請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71521
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張英? | |
dc.contributor.author | Wan-Ru Ying | en |
dc.contributor.author | 應婉如 | zh_TW |
dc.date.accessioned | 2021-06-17T06:02:24Z | - |
dc.date.available | 2024-02-14 | |
dc.date.copyright | 2019-02-14 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2019-01-30 | |
dc.identifier.citation | Adams, D.R., Ron, D., and Kiely, P.A. (2011). RACK1, a multifaceted scaffolding protein: structure and function. Cell Commun. Signal. 9,22.
Baccelli, I., Lombardi, L., Luti, S., Bernardi, R., Picciarelli, P., Scala, A., and Pazzagli, L. (2014). Cerato-platanin induces resistance in Arabidopsis leaves through stomatal perception, overexpression of salicylic acid and ethylene signalling genes and camalexin biosynthesis. PloS One 9, e100959. Benjamin, D., Colombi, M., Moroni, C., and Hall, M.N. (2011). Rapamycin passes the torch: a new generation of mTOR inhibitors. Nat Rev Drug Discov 10, 868-880. Berkowitz, O., Jost, R., Pollmann, S., and Masle, J. (2008). Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana. Plant Cell 20, 3430-3447. Boex-Fontvieille, E., Daventure, M., Jossier, M., Zivy, M., Hodges, M., and Tcherkez, G. (2013). Photosynthetic control of Arabidopsis leaf cytoplasmic translation initiation by protein phosphorylation. PloS One 8, e70692. Brandt, B., Brodsky, D.E., Xue, S.W., Negi, J., Iba, K., Kangasjarvi, J., Ghassemian, M., Stephan, A.B., Hu, H.H., and Schroeder, J.I. (2012). Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action. Proc. Natl. Acad. Sci. U. S. A. 109, 10593-10598. Chang, I.F., Szick-Miranda, K., Pan, S.Q., and Bailey-Serres, J. (2005). Proteomic characterization of evolutionarily conserved and variable proteins of. Arabidopsis cytosolic ribosomes. Plant Physiol. 137, 848-862. Chang, Y.Y. (2014) Functional study of receptor for activated C kinase 1 (RACK1) in drought stress response in Arabidopsis thaliana. National Taiwan University thesis. Chen, F., Li, Q., Sun, L., and He, Z. (2006a). The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Res. 13, 53-63. Chen, J.G., Ullah, H., Temple, B., Liang, J.S., Guo, J.J., Alonso, J.M., Ecker, J.R., and Jones, A.M. (2006b). RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis. J. Exp. Bot. 57, 2697-2708. Cheng, Z., Li, J.F., Niu, Y., Zhang, X.C., Woody, O.Z., Xiong, Y., Djonovic, S., Millet, Y., Bush, J., McConkey, B.J., Sheen, J., and Ausubel, F.M. (2015). Pathogen-secreted proteases activate a novel plant immune pathway. Nature 521, 213-216. Chinnusamy, V., Gong, Z., and Zhu, J.K. (2008). Abscisic acid-mediated epigenetic processes in plant development and stress responses. J. Integr. Plant Biol. 50, 1187-1195. Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735-743. De Smet, I., Signora, L., Beeckman, T., Inze, D., Foyer, C.H., and Zhang, H. (2003). An abscisic acid-sensitive checkpoint in lateral root development of Arabidopsis. Plant J. 33, 543-555. Deprost, D., Yao, L., Sormani, R., Moreau, M., Leterreux, G., Nicolai, M., Bedu, M., Robaglia, C., and Meyer, C. (2007). The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation. EMBO 8, 864-870. Dobrenel, T., Caldana, C., Hanson, J., Robaglia, C., Vincentz, M., Veit, B., and Meyer, C. (2016). TOR signaling and nutrient sensing. Annu. Rev. Plant Biol. 67, 261-285. Fennell, H., Olawin, A., Mizanur, R.M., Izumori, K., Chen, J.G., and Ullah, H. (2012). Arabidopsis scaffold protein RACK1A modulates rare sugar D-allose regulated gibberellin signaling. Plant Signal Behav. 7, 1407-1410. Finkelstein, R. (2013) Abscisic acid synthesis and response. Arabidopsis book 11, e0166 Fujita, Y., Fujita, M., Satoh, R., Maruyama, K., Parvez, M.M., Seki, M., Hiratsu, K., Ohme-Takagi, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2005). AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17, 3470-3488. Gonzalez, N., Vanhaeren, H., and Inze, D. (2012). Leaf size control: complex coordination of cell division and expansion. Trends Plant Sci. 17, 332-340. Gonzalez, N., De Bodt, S., Sulpice, R., Jikumaru, Y., Chae, E., Dhondt, S., Van Daele, T., De Milde, L., Weigel, D., Kamiya, Y., Stitt, M., Beemster, G.T., and Inze, D. (2010). Increased leaf size: different means to an end. Plant Physiol. 153, 1261-1279. Guo, J.J., and Chen, J.G. (2008). RACK1 genes regulate plant development with unequal genetic redundancy in Arabidopsis. BMC Plant Biol. 8, 108. Guo, J.J., Wang, J.B., Xi, L., Huang, W.D., Liang, J.S., and Chen, J.G. (2009). RACK1 is a negative regulator of ABA responses in Arabidopsis. J. Exp. Bot. 60, 3819-3833. Guo, J.J., Wang, S.C., Valerius, O., Hall, H., Zeng, Q.N., Li, J.F., Weston, D.J., Ellis, B.E., and Chen, J.G. (2011). Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid. Plant Physiol. 155, 370-383. Hoepflinger, M.C., Reitsamer, J., Geretschlaeger, A.M., Mehlmer, N., and Tenhaken, R. (2013). The effect of translationally controlled tumour protein (TCTP) on programmed cell death in plants. BMC Plant Biol. 13, 135. Hosy, E., Vavasseur, A., Mouline, K., Dreyer, I., Gaymard, F., Poree, F., Boucherez, J., Lebaudy, A., Bouchez, D., Very, A.A., Simonneau, T., Thibaud, J.B., and Sentenac, H. (2003). The Arabidopsis outward K+ channel GORK is involved in regulation of stomatal movements and plant transpiration. Proc. Natl. Acad. Sci. U. S. A. 100, 5549-5554. Ishida, S., Takahashi, Y., and Nagata, T. (1993). Isolation of cDNA of an auxin-regulated gene encoding a G protein beta subunit-like protein from tobacco BY-2 cells. Proc. Natl. Acad. Sci. U. S. A. 90, 11152-11156. Ishida, S., Takahashi, Y., and Nagata, T. (1996). The mode of expression and promoter analysis of the arcA gene, an auxin-regulated gene in tobacco BY-2 cells. Plant Cell Physiol. 37, 439-448. Islas-Flores, T., Rahman, A., Ullah, H., and Villanueva, M.A. (2015). The receptor for activated C kinase in plant signaling: tale of a promiscuous little molecule. Front Plant Sci. 6, 1090. Islas-Flores, T., Guillen, G., Alvarado-Affantranger, X., Lara-Flores, M., Sanchez, F., and Villanueva, M.A. (2011). PvRACK1 loss-of-function impairs cell expansion and morphogenesis in Phaseolus vulgaris L. root nodules. Mol. Plant 24, 819-826. Jha, S., Rollins, M.G., Fuchs, G., Procter, D.J., Hall, E.A., Cozzolino, K., Sarnow, P., Savas, J.N., and Walsh, D. (2017). Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase. Nature 546, 651-655. Kang, J., Hwang, J.U., Lee, M., Kim, Y.Y., Assmann, S.M., Martinoia, E., and Lee, Y. (2010). PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid. Proc. Natl. Acad. Sci. U. S. A. 107, 2355-2360. Kiely, P.A., Leahy, M., O'Gorman, D., and O'Connor, R. (2005). RACK1-mediated integration of adhesion and insulin-like growth factor I (IGF-I) signaling and cell migration are defective in cells expressing an IGF-I receptor mutated at tyrosines 1250 and 1251. J. Biol. Chem. 280, 7624-7633. Kim, Y.M., Han, Y.J., Hwang, O.J., Lee, S.S., Shin, A.Y., Kim, S.Y., and Kim, J.I. (2012). Overexpression of Arabidopsis translationally controlled tumor protein gene AtTCTP enhances drought tolerance with rapid ABA-induced stomatal closure. Mol. Cells. 33, 617-626. Kinoshita, T., and Hayashi, Y. (2011). New insights into the regulation of stomatal opening by blue light and plasma membrane H+-ATPase. Int. Rev. Cell Mol. Bio. 289, 89-115. Kundu, N., Dozier, U., Deslandes, L., Somssich, I.E., and Ullah, H. (2013). Arabidopsis scaffold protein RACK1A interacts with diverse environmental stress and photosynthesis related proteins. Plant Signal Behav. 8, e24012. Lin, C.H. (2011) RACK1A has no direct protein-protein interaction with 14-3-3 protein in Arabidopsis thaliana. National Taiwan University thesis. Li, S., Assmann, S.M., and Albert, R. (2006). Predicting essential components of signal transduction networks: a dynamic model of guard cell abscisic acid signaling. PLoS Biol. 4, e312. Liu, S.S., Li, H., Lv, X.Z., Ahammed, G.J., Xia, X.J., Zhou, J., Shi, K., Asami, T., Yu, J.Q., and Zhou, Y.H. (2016). Grafting cucumber onto luffa improves drought tolerance by increasing ABA biosynthesis and sensitivity. Sci. Rep. 6, 20212. Luo, X.J., Chen, Z.Z., Gao, J.P., and Gong, Z.Z. (2014). Abscisic acid inhibits root growth in Arabidopsis through ethylene biosynthesis. Plant J. 79, 44-55. Mahajan, S., and Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Arch Biochem Biophys. 444, 139-158. Meinhard, M., Rodriguez, P.L., and Grill, E. (2002). The sensitivity of ABI2 to hydrogen peroxide links the abscisic acid-response regulator to redox signaling. Planta 214, 775-782. Merlot, S., Gosti, F., Guerrier, D., Vavasseur, A., and Giraudat, J. (2001). The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signaling pathway. Plant J. 25, 295-303. Mochly-Rosen, D., Khaner, H., and Lopez, J. (1991). Identification of intracellular receptor proteins for activated protein kinase C. Proc. Natl. Acad. Sci. U. S. A. 88, 3997-4000. Montane, M.H., and Menand, B. (2013). ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change. J. Exp. Bot. 64, 4361-4374. Mori, I.C., and Murata, Y. (2011). ABA signaling in stomatal guard cells: lessons from Commelina and Vicia. J. Plant Res. 124, 477-487. Munemasa, S., Hauser, F., Park, J., Waadt, R., Brandt, B., and Schroeder, J.I. (2015). Mechanisms of abscisic acid-mediated control of stomatal aperture. Curr. Opin. Plant Biol. 28, 154-162. Pei, Z.M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G.J., Grill, E., and Schroeder, J.I. (2000). Calcium channels activated by hydrogen peroxide mediate abscisic acid signaling in guard cells. Nature 406, 731-734. Polak, P., and Hall, M.N. (2009). mTOR and the control of whole body metabolism. Curr. Opin. Cell Biol. 21, 209-218. Powell, A.E., and Lenhard, M. (2012). Control of organ size in plants. Curr. Biol. 22, R360-R367. Ren, M., Venglat, P., Qiu, S., Feng, L., Cao, Y., Wang, E., Xiang, D., Wang, J., Alexander, D., Chalivendra, S., Logan, D., Mattoo, A., Selvaraj, G., and Datla, R. (2012). Target of rapamycin signaling regulates metabolism, growth, and life span in Arabidopsis. Plant Cell 24, 4850-4874. Ron, D., Chen, C.H., Caldwell, J., Jamieson, L., Orr, E., and Mochlyrosen, D. (1994). Cloning of an intracellular receptor for protein kinase C- a homolog of the beta-subunit of G-proteins. Proc. Natl. Acad. Sci. U. S. A. 91, 839-843. Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2006). Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc. Natl. Acad. Sci. U. S. A. 103,18822-18827. Sehnke, P.C., DeLille, J.M., and Ferl, R.J. (2002). Consummating signal transduction: the role of 14-3-3 proteins in the completion of signal-induced transitions in protein activity. Plant Cell 14, S339-354. Sengupta, J., Nilsson, J., Gursky, R., Spahn, C.M.T., Nissen, P., and Frank, J. (2004). Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM. Nat. Struct. Mol. Biol. 11, 957-962. Sirichandra, C., Wasilewska, A., Vlad, F., Valon, C., and Leung, J. (2009). The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action. J. Exp. Bot. 60, 1439-1463. Sormani, R., Yao, L., Menand, B., Ennar, N., Lecampion, C., Meyer, C., and Robaglia, C. (2007). Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility. BMC Plant Biol. 7, 26. Speth, C., Willing, E.M., Rausch, S., Schneeberger, K., and Laubinger, S. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. 76, 433-445. Tian, G., Lu, Q., Zhang, L., Kohalmi, S.E., and Cui, Y.H. (2011). Detection of protein interactions in plant using a gateway compatible bimolecular fluorescence complementation (BiFC) System. J. Vis. Exp. 16, 55. Tian, L., DellaPenna, D., and Zeevaart, J.A.D. (2004). Effect of hydroxylated carotenoid deficiency on ABA accumulation in Arabidopsis. Physiol. Plantarum 122, 314-320. Toscano-Morales, R., Xoconostle-Cazares, B., Cabrera-Ponce, J.L., Hinojosa-Moya, J., Ruiz-Salas, J.L., Galvan-Gordillo, S.V., Guevara-Gonzalez, R.G., and Ruiz-Medrano, R. (2015). AtTCTP2, an Arabidopsis thaliana homolog of translationally controlled tumor protein, enhances in vitro plant regeneration. Front. Plant Sci. 6, 468. Tuteja, N. (2007). Abscisic acid and abiotic stress signaling. Plant Signal Behav.2, 135-138. Ullah, H., Scappini, E.L., Moon, A.F., Williams, L.V., Armstrong, D.L., and Pedersen, L.C. (2008). Structure of a signal transduction regulator, RACK1, from Arabidopsis thaliana. Protein Sci. 17, 1771-1780. Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T., and Shinozaki, K. (2009). Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proc. Natl. Acad. Sci. U. S. A. 106, 17588-17593. Urano, D., Czarnecki, O., Wang, X., Jones, A.M., and Chen, J.G. (2015). Arabidopsis receptor of activated C kinase1 phosphorylation by WITH NO LYSINE8 KINASE. Plant Physiol. 167, 507-516. Vahisalu, T., Kollist, H., Wang, Y.F., Nishimura, N., Chan, W.Y., Valerio, G., Lamminmaki, A., Brosche, M., Moldau, H., Desikan, R., Schroeder, J.I., and Kangasjarvi, J. (2008). SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling. Nature 452, 487-491. Villafuerte, B.C., Barati, M.T., Rane, M.J., Isaacs, S., Li, M., Wilkey, D.W., and Merchant, M.L. (2017). Over-expression of insulin-response element binding protein-1 (IRE-BP1) in mouse pancreatic islets increases expression of RACK1 and TCTP: Beta cell markers of high glucose sensitivity. Proteomics and Bioinformatics Analyses-Proteins Proteome. 1865, 186-194. Vincent, D., Ergul, A., Bohlman, M.C., Tattersall, E.A.R., Tillett, R.L., Wheatley, M.D., Woolsey, R., Quilici, D.R., Joets, J., Schlauch, K., Schooley, D.A., Cushman, J.C., and Cramer, G.R. (2007). Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity. J. Exp. Bot. 58, 1873-1892. Vishwakarma, K., Upadhyay, N., Kumar, N., Yadav, G., Singh, J., Mishra, R.K., Kumar, V., Verma, R., Upadhyay, R.G., Pandey, M., and Sharma, S. (2017). Abscisic acid signaling and abiotic stress tolerance in plants: a review on current knowledge and future prospects. Front. Plant Sci. 8, 161. Wani, S.H., Kumar, V., Shriram, V., and Sah, S.K. (2016). Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants. Crop J. 4, 162-176. Weiner, J.J., Peterson, F.C., Volkman, B.F., and Cutler, S.R. (2010). Structural and functional insights into core ABA signaling. Curr. Opin. Plant Biol. 13, 495-502. Wolters, H., and Jurgens, G. (2009). Survival of the flexible: hormonal growth control and adaptation in plant development. Nat. Rev. Genet. 10, 305-317. Xiong, F.J., Zhang, R., Meng, Z.G., Deng, K.X., Que, Y.M., Zhuo, F.P., Feng, L., Guo, S.D., Datla, R., and Ren, M.Z. (2017). Brassinosteriod Insensitive 2 (BIN2) acts as a downstream effector of the Target of Rapamycin (TOR) signaling pathway to regulate photoautotrophic growth in Arabidopsis. New Phytol. 213, 233-249. Yoo, S.D., Cho, Y.H., and Sheen, J. (2007). Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocol 2, 1565-1572. Yoshida, T., Mogami, J., and Yamaguchi-Shinozaki, K. (2014). ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Curr. Opin. Plant Biol. 21, 133-139. Zhang, D.P., Chen, L., Li, D.H., Lv, B., Chen, Y., Chen, J.G., Yan, X.J., and Liang, J.S. (2014). OsRACK1 is involved in abscisic acid- and H2O2-mediated signaling to regulate seed germination in rice (Oryza sativa, L.). PloS One 9, e97120. Zhang, D.P., Wang, Y.Z., Shen, J.Y., Yin, J.F., Li, D.H., Gao, Y., Xu, W.F., and Liang, J.S. (2018). OsRACK1A, encodes a circadian clock-regulated WD40 protein, negatively affect salt tolerance in rice. Rice 11, 45. Zhao, J., Gao, Y., Zhang, Z., Chen, T., Guo, W., and Zhang, T. (2013). A receptor-like kinase gene (GbRLK) from Gossypium barbadense enhances salinity and drought-stress tolerance in Arabidopsis. BMC Plant Biol.13, 110. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71521 | - |
dc.description.abstract | Receptor for activated C kinase 1 (RACK1) 是一個普遍存在於真核生物內,且具有高度保留性的基因。在阿拉伯芥中有三個RACK1的基因,包括RACK1A (At1g18080)、RACK1B (At1g48630) 及RACK1C (At3g18130),其中RACK1A扮演一個顯著表現角色。阿拉伯芥中的RACK1 蛋白質被視為鷹架蛋白,會與其他百種蛋白質交互作用並調節不同生理功能,AtRACK1A曾被報導為離層酸路徑的負向調節者。根據實驗室前人研究,發現雙子葉植物內RACK1蛋白質序列相較於其他物種甚至於單子葉植物多出大約十個胺基酸,然而這十個胺基酸序列對於雙子葉植物RACK1是否具有功能,以及為何不存在於其他物種,皆是尚未清楚。本研究比較RACK1A去除這十個胺基酸序列的大量表現轉殖植物(命名為XRA)、rack1a突變株與AtRACK1A大量表現株,在處理離層酸後觀察離層酸標的基因表現量以及性狀比較,發現離層酸處理後主根長縮短幅度以及氣孔開閉程度,XRA轉殖株皆與rack1a有較相似的性狀,顯示RACK1A特定出現於雙子葉植物的十個胺基酸片段可能參與調節離層酸訊息傳導路徑。另一方面,前人發現阿拉伯芥Translationally Controlled Tumor Protein 1 (AtTCTP1)與RACK1A有交互作用,而TCTP1為Target of Rapamycin (TOR)訊息傳導路徑ㄧ員,協助調節植物生長發育。本研究進一步發現rack1a突變株與XRA以及RACK1A-S286A點突變大量表現株在葉片生長發育相較WT有較小之趨勢,且處理TOR訊息傳導路徑的抑制劑AZD8055,觀察植物根部生長,發現rack1a突變株對於藥劑的
敏感度較低,暗示RACK1A可能參與在TOR路徑。 | zh_TW |
dc.description.abstract | Receptor for activated C kinase 1 (RACK1) is a gene that is highly conserved and ubiquitous in eukaryotes. There are three RACK1 genes in Arabidopsis namely, RACK1A (At1g18080), RACK1B (At1g48630) and RACK1C (At3g18130), but AtRACK1A acts as a predominant gene. AtRACK1A is a scaffold protein and interacts with hundreds of proteins to regulate multiple pathways, including abscisic acid (ABA) signaling pathway, plant growth and development. AtRACK1A is known as a negative regulator in ABA signaling pathway. According to a previous study, additional ten amino acids of RACK1 protein were found only in dicots. However, the function of this region is still unknown. RACK1A-XRA overexpression lines (AtRACK1A without these ten amino acids), rack1a mutant line and RACK1A overexpression lines were established to compared their gene expression and phenotype with ABA treatment. The primary root length and stomatal aperture of RACK1A transgenic lines and XRA lines have a phenotype similar to rack1a under ABA treatment. The result indicates that these ten amino acids of RACK1A is related to ABA signaling. On the other hand, Translationally Controlled Tumor Protein 1 (TCTP1) is observed as an interacting protein of RACK1A, and TCTP1 is a regulator of Target of Rapamycin (TOR) pathway and is involved in plant growth. In the present study, leaf size of XRA lines and RACK1A-S286A lines under normal conditions were significantly smaller than WT. With AZD8055 (inhibitor of TOR pathway) treatment, rack1a exhibited hyposensitivity to this reagent in primary root elongation. These suggest that RACK1A may be involved
in TOR pathway, which regulates normal plant growth and development. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:02:24Z (GMT). No. of bitstreams: 1 ntu-107-R04b42016-1.pdf: 3155718 bytes, checksum: 128d91981fe4f7140cc5b3414c1d9709 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 論文口試委員審定書(i)
誌謝(ii) 摘要(iii) Abstract(iv) Contents(v) List of Figures(viii) List of Appendixes(ix) List of Abbreviations(x) 1.Introduction(1) 1.1 Receptor for activated C kinase 1 (RACK1)(1) 1.1.1 Arabidopsis RACK1 gene family(1) 1.1.2 Molecular structure of RACK1(1) 1.1.3 RACK1 associates with ribosomes(2) 1.1.4 Biological functions of RACK1 gene(2) 1.2 Phytohormone ABA(3) 1.2.1 ABA in osmotic stress response(3) 1.2.2 Stomatal opening and closure in response to ABA treatment(4) 1.3 Leaf development(5) 1.4 Target of rapamycin pathway (TOR pathway)(5) 1.4.1 Plant TOR pathway(5) 1.4.2 TOR inhibitors(6) 1.4.3 Translationally controlled tumor protein (TCTP)(6) 1.5 Project goals(7) 2.Materials and Methods(9) 2.1 Plant materials and growth conditions(9) 2.2 Generation of RACK1A complementation lines(9) 2.3 E.coli transformation(10) 2.4 Agrobacterium-mediated floral dip transformation(10) 2.4.1 Agrobacterium transformation(10) 2.4.2 Floral dipping(10) 2.5 RNA extraction and real-time PCR analysis(11) 2.6 Leaf water loss assay(11) 2.7 Root growth assay(11) 2.8 Stomatal closure assay with ABA treatment(12) 2.9 Bimolecular fluorescence complementation (BiFC) assay(12) 2.9.1 Plasmid construct for BiFC analysis(12) 2.9.2 BiFC analysis using Arabidopsis mesophyll protoplasts(12) 2.9.3 BiFC analysis in N. benthamiana(13) 3.Results(14) 3.1 Sequence alignment of RACK1 proteins(14) 3.2 Isolation of rack1a T-DNA insertional mutant line(14) 3.3 Generation of RACK1A, RACK1A-S286, RACK1A-XRA overexpression lines and RACK1A complementation lines(15) 3.4 Gene expression of ABA-related genes in rack1a and RACK1A transgenic lines with ABA treatment(16) 3.5 Water loss and stomatal aperture of rack1a and RACK1A transgenic lines(16) 3.6 Primary root length of rack1a and RACK1A transgenic lines with ABA treatment(17) 3.7 Leaf phenotype of rack1a and RACK1A transgenic lines under normal condition(17) 3.8 Physical interaction between RACK1A, TCTP1 and 14-3-3ω detected in BiFC assay(18) 3.9 Phenotypes of rack1a and RACK1A transgenic lines treated with asTORis(19) 4.Discussion(21) References(25) Figures(35) Appendixes(57) | |
dc.language.iso | en | |
dc.title | 阿拉伯芥RACK1A之延長片段在離層酸反應及葉片發育之功能性研究 | zh_TW |
dc.title | Functional study of the extension region of Receptor for Activated C Kinase 1 (RACK1) in abscisic acid response and leaf development in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄭萬興,張孟基,謝旭亮,靳宗洛 | |
dc.subject.keyword | RACK1,氣孔,離層酸,TOR路徑,TCTP, | zh_TW |
dc.subject.keyword | RACK1,stomatal aperture,abscisic acid,TOR pathway,TCTP, | en |
dc.relation.page | 71 | |
dc.identifier.doi | 10.6342/NTU201900304 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-01-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 3.08 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。