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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 蕭信宏 | |
dc.contributor.author | Yu-Chieh Hsu | en |
dc.contributor.author | 徐瑀婕 | zh_TW |
dc.date.accessioned | 2021-06-17T04:37:51Z | - |
dc.date.available | 2023-10-03 | |
dc.date.copyright | 2018-10-03 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-07 | |
dc.identifier.citation | Anders RF, Adda CG, Foley M, and Norton RS. Recombinant protein vaccines against the asexual blood stages of Plasmodium falciparum. Hum Vaccin. 2010; 6:39-53.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70770 | - |
dc.description.abstract | 蚊子是許多疾病的傳播媒介,例如:瘧疾、登革熱、西尼羅熱、屈躬病、黃熱病以及茲卡等。當中,登革熱為熱帶地區重要的疾病之一。台灣在2014 至 2015 年間爆發嚴重的登革熱疫情,這兩年造成超過5 萬例的登革熱感染案例。然而目前卻無有效的疫苗及藥物上市。因此,如何預防病媒蚊的叮咬以及控制病媒蚊的數量為當前防治病蚊媒傳染疾病最重要的研究課題。登革熱的傳播主要是透過病媒蚊的叮咬。在叮咬的過程中,病媒蚊之唾液蛋白會隨著登革病毒一併進入到人體內。先前在哺乳類的研究中指出,蚊子唾腺中的蛋白會影響登革病毒的感染,但確切的機制為何目前尚不甚清楚。值得注意的是,並非所有唾腺中的蛋白會隨著唾液排出。因此,本研究以埃及斑蚊為研究模式,著重在探討蚊子分泌出的唾液蛋白在登革病毒感染宿主時所扮演的角色為何。我們的實驗結果顯示,登革病毒的 prM 蛋白會透過其蛋白上的醣和唾液蛋白進行交互作用,且唾液蛋白在登革病毒感染的過程中扮演相當重要的角色。更進一步,我們找出 calreticulin (CRT) 此唾液蛋白,發現其會和登革病毒 prM 蛋白進行結合。且此蛋白除了出現在唾液外,在埃及斑蚊的其他組織中也有表現,同時我們也證實 CRT 是調控登革病毒在埃及斑蚊體內複製的重要蛋白。再者,我們也發現當唾液中少了 CRT 此蛋白後,登革病毒之感染力明顯下降。本研究證實CRT 為登革病毒傳播及感染宿主時之重要唾液蛋白。透過了解唾液蛋白在登革病毒傳播時所扮演的角色,將提供未來規劃可行的方法以控制登革病毒的傳播。 | zh_TW |
dc.description.abstract | Mosquitoes are one of the fatal animals in the world and they act as vectors for several diseases, including malaria, dengue fever, West Nile fever, chikungunya and Zika. Among these diseases, dengue fever is currently one of the world’s most important tropical diseases. More than 50 thousand cases were reported in 2014 and 2015 in Taiwan. However, there is no effective dengue vaccine or drug available so far. Hence, vector control becomes an alternative strategy for dengue control. Dengue virus (DENV) is transmitted to humans by the mosquito Aedes aegypti during the blood meal. In the meantime, DENV and saliva proteins are inoculated into human skin. Previous studies have indicated that proteins from mosquito salivary gland may influence the DENV infectivity in the mammalian host. However, the exact mechanisms of saliva-mediated infectivity enhancement remain unknown largely. It is worth noting that not all the proteins in the salivary gland were secreted. Therefore, the aim of this study is to investigate the role of mosquito saliva on dengue virus infectivity in Aedes aegypti. Our results showed that mosquito saliva proteins are associated with dengue virus proteins in Aedes aegypti via glycan-protein interactions, and these interactions are crucial for dengue virus infectivity. Furthermore, we identified a saliva protein named calreticulin (CRT) to be interacted with dengue prM protein in the mosquito saliva. We found that CRT not only expressed in mosquito saliva but also other tissues. We also demonstrated that CRT is essential for DENV replication in Aedes aegypti. Furthurmore, silencing of CRT resulted in significant reduction of dengue virus infectivity in the mosquito saliva, indicating that CRT plays an important role in DENV transmission. Understanding the role of saliva proteins in DENV transmission will provide novel approaches for the development of new tools for the control of arbovirus-borne diseases transmitted by the same vector. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:37:51Z (GMT). No. of bitstreams: 1 ntu-107-R05445201-1.pdf: 3750110 bytes, checksum: 2393739a11326037cd8c2a25c094c8cc (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii 目錄 iv 圖目錄 vii 表目錄 viii 第一章 緒論 1 1.1. 病媒蚊傳播疾病 (Mosquito-borne disease) 1 1.1.1. 瘧疾 (Malaria) 3 1.1.2. 茲卡病毒感染症 (Zika virus infection) 3 1.2. 登革熱 (Dengue fever) 4 1.2.1. 登革病毒 (Dengue virus;DENV) 4 1.2.2. 臨床症狀 4 1.2.3. 台灣地區之流行病學 5 1.3. 埃及斑蚊生活史 6 1.4. 登革病毒生活史 6 1.4.1. 宿主細胞內之複製 6 1.4.2. 病媒蚊體內之複製 7 1.5. 登革病毒蛋白之醣化 8 1.6. prM、E 及 NS1 蛋白之醣化 8 1.7. 病媒蚊唾腺及唾液中之蛋白 9 1.8. 實驗動機與假說 10 第二章 實驗材料與方法 12 2.1. 實驗步驟與流程 12 2.1.1. 埃及斑蚊飼養與繼代 12 2.1.2. 細胞培養 (cell culture) 12 2.1.3. 病毒製備 13 2.1.4. 西方點墨法 (Western blotting) 13 2.1.5. 兩蛋白質交互作用試驗 (Far-western blotting) 14 2.1.6. 免疫沉澱試驗 (Immunoprecipitation; IP) 14 2.1.7. 蛋白質去醣 15 2.1.8. Carbohydrate membrane microarray 15 2.1.9. 凝集素競爭試驗 (Lectin competition assay) 15 2.1.10. 雙股RNA (double-stranded RNA, dsRNA) 製備 16 2.1.11. RNA萃取 (RNA extraction) 17 2.1.12. 反轉錄作用 (Reverse transcription, RT) 18 2.1.13. 聚合酶連鎖反應 (Polymerase chain reaction, PCR) 18 2.1.14. 即時定量聚合酶連鎖反應 (Real-time PCR, Quantitative PCR) 19 2.1.15. 埃及斑蚊之胸腔注射 (Injection) 19 2.1.16. 埃及斑蚊唾液 (saliva) 收取 20 2.1.17. 溶斑試驗 (Plaque assay) 20 2.1.18. 免疫螢光染色實驗 (Immunofluorescent assay;IFA) 20 2.1.19. Cell-based ELISA 21 2.2. 實驗試劑製備 22 第三章 結果 26 3.1. 埃及斑蚊唾液增強登革病毒之感染力 26 3.2. 登革病毒病毒蛋白與埃及斑蚊唾液蛋白間有交互作用 26 3.3. 登革病毒結構蛋白上之醣為其與埃及斑蚊唾液蛋白進行交互作用之關鍵 27 3.4. 與埃及斑蚊唾液蛋白交互作用之醣類 28 3.5. 登革病毒醣蛋白組成中之醣基 N-Acetylglucosamine (GluNAc) 為其與埃及斑蚊唾液蛋白進行交互作用之關鍵 28 3.6. 埃及斑蚊之唾液單一蛋白與登革病毒間之交互作用 29 3.7. Calreticulin 及 calnexin兩蛋白在埃及斑蚊各組織內的表現位置大量重合 29 3.8. 埃及斑蚊感染登革病毒後於唾腺位置大量表現 calreticulin 30 3.9. 登革病毒藉由其表面之醣來和唾液蛋白 calreticulin 進行交互作用 31 3.10. Calreticulin 為登革病毒在埃及斑蚊體內複製時所需之重要蛋白 32 3.11. Calreticulin 為埃及斑蚊在傳染登革病毒及茲卡病毒時之重要唾液蛋白 33 第四章 討論 35 4.1. 埃及斑蚊唾液對登革病毒感染力之影響 35 4.2. 埃及斑蚊唾液蛋白與登革病毒間之交互作用 35 4.3. Calreticulin 於埃及斑蚊體內之分布表現 37 4.4. Calreticulin 參與登革病毒於埃及斑蚊體內之複製 37 4.5. Calreticulin 對登革病毒及茲卡病毒傳染力之影響 38 附圖 41 附表 63 附錄 66 參考文獻 72 | |
dc.language.iso | zh-TW | |
dc.title | 探討埃及斑蚊唾液蛋白對登革病毒感染力之影響 | zh_TW |
dc.title | Effects of mosquito saliva proteins on dengue virus
infectivity in Aedes aegypti | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 余明俊,劉旻禕 | |
dc.subject.keyword | 埃及斑蚊,登革病毒,prM,唾液蛋白,calreticulin, | zh_TW |
dc.subject.keyword | Aedes aegypti,dengue virus,prM,saliva proteins,calreticulin, | en |
dc.relation.page | 80 | |
dc.identifier.doi | 10.6342/NTU201802668 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-08 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-107-1.pdf 目前未授權公開取用 | 3.66 MB | Adobe PDF |
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