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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林乃君(Nai-Chun Lin) | |
dc.contributor.author | Ming-Chu Huang | en |
dc.contributor.author | 黃明珠 | zh_TW |
dc.date.accessioned | 2021-06-17T04:51:36Z | - |
dc.date.available | 2028-07-30 | |
dc.date.copyright | 2018-08-01 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-07-30 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71073 | - |
dc.description.abstract | 草莓為薔薇科草本植物,為臺灣重要經濟作物,雖為宿根性植物,但在臺灣栽培時由於病蟲害嚴重且不易越夏,以至於需要年年更新母株以培育健康子苗。目前農民仍多採用自行留種的方式,自田間選取健康無病徵之植株留作下一季育苗母株使用,然而由於炭疽病菌 (Colletotrichum gloeosporioides) 潛伏感染的特性及萎凋病菌 (Fusarium oxysporum f. sp. fragariae) 會經由走莖傳播至子株等問題,如何確定母株不帶特定病原而能培育出健康種苗,成為目前在草莓育苗期亟需克服的難題。因此本研究旨在篩選草莓根部有益內生細菌,透過生物健化改善草莓組織培養苗之特性,使其能夠快速生長並具抗病性,將有助於縮短其馴化時程,並提升草莓植株對炭疽病之抗性。未來希望能將之導入草莓育苗期,協助落實以草莓種苗三級繁殖制度培育健康種苗之政策。自健康草莓根系分離出之內生細菌,首先透過培養基測試其溶磷、嵌鐵及固氮等可促進植物生長之活性,再進一步進行促進草莓營養生長試驗與提升草莓抗炭疽病能力試驗。結果顯示,生物健化 30 天後可有效提升草莓組織培養苗營養生長及抗生物性逆境能力。具促進草莓生長潛力之五株菌株中,以 Pseudomonas putida L5 效果與對照組相較下具有顯著性差異,並與前人證實可提升草莓營養生長及對生物逆境耐受性的印度梨型孢菌 (Serendipita indica) 混合施用,具有些微加乘作用,並可加速跳苗之速度,以便快速並穩定地產生大量子苗;接種後能提升草莓組織培養苗對炭疽病抗性之菌株則有四株,且本研究所篩選之內生細菌效果皆優於 S. indica,其中又以 P. fluorescens K1-3-1 效果最為顯著。此外,P. fluorescens K1-3-1 在對峙培養試驗亦可有效直接抑制炭疽病菌及萎凋病菌菌絲生長。將 P. putida L5 與 S. indica 混合施用在促進草莓營養生長能力方面極為穩定,且在提升炭疽病抗性亦有不錯的效果為本研究所得混合菌株之最佳組合。 | zh_TW |
dc.description.abstract | Strawberry (Fragaria x ananassa Duchesne) is an economically important herbaceous plant belonging to family Rosaceae. Although strawberry is a perennial plant, replacement of mother plants for breeding healthy seedlings is required annually due to severe pest problems and difficulties of strawberry plants to survive over summer. Farmers are still preserving the mother plants by selecting healthy and symptomless plants from fields as the mother plants for next season. Due to latent infection of Colletotrichum gloeosporioides and infection of daughter plants by Fusarium oxysporum. f. sp. fragariae through runners, to ensure whether the mother plants are specific-pathogens-free (SPF) becomes an important issue in the strawberry nurseries. The purpose of this study was to screen beneficial endophytic bacteria from strawberry roots for biohardening of the micropropagated strawberry seedlings, making them capable of growing fast and resistant to anthracnose, and shortening acclimatization time. Hopefully, these endophytic bacteria can be introduced into the three-tier seedling production system in the near future. Endophytic bacteria isolated from the healthy strawberry roots were firstly tested for their potential in promoting plant growth activities, including phosphate solubilization, siderophore production, and nitrogen-fixation, through plate assay methods. The micropropagated strawberry seedlings after biohardening for 30 days with five endophytic bacteria, among which Pseudomonas putida L5 has the best activities, could effectively increase vegetative growth and resistance to anthracnose. Biohardening with P. putida L5 in combination with Serendipita indica, the endophytic fungus which also has the ability to enhance strawberry growth and resistance to anthracnose, could accelerate the seedling raising process, and produce abundant seedlings quickly and stably. Furthermore, four strains were selected on its ability to enhance resistance of micropropagated strawberry seedlings to anthracnose, all of which have activities superior to S. indica. Among those strains, P. fluorescens K1-3-1 has the best performance. In addition, P. fluorescens K1-3-1 could also inhibit mycelial growth of C. gloeosporioides and F. o. f. sp. fragariae in an antagonistic assay. At last, biohardening with P. putida L5 and S. indica, the best choice among all different combinations, created an excellent effect on promoting vegetative growth of micropropagated strawberry seedlings as well as increasing resistance against anthracnose at the same time. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:51:36Z (GMT). No. of bitstreams: 1 ntu-107-R05645003-1.pdf: 4020289 bytes, checksum: 117c1ea9c2afa52626659fd13173de5d (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 摘要i
Abstractii 目錄iv 表目錄ix 圖目錄x 附錄目錄xi 壹、前人研究1 一、草莓基本特性1 二、台灣草莓栽培模式2 三、台灣草莓育苗期重要病害3 1.炭疽病 (Anthracnose)3 2.萎凋病 (Fusarium wilt)4 3.果腐病 (Fruit rot)5 4.灰黴病 (Gray mold rot)5 5.白粉病 (Powdery mildew)5 6.青枯病 (Bacterial wilt)5 7.葉芽線蟲 (Foliar or leaf nematode)5 四、土壤微生物 (Soil microorganisms)5 1.促進植物生長有益微生物 Plant growth-promoting microorganisms (PGPMs)6 2.內生菌 (Endophytes)8 五、印度梨型孢菌 (Serendipita indica)13 六、生物健化 (Biohardening)16 貳、研究動機與目的20 參、材料與方法22 一、供試植物與菌株22 二、內生細菌之分離與鑑定22 1.草莓根系內生細菌之分離22 2.內生細菌之分子鑑定22 三、內生細菌之特性分析25 1.促進植物生長能力測試25 2.拮抗病原菌能力測試27 3.內生細菌對化學藥劑之感受性28 4.內生細菌親和性試驗28 四、內生細菌及Serendipita inidica 接種源製備29 1.內生細菌之活化29 2.內生細菌接種試驗29 3.S. inidica之活化29 4.S. inidica 接種試驗30 5.內生細菌與S. inidica 混合接種試驗30 五、內生細菌及S. inidica於草莓根系定殖狀況30 1.內生細菌於草莓組培苗根系定殖狀況30 2.轉殖綠色螢光蛋白至內生細菌31 3.S. inidica於草莓組織培養苗根系定殖狀況31 六、接種內生細菌及 S. inidica 對草莓組織培養苗出瓶健化及營養生長促進之探討32 七、接種內生細菌及 S. inidica 對草莓組織培養苗抗炭疽病能力影響之探討33 1.製備草莓炭疽病菌孢子懸浮液33 2.炭疽病菌接種試驗33 八、統計分析34 肆、結果36 一、草莓內生細菌之純化與分離36 二、接種內生細菌對促進草莓組織培養苗營養生長之影響36 1.篩選可促進草莓組織培養苗生長潛力之內生細菌36 2.接種具促進草莓生長潛力內生細菌對草莓組織培養苗生長指標之影響37 3.潛力細菌菌株於草莓植株內部定殖情形39 4.具促進草莓生長潛力內生細菌進行生物健化 60 天後對草莓組織培養苗生長相關指標之影響40 5.經馴化 30 天後再以土壤澆灌菌液方式處理對草莓組織培養苗生長相關指標之影響41 三、內生細菌與 Serendipita indica 混合施用之效果評估41 四、接種內生細菌對草莓組織培養苗抗草莓炭疽病能力之影響44 1.探討可提升草莓組織培養苗對炭疽病抗性之內生細菌對植物生長之影響44 2.內生細菌與特定病原之對峙培養試驗45 五、內生細菌與 Serendipita indica 混合施用對草莓組織培養苗抗炭疽病能力之影響46 1.可提升草莓組織培養苗對炭疽病抗性之內生細菌與 Serendipita indica 個別與混合施用對提升草莓組織培養苗抗炭疽病能力之比較46 2.可提升草莓組織培養苗對炭疽病抗性之內生細菌對草莓生長之影響46 3.本研究篩選出具促進生長潛力內生細菌與 Serendipita indica 混合施用對草莓組織培養苗抗炭疽病能力之影響47 4.具促進生長潛力內生細菌與 S. indica 混合施用於草莓組織培養苗後,S. indica 於草莓根系之定殖率47 六、經 Serendipita indica 進行生物健化之第二代走莖苗葉片對炭疽病之抗性評估48 七、內生細菌鑑定與特性分析48 1.菌種鑑定48 2.菌株親和性試驗48 3.本研究篩選出之內生細菌對草莓栽培中常用化學藥劑之感受性49 伍、討論50 一、草莓內生細菌之純化與分離50 1.內生細菌於培養基促進生長特性之測試50 2.潛力細菌菌株於草莓植株內部定殖情形51 二、內生細菌與 Serendipita indica 混合施用之效果評估52 1.篩選可促進草莓組織培養苗生長潛力之內生細菌53 2.具促進草莓生長潛力內生細菌進行生物健化 60 天後對草莓組織培養苗生長相關指標之影響55 3.經馴化 30 天後再以土壤澆灌菌液方式處理對草莓組織培養苗生長相關指標之影響56 三、接種內生細菌及 S. indica 草莓組織培養苗對草莓炭疽病抗性之探討57 1.篩選可提升草莓組織培養苗對炭疽病抗性之內生細菌57 2.可提升草莓組織培養苗對炭疽病抗性之內生細菌對植物生長之影響58 3.本研究篩選出具促進生長潛力內生細菌與 Serendipita indica 混合施用於草莓組織培養苗後對炭疽病抗性之影響58 四、內生細菌特性分析59 1.菌株親和性試驗59 2.本研究篩選出之內生細菌對草莓栽培中常用化學藥劑之感受性60 五、Serendipita indica 生物健化苗之初步田間試驗60 陸、未來展望62 柒、參考文獻63 捌、表87 玖、圖97 拾、附錄114 | |
dc.language.iso | zh-TW | |
dc.title | 接種內生菌對草莓組織培養苗生物健化之影響評估 | zh_TW |
dc.title | Investigation of the effects of the endophytic microorgamisms on biohardening of micropropagated strawberry seedlings | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾嘉綾(Chia-Lin Chung),陳昭瑩(Chao-Ying Chen),洪挺軒(Ting-Hsuan Hung),葉信宏(Hsin-Hung Yeh) | |
dc.subject.keyword | 草莓,內生菌,生物健化,草莓炭疽病,假單胞菌屬, | zh_TW |
dc.subject.keyword | strawberry,endophytes,biohardening,strawberry anthracnose,Pseudomonas spp., | en |
dc.relation.page | 125 | |
dc.identifier.doi | 10.6342/NTU201703754 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-07-31 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
顯示於系所單位: | 植物醫學碩士學位學程 |
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