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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 繆希椿(Shi-Chuen Miaw) | |
| dc.contributor.author | Yi-Han Ge | en |
| dc.contributor.author | 葛奕含 | zh_TW |
| dc.date.accessioned | 2021-06-17T04:57:27Z | - |
| dc.date.available | 2021-08-01 | |
| dc.date.copyright | 2018-08-01 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-07-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71185 | - |
| dc.description.abstract | Macrophages are a type of immune cells that have the ability to engulf and digest foreign substances by phagocytosis, antigen presentation, anti-microbial activity and wound healing. Furthermore, macrophages play an important role in innate immunity and assist initiation of adaptive immunity by recruiting other immune cells such as dendritic cells and lymphocytes. There are two major subsets of macrophages, M1 and M2, which have distinct functions and features. Recent studies indicated that metabolism is important for regulating macrophage function and phenotype. However, whether the cellular metabolism in macrophage affects the differentiation of M1 and M2 remains unclear. Therefore, the aim of my research is to investigate the role of metabolic genes in macrophage differentiation. First, I performed macrophage development from bone marrow (BM) cells and immortalized bone marrow (imBM) cell line in vitro, and I showed BM cells and imBM cell line can develop into macrophages with L929 culture supernatant containing M-CSF. Next, I characterized the differentiation of macrophage from BM cells and imBM cell line in vitro. Bone marrow-derived macrophage (BMDM) and immortalized bone marrow cell line macrophage (imBMM) can differentiated into M1 and M2 subsets stimulated by LPS/IFN- and IL-4/IL-13, respectively. Further, we employed bioinformatics analysis assay to select the candidate metabolic genes expressed more than two-fold difference between M1/M2 in both human and mice. Next, I have confirmed these candidate genes expression in the subsets of macrophages derived from BMDMs and imBMMs. We knockdowned these candidate metabolic genes in imBMs by shRNA and identified some candidate metabolic genes could affect macrophage differentiation. We’ll confirm these results in BM cells in vitro. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T04:57:27Z (GMT). No. of bitstreams: 1 ntu-107-R05449006-1.pdf: 8919425 bytes, checksum: 3deb0b619e3c8a8472d8d053f1b3e4c2 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 致謝..............................................i
中文摘要..........................................ii Abstract..........................................iii Contents..........................................v Figures of content................................viii Tables of content.................................ix I. Introduction...................................1 1. An overview of macrophages.....................1 2. Macrophage subsets.............................1 2.1 M1 markers：Socs3 and Nos2....................2 2.2 M2 markers：Arg1 and Egr2.....................2 3. An overview of metabolism in macrophages.......3 4. Hematopoietic progenitor cell line.............4 5. Candidate metabolic genes......................4 6. Significance and Specific Aims.................5 6.1 Specific aim 1:...............................5 6.2 Specific aim 2:...............................5 6.3 Specific aim 3:...............................6 II. Materials and Methods.........................7 1. Materials......................................7 2. Methods.......................................10 2.1 Isolation and development of bone marrow-derived macrophage (BMDMs)...............................10 2.2 Culture of the conditionally immortalize early hematopoietic progenitor cell line...............11 2.3 In vitro differentiation of M1 and M2 subsets.12 2.4 Characterization of M1 and M2 macrophage by flow cytometry.........................................12 2.5 Characterization of M1 and M2 macrophage by real-time PCR...............................................13 2.6 Confirmation of candidate metabolic genes expressed in the subsets of macrophages by real-time PCR....13 2.7 To knockdown candidate metabolic genes in imBMs by shRNA.............................................14 III. Results......................................16 1. Both bone marrow (BM) cells and immortalized bone marrow (imBM) cells are able to develop into macrophages in vitro..........................................16 2. Both BMDM and imBMM can differentiate into M1 and M2 subsets...........................................17 3. BMDM- and imBMM- differentiated M1 and M2 cells express related genes.............................17 4. Candidate metabolic genes expressed in the subsets of macrophages differentiated from BMDMs and imBMMs is consistent with expected data by bioinformatics analysis..........................................18 5. Investigation of M1/M2-related markers expression in imBMM-derived M1/M2 macrophages by knockdown approach..........................................18 IV. Discussion....................................21 V. Figures........................................24 VI. Tables........................................44 VII. References...................................60 | |
| dc.language.iso | en | |
| dc.title | 探討代謝基因在巨噬細胞分化所扮演的角色 | zh_TW |
| dc.title | Investigating the role of metabolic genes in macrophage
differentiation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張智芬(Zee-Fen Chang),李建國(Chien-Kuo Lee) | |
| dc.subject.keyword | 巨噬細胞,代謝,巨噬細胞分化, | zh_TW |
| dc.subject.keyword | macrophage,metabolism,macrophage differentiation, | en |
| dc.relation.page | 70 | |
| dc.identifier.doi | 10.6342/NTU201802002 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-07-27 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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| ntu-107-1.pdf 目前未授權公開取用 | 8.71 MB | Adobe PDF |
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