請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7137
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃聲蘋(Sheng-Ping L. Hwang) | |
dc.contributor.author | Yu-Hsiu Liu | en |
dc.contributor.author | 劉昱秀 | zh_TW |
dc.date.accessioned | 2021-05-17T15:59:49Z | - |
dc.date.available | 2020-06-09 | |
dc.date.available | 2021-05-17T15:59:49Z | - |
dc.date.copyright | 2020-06-09 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-04-29 | |
dc.identifier.citation | Alexander, J., Rothenberg, M., Henry, G. L. and Stainier, D. Y. R. (1999). casanova Plays an Early and Essential Role in Endoderm Formation in Zebrafish. Developmental Biology 215, 343-357.
Balling, R., Deutsch, U. and Gruss, P. (1988). undulated, a mutation affecting the development of the mouse skeleton, has a point mutation in the paired box of Pax 1. Cell 55, 531-535. Barriga, E. H., Maxwell, P. H., Reyes, A. E. and Mayor, R. (2013). The hypoxia factor Hif-1α controls neural crest chemotaxis and epithelial to mesenchymal transition. Journal of Cell Biology 201, 759-776. Chen, Y. C., Liao, B. K., Lu, Y. F., Liu, Y. H., Hsieh, F. C., Hwang, P. P. and Hwang, S. L. (2019). Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition. PLOS Genetics 15, e1008058. Cheung, M., Chaboissier, M. C., Mynett, A., Hirst, E., Schedl, A. and Briscoe, J. (2005). The transcriptional control of trunk neural crest induction, survival, and delamination. Developmental Cell 8, 179-192. Choe, C. P. and Crump, J. G. (2014). Tbx1 controls the morphogenesis of pharyngeal pouch epithelia through mesodermal Wnt11r and Fgf8a. Development 141, 3583-3593. Choudhry, P., Joshi, D., Funke, B. and Trede, N. (2011). Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis. Developmental Biology 349, 483-493. Crump, J. G., Maves, L., Lawson, N. D., Weinstein, B. M. and Kimmel, C. B. (2004a). An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterning. Development 131, 5703-5716. Crump, J. G., Swartz, M. E. and Kimmel, C. B. (2004b). An integrin-dependent role of pouch endoderm in hyoid cartilage development. PLOS Biology 2, E244. David, N. B., Saint-Etienne, L., Tsang, M., Schilling, T. F. and Rosa, F. M. (2002). Requirement for endoderm and FGF3 in ventral head skeleton formation. Development 129, 4457-4468. Davison, J. M., Akitake, C. M., Goll, M. G., Rhee, J. M., Gosse, N., Baier, H., Halpern, M. E., Leach, S. D. and Parsons, M. J. (2007). Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish. Developmental Biology 304, 811-824. Dottori, M., Gross, M. K., Labosky, P. and Goulding, M. (2001). The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development 128, 4127-4138. Ferronha, T., Rabadan, M. A., Gil-Guinon, E., Le Dreau, G., de Torres, C. and Marti, E. (2013). LMO4 is an essential cofactor in the Snail2-mediated epithelial-to-mesenchymal transition of neuroblastoma and neural crest cells. The Journal of neuroscience : the official journal of the Society for Neuroscience 33, 2773-2783. Gendron-Maguire, M., Mallo, M., Zhang, M. and Gridley, T. (1993). Hoxa-2 mutant mice exhibit homeotic transformation of skeletal elements derived from cranial neural crest. Cell 75, 1317-1331. Grammatopoulos, G. A., Bell, E., Toole, L., Lumsden, A. and Tucker, A. S. (2000). Homeotic transformation of branchial arch identity after Hoxa2 overexpression. Development 127, 5355. Grocott, T., Tambalo, M. and Streit, A. (2012). The peripheral sensory nervous system in the vertebrate head: a gene regulatory perspective. Developmental Biology 370, 3-23. Herzog, W., Sonntag, C., von der Hardt, S., Roehl, H. H., Varga, Z. M. and Hammerschmidt, M. (2004). Fgf3 signaling from the ventral diencephalon is required for early specification and subsequent survival of the zebrafish adenohypophysis. Development 131, 3681-3692. Hunt, P., Gulisano, M., Cook, M., Sham, M.-H., Faiella, A., Wilkinson, D., Boncinelli, E. and Krumlauf, R. (1991). A distinct Hox code for the branchial region of the vertebrate head. Nature 353, 861-864. Hunter, M. P. and Prince, V. E. (2002). Zebrafish hox paralogue group 2 genes function redundantly as selector genes to pattern the second pharyngeal arch. Developmental Biology 247, 367-389. Jao, L. E., Wente, S. R. and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proceedings of the National Academy of Sciences of the United States of America 110, 13904-13909. Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N. and Mishina, M. (2004). A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Developmental Cell 7, 133-144. Khudyakov, J. and Bronner-Fraser, M. (2009). Comprehensive spatiotemporal analysis of early chick neural crest network genes. Developmental Dynamics 238. Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B. and Schilling, T. F. (1995). Stages of embryonic development of the zebrafish. Developmental Dynamics 203, 253-310. Kitazawa, T., Fujisawa, K., Narboux-Neme, N., Arima, Y., Kawamura, Y., Inoue, T., Wada, Y., Kohro, T., Aburatani, H., Kodama, T., et al. (2015). Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process. Developmental Biology 402, 162-174. Knight, R. D. and Schilling, T. F. (2006). Cranial neural crest and development of the head skeleton. Advances in experimental medicine and biology 589, 120-133. Labosky, P. A. and Kaestner, K. H. (1998). The winged helix transcription factor Hfh2 is expressed in neural crest and spinal cord during mouse development. Mechanisms of Development 76, 185-190. LaMonica, K., Ding, H. L. and Artinger, K. B. (2015). prdm1a functions upstream of itga5 in zebrafish craniofacial development. Genesis 53, 270-277. Liu, J. A., Wu, M. H., Yan, C. H., Chau, B. K., So, H., Ng, A., Chan, A., Cheah, K. S., Briscoe, J. and Cheung, M. (2013a). Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America 110, 2882-2887. Liu, X., Wang, H., Li, G., Huang, H. Z. and Wang, Y. Q. (2013b). The function of DrPax1b gene in the embryonic development of zebrafish. Genes & Genetic Systems 88, 261-269. McGaughran, J. M., Oates, A., Donnai, D., Read, A. P. and Tassabehji, M. (2003). Mutations in PAX1 may be associated with Klippel-Feil syndrome. European Journal of Human Genetics 11, 468-474. McKeown, S. J., Newgreen, D. F. and Farlie, P. G. (2005). Dlx2 over-expression regulates cell adhesion and mesenchymal condensation in ectomesenchyme. Developmental Biology 281, 22-37. Medeiros, D. M. and Crump, J. G. (2012). New perspectives on pharyngeal dorsoventral patterning in development and evolution of the vertebrate jaw. Developmental Biology 371, 121-135. Meulemans, D. and Bronner-Fraser, M. (2004). Gene-regulatory interactions in neural crest evolution and development. Developmental Cell 7, 291-299. Miller, C. T., Schilling, T. F., Lee, K., Parker, J. and Kimmel, C. B. (2000). sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development. Development 127, 3815-3828. Miller, C. T., Yelon, D., Stainier, D. Y. and Kimmel, C. B. (2003). Two endothelin 1 effectors, hand2 and bapx1, pattern ventral pharyngeal cartilage and the jaw joint. Development 130, 1353-1365. Minoux, M. and Rijli, F. M. (2010). Molecular mechanisms of cranial neural crest cell migration and patterning in craniofacial development. Development 137, 2605. Mise, T., Iijima, M., Inohaya, K., Kudo, A. and Wada, H. (2008). Function of Pax1 and Pax9 in the sclerotome of medaka fish. Genesis 46, 185-192. Monsoro-Burq, A. H. (2015). PAX transcription factors in neural crest development. Seminars in Cell and Developmental Biologyl 44, 87-96. Mork, L. A. and Crump, J. G. (2015). Zebrafish Craniofacial Development: A Window into Early Patterning. Current Topics in Developmental Biology 115, 235-269. Nair, S., Li, W., Cornell, R. and Schilling, T. F. (2007). Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish. Development 134, 335-345. Nichane, M., Ren, X., Souopgui, J. and Bellefroid, E. (2008). Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus. Developmental Biology 322, 368-380. Okada, K., Inohaya, K., Mise, T., Kudo, A., Takada, S. and Wada, H. (2016). Reiterative expression of pax1 directs pharyngeal pouch segmentation in medaka. Development 143, 1800-1810. Parker, H. J., Bronner, M. E. and Krumlauf, R. (2016). The vertebrate Hox gene regulatory network for hindbrain segmentation: Evolution and diversification: Coupling of a Hox gene regulatory network to hindbrain segmentation is an ancient trait originating at the base of vertebrates. BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology 38, 526-538. Pasqualetti, M., Ori, M., Nardi, I. and Rijli, F. M. (2000). Ectopic Hoxa2 induction after neural crest migration results in homeosis of jaw elements in Xenopus. Development 127, 5367. Peters, H., Wilm, B., Sakai, N., Imai, K., Maas, R. and Balling, R. (1999). Pax1 and Pax9 synergistically regulate vertebral column development. Development 126, 5399-5408. Piotrowski, T., Ahn, D. G., Schilling, T. F., Nair, S., Ruvinsky, I., Geisler, R., Rauch, G. J., Haffter, P., Zon, L. I., Zhou, Y., et al. (2003). The zebrafish van gogh mutation disrupts tbx1, which is involved in the DiGeorge deletion syndrome in humans. Development 130, 5043-5052. Piotrowski, T. and Nusslein-Volhard, C. (2000). The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio). Developmental Biology 225, 339-356. Pohl, E., Aykut, A., Beleggia, F., Karaca, E., Durmaz, B., Keupp, K., Arslan, E., Palamar, M., Yigit, G., Ozkinay, F., et al. (2013). A hypofunctional PAX1 mutation causes autosomal recessively inherited otofaciocervical syndrome. Human Genetics 132, 1311-1320. Rodrigo, I., Hill, R. E., Balling, R., Munsterberg, A. and Imai, K. (2003). Pax1 and Pax9 activate Bapx1 to induce chondrogenic differentiation in the sclerotome. Development 130, 473-482. Rogers, C. D., Saxena, A. and Bronner, M. E. (2013). Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT. Journal of Cell Biology 203, 835-847. Sanchez, R. S. and Sanchez, S. S. (2013). Characterization of pax1, pax9, and uncx sclerotomal genes during Xenopus laevis embryogenesis. Developmental Dynamics 242, 572-579. Sato, S., Ikeda, K., Shioi, G., Ochi, H., Ogino, H., Yajima, H. and Kawakami, K. (2010). Conserved expression of mouse Six1 in the pre-placodal region (PPR) and identification of an enhancer for the rostral PPR. Developmental Biology 344, 158-171. Schier, A. F. and Shen, M. M. (2000). Nodal signalling in vertebrate development. Nature 403, 385-389. Schilling, T. F. and Kimmel, C. B. (1997). Musculoskeletal patterning in the pharyngeal segments of the zebrafish embryo. Development 124, 2945-2960. Schilling, T. F., Piotrowski, T., Grandel, H., Brand, M., Heisenberg, C. P., Jiang, Y. J., Beuchle, D., Hammerschmidt, M., Kane, D. A., Mullins, M. C., et al. (1996). Jaw and branchial arch mutants in zebrafish I: branchial arches. Development 123, 329-344. Simões-Costa, M. S., McKeown, S. J., Tan-Cabugao, J., Sauka-Spengler, T. and Bronner, M. E. (2012). Dynamic and Differential Regulation of Stem Cell Factor FoxD3 in the Neural Crest Is Encrypted in the Genome. PLOS Genetics 8, e1003142. Simoes-Costa, M. and Bronner, M. E. (2015). Establishing neural crest identity: a gene regulatory recipe. Development 142, 242-257. Southard-Smith, E. M., Kos, L. and Pavan, W. J. (1998). Sox10 mutation disrupts neural crest development in Dom Hirschsprung mouse model. Nature Genetics 18, 60-64. Sperber, S. M., Saxena, V., Hatch, G. and Ekker, M. (2008). Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements. Developmental Biology 314, 59-70. Streit, A., Berliner, A. J., Papanayotou, C., Sirulnik, A. and Stern, C. D. (2000). Initiation of neural induction by FGF signalling before gastrulation. Nature 406, 74-78. Takimoto, A., Mohri, H., Kokubu, C., Hiraki, Y. and Shukunami, C. (2013). Pax1 acts as a negative regulator of chondrocyte maturation. Experimental Cell Research 319, 3128-3139. Taneyhill, L. A., Coles, E. G. and Bronner-Fraser, M. (2007). Snail2 directly represses cadherin6B during epithelial-to-mesenchymal transitions of the neural crest. Development 134, 1481. Thisse, C. and Thisse, B. (2008). High-resolution in situ hybridization to whole-mount zebrafish embryos. Nature Protocols 3, 59-69. Timmons, P. M., Wallin, J., Rigby, P. W. and Balling, R. (1994). Expression and function of Pax 1 during development of the pectoral girdle. Development 120, 2773-2785. Underhill, D. A. (2000). Genetic and biochemical diversity in the Pax gene family. Biochemistry and Cell Biology / Biochimie et Biologie Cellulaire 78, 629-638. Urasaki, A., Morvan, G. and Kawakami, K. (2006). Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition. Genetics 174, 639-649. Wahlbuhl, M., Reiprich, S., Vogl, M. R., Bosl, M. R. and Wegner, M. (2012). Transcription factor Sox10 orchestrates activity of a neural crest-specific enhancer in the vicinity of its gene. Nucleic Acids Research 40, 88-101. Walker, M. B., Miller, C. T., Coffin Talbot, J., Stock, D. W. and Kimmel, C. B. (2006). Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning. Developmental Biology 295, 194-205. Wallin, J., Eibel, H., Neubuser, A., Wilting, J., Koseki, H. and Balling, R. (1996). Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation. Development 122, 23-30. Walshe, J. and Mason, I. (2003a). Fgf signalling is required for formation of cartilage in the head. Developmental Biology 264, 522-536. Walshe, J. and Mason, I. (2003b). Unique and combinatorial functions of Fgf3 and Fgf8 during zebrafish forebrain development. Development 130, 4337-4349. Wilm, B., Dahl, E., Peters, H., Balling, R. and Imai, K. (1998). Targeted disruption of Pax1 defines its null phenotype and proves haploinsufficiency. Proceedings of the National Academy of Sciences of the United States of America 95, 8692-8697. Yelick, P. C. and Schilling, T. F. (2002). Molecular dissection of craniofacial development using zebrafish. Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists 13, 308-322. Zhang, L., Zhong, T., Wang, Y., Jiang, Q., Song, H. and Gui, Y. (2006). TBX1, a DiGeorge syndrome candidate gene, is inhibited by retinoic acid. The International Journal of Developmental Biology 50, 55-61. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7137 | - |
dc.description.abstract | 咽弓 (pharyngeal arches) 是由三個胚層所衍生的結構,且其組織間的分子相互作用是之後咽軟骨正常發育所必需的。然而,此過程的基礎機制尚未完全釐清。這篇論文主要報導斑馬魚Pax1a和Pax1b在咽囊形態形成和隨後的角鰓軟骨(ceratobranchial cartilage) 發育中具有重疊與不可或缺的功能。 pax1a和pax1b共同表現在咽囊中,運用增強子捕獲所產生的Tg(pax1b:eGFP)螢光魚進行延時攝影影像觀察進一步揭示了咽囊發育的連續性分節情形。由CRISPR-Cas9誘變產生的pax1a-/-; pax1b-/-雙突變斑馬魚胚胎的咽囊2-5無分節,囊狀外凸(outpocketings) 變小。受精後36小時(hpf)雙突變斑馬魚胚胎的咽囊2-5中也沒有fgf3,tbx1和edn1在內胚層表現。在受精後96或36小時的CRISPR突變魚胚胎與以反義嗎啉基(antisense morpholino)弱化斑馬魚pax1a和pax1b基因的胚胎中,觀察到角鰓軟骨1-4的消失以及在咽弓3-6中dlx2a和hand2表現的減少或缺乏。這些結果顯示斑馬魚Pax1a和Pax1b透過調節fgf3和tbx1的表現來調節咽囊的形態形成。此外,我們的數據支持一個假說:內胚層表現的Pax1a和Pax1b蛋白質透過Fgf3和Tbx-Edn1訊號傳導,經由調控dlx2a和hand2的表現來非自主性地調節角鰓軟骨的發育。 | zh_TW |
dc.description.abstract | Pharyngeal arches are derived from all three germ layers and molecular interactions among the tissue types are required for proper development of subsequent pharyngeal cartilages; however, the mechanisms underlying this process are not fully described. Here we report that in zebrafish, Pax1a and Pax1b have overlapping and essential functions in pharyngeal pouch morphogenesis and subsequent ceratobranchial cartilage development. Both pax1a and pax1b are co-expressed in pharyngeal pouches, and time-lapse imaging of a novel Tg(pax1b:eGFP) enhancer trap line further revealed the sequential segmental development of pharyngeal pouches. Zebrafish pax1a-/-; pax1b-/- double mutant embryos generated by CRISPR-Cas9 mutagenesis exhibit unsegmented pharyngeal pouches 2-5 with small outpocketings. Endodermal expression of fgf3, tbx1 and edn1 is also absent in pharyngeal pouches 2-5 at 36 hours post fertilization (hpf). Loss of ceratobranchial cartilage 1-4 and reduced or absent expression of dlx2a and hand2 in the pharyngeal arches 3-6 are observed in CRISPR mutant and morphant embryos that are deficient in both zebrafish pax1a and pax1b at 96 or 36 hpf. These results suggest that zebrafish Pax1a and Pax1b both regulate pharyngeal pouch morphogenesis by modulating expression of fgf3 and tbx1. Furthermore, our data support a model wherein endodermal Pax1a and Pax1b act through Fgf3 and Tbx-Edn1 signaling to non-autonomously regulate the development of ceratobranchial cartilage via expression of dlx2a and hand2. | en |
dc.description.provenance | Made available in DSpace on 2021-05-17T15:59:49Z (GMT). No. of bitstreams: 1 ntu-109-D00b41004-1.pdf: 4649314 bytes, checksum: 6bf1bffdc7711a7079d97940187f2558 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 口試委員會審定書…………………………………………………….. I
誌謝…………………………………………………………………….. II 中文摘要………………………………………………….………….... III Abstract……………………………………………………………....... IV 1. Introduction………………………………………………….…….…. 1 1.1 Craniofacial structure of zebrafish……………………………….. 1 1.2 Neural crest morphogenesis……………………………….……... 3 1.3 Pharyngeal dorsoventral patterning…………………..………...... 7 1.4 Requirement of endoderm for craniofacial cartilage formation..... 8 1.5 Pax1…………………………………………………………....... 14 1.6 The specific aims of this thesis……………………………..…... 18 2. Materials and Methods…………………………………………….... 20 2.1 Zebrafish strains and husbandry………………………...…….… 20 2.2 Generation of Tg(pax1b:eGFP) enhancer trap transgenic fish…. 20 2.3 Generation of pax1a mutants and pax1b mutants via CRISPR-Cas9 genome editing system and off-target analysis………..……..…. 21 2.4 Morpholino-mediated knockdown………………………….…... 23 2.5 Construction of pax1a-eGFP and pax1b-eGFP for testing MO knockdown specificity…………………………..……………… 24 2.6 mRNA synthesis, in situ hybridization, immunofluorescence and TUNEL……………………………………………………..…… 25 2.7 Alcian blue staining………………….………………………….. 28 2.8 Chromatin immunoprecipitation……………………………...… 28 2.9 Semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR)…………………………. 31 2.10 Western blot……………………………………………………. 34 2.11 Paraffin section……………………………………………….... 36 2.12 Image acquisition, statistics, and quantification of area………. 36 3. Results…………………………………………………………….… 38 3.1 zebrafish pax1a and pax1b…………………………………...… 38 3.2 pax1a and pax1b are co-expressed in pharyngeal pouches…….. 38 3.3 Tg(pax1b: eGFP) enhancer trap fish line expressing eGFP in the developing pharyngeal pouches………………………………… 40 3.4 Deficiency of pax1a and pax1b leads to defects in the development of ceratobranchial cartilage and reduced expression of dlx2a and hand2…………………………………………………………..... 42 3.5 Evaluation of apoptosis and cell proliferation rate in pax1a and pax1b deficiency embryos…………………………………….... 46 3.6 Deficiency of pax1a and pax1b leads to defects in pharyngeal pouch morphogenesis and absence of endodermal expression of fgf3, tbx1 and edn1 in pharyngeal pouches…………………………...…... 47 3.7 Pax1a and Pax1b regulate ceratobranchial cartilage formation by modulating expression of fgf3, tbx1 and edn1………….…….… 48 4. Dissussion…………………………………………………….…...... 52 5. Conclusion…………………………………………………….….… 59 Reference………………………………………………………..…..… 60 Figures…………………………………………………………………. 67 Fig. 1. Embryonic expression level of pax1a-201 and pax1a-202…. 67 Fig. 2. Developmental expression patterns of zebrafish pax1a gene. 68 Fig. 3. Developmental expression patterns of zebrafish pax1b gene. 70 Fig. 4. pax1b is expressed in pharyngeal pouches but not in the neural crest cells of pharyngeal arches……………………………… 72 Fig. 5. Comparison of pax1b expression level between wild type and Tg (pax1b: eGFP) embryos…………………………..………….. 73 Fig. 6. Time lapse analyses of Tg(pax1b:eGFP) enhancer trap transgenic embryos reveals sequential segmental development of pharyngeal pouches…………………………………………….………… 74 Fig. 7. Evaluation of specificity of pax1a MO and pax1b MO…..… 75 Fig. 8. Pax1a and Pax1b have redundant function in regulating pharyngeal cartilage development…………………………… 77 Fig. 9. pax1a- and pax1b-deficient embryos generated by CRISPR-Cas9 mutagenesis exhibit abnormality of hyoid cartilage and lack of ceratobranchial cartilages 1-4.……………..………………… 78 Fig. 10. Knockdown of pax1a and pax1b decreases expression of neural crest markers dlx2a or hand2 or endodermal pouches marker nkx2.3……………………………………………………….... 80 Fig. 11. pax1a- and pax1b-deficient embryos display decrease or absence of dlx2a and hand2 expression in pharyngeal arches. 81 Fig. 12. Reduced dlx2a expression in pharyngeal arches 3-4 in pax1a; pax1b morphants beginning at 22 hpf…………..…………… 82 Fig. 13. Similar levels of apoptosis and proliferation were observed in pax1a- and pax1b-deficient embryos……………….……….. 83 Fig. 14. pax1a- and pax1b-deficient embryos exhibit morphogenetic defects and lack of nkx2.3 or Alcama expression in the pharyngeal pouches…………………………………………………….… 85 Fig. 15. Reduced expressions of edn1, fgf3, or tbx1 in pharyngeal pouches are detected in pax1a; pax1b morphant embryos..…. 86 Fig. 16. pax1a- and pax1b-deficient embryos exhibit absent fgf3, tbx1 and edn1 expression in pharyngeal pouches……………….… 88 Fig. 17. Pax1b directly binds to PAX binding elements 5′ upstream of the fgf3 gene..………………………………………………… 89 Fig. 18. Western blot for Pax1 and Myc-Pax1 expression…...…….. 90 Fig. 19. A proposed model describing the function of Pax1a and Pax1b in regulating pharyngeal pouch morphogenesis and ceratobranchial cartilage formation……………………….…. 91 Publication list……………………………………………………...…. 92 | |
dc.language.iso | en | |
dc.title | 斑馬魚Pax1a和Pax1b調控咽囊形態形成與角鰓軟骨發育之研究 | zh_TW |
dc.title | Function of Zebrafish Pax1a and Pax1b in pharyngeal pouch morphogenesis and ceratobranchial cartilage development | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 胡清華(Chin-Hwa Hu),李士傑(Shyh-Jye Lee),管永恕(Yung-Shu Kuan),王文德(Wen-Der Wang) | |
dc.subject.keyword | Pax1a蛋白質,Pax1b蛋白質,斑馬魚,咽弓,咽囊,fgf3基因,tbx1基因, | zh_TW |
dc.subject.keyword | Pax1a,Pax1b,Zebrafish,Pharyngeal pouch,Pharyngeal arch,fgf3,tbx1, | en |
dc.relation.page | 92 | |
dc.identifier.doi | 10.6342/NTU202000783 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2020-04-29 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-109-1.pdf | 4.54 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。