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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡孟勳 | |
dc.contributor.author | Li-Yu Chuang | en |
dc.contributor.author | 莊立宇 | zh_TW |
dc.date.accessioned | 2021-06-17T06:01:31Z | - |
dc.date.available | 2024-02-14 | |
dc.date.copyright | 2019-02-14 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-01-31 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71480 | - |
dc.description.abstract | 第二型糖尿病是一種主要受遺傳和環境因素所影響的複雜疾病,許多因素包括年齡生活方式和飲食習慣都會影響糖尿病的形成,現今已經成為全世界主要的公眾議題。根據世衛組織的報告,2014年全世界估計有4.22億成年人患有糖尿病,人數是三十多年前的四倍。最近的研究顯示腸道微生物群對於宿主健康的重要性,許多疾病可能有微生物組成失調的情況,包括第二型糖尿病在內的代謝疾病。然而,先前糖尿病相關的研究僅探討正常人和糖尿病患者之間腸道細菌組成的差異,但是細菌對糖尿病病情嚴重程度的影響則尚未研究。微生物早期研究的策略是將單一細菌進行純化和培養,但在人體中存在大量不能被純化培養的微生物。隨著次世代定序的發展,我們可以使用宏基因組方法來分析特定環境中的微生物群組成。因此在本研究中,我們使用344個漢人糞便樣本全基因組定序的公開數據,來研究腸道微生物群豐度變化與糖尿病嚴重程度之間的關聯性。首先,我們建立了一個全基因組定序數據研究的流程來分析腸道微生物,我們從原始定序片段中進行物種分類並且透過統計分析找到一些跟糖尿病嚴重程度有關的細菌特徵,經過篩選過後我們找到兩株可能為潛在生物特徵的菌種.隨後我們構建共同豐度網絡來觀察細菌之間的交互作用以及物種失調的現象是否存在於我們的研究樣本中。為了得到功能分析所需要的組裝序列,我們還進行宏基因組的組裝並且進行評估。最後我們完成對於每個組別之中的基因預測,並且發現在糖尿病嚴重程度不同的病人中存在著某些功能差異。本研究不僅為宏基因組學分析提供了新的分析流程,並且發掘腸道微生物的組成與糖尿病嚴重程度之間關聯性的更多細節。 | zh_TW |
dc.description.abstract | Type 2 diabetes (T2D) is a complex disorder mainly influenced by both genetic and environmental components, including age, lifestyle and diet, has become a major public issue worldwide. According to the WHO report, there were around 422 million adults worldwide suffering from diabetes in 2014, fourth times more than three decades ago. Recent studies suggested that gut microbiota, which may link to metabolic diseases including T2D has become more important. However, these previous studies mainly focus on bacteria composition differences between normal subjects and diabetic patients, but the effects of bacteria on severity of T2D were still unclear. With the power of next-generation sequencing technology, we can use metagenomic approach to analyze microbiota composition in certain environments. Here, we used a public dataset whichh as fecal shotgun sequencing data from 344 Chinese individuals to characterize the association of gut microbiota abundance with severity of diabetes. We constructed a pipeline for a metagenomic association study. First, we applied taxonomic species-level analysis from raw reads and used statistical method to find several differential markers in different severity of T2D. After filtered and evaluated our findings, we reported two potential bacteria species in our study. Subsequently, we constructed co-abundance networks to investigate whether dysbiosis phenomenon exist in our sample. Furthermore, we performed de novo assembly and quality evaluation to examine the performance of different assemblers in metagenomics sequencing data. At last, we finished gene prediction and find some functional drift between different status in T2D. This study not only provides a new pipeline in metagenomics analysis but also explore more detail with the association between gut microbial composition and the severity of T2D. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:01:31Z (GMT). No. of bitstreams: 1 ntu-108-R05642007-1.pdf: 2143365 bytes, checksum: f9a779aa194a3600e65e944dd1386189 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書 #
中文摘要 I Abstract III 表目錄 VII 圖目錄 VIII 第一章介紹 1 1-1第二型糖尿病 1 1-2定序技術 2 1-2-1 桑格定序法 2 1-2-2 次世代定序 3 1-3 宏觀基因組學 4 1-3-1 16S核醣體擴增法定序 4 1-3-2 全基因組散彈槍定序法 5 1-4宏觀基因組學和疾病 5 1-4-1 宏觀基因組學對於二型糖尿病的研究 6 1-5 研究目標 8 第二章 材料與方法 9 2-1樣本數據集以及分組 9 2-2數據預處理 9 2-3 De novo 組裝以及品質評估 10 2-4物種分類 10 2-5基因預測,註釋以及豐富度計算 11 2-6統計分析 11 2-7微生物多樣性計算及共豐富度網絡構建 12 第三章結果 13 3-1 宏觀基因組資料集 13 3.2 差異菌種分析 14 3-3內部驗證 15 3-4微生物多樣性差異及共豐富度網絡 16 3-5 宏觀基因組組裝工具選擇 17 第四章 討論 20 4-1 宏基因組組裝器評估 20 4-2糞便中潛在生物特徵與二型糖尿病患者血糖和血脂水平的關聯性 21 4-3微生物多樣性和豐富度網絡 22 4-4血糖和血脂水平中微生物功能差異分析 24 4-5實驗限制 25 第五章 結論 27 第六章 文獻回顧 28 | |
dc.language.iso | zh-TW | |
dc.title | 探討糖尿病人腸胃細菌對於血糖血脂調控之關聯性 | zh_TW |
dc.title | Investigate the Association Between Microbiomeand Blood Glucose and Lipid Regulation in Type Two Diabetes Patients | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蕭自宏,莊曜宇,盧子彬,賴亮全 | |
dc.subject.keyword | 宏基因組,第二型糖尿病,基因組定序,基因組裝,豐度差異分析, | zh_TW |
dc.subject.keyword | Metagenomics,Type 2 diabetes,Whole genome sequencing,De novo assembly,Differential abundance analysis, | en |
dc.relation.page | 61 | |
dc.identifier.doi | 10.6342/NTU201900356 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-02-10 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
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