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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 林淑華(Shu-Wha Lin) | |
dc.contributor.author | Kuan-Ying Chien | en |
dc.contributor.author | 錢冠穎 | zh_TW |
dc.date.accessioned | 2021-06-17T06:00:34Z | - |
dc.date.available | 2024-03-05 | |
dc.date.copyright | 2019-03-05 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-02-12 | |
dc.identifier.citation | Ameer F, Scandiuzzi L, Hasnain S, Kalbacher H, Zaidi N.De novo lipogenesis in health and disease. Metabolism. 2014 Jul;63(7):895-902.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71429 | - |
dc.description.abstract | Gene L的生理功能未明,而本篇研究主要利用實驗室已建立的gene L 突變鼠,探討其生理功能,由於gene L突變鼠的外觀、體重與繁殖能力正常,因此進行健康檢查,分析肝功能、腎功能、血球及血脂,檢驗項目包括突變鼠禁食六小時後血糖、三酸甘油脂、總膽固醇、低密度脂蛋白、高密度脂蛋白濃度以及胰島素和葡萄糖耐受性試驗。本研究發現,gene L突變鼠血清丙胺酸轉胺酶和天門冬胺酸轉胺酶正常,表示突變鼠肝臟無明顯損傷。全血球分析結果顯示,gene L突變鼠的血小板數量異常但促血小板生成素無變化,因此未來需更多實驗探討血小板上升原因。接著利用H&E染色觀察到突變鼠肝臟有部分大顆囊泡類型的脂肪變性,並以Oil red O染色確認脂肪堆積於部分肝臟。生化分析顯示,gene L突變鼠血清三酸甘油脂、總膽固醇、低密度脂蛋白和高密度脂蛋白的濃度比野生型對照組高,然而肝臟總三酸甘油脂和總膽固醇含量正常。結合組織病理學結果和血清脂肪含量上升,懷疑突變鼠有可能發展成第二型糖尿病,因此檢測血糖並進行葡萄糖或胰島素耐受性試驗。結果顯示gene L突變鼠禁食血糖與野生型對照組之間無差異,灌食葡萄糖耐受性試驗結果亦無異常,但注射胰島素三十分鐘後對低血糖的反應較快。綜合上述結果,gene L突變鼠的肝臟脂肪代謝異常,進而可能發展成非酒精性脂肪肝。 | zh_TW |
dc.description.abstract | The physiological function of gene L remains unclear, so the gene L mutant mice were produced to investigate. The gene L mutant mice were fertile and grew normally, and then the biomarkers of liver function, renal function, blood and serum lipids in the gene L mutant mice were analyzed, including the fasting blood concentration of glucose, triglycerides, cholesterols, low density lipoproteins (LDLs), high density lipoproteins (HDLs) and the insulin resistance and glucose tolerance tests. The alanine transaminase (ALT) and aspartate transaminase (AST) in the gene L mutant mice were normal indicating they had no liver injury. According to the CBC results, the platelet count in the gene L mutant mice was higher than the wild-type mice, but the thrombopoietin level didn’t increase in mutant mice, so it needs further investigation. H&E stains of mutant mice liver showed they had slight macrovesicular steatosis, and then confirmed lipid accumulation in some part of mutant mice liver by Oil red O stain. The serum triglycerides, total cholesterols, LDLs and HDLs were higher in the gene L mutant mice with increased lipid droplets accumulated in the liver. However, the gene L mutant mice had the normal amount of liver triglycerides and cholesterol. The observations of histopathology and increased serum lipid levels suggested the gene L mutant mice had high risk in developing type 2 diabetic mellitus, so I measured the fasting blood glucose and performed glucose and insulin tolerance tests. The results demonstrated the gene L mutant mice had normal effect in oral glucose tolerance test, and improved in response to hypoglycemia after insulin challenges. These results suggested that the gene L mutant might cause the development of non-alcoholic fatty liver disease (NAFLD) through the alterations of hepatic lipid metabolism without inducing glucose intolerance. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:00:34Z (GMT). No. of bitstreams: 1 ntu-108-R05424037-1.pdf: 6179322 bytes, checksum: 7b6bb3d3f78601bcdce67cd7abba6eb4 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員審定書 Ⅰ
致謝 Ⅱ 中文摘要 Ⅲ 英文摘要 Ⅳ 圖目次 Ⅸ 第一章、緒論 1 1.1血清生化分析 1 1.2全血球分析 2 1.3組織病理學 3 1.4脂肪代謝 4 1.4.1肝臟脂肪代謝 4 1.4.2肝臟脂肪微滴 5 1.4.3內源性脂蛋白路徑 6 1.4.4高密度脂蛋白代謝 6 1.5 血糖平衡 7 1.6 研究目的 8 第二章、實驗方法與材料 9 2.1 實驗動物 9 2.1.1 實驗動物來源 9 2.1.2 實驗動物犧牲 9 2.2 樣品處理與保存 9 2.2.1 組織檢體處理 9 2.2.2 石蠟包埋與切片 9 2.2.3 冷凍包埋與切片 9 2.3 病理分析 10 2.3.1 H&E染色和Masson’s trichrome染色 10 2.3.2 Oil red O染色 10 2.3.3 血清生化分析 11 2.3.4 全血球計數 11 2.4 電子顯微鏡 11 2.4.1 檢體前處理與固定 11 2.4.2 樣品包埋 11 2.5 小鼠肝臟脂肪含量 12 2.5.1 肝臟脂肪萃取 12 2.5.2 肝臟脂肪分析 13 2.6 評估小鼠胰島素抗性試驗 13 2.6.1腹腔注射葡萄糖耐受性試驗 13 2.6.2 腹腔注射胰島素耐受性試驗 13 2.6.3 口服葡萄糖耐受性試驗 14 2.7 數據分析與統計 15 第三章、實驗結果 16 3.1 Gene L突變鼠無肝損傷且腎功能正常 16 3.2 Gene L突變鼠血小板增加 17 3.3 Gene L突變鼠肝臟組織病理學異常 19 3.4 Gene L突變鼠血清脂質與脂蛋白含量升高但肝臟脂肪含量正常 20 3.5 Gene L突變鼠的胰島素耐受性試驗異常 22 第四章、討論 24 4.1突變鼠與野生型對照組的血小板 24 4.2 組織染色結果 24 4.3 電子顯微鏡結果 25 4.4 Oil red O染色與肝臟三酸甘油脂和膽固醇含量相反 25 4.5 胰島素耐受性試驗結果 26 4.6 腹腔注射與灌食葡萄糖耐受性試驗結果比較 26 第五章、實驗結論與展望 27 參考文獻 29 | |
dc.language.iso | zh-TW | |
dc.title | Gene L突變小鼠的特性 | zh_TW |
dc.title | Phenotypic characterization of the gene L mutant mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳瑞菁,陳健弘,林淑容 | |
dc.subject.keyword | 突變鼠, | zh_TW |
dc.subject.keyword | Gene L,Phenotypic characterization, | en |
dc.relation.page | 52 | |
dc.identifier.doi | 10.6342/NTU201900365 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-02-12 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
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