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標題: | 實用分析檢測裝置:製作與應用 Functional Analytical Devices: Fabrication and Application |
作者: | Chang-Ming Wang 王昌民 |
指導教授: | 廖尉斯 |
關鍵字: | 紙質微流道系統,封閉式紙質微流道系統,奈米水痕微影,電阻檢測裝置壓阻式海綿,介面, Paper-based microfluidics,enclosed paper-based analytical devices,meniscus,patterning,piezoresistive,pressure sensor,interfacing, |
出版年 : | 2019 |
學位: | 博士 |
摘要: | 此論文分為兩部分。第一部分探討封閉式紙質探測平台,包含封閉式紙質探測平台之簡介、極低螢光背景封閉式紙質探測平台之製作,以及可攜式雙極性電化學封閉式紙質探測平台之製作。紙質探測平台為近年來新興之分析裝置型式,利用常見的紙質材料作為製作分析裝置之基底,因其本身相較於傳統分析系統成本較低且易於取得,此種探測裝置在降低分析裝置之製作成本及操作成本上極具潛力。首先我們就封閉式紙質探測平台這一分支進行簡介,除了提供製作平台的基礎知識外,亦將眾多近年來社群所發表的文獻進行分類以利讀者了解。在創新研究方面,我們因觀察到市售護貝膠膜具有強烈的螢光性質,會在紫外光照射下放出藍色螢光,造成藍色螢光試劑無法與市售護貝膠模結合製作封閉式紙質探測裝置,是故我們自行以聚丙烯與聚乙烯醇縮丁醛兩種聚合物創造出一具極低螢光背景之護貝膠膜。我們以此種膠膜製作出具有極低藍色螢光背景的封閉式紙質探測裝置,可與發藍色螢光之試劑結合進行探測。再者,我們亦指出此種膠膜對於封裝其中之試劑有保護作用,可將酵素試劑之壽命自三天延長至至少七天。除了解決螢光背景之問題外,我們亦結合封閉式紙質裝置、雙極性電極與發光二極體製作出一低電壓需求之可攜式電化學探測裝置。此裝置係由護貝膠膜、銅膠帶,及發光二極體製作而成,可以市售之鈕扣電池作為電源以檢測具氧化還原活性之目標,如葡萄糖。我們預期藉由開發不同的封閉式紙質探測裝置可大幅降低分析化學應用於日常生活中的成本,並對於日常保健及環境探測等領域將有所貢獻。
此論文第二部分包含其他可應用於檢測裝置的創新技術。第四章中我們探討以聚乙烯奈米球為模板於聚合物表面製作出表面奈米結構,藉由將含有特定界面活性劑及聚乙烯奈米球之溶液置放於聚合物表面使其自然乾燥,並透過控制溶液之內容物及乾燥過程的環境便可得到如奈米環、奈米同心圓或奈米坑洞等不同的表面結構。首先在乾燥過程中將奈米球進行自組裝形成六方最密堆積結構,又因我們使用的界面活性劑可溶解表面之聚合物,被溶出的聚合物與界面活性劑將隨著乾燥的過程而一併堆積於奈米球模板下方,進而於表面形成各式不同之結構。我們深入探討各種結構的成因,並提出理論以解釋此過程。最後於第五章我們提出了一種可應用於海綿基底壓阻式壓力檢測裝置之介面設計,可以大幅增加該類壓力檢測裝置之靈敏度。海綿等多孔且可壓縮的材料常用於製作壓阻式壓力檢測裝置,其運作原理為觀察導電海綿基材受到壓力壓縮時的內部電阻變化以推算出施加壓力的大小。此類裝置多以銀漆接合海綿基材及電極,而我們提出以等間距之微米級銅導線取代銀漆作為介面材料,除可大幅增加施加壓力前後整個系統的電阻差外,於使用各種不同導電海綿基材時,所製得的壓阻式壓力檢測裝置之靈敏度也均可獲得大幅提升。 This dissertation contains two parts, the first part deals with enclosed paper-based devices, and the second part deals with novel techniques for device fabrication. Paper-based devices utilize common paper materials as the substrate, and aim at greatly reducing the analytical detection cost to benefit under-resourced communities, such as point-of-care scenarios or less developed regions. First, a brief history and introduction of enclosed paper-based devices are reviewed, and representative examples are given out. Commercially available lamination films made of polyethylene terephthalate and ethylene vinyl acetate, a common material for fabricating enclosed paper-based devices, exhibit a strong blue emission upon ultraviolet irradiation. Such property poses problems if blue fluorescent reagents are used in fabricating an enclosed paper-based device. We propose a novel composite polymer film made of polypropylene and polyvinyl butyral to overcome this concern. Results show that this film is clear of fluorescence under UV illumination, and improved detection limit and sensitivity are obtained when combining the lamination film with blue-fluorescent reagents. In addition, prolonged enzymatic reagent lifetime is observed when sealed with the composite film. In the second project, a portable enclosed paper-based electrochemical device is obtained by combining lamination techniques, bipolar electrode systems, and light-emitting diodes. The device is made with economic materials including lamination films, LEDs, and copper tapes, and is capable of detecting common targets such as hydrogen peroxide and glucose. Signal readout is accomplished by simply observing differences in LED brightness, and quantification can be achieved upon digital image acquisition. We envision that the development of low-cost and sturdy enclosed paper-based devices will greatly benefit their application in resource-limited settings. Chapter 4 of this dissertation focuses on the meniscus between nanospheres and a polymer surface, which is thought to be the cause of different surface feature formation after a spontaneous drying process. Parameters such as relative humidity and surfactant concentration of the solution are tuned to obtain different features, including nanorings, nanotargets, and nanoholes. These features arise from the surfactant-dissolved polymer accumulating beneath the templating nanospheres, while the locally-increased surfactant concentration also plays a major role. The underlying mechanism of this process is discussed and a theory for it is proposed. In the final chapter, a novel interface design for sponge-based piezoresistive pressure sensors is proposed. For this type of pressure sensors, a piece of conductive sponge is conventionally connected to the electrodes via silver paste. When an external pressure is applied to deform the sponge, the change in system resistance is recorded. We propose that in place of silver paste, ordered copper microwires can be used to greatly enhance the device sensitivity. This is due to the large difference in contact area at the interfacing region before and after the applied pressure. This sensitivity increase is also observed regardless of the sponge substrate type. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71565 |
DOI: | 10.6342/NTU201900209 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學系 |
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