1331-1335 (1996). 62 Presgraves, D. C. The molecular evolutionary basis of species formation. Nature Reviews Genetics 11, 175-180, doi:10.1038/nrg2718 (2010). 63 Gregorova, S. & Forejt, J. PWD/Ph and PWK/Ph inbred mouse strains of Mus m. musculus subspecies--a valuable resource of phenotypic variations and genomic polymorphisms. Folia Biologica 46, 31-41 (2000). 64 Mihola, O., Trachtulec, Z., Vlcek, C., Schimenti, J. C. & Forejt, J. A mouse speciation gene encodes a meiotic histone H3 methyltransferase. Science 323, 373-375, doi:10.1126/science.1163601 (2009). 65 Oka, A., Mita, A., Takada, Y., Koseki, H. & Shiroishi, T. Reproductive isolation in hybrid mice due to spermatogenesis defects at three meiotic stages. Genetics 186, 339-351, doi:10.1534/genetics.110.118976 (2010). 66 Davies, B. et al. Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice. Nature 530, 171-176, doi:10.1038/nature16931 (2016). 67 Singhal, S. et al. Stable recombination hotspots in birds. Science 350, 928-932, doi:10.1126/science.aad0843 (2015). 68 Rigdon, R. H. & Mott, C. Testis in the sterile hybrid duck. A histologic and histochemical study. Veterinary Pathology 2, 553-565 (1965). 69 Islam, F. B. et al. Male hybrid sterility in the mule duck is associated with meiotic arrest in primary spermatocytes. Journal of Poultry Science 50, 311-320, doi:10.2141/Jpsa.0130011 (2013). 70 Snapir, N. et al. Testosterone concentrations, testes weight and morphology of mule drakes (Muscovy drake x Khaki Campbell). British Poultry Science 39, 572-574, doi:10.1080/00071669888791 (1998). 71 Brun, J., Richard, M., Marie-Etancelin, C., Rouvier, R. & Larzul, C. Le canard mulard: déterminisme génétique d'un hybride intergénérique. Productions Animales -Paris- Institut National de la Recherche Agronomique 18, 295 (2005). 72 Yamashina, Y. Studies on sterility in hybrid birds. V. Cytological study on the reciprocal hybrid between the Domestic duck and the Muscovy duck, with special reference to sterility and the valency of the sexual factor in the intergeneric hybrid. The Japanese Journal of Genetics 19, 209-218, doi:10.1266/jjg.19.209 (1943). 73 Lutz-Ostertag, Y. Le croisement canard Khaki-Campbell x Cane Barbarie. Archives d’Anatomie, d’Histologie et d’Embryologie Normales et Expérimentales 48, 300-327 (1965). 74 Denjean, B. et al. Caryotypes des canards commun (Anas platyrhynchos), Barbarie (Cairina moschata) et de leur hybride. Revue de Médecine Vétérinaire 148, 695-704 (1997). 75 Singh, H., Mok, P., Balakrishnan, T., Rahmat, S. N. & Zweigerdt, R. Up-scaling single cell-inoculated suspension culture of human embryonic stem cells. Stem Cell Research 4, 165-179, doi:10.1016/j.scr.2010.03.001 (2010). 76 Amit, M. et al. Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nature Protocols 6, 572-579, doi:10.1038/nprot.2011.325 (2011). 77 Otsuji, T. G. et al. A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production. Stem Cell Reports 2, 734-745, doi:10.1016/j.stemcr.2014.03.012 (2014). 78 Hiroki, O. et al. Effective transplantation of 2D and 3D cultured hepatocyte spheroids confirmed by quantum dot imaging. Advanced Biosystems 0, 1800137, doi:doi:10.1002/adbi.201800137 (2018). 79 Aihara, A., Abe, N., Saruhashi, K., Kanaki, T. & Nishino, T. Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions. Cancer Science 107, 1858-1866, doi:10.1111/cas.13095 (2016). 80 Higuchi, Y. et al. Functional polymer-dependent 3D culture accelerates the differentiation of HepaRG cells into mature hepatocytes. Hepatology Research 46, 1045-1057, doi:10.1111/hepr.12644 (2016). 81 Gupta, S. K. & Shukla, P. Sophisticated cloning, fermentation, and purification technologies for an enhanced therapeutic protein production: a Review. Frontiers in Pharmacology 8, 419, doi:10.3389/fphar.2017.00419 (2017). 82 Wells, E. & Robinson, A. S. Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement. Biotechnology Journal 12, doi:10.1002/biot.201600105 (2017). 83 Li, F., Vijayasankaran, N., Shen, A. Y., Kiss, R. & Amanullah, A. Cell culture processes for monoclonal antibody production. MAbs 2, 466-479 (2010). 84 Huang, Y. M. et al. Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment. Biotechnology Progress 26, 1400-1410, doi:10.1002/btpr.436 (2010). 85 Chu, L. & Robinson, D. K. Industrial choices for protein production by large-scale cell culture. Current Opinion in Biotechnology 12, 180-187 (2001). 86 Raju, T. S. Terminal sugars of Fc glycans influence antibody effector functions of IgGs. Current Opinion in Immunology 20, 471-478, doi:10.1016/j.coi.2008.06.007 (2008). 87 Shields, R. L. et al. Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. Journal Of Biological Chemistry 277, 26733-26740, doi:10.1074/jbc.M202069200 (2002). 88 Lillico, S. G. et al. Oviduct-specific expression of two therapeutic proteins in transgenic hens. Proceedings of the National Academy of Sciences of the United States of America 104, 1771-1776, doi:10.1073/pnas.0610401104 (2007). 89 Park, T. S. et al. Deposition of bioactive human epidermal growth factor in the egg white of transgenic hens using an oviduct-specific minisynthetic promoter. The FASEB Journal 29, 2386-2396, doi:10.1096/fj.14-264739 (2015). 90 Jung, J. G. et al. Characterization and application of oviductal epithelial cells in vitro in Gallus domesticus. Biology of Reproduction 85, 798-807, doi:10.1095/biolreprod.111.092023 (2011). 91 Stadnicka, K., Slawinska, A., Dunislawska, A., Pain, B. & Bednarczyk, M. Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica). BMC Developmental Biology 18, 9, doi:10.1186/s12861-018-0168-2 (2018). 92 Lohr, V. et al. New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation. Vaccine 27, 4975-4982, doi:10.1016/j.vaccine.2009.05.083 (2009). 93 Kraus, B. et al. Avian cell line - Technology for large scale vaccine production. BMC Proceedings 5 Suppl 8, P52, doi:10.1186/1753-6561-5-S8-P52 (2011). 94 Vautherot, J. F. et al. ESCDL-1, a new cell line derived from chicken embryonic stem cells, supports efficient replication of Mardiviruses. PLoS One 12, e0175259, doi:10.1371/journal.pone.0175259 (2017). 95 Leon, A. et al. The EB66(R) cell line as a valuable cell substrate for MVA-based vaccines production. Vaccine 34, 5878-5885, doi:10.1016/j.vaccine.2016.10.043 (2016). 96 Sebastian, S. & Gilbert, S. C. Recombinant modified vaccinia virus Ankara-based malaria vaccines. Expert Review of Vaccines 15, 91-103, doi:10.1586/14760584.2016.1106319 (2016). 97 Chandrasekaran, R. & Thailambal, V. G. The influence of calcium-ions, acetate and L-glycerate groups on the gellan double-helix. Carbohydrate Polymers 12, 431-442, doi:10.1016/0144-8617(90)90092-7 (1990). 98 Lei, Y. & Schaffer, D. V. A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation. Proceedings of the National Academy of Sciences of the United States of America 110, E5039-5048, doi:10.1073/pnas.1309408110 (2013). 99 Kim, Y. M. et al. The transgenic chicken derived anti-CD20 monoclonal antibodies exhibits greater anti-cancer therapeutic potential with enhanced Fc effector functions. Biomaterials 167, 58-68, doi:10.1016/j.biomaterials.2018.03.021 (2018). 100 Wu, H. et al. Purification and characterization of recombinant human lysozyme from eggs of transgenic chickens. PLoS One 10, e0146032, doi:10.1371/journal.pone.0146032 (2015). 101 Pain, B. et al. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development 122, 2339-2348 (1996). 102 Leighton, P. A., van de Lavoir, M. C., Diamond, J. H., Xia, C. & Etches, R. J. Genetic modification of primordial germ cells by gene trapping, gene targeting, and phiC31 integrase. Molecular Reproduction and Development 75, 1163-1175, doi:10.1002/mrd.20859 (2008). 103 Sorokin, L. M. & Morgan, E. H. Species specificity of transferrin binding, endocytosis and iron internalization by cultured chick myogenic cells. Journal of Comparative Physiology B 158, 559-566 (1988). 104 Guan, W. et al. Derivation and characteristics of pluripotent embryonic germ cells in duck. Poultry Science 89, 312-317, doi:10.3382/ps.2009-00413 (2010). 105 Chen, Y. C. et al. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS One 13, e0200515, doi:10.1371/journal.pone.0200515 (2018). 106 Raucci, F., Fuet, A. & Pain, B. In vitro generation and characterization of chicken long-term germ cells from different embryonic origins. Theriogenology 84, 732-742 e731-732, doi:10.1016/j.theriogenology.2015.04.032 (2015). 107 Tominaga, H. et al. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Analytical Communications 36, 47-50 (1999). 108 Fridolfsson, A. K. & Ellegren, H. A simple and universal method for molecular sexing of non-ratite birds. Journal of Avian Biology 30, 116-121, doi:10.2307/3677252 (1999). 109 Vallier, L., Alexander, M. & Pedersen, R. A. Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. Journal of Cell Science 118, 4495-4509, doi:10.1242/jcs.02553 (2005). 110 Park, T. S. & Han, J. Y. Derivation and characterization of pluripotent embryonic germ cells in chicken. Molecular Reproduction and Development 56, 475-482, doi:10.1002/1098-2795(200008)56:4<475::AID-MRD5>3.0.CO;2-M (2000). 111 Shamblott, M. J. et al. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proceedings of the National Academy of Sciences of the United States of America 95, 13726-13731 (1998). 112 Hayashi, K., Ohta, H., Kurimoto, K., Aramaki, S. & Saitou, M. Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. Cell 146, 519-532, doi:10.1016/j.cell.2011.06.052 (2011). 113 Jean, C. et al. Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC. Stem Cell Research 14, 54-67, doi:10.1016/j.scr.2014.11.005 (2015). 114 Cox, D. N., Chao, A. & Lin, H. piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells. Development 127, 503-514 (2000). 115 Ohinata, Y. et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436, 207-213, doi:10.1038/nature03813 (2005). 116 Yamaji, M. et al. Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Nature Genetics 40, 1016-1022, doi:10.1038/ng.186 (2008). 117 Naito, M. et al. Differentiation of donor primordial germ cells into functional gametes in the gonads of mixed-sex germline chimaeric chickens produced by transfer of primordial germ cells isolated from embryonic blood. Journal of Reproduction and Fertility 117, 291-298 (1999). 118 Chen, Y.-C. et al. Sperm quality parameters and reproductive efficiency in Muscovy duck (Cairina moschata). The Journal of Poultry Science 53, 223-232, doi:10.2141/ jpsa.0150162 (2016). 119 Jiang, F. et al. The complete mitochondrial genomes of the whistling duck (Dendrocygna javanica) and black swan (Cygnus atratus): dating evolutionary divergence in Galloanserae. Molecular Biology Reports 37, 3001-3015, doi:10.1007/s11033-009-9868-9 (2010). 120 Mott, C. L., Lockhart, L. H. & Rigdon, R. H. Chromosomes of the sterile hybrid duck. Cytogenetics 7, 403-412 (1968). 121 Blentic, A., Gale, E. & Maden, M. Retinoic acid signalling centres in the avian embryo identified by sites of expression of synthesising and catabolising enzymes. Developmental Dynamics 227, 114-127, doi:10.1002/dvdy.10292 (2003). 122 Chen, Y.-C. et al. In vitro culture and characterization of duck primordial germ cells. Poultry Science, pey515-pey515, doi:10.3382/ps/pey515 (2018). 123 Smith, E. A. Spermatogonial stem cell transfer to a mule Degree of Master of Science thesis, Middle Tennessee State University, (2015). 124 Kang, S. J. et al. Molecular and biological aspects of early germ cell development in interspecies hybrids between chickens and pheasants. Theriogenology 75, 696-706, doi:10.1016/j.theriogenology.2010.10.010 (2011). 125 Li, Z. D. et al. Production of duck-chicken chimeras by transferring early blastodermal cells. Poultry Science 81, 1360-1364, doi:10.1093/ps/81.9.1360 (2002). 126 Fuet, A. et al. NANOG is required for the long-term establishment of avian somatic reprogrammed cells. Stem Cell Reports 11, 1272-1286, doi:10.1016/j.stemcr.2018.09.005 (2018). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71747 | - |
dc.description.abstract | 始基生殖細胞為配子之前驅物,此等細胞可被視為種原保存之關鍵材料並被用於鳥類之遺傳多樣性管理。在始基生殖細胞移植後,為獲得配子及其子代,使用不具內源性生殖細胞之移植受體乃是理想之方式以提升被移植之始基生殖細胞之性腺傳承力。土番鴨乃公番鴨及母家鴨屬間雜交所得,此等雜交鴨雖具正常之體系發育,但配子發生時之缺陷乃致不育。因此,本試驗擬移植外源始基生殖細胞於土番鴨以探究其不育之成因及此鴨種成為生殖細胞移植通用受體之可能。
本研究之首要目的為分離並體外培養雞之始基生殖細胞,接著將此培養系統套用於培養不同鴨種之始基生殖細胞。首先,番鴨、北京鴨及雜交所得之土番鴨皆可被成功地分離出始基生殖細胞,細胞在體外增殖及遺傳修飾後其生殖系特性及發育潛力仍能維持。再者,始基生殖細胞被用以移植於土番鴨以進行配子發生之試驗。結果呈現,被始基生殖細胞移植之土番鴨無法產生卵或精子,但其精液中具有大量之細胞及細胞碎屑。此外,應用定量反轉錄聚合酶鏈鎖反應分析發現性腺體系中用來生成或降解維甲酸之酵素,其mRNA 表現量在發育過程之表現波動在親源鴨種及土番鴨中呈現非同步性。此等結果說明土番鴨性腺中之生殖細胞分化不全可能源於性腺發育時體細胞與生殖細胞間之交互作用。 本研究之次要目的為發展一套大量生產鳥禽類生殖細胞之方法。為此,試驗中建立了一種化學成分明確之培養液,可用以長期培養雞始基生殖細胞。此培養液結合功能性聚合物,發展為一種無需機械攪拌之三維懸浮培養系統以達到細胞量化生產之需求。量化生產之細胞易於冷凍保存,因此該系統可藉由擴增保存兩性別始基生殖細胞之遺傳資源進而貢獻於鳥類種原保存。此外,鳥禽類所展現之特殊醣基化修飾具醫療益處,成分明確之三維懸浮培養系統可做為雞始基生殖細胞之量化生產以達到生產重組蛋白及疫苗生產之目的。 | zh_TW |
dc.description.abstract | Primordial germ cells (PGCs) are the progenitors of gametes and could be seen as the key elements for germplasm preservation and the management of avian genetic biodiversity. To obtain the gametes and offspring, a recipient with no endogenous germ cells would be ideal to improve the germline transmission of the donor transplanted PGCs. In duck species, such bird exists as represented by the mule duck, produced by the intergeneric crossing between Muscovy drake (Cairina moschata) and female domestic duck (Anas platyrhynchos). This hybrid has normal somatic development but is impaired in the gamete production leading to hybrid sterility. Therefore, we aim to investigate the cause of this sterility and ultimately to test such breed as the universal recipient for transfer of exogenous duck PGCs to produce gametes of the donor genotype.
The first aim of the present project was to isolate and establish an in vitro culture of duck PGCs derived and adapted from the well-known chicken culture system. First, duck PGCs were successfully isolated from Muscovy, Pekin and hybrid mule ducks. Their germline characteristics and developmental potential were analyzed and were shown to be retained after being expanded in vitro and genetically modified. Second, those PGCs were transplanted to test the gametogenesis in mule duck but no sperm or ovum was produced in transplanted mule ducks. Instead, a large number of cells and debris was found in the semen collected from the mule drakes with PGC transplantation. Among others, mRNA levels of the gonadal somatic enzymes for retinoic acid (RA) synthesis and degradation showed asynchronous dynamic patterns in different developmental stages among parental species and mule ducks. Those observations could lead to the hypothesis of an impairment of the germ cell differentiation in the mule duck gonad that could be due to an altered germ-somatic cell-cell interaction leading to the sterility of the transplanted mule duck. The second aim of the project was to develop a process of large-scale amplification of avian germ cells. For that, a chemically defined medium was established to grow and amplify chicken PGCs in long-term culture. By adding a functional polymer in the medium, a three-dimensional (3D) suspension culture without mechanical stirring was established leading to a scalable production of PGCs. This system could also contribute to germplasm preservation by amplifying the genetic resource as PGCs of avian species in both genders. Those large-scale produced cells could thus be easily cryopreserved. Additionally, avian specific glycosylation has attracted therapeutic interests and the defined 3D suspension culture system could provide a useful platform for large-scale production of avian PGCs to produce recombinant proteins and vaccines. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:08:29Z (GMT). No. of bitstreams: 1 ntu-107-D02642001-1.pdf: 75792916 bytes, checksum: 39e650443482a99da886582b737e9983 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書 - #
ACKNEWLEGEMENTS - i 中文摘要 - ii ABSTRACT - iv RÉSUMÉ - vi CONTENTS - viii LIST OF FIGURES - xii LIST OF TABLES - xiv ABBREVISTIONS - xv Chapter 1 Literature review - 1 1.1 Germplasm preservation in birds - 1 1.2 The development of germ cells in avian - 2 1.3 Transplantation of primordial germ cells - 5 1.4 Duck industry in Taiwan and France - 7 1.5 Introduction of mule duck - 8 1.6 Specific aims - 12 Chapter 2 Three-dimensional culture of chicken primordial germ cells in defined media containing the functional polymer FP003 - 13 2.1 Abstract - 13 2.2 Introduction - 14 2.3 Materials and methods - 17 2.3.1 Incubation of chicken eggs - 17 2.3.2 Preparation of culture media - 17 2.3.3 Measurements of physical properties - 18 2.3.4 Establishment and in vitro culture of PGCs - 18 2.3.5 Cell proliferation assay - 19 2.3.6 Immunofluorescence and flow cytometric analysis - 20 2.3.7 RNA isolation and reverse transcription PCR (RT-PCR) - 20 2.3.8 Gonadal migration assay - 21 2.3.9 Statistical analysis - 21 2.4 Results - 22 2.4.1 Supplementation of FAcs medium with FP003 inhibits sedimentation - 22 2.4.2 Establishment of the optimal parameters for 3D culture of cPGCs - 24 2.4.3 Comparison of the growth of cPGCs between serum-containing and chemically defined media - 27 2.4.4 Expansion of cPGCs in 3D medium containing or lacking serum - 29 2.4.5 Maintenance of PGC characteristics upon long-term 3D culture - 31 2.4.6 Ectopic expression of recombinant fluorescent proteins in cPGC lines upon culture in 3D-FAot medium - 34 2.5 Discussion - 37 Chapter 3 In vitro culture and characterization of duck primordial germ cells - 41 3.1 Abstract - 41 3.2 Introduction - 41 3.3 Materials and methods - 43 3.3.1 Ducks and egg incubation - 43 3.3.2 Isolation and in vitro culture of PGCs - 44 3.3.3 Isolation and primary culture of embryonic fibroblasts - 45 3.3.4 Periodic Acid-Schiff staining - 45 3.3.5 Immunocytochemistry and flow cytometry - 45 3.3.6 RNA isolation and reverse transcription PCR (RT-PCR) - 46 3.3.7 Cell proliferation assay - 50 3.3.8 Lentiviral transduction and establishment of a transgenic chicken PGC line - 50 3.3.9 Gonadal migration assay - 51 3.3.10 Identification of species-specific genomic sequences following xenotransplantation - 51 3.3.11 Statistical analysis - 52 3.4 Results - 53 3.4.1 In vitro culture of Muscovy duck PGCs - 53 3.4.2 Comparison of MDgPGCs cultured in serum-containing and serum-free media - 57 3.4.3 The attempts of gPGC culture in chicken and ducks - 60 3.4.4 Immunocytochemical characterization of cultured chicken and duck gPGCs - 62 3.4.5 Other PGC related markers in chicken and duck gPGCs - 65 3.4.6 Gonadal migration of gPGCs after allogeneic and xenogeneic transplantation - 67 3.5 Discussion - 70 Chapter 4 Transplantation of primordial germ cells in sterile mule duck - 74 4.1 Abstract - 74 4.2 Introduction - 74 4.3 Materials and methods - 77 4.3.1 Duck and egg incubation - 77 4.3.2 Gonad tissue collection - 77 4.3.3 RNA isolation, RT-PCR and quantitative RT-PCR (qRT-PCR) - 78 4.3.4 Serum hormone detection - 79 4.3.5 Histological analysis of gonad tissue section - 79 4.3.6 Cell culture and transplantation - 80 4.3.7 Semen collection and sperm swim-up - 81 4.3.8 DNA isolation and identification of chicken genomic sequences - 82 4.3.9 Statistical analysis - 83 4.4 Results - 83 4.4.1 Analysis of gametogenesis related gene expression in three duck breeds - 83 4.4.2 Serum hormone levels in sex matured ducks - 90 4.4.3 Morphologies of reproductive organs and histological analysis of testis and ovary - 92 4.4.4 Production of germline chimera and their reproductive performance - 94 4.4.5 Track of donor PGCs in transplanted ducks - 103 4.5 Discussion - 106 CONCLUSION - 109 REFERENCE - 111 APPENDIX - 126 CURRICULUM VITAE - 140 RELEVANT PUBLICATIONS - 142 | |
dc.language.iso | en | |
dc.title | 建立以始基生殖細胞為媒介進行鳥禽保種之研究 | zh_TW |
dc.title | Establishment of avian biodiversity preservation platform via primordial germ cells (PGCs) | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 博士 | |
dc.contributor.coadvisor | 潘博通(Bertrand Pain) | |
dc.contributor.oralexamcommittee | 丁詩同(Shih-Torng Ding),陳銘正(Ming-Cheng Chen),劉秀洲(Hsiu-Chou Liu),林劭品(Shau-Ping Lin) | |
dc.subject.keyword | 種原保存,雞,鴨,始基生殖細胞,三維培養,雜交不育, | zh_TW |
dc.subject.keyword | germplasm preservation,chicken,duck,primordial germ cell (PGC),three-dimensional (3D) culture,hybrid sterility, | en |
dc.relation.page | 188 | |
dc.identifier.doi | 10.6342/NTU201804355 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-12-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
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