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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林讚標 | |
| dc.contributor.author | Jui-Hung Chen | en |
| dc.contributor.author | 陳瑞宏 | zh_TW |
| dc.date.accessioned | 2021-05-17T10:18:14Z | - |
| dc.date.available | 2012-03-19 | |
| dc.date.available | 2021-05-17T10:18:14Z | - |
| dc.date.copyright | 2012-03-19 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2012-02-09 | |
| dc.identifier.citation | Bai L, Zhang G, Zhou Y, Zhang Z, Wang W, Du Y, Wu Z, Song C-P (2009) Plasma membrane-associated proline-rich extensin-like receptor kinase 4, a novel regulator of Ca2+ signalling, is required for abscisic acid responses in Arabidopsis thaliana. Plant J 60: 314–327.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7080 | - |
| dc.description.abstract | 麩胺基硫轉移酶 (glutathione s-transferases, GSTs) 在氧化逆境代謝中扮演一個相當重要的角色，但對於這類基因在植物體內所扮演的個別功能卻所知有限。在整個GST基因家族中GLUTATHIONE S-TRANSFERASE U17 (AtGSTU17, At1g10370) 曾有被報導其參與了光的傳導訊息調控；經由與phyA的交互作用，影響了植物體內的GSH含量，進一步影響了植物的生長發育。
本篇論文提供進一步的研究證據顯示AtGSTU17在乾旱及鹽分逆境下扮演一個重要的負調控角色。阿拉伯芥的atgstu17突變株比野生型更為耐旱及耐鹽。生理分析顯示atgstu17突變株的植物體累積了較高含量的GSH與ABA，同時在發芽時期對於ABA較不敏感，葉片的氣孔孔徑較小，較低的水分蒸發速率，根系發育更為茂盛以及較長的營養生長期等生理性狀。 為了釐清atgstu17突變株累積的ABA是否是因為GSH含量升高引起，我們對野生型澆灌GSH溶液進行研究。結果發現澆灌GSH溶液的野生型，其ABA含量較未澆灌GSH的植株高，同時顯現atgstu17突變株的生理性狀，如開花延遲，根部發育，較為耐鹽與耐旱等。進一步研究atgstu17突變株是否是因為GSH與ABA累積影響而產生了上述的生理性狀。我們將atgstu17突變株種植在L-buthionine-(S,R)-sulfoximine (BSO)溶液中。BSO是一種可以抑制植物的GSH生合成的專一性藥劑。當atgstu17突變株的GSH含量受到BSO抑制減少到與野生型相同的含量時，觀察其性狀與生理反應，如根系，開花時間與對於鹽分與乾旱的耐受性等，顯示與野生型類似。由以上實驗結果可以得到一個結論，atgstu17突變株的外表性狀是由於其植物體內含有較高的GSH與ABA所共同作用而產生的結果。同時經由DNA微陣列 (microarray) 的資料顯示許多與生長或逆境相關的轉錄調控基因受到AtGSTU17的影響而改變表現。綜合以上實驗資料結果，我們推測AtGSTU17扮演了植物逆境訊息傳導反應的負調控功能。 | zh_TW |
| dc.description.abstract | Although glutathione S-transferases (GSTs) are thought to play major roles in oxidative stress metabolism, little is known about the regulatory functions of GSTs. We have reported that GLUTATHIONE S-TRANSFERASE U17 (AtGSTU17, At1g10370) participates in light signaling and might modulate various aspects of development by affecting glutathione (GSH) pools via a coordinated regulation with phyA. Here we provided further evidence to support a negative role of AtGSTU17 in drought and salt stress tolerance.
When AtGSTU17 was mutated, plants were more tolerant to drought and salt stresses compared to wild-type (WT, Col-0) plants. In addition, atgstu17 accumulated higher level of GSH and abscisic acid (ABA), and exhibited hyposensitivity to ABA during seed germination, smaller stomatal apertures, a lower transpiration rate, better development of primary and lateral root systems, and longer vegetative growth. To explore how atgstu17 accumulated higher ABA content, we grew WT in the solution containing GSH and found that plants accumulated ABA to a higher extent than plants grown in the absence of GSH, and exhibited the atgstu17 phenotypes. WT plants treated with GSH also demonstrated more tolerant to drought and salt stresses. Furthermore, the effect of GSH on root patterning and drought tolerance was confirmed by growing the atgstu17 in solution containing L-buthionine-(S,R)- sulfoximine (BSO), a specific inhibitor of GSH biosynthesis. In conclusion, the atgstu17 phenotype can be explained by the combined effect of GSH and ABA. Microarray analysis provided evidence that expressions of many genes related to growth and stress inducible transcription factors altered in the atgstu17 mutants. We propose a role of AtGSTU17 in adaptive responses to drought and salt stresses, by functioning as a negative component of stress-mediated signal transduction pathways. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-17T10:18:14Z (GMT). No. of bitstreams: 1 ntu-100-D92b42005-1.pdf: 5294550 bytes, checksum: f756918ac2631df69ecac8a1f6fb288c (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 中文摘要 I
ABSTRACT II CONTENTS IV INTRODUCTION 1 MATERIALS AND METHODS 5 Plant Material and Growth Conditions 5 Stress-tolerance Tests, Water Loss Measurement and Feeding Experiment 5 Seed Germination and Stomatal Aperture Measurements 6 Histochemical GUS Assay 7 Subcellular Localization 7 Quantification of the GSH and ABA Content 8 RNA Isolation and Quantitative Real-time (q)RT-PCR Analysis 9 Microarray Analysis 9 RESULTS 11 AtGSTU17 Gene in Arabidopsis thaliana 12 AtGSTU17 Affects Arabidopsis Developments 12 Tolerance to Drought and Salt Stresses of the atgstu17 Mutants 13 Effect of Abiotic Stresses on AtGSTU17 Gene Expression. 13 Tissue-specific expression of the AtGSTU17 protein 14 The atgstu17 Mutants Exhibit a Reduced Water Loss and Smaller Stomatal Aperture 14 The atgstu17 Mutants Show Altered Physiological Responses Regulated by ABA 15 The atgstu17 Has Higher GSH and ABA Contents Compared to WT Plant 16 Exogenous GSH Induced ABA Accumulation in Planta 17 Effects of Exogenous GSH and ABA on Seed Germination, Stomata Aperture Size, Root architecture and Stress Tolerances 17 atgstu17 Phenotypes were Abolished by GSH Synthesis Inhibitor 18 Effects of GSH and ABA on Stress Tolerances 19 Global Gene Expression in AtGSTU17-Knockout Plants Identified by a GeneChip Analysis 20 Expression of Selected Genes from the Microarray Dataset in the AtGSTU17 Mutant Lines and WT Plants 22 AtGSTU17 Regulation of ABA-Downstream and ABA-independent Gene Expressions under Dehydration Conditions 22 AtGSTU17 Induced by ABA-Dependent and -Independent Pathways under Drought Stress Treatment 23 DISCUSSION 25 atgstu17 plants accumulated higher level of GSH in shoot and root 26 AtGSTU17 Plays a Negative Role in Drought and Salt Stress Tolerance 26 The Role of GSH and ABA in Enhancing Drought and Salt Tolerance 27 GSH’s Effects on the Stomata Aperture Size and Root Patterning 29 REFERENCE 31 TABLES 39 FIGURES 50 APPDENDIX 83 | |
| dc.language.iso | en | |
| dc.title | 阿拉伯芥麩胺基硫轉移酶 AtGSTU17 突變株耐旱及耐鹽性狀為麩胺基與離層酸共同作用之結果 | zh_TW |
| dc.title | Drought and Salt Stress Tolerance of Arabidopsis Glutathione S-Transferase U17 Knockout Mutant are Attributed to the Combined Effect of Glutathione and Abscisic Acid | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 鄭石通,謝旭亮,洪傳揚,陳虹樺,葉國楨 | |
| dc.subject.keyword | 麩胺基硫轉移?U17,離層酸訊息傳導,耐旱,榖胱甘肽,側根發育,耐鹽, | zh_TW |
| dc.subject.keyword | glutathione S-transferase U17,ABA signaling,drought tolerance,glutathione,lateral root development,salt tolerance, | en |
| dc.relation.page | 90 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2012-02-09 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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