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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張雅珮 | |
dc.contributor.author | Yen-Wen Liu | en |
dc.contributor.author | 劉晏文 | zh_TW |
dc.date.accessioned | 2021-06-17T04:57:06Z | - |
dc.date.available | 2018-08-01 | |
dc.date.copyright | 2018-08-01 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-07-27 | |
dc.identifier.citation | 邱柏喻. (2013). 硫酸乙醯肝素酶對小鼠骨癒合之影響. (碩士), 國立臺灣大學, 台 北市.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71178 | - |
dc.description.abstract | 骨癒合向來是熱門的研究議題,範圍涵蓋臨床上功能與巨觀的解剖構造的恢復以及細胞分子層面的變化。硫酸乙醯醣蛋白(Heparin sulfate proteoglycans, HSPGs)是普遍存在於生物體細胞表面和細胞外基質中的醣蛋白,參與許多生長因子的訊號傳遞。第三型硫酸乙醯肝素酶(heparinase III)是一種可以切割HSPGs上硫酸乙醯肝素(heparin sulfate, HS)的酵素,對於HSPG和其所調節的分子訊號有間接的影響。本實驗的目的為評估heparinase III作用於HS後對小鼠骨缺損癒合的影響。本實驗中小鼠為實驗動物模式,在其遠端股骨製造一直徑1 mm之圓柱狀骨缺損,利用連續給藥膠囊及導管分別將heparinase III和heparinase III緩衝液給到實驗組和控制組的骨缺損處,並在術後第1、4、7、14、21天犧牲小鼠,以微電腦斷層、組織學檢查、定量聚合酶鏈式反應、和免疫化學染色的方式評估癒合情況。實驗組和控制組間最顯著的差別在術後第1天的蝕骨細胞acid phosphatase化學染色結果以及術後第4天蝕骨細胞的Cd68基因表現量。Heparinase III處理的組別在上述的兩種評估中皆表現出顯著較高的蝕骨細胞數量與分佈。由本實驗的結果可以得知heparinase III不會影響骨癒合最終的形態結果,但在早期蝕骨細胞的聚集和骨重吸收的作用中應扮演著重要的角色。 | zh_TW |
dc.description.abstract | The healing of bone tissuehas always been a popular researchtopic. Besides restorationof clinical functionand returning to a macroscopic normal anatomical structure, changes at the microscopic cellular and molecular level should also be investigated. Heparin sulfate proteoglycans(HSPGs) are found on cell surfaceand in extracellularmatrix, where they participatein many signaling pathways of different growth factors. It is postulated thatmodulating HSPGs may influencethe processof bone healing indirectly.Heparinase III derived from microorganismiscapable of cleavingthe heparansulfate (HS) chain on HSPGs. This study aims to evaluate the effectof heparinase III in bone healing in mouse model. Mice with a distal femur bone defect were used as the animal model. Mice were randomly assigned to either the control group receiving heparinase buffer or the experiment group receiving heparinase III in heparinase buffer. The mice were sacrificed at1, 4, 7, 14, 21 days post-injury, and micro-CT, histological exam, qPCR, and immunohistochemistrywere performedto evaluate bone healing. Results showedthat the main difference betweenthe two groups are acid phosphatase histochemistry results at 1 day post-injury and Cd68 qPCR results at 4 days post-injury, with heparinase III group showing significant higher osteoclastnumbers and distribution. These results indicate that heparinase III may not affectthe final outcomeof bone healing, but certainly play a role in the early stage of osteoclastrecruitmentand bone resorption. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:57:06Z (GMT). No. of bitstreams: 1 ntu-107-R04643007-1.pdf: 67216151 bytes, checksum: b8e601688f65d4d3a39fbcba2cc9386d (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 I 中文摘要 II ABSTRACT III CONTENTS IV 圖次 VIII 表次 XI 第1章、 緒論 1 1.1 前言 1 1.2 研究動機 1 第2章、 文獻檢討 3 2.1 骨折癒合 3 2.1.1 骨化方式 3 2.1.2 骨癒合的生理 4 2.1.2.1 急性炎症反應 5 2.1.2.2 間質幹細胞聚集 5 2.1.2.3 軟骨痂和骨周邊硬骨痂的生成 5 2.1.2.4 血管新生及血管重建 6 2.1.2.5 軟骨痂的礦物化和重吸收 6 2.1.2.6 骨重塑 7 2.2 骨生成之相關機制與分子訊號 8 2.2.1 軟骨分化 8 2.2.2 生長板發育 9 2.2.3 造骨細胞生成 11 2.3 骨癒合的評估 13 2.4 硫酸乙醯肝素蛋白聚糖 14 2.4.1 結構及特性 14 2.4.2 功能 17 2.4.2.1 組織基底膜屏障 18 2.4.2.2 細胞訊號的傳遞與型態生成 19 2.4.2.3 組織的受傷與修復 19 2.4.2.4 HSPG的負面影響 20 2.4.3 硫酸乙醯肝素酶 21 第3章、 試驗研究 25 3.1 目的 25 3.2 前導實驗 26 3.3 材料與方法 30 3.3.1 實驗設計 30 3.3.2 實驗動物 30 3.3.3 植入式連續給藥膠囊 31 3.3.4 手術步驟 32 3.3.5 實驗動物之安樂死 33 3.3.6 骨缺損癒合程度的評估 33 3.3.6.1 微電腦斷層影像檢查 34 3.3.6.2 組織學檢查 35 3.3.6.3 定量聚合酶鏈式反應 (qPCR) 36 3.3.6.4 免疫化學染色 37 3.3.7 統計分析 38 第4章、 結果 39 4.1 影像學量化統計 39 4.2 組織學檢查結果 44 4.3 定量聚合酶鏈式反應結果 51 4.4 免疫化學染色結果 59 4.4.1 TRAP化學染色 59 4.4.2 Osteocalcin免疫化學染色 60 第5章、 討論 73 5.1 骨缺損癒合時的形態變化 73 5.2 骨缺損癒合時細胞分子層面之變化 75 5.3 HEPARINASE III對骨癒合的影響 78 5.4 實驗設計之限制 79 第6章、 結論 81 REFERENCES 82 | |
dc.language.iso | zh-TW | |
dc.title | 第三型硫酸乙醯肝素酶對小鼠骨癒合的影響 | zh_TW |
dc.title | The Effect of Heparinase III in Bone Healing in Mouse Model | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 劉逸軒 | |
dc.contributor.oralexamcommittee | 武敬和,皇甫維君,梁碧惠 | |
dc.subject.keyword | 硫酸乙醯醣蛋白,第三型硫酸乙醯肝素梅,硫酸乙醯肝素,骨缺損,骨癒合, | zh_TW |
dc.subject.keyword | Heparin sulfate proteoglycans,Heparinase III,heparan sulfate,bone healing,bone defect, | en |
dc.relation.page | 89 | |
dc.identifier.doi | 10.6342/NTU201802008 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-07-27 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床動物醫學研究所 | zh_TW |
顯示於系所單位: | 臨床動物醫學研究所 |
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