請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71380
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林金全 | |
dc.contributor.author | Ya-Chi Huang | en |
dc.contributor.author | 黃雅琦 | zh_TW |
dc.date.accessioned | 2021-06-17T05:59:46Z | - |
dc.date.available | 2024-02-14 | |
dc.date.copyright | 2019-02-14 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-02-13 | |
dc.identifier.citation | 1. Nagase, H., Matrix metalloproteinases. In Zinc metalloproteases in health and disease, CRC Press: 2014; pp 173-224.
2. Vu, T. H.; Werb, Z., Matrix metalloproteinases: effectors of development and normal physiology. Genes & development 2000, 14 (17), 2123-2133. 3. Visse, R.; Nagase, H., Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases. Circulation Research 2003, 92 (8), 827-839. 4. Parks, W. C.; Wilson, C. L.; López-Boado, Y. S., Matrix metalloproteinases as modulators of inflammation and innate immunity. Nature Reviews Immunology 2004, 4, 617. 5. Burrage, P. S.; Mix, K. S.; Brinckerhoff, C. E., Matrix metalloproteinases: role in arthritis. Front Biosci 2006, 11 (1), 529-543. 6. Itoh, Y.; Nagase, H., Matrix metalloproteinases in cancer. Essays in biochemistry 2002, 38, 21-36. 7. Rundhaug, J. E., Matrix Metalloproteinases, Angiogenesis, and Cancer: Commentary re: AC Lockhart et al., Reduction of Wound Angiogenesis in Patients Treated with BMS-275291, a Broad Spectrum Matrix Metalloproteinase Inhibitor. Clin. Cancer Res., 9: 00–00, 2003. Clinical Cancer Research 2003, 9 (2), 551-554. 8. Incorvaia, L.; Badalamenti, G.; Rini, G.; Arcara, C.; Fricano, S.; Sferrazza, C.; Di Trapani, D.; Gebbia, N.; Leto, G., MMP-2, MMP-9 and activin A blood levels in patients with breast cancer or prostate cancer metastatic to the bone. Anticancer research 2007, 27 (3B), 1519-1525. 9. Gialeli, C.; Theocharis, A. D.; Karamanos, N. K., Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting. The FEBS journal 2011, 278 (1), 16-27. 10. Jonsson, A.; Hjalmarsson, C.; Falk, P.; Ivarsson, M.-L., Levels of matrix metalloproteinases differ in plasma and serum – aspects regarding analysis of biological markers in cancer. British Journal Of Cancer 2016, 115, 703. 11. Egeblad, M.; Werb, Z., New functions for the matrix metalloproteinases in cancer progression. Nature Reviews Cancer 2002, 2, 161. 12. Tlatli, R.; El Ayeb, M., MMP inhibitors and cancer treatment trials, limitations and hopes for the future. Archives de l'Institut Pasteur de Tunis 2013, 90 (1-4), 3. 13. Cathcart, J.; Pulkoski-Gross, A.; Cao, J., Targeting matrix metalloproteinases in cancer: bringing new life to old ideas. Genes & diseases 2015, 2 (1), 26-34. 14. Zhong, Y.; Lu, Y.-T.; Sun, Y.; Shi, Z.-H.; Li, N.-G.; Tang, Y.-P.; Duan, J.-A., Recent opportunities in matrix metalloproteinase inhibitor drug design for cancer. Expert opinion on drug discovery 2018, 13 (1), 75-87. 15. Yue, B., Biology of the extracellular matrix: an overview. Journal of glaucoma 2014, S20. 16. Pieper-Fürst, U.; Kleuser, U.; Stöcklein, W. F. M.; Warsinke, A.; Scheller, F. W., Detection of subpicomolar concentrations of human matrix metalloproteinase-2 by an optical biosensor. Analytical Biochemistry 2004, 332 (1), 160-167. 17. Patel, S.; Sumitra, G.; Koner, B. C.; Saxena, A., Role of serum matrix metalloproteinase-2 and -9 to predict breast cancer progression. Clinical Biochemistry 2011, 44 (10), 869-872. 18. Barbosa Jr., F.; Gerlach, F.; Tanus-Santos, J. E., Matrix Metalloproteinase-9 Activity in Plasma Correlates with Plasma and Whole Blood Lead Concentrations. Basic & Clinical Pharmacology & Toxicology 2006, 98 (6), 559-564. 19. Yamane, T.; Mitsumata, M.; Yamaguchi, N.; Nakazawa, T.; Mochizuki, K.; Kondo, T.; Kawasaki, T.; Murata, S.-i.; Yoshida, Y.; Katoh, R., Laminar high shear stress up-regulates type IV collagen synthesis and down-regulates MMP-2 secretion in endothelium. A quantitative analysis. Cell and Tissue Research 2010, 340 (3), 471-479. 20. Jung, S.-H.; Kong, D.-H.; Park, J. H.; Lee, S.-T.; Hyun, J.; Kim, Y.-M.; Ha, K.-S., Rapid analysis of matrix metalloproteinase-3 activity by gelatin arrays using a spectral surface plasmon resonance biosensor. Analyst 2010, 135 (5), 1050-1057. 21. Bolduc, O. R.; Pelletier, J. N.; Masson, J.-F., SPR Biosensing in Crude Serum Using Ultralow Fouling Binary Patterned Peptide SAM. Analytical Chemistry 2010, 82 (9), 3699-3706. 22. Shin, D.-S.; Liu, Y.; Gao, Y.; Kwa, T.; Matharu, Z.; Revzin, A., Micropatterned Surfaces Functionalized with Electroactive Peptides for Detecting Protease Release from Cells. Analytical Chemistry 2013, 85 (1), 220-227. 23. Fan, G.-C.; Han, L.; Zhu, H.; Zhang, J.-R.; Zhu, J.-J., Ultrasensitive Photoelectrochemical Immunoassay for Matrix Metalloproteinase-2 Detection Based on CdS:Mn/CdTe Cosensitized TiO2 Nanotubes and Signal Amplification of SiO2@Ab2 Conjugates. Analytical Chemistry 2014, 86 (24), 12398-12405. 24. Lei, Z.; Gao, J.; Liu, X.; Liu, D.; Wang, Z., Peptide microarray-based fluorescence assay for simultaneously detecting matrix metalloproteinases. Analytical Methods 2016, 8 (1), 72-77. 25. Lei, Z.; Gao, J.; Liu, X.; Liu, D.; Wang, Z., Poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) Brushes as Peptide/Protein Microarray Substrate for Improving Protein Binding and Functionality. ACS Applied Materials & Interfaces 2016, 8 (16), 10174-10182. 26. Lei, Z.; Chen, H.; Zhang, H.; Wang, Y.; Meng, X.; Wang, Z., Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment. ACS Applied Materials & Interfaces 2017, 9 (50), 44241-44250. 27. O’Keefe, A.; Deacon, D. A. G., Cavity ring‐down optical spectrometer for absorption measurements using pulsed laser sources. Review of Scientific Instruments 1988, 59 (12), 2544-2551. 28. Scherer, J. J.; Paul, J. B.; O'Keefe, A.; Saykally, R. J., Cavity Ringdown Laser Absorption Spectroscopy: History, Development, and Application to Pulsed Molecular Beams. Chemical Reviews 1997, 97 (1), 25-52. 29. Berden, G.; Peeters, R.; Meijer, G., Cavity ring-down spectroscopy: Experimental schemes and applications. International Reviews in Physical Chemistry 2000, 19 (4), 565-607. 30. Hallock, A. J.; Berman, E. S. F.; Zare, R. N., Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy. Analytical Chemistry 2002, 74 (7), 1741-1743. 31. Hallock, A. J.; Berman, E. S. F.; Zare, R. N., Ultratrace Kinetic Measurements of the Reduction of Methylene Blue. Journal of the American Chemical Society 2003, 125 (5), 1158-1159. 32. Xu, S.; Sha, G.; Xie, J., Cavity ring-down spectroscopy in the liquid phase. Review of Scientific Instruments 2002, 73 (2), 255-258. 33. Snyder, K. L.; Zare, R. N., Cavity Ring-Down Spectroscopy as a Detector for Liquid Chromatography. Analytical Chemistry 2003, 75 (13), 3086-3091. 34. Pipino, A. C. R.; Hudgens, J. W.; Huie, R. E., Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity. Review of Scientific Instruments 1997, 68 (8), 2978-2989. 35. Pipino, A. C. R.; Hudgens, J. W.; Huie, R. E., Evanescent wave cavity ring-down spectroscopy for probing surface processes. Chemical Physics Letters 1997, 280 (1), 104-112. 36. Schnippering, M.; Neil, S. R.; Mackenzie, S. R.; Unwin, P. R., Evanescent wave cavity-based spectroscopic techniques as probes of interfacial processes. Chem Soc Rev 2011, 40 (1), 207-20. 37. Shaw, A. M.; Hannon, T. E.; Li, F.; Zare, R. N., Adsorption of Crystal Violet to the Silica−Water Interface Monitored by Evanescent Wave Cavity Ring-Down Spectroscopy. The Journal of Physical Chemistry B 2003, 107 (29), 7070-7075. 38. Li, F.; Zare, R. N., Molecular Orientation Study of Methylene Blue at an Air/Fused-Silica Interface Using Evanescent-Wave Cavity Ring-Down Spectroscopy. The Journal of Physical Chemistry B 2005, 109 (8), 3330-3333. 39. Fan, H.-F.; Hung, C.-Y.; Lin, K.-C., Molecular Adsorption at Silica/CH3CN Interface Probed by Using Evanescent Wave Cavity Ring-Down Absorption Spectroscopy: Determination of Thermodynamic Properties. Analytical Chemistry 2006, 78 (11), 3583-3590. 40. Fan, H.-F.; Li, F.; Zare, R. N.; Lin, K.-C., Characterization of Two Types of Silanol Groups on Fused-Silica Surfaces Using Evanescent-Wave Cavity Ring-Down Spectroscopy. Analytical Chemistry 2007, 79 (10), 3654-3661. 41. Chen, M.-S.; Fan, H.-F.; Lin, K.-C., Kinetic and Thermodynamic Investigation of Rhodamine B Adsorption at Solid/Solvent Interfaces by Use of Evanescent-Wave Cavity Ring-Down Spectroscopy. Analytical Chemistry 2010, 82 (3), 868-877. 42. Lin, M.-C.; Lin, K.-C., Interaction between crystal violet and anionic surfactants at silica/water interface using evanescent wave-cavity ring-down absorption spectroscopy. Journal of Colloid and Interface Science 2012, 379 (1), 41-47. 43. Yao, Y. J.; Lin, K. C., DNA interaction probed by evanescent wave cavity ring-down absorption spectroscopy via functionalized gold nanoparticles. Anal Chim Acta 2014, 820, 1-8. 44. contributors, W. Total internal reflection. https://en.wikipedia.org/w/index.php?title=Total_internal_reflection&oldid=878816372 (accessed 17 January 2019 07:09 UTC). 45. Newport Spatial Filters. https://www.newport.com/n/spatial-filters. 46. Chivers, Claire E.; Koner, Apurba L.; Lowe, Edward D.; Howarth, M., How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer. Biochemical Journal 2011, 435 (1), 55-63. 47. McFarland, A. D.; Haynes, C. L.; Mirkin, C. A.; Van Duyne, R. P.; Godwin, H. A., Color My Nanoworld. Journal of Chemical Education 2004, 81 (4), 544A. 48. Samokhvalov, A. V.; Razo, S. C.; Safenkova, I. V.; Slutskaya, E. S.; Pridvorova, S. M.; Zherdev, A. V.; Dzantiev, B. B., Synthesis of the Most Effective Streptavidin Conjugates with Small Gold Nanoparticles for Indirect Labeling in Lateral Flow Assay. International Journal of Applied Engineering Research 2017, 12 (23), 13847-13860. 49. Kridel, S. J.; Chen, E.; Kotra, L. P.; Howard, E. W.; Mobashery, S.; Smith, J. W., Substrate hydrolysis by matrix metalloproteinase-9. J Biol Chem 2001, 276 (23), 20572-8. 50. Rafal, F.; Amalendu, P. R.; Anindita, M.; Jamboor, K. V.; Zygmunt, G.; Julian, B.; Pabak, S.; Ignacy, G., Fluorescence Detection of MMP-9. I. MMP-9 Selectively Cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide. Current Pharmaceutical Biotechnology 2011, 12 (5), 834-838. 51. Rafal, F.; Ryan, R.; Anindita, M.; Amalendu, P. R.; Jamboor, K. V.; Anna, K. K.; Zygmunt, G.; Julian, B.; Ignacy, G., Fluorescence Detection of MMP-9. II. Ratiometric FRET-Based Sensing With Dually Labeled Specific Peptide. Current Pharmaceutical Biotechnology 2013, 14 (13), 1134-1138. 52. Li, N.; Yi, L.; He, Z.; Zhang, W.; Li, H.; Lin, J.-M., A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases. Analyst 2017, 142 (4), 634-640. 53. Lee, J.; Samson, A. A. S.; Song, J. M., Peptide substrate-based inkjet printing high-throughput MMP-9 anticancer assay using fluorescence resonance energy transfer (FRET). Sensors and Actuators B: Chemical 2018, 256, 1093-1099. 54. Shih, H.; Lin, C.-C., Visible-Light-Mediated Thiol-Ene Hydrogelation Using Eosin-Y as the Only Photoinitiator. Macromolecular Rapid Communications 2013, 34 (3), 269-273. 55. Shih, H.; Fraser, A. K.; Lin, C.-C., Interfacial Thiol-ene Photoclick Reactions for Forming Multilayer Hydrogels. ACS Applied Materials & Interfaces 2013, 5 (5), 1673-1680. 56. Lefevre, C.; Kang, H. C.; Haugland, R. P.; Malekzadeh, N.; Arttamangkul, S.; Haugland, R. P., Texas Red-X and Rhodamine Red-X, New Derivatives of Sulforhodamine 101 and Lissamine Rhodamine B with Improved Labeling and Fluorescence Properties. Bioconjugate Chemistry 1996, 7 (4), 482-489. 57. Liu, X.; Atwater, M.; Wang, J.; Huo, Q., Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids and Surfaces B: Biointerfaces 2007, 58 (1), 3-7. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71380 | - |
dc.description.abstract | 本研究是利用漸逝波腔體震盪吸收光譜法(evanescent-wave cavity ring-down spectroscopy, EW-CRDS)結合金奈米粒子(gold nanoparticles, Au NPs)與染料rhodamine redTM-X (RRX)作為探針來偵測基質金屬蛋白酶9 (MMP-9)。基質金屬蛋白酶9為人體蛋白酶,其可降解細胞外基質的第四型膠原蛋白。研究發現基質金屬蛋白酶9與腫瘤細胞的轉移有著正相關。罹患癌症的病人其體內有著濃度高於正常人的基質金屬蛋白酶9,因此基質金屬蛋白酶9被視為一種癌症的生物指標。
腔體震盪吸收光譜法的原理是由高反射鏡所構成的腔體來回反射脈衝雷射,並測量由腔體洩漏的能量,將其轉化成腔體震盪時間(ring-down time)。光在腔體內來回反射次數再搭配上腔體的長度,其有效的吸收路徑可達到數公里,可用來偵測微量物質,具有相當高的靈敏性。當腔體內被放置一特殊設計的稜鏡,稜鏡表面因雷射光經全反射所產生的漸逝波可被用來偵測稜鏡表面的光學吸收變化,而此方法被稱作漸逝波腔體震盪吸收光譜法。 本實驗分別使用在表面修飾上鏈親和素(streptavidin)的金奈米粒子與rhodamine redTM-X作為探針。因鏈親和素與生物素(biotin)具有極強的非共價作用力,所以我們在玻片表面修飾上具有生物素的胜肽鏈,並將此玻片放置於產生漸逝波的稜鏡上。而此胜肽鏈對基質金屬蛋白酶9有著非常好的專一性,並且能被其水解成片段。所以當玻片表面的胜肽鏈被基質金屬蛋白酶9水解,玻片表面能吸引修飾上鏈親和素之探針的生物素就會減少,使得吸收改變。漸逝波腔體震盪吸收光譜法可藉此表面之吸收變化,來對基質金屬蛋白酶9進行偵測。 | zh_TW |
dc.description.abstract | Matrix metallopeptide 9 (MMP-9) belongs to a family of zinc-dependent endopeptidases for degrading the extracellular matrix in human body. MMP-9 has been proven to be a cancer biomarker as MMP-9 is able to help tumor metastasis, and the concentrations of MMP-9 in cancer patients are higher than healthy people. On the other hand, cavity-ring down spectroscopy (CRDS) is an ultra-sensitive method in detecting trace molecules due to long absorption length by multiple reflections inside a cavity composed of highly reflective mirrors. With a customized prism placing inside the cavity, evanescent wave is generated by total internal reflection at the prism surface and able to detect absorption changes. The surface-sensitive method is called evanescent-wave cavity-ring down spectroscopy (EW-CRDS). In this work, EW-CRDS was employed to detect MMP-9 by using two kinds of streptavidin-conjugated probes, gold nanoparticles (AuNPs) and rhodamine redTM-X (RRX) dye, to bind with biotin-labeled peptides on the surface of the prism. This study provides a further sensing method with EW-CRDS. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T05:59:46Z (GMT). No. of bitstreams: 1 ntu-108-R04223194-1.pdf: 2785282 bytes, checksum: 0d4e2753285c8fdadf428e6f009065e8 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii Contents iv Figure Captions vi Table Caption ix Chapter 1 Introduction 1 Chapter 2 Evanescent wave Cavity Ring-down Spetroscopy 3 2.1 Cavity Ring-down Spectroscopy 3 2.2 History, Development and Applications 5 2.3 Evanescent-wave Cavity Ring-Down Spectroscopy 7 2.4 The Principle of EW-CRDS 9 2.5 Solving for the Absolute Absorbance 10 2.6 Total Internal Reflection 13 2.7 Brewster’s Angle 13 Chapter 3 Experimental Section 15 3.1 Experimental Setup 15 3.1.1 Laser system 15 3.1.2 Spatial Filter 16 3.1.3 Optical Cavity 16 3.1.4 Data Acquisition 18 3.2 Experimental Sensing Strategy 19 3.3 Experimental Preparation 20 3.3.1 Streptavidin-conjugated Probes Preparation 20 3.3.2 Peptide Preparation 23 3.3.3 Peptide-coated Coverslips Preparation 24 3.3.4 MMP-9-treated Peptide-coated Coverslips Preparation 25 3.4 Experimental Procedure 25 Chapter 4 Results and Discussion 27 4.1 Adsorption of Streptavidin conjugated probes on Peptide-coated coverslips…….. 27 4.1.1 Rhodamine RedTM-X-conjugated Streptavidin (RRX-SA) 27 4.1.2 Streptavidin-conjugated Gold Nanoparticles (SA-AuNPs) 29 4.2 MMP-9 Sensing 32 4.2.1 Rhodamine RedTM-X-conjugated Streptavidin (RRX-SA) 32 4.2.2 Streptavidin-conjugated Gold Nanoparticles (SA-AuNPs) 35 4.3 Summary 37 Chapter 5 Conclusions and Future works 40 Reference 41 | |
dc.language.iso | en | |
dc.title | 以金奈米粒子與染料結合漸逝波腔體震盪吸收光譜法偵測基質金屬蛋白酶9 | zh_TW |
dc.title | Detection of Matrix Metallopeptidase 9 Using Evanescent-wave Cavity Ring-down Spectroscopy:Gold Nanoparticles and Dye as Probe | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 范秀芳,黃志清 | |
dc.subject.keyword | 腔體震盪光譜,漸逝波,基質金屬蛋白?9,金奈米粒子,胜?, | zh_TW |
dc.subject.keyword | EW-CRDS,cavity-ring down spectroscopy,MMP-9 sensing,peptide,gold nanoparticles,peptides, | en |
dc.relation.page | 45 | |
dc.identifier.doi | 10.6342/NTU201900532 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-02-13 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 化學研究所 | zh_TW |
顯示於系所單位: | 化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 2.72 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。