手動風扇式血液血漿分離平臺

Abstract

本研究開發不需電力之手動風扇式血液血漿分離平台。利用3D列印技術搭配齒輪機構，以手動的方式帶動平台旋轉產生離心力使血液血漿分離，並藉由流場的設計使血液血漿分離效率進一步提升。我們測試了在不同旋轉半徑 (R)、水平偏擺角度 (θ)、垂直偏擺角度 (φ)的條件下的旋轉分離情況，並記錄血漿的產率隨著時間的變化，接著最佳化了此平台的轉速區間、幾何尺寸、旋轉方向等參數，我們亦測試了平台的穩定性，包括比較不同血液的分離產率以及純度，最後測試不同使用者在使用本平台的使用情況。結果顯示，本研究開發之平台，以一秒鐘手持曲柄轉一圈的轉速輸入即可使平台輸出2500 rpm以上的轉速，並在旋轉半徑 (R)為25 mm、水平偏擺角度 (θ)為60°、垂直偏擺角度 (φ)為0°、管內徑1 mm有最佳的分離效率，只需150 sec便能使分離後血液之血漿產率及純度達40 %以上與99.9 %以上，且具有良好的穩定性，適用於不同血液以及不同的使用者與不同的血液樣品量。期望未來可運用於資源匱乏區域之醫療前處理。

This study developed a manual handheld fan-based blood plasma separation platform without using any electricity. The 3D printing technique and gear mechanism are used to fabricate the platform to generate centrifugal force by simply turning the crank to separate blood plasma. In additon, we designed the structure with different flow fields to improve blood separation efficiency. We then tested the different radii (R), horizontal yaw angle (θ), and vertical yaw angle (φ) and recorded the change in plasma yield over time. Meanwhile, we also tested the stability of the platform, including the separation yield, purity of different blood, and operating by different users. The results show that the platform can output more than 2500 RPM by gently turning the crank, and when R = 25 mm, θ = 60°, φ = 0°, and the tube inner diameter is 1 mm has the best separation efficiency. The plasma yield and purity of the separated blood can reach at least 40% and 99.9% purity in 150 seconds. It also showed good stability of the separation results from different blood samples and different users. We can expect that the platform can be further applied to blood-based disease diagnosis in resource-limited regions.