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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐濟泰 | |
dc.contributor.author | Zhi-Hong Liu | en |
dc.contributor.author | 劉智宏 | zh_TW |
dc.date.accessioned | 2021-06-17T04:37:36Z | - |
dc.date.available | 2018-08-13 | |
dc.date.copyright | 2018-08-13 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-08 | |
dc.identifier.citation | 行政院農業委員會畜產試驗所。2011。臺灣飼料成分手冊,第三版。行政院農業委員會畜產試驗所,台南市。
行政院農業委員會。2015。自製自用飼料限量標準。行政院農業委員會農牧字第1040043021A號令。 行政院農業委員會。2016。統計與出版品農產品生產量值統計。 林茂勇與呂鋒洲。1991。黴菌毒素學。淑馨出版社。台北市。 許福星。2008。畜牧要覽.草食家畜篇。中國畜牧學會。台北市。 衛生福利部食品藥物管理署。2013。部授食字第1021350146號令。 Allen, L., J. Harrison, S. Watson, and W. Ferguson. 1937. A study of the chemical and bacteriological changes occurring in grass silage. The Journal of Agricultural Science 27: 271-293. Battacone, G. et al. 2005. Transfer of aflatoxin B1 from feed to milk and from milk to curd and whey in dairy sheep fed artificially contaminated concentrates. Journal of Dairy Science 88: 3063-3069. Bennett, J. W., and M. Klich. 2003. Mycotoxins. Clinical Microbiology Reviews 16: 497-516. Bilanski, W., and S. Ghate. 1978. Determining moisture content of alfalfa using a microwave oven. Ontario Ministry of Agriculture and Food. Biomin, 2016. Biomin Mycotoxin Survey 2016 Third quarter (July to Sept, 2016) Accessed Jan. 15, 2017. Bolsen, K., G. Ashbell, and Z. Weinberg. 1996. Silage fermentation and silage additives-Review. Asian-Australasian Journal of Animal Sciences 9: 483-494. Borreani, G., E. Tabacco, R. J. Schmidt, B. J. Holmes, and R. E. Muck. 2018. Silage review: Factors affecting dry matter and quality losses in silages. Journal of dairy science 101: 3952-3979. Buxton, D. R., R. E. Muck, J. H. Harrison, G. Pahlow, R. E. Muck, F. Driehuis, S. J. W. H. O. Elferink, and S. F. Spoelstra. 2003. Microbiology of ensiling. Agronomy 42: 31-94. CAST (Council for Agricultural Science and Technology). 2003. Mycotoxins: risk in plant, animal and human systems. Task Force Report No. 139. Ames, IA. Chaney, A. L., and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry. 8 (2): 130-132. Charmley, E., H. L. Trenholm, B. K. Thompson, D. Vudathala, J. W. G. Nicholson, D. B. Prelusky, and L. L. Charmley. 1993. Influence of levels of deoxynivalenol in the diet of dairy cows on feed intake, milk production, and its composition. Journal of Dairy Science 76:3580–3587. Diaz, G. J., and H. J. Boermans. 1994. Fumonisin toxicosis in domestic animals: A review. Veterinary and Human Toxicology 36: 548-555. Diekman, M. A., and M. L. Green. 1992. Mycotoxins and reproduction in domestic livestock1. Journal of Animal Science 70: 1615-1627. Diener, U. L., R. J. Cole, T. H. Sanders, G. A. Payne, L. S. Lee, and M. A. Klich. 1987. Epidemiology of aflatoxin formation by Aspergillus flavus. Annual Review of Phytopathology 25: 249-270. Driehuis, F., S. Elferink, and S. Spoelstra. 1999. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. Journal of Applied Microbiology 87: 583-594. Driehuis, F., M. C. Spanjer, J. M. Scholten, and M. C. Te Giffel. 2008a. Occurrence of mycotoxins in feedstuffs of dairy cows and estimation of total dietary intakes. Journal of Dairy Science 91: 4261-4271. Driehuis, F., M. C. Spanjer, J. M. Scholten, and M. C. Te Giffel. 2008b. Occurrence of mycotoxins in maize, grass and wheat silage for dairy cattle in the Netherlands. Food Additives and Contaminants: Part B Surveillance 1: 41-50. Edwards, S. G., B. Barrier-Guillot, P. E. Clasen, V. Hietaniemi, and H. Pettersson. 2009. Emerging issues of HT-2 and T-2 toxins in European cereal production. World Mycotoxin Journal 2: 173-179. EFSA. 2004. Panel on Contaminants in the Food Chain. Opinion of the Scientific Panel on contaminants in the food chain related to Aflatoxin B1 as undesirable substance in animal feed. Journal of European Food Safety Authority 39: 1-27. Elferink, S. J. O., J. Krooneman, J. C. Gottschal, S. F. Spoelstra, F. Faber, and F. Driehuis. 2001. Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri. Applied and Environmental Microbiology 67: 125-132. Forristal, P., and P. O’Kiely. 2005. Update on technologies for producing and feeding silage. In: Silage Production and Utilisation: Proceedings of the XIVth International Silage Conference, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Belfast, Northern Ireland. p 82. Frisvad, J. C., and R. A. Samson. 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies in Mycology 2004: 1-173. Gallo, A., G. Giuberti, J. C. Frisvad, T. Bertuzzi, and K. F. Nielsen. 2015. Review on mycotoxin issues in ruminants: Occurrence in forages, effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects. Toxins 7: 3057-3111. García-Moraleja, A., G. Font, J. Mañes, and E. Ferrer. 2015. Analysis of mycotoxins in coffee and risk assessment in Spanish adolescents and adults. Food and Chemical Toxicology 86: 225-233. Garrett, W. N., H. Heitman, Jr., and A. N. Booth. 1968. Aflatoxin toxicity in beef cattle. Proceedings of the Society for Experimental Biology and Medicine 127: 188-190. Gourama, H., and L. Bullerman. 1995. Relationship between aflatoxin production and mold growth as measured by ergosterol and plate count. LWT-Food Science and Technology 28: 185-189. Hassan, R., S. El-Kadi, and M. Sand. 2015. Effect of some organic acids on some fungal growth and their toxins production. Int. J. Adv. Biol. 2: 1-11. Hoedtke, S., and A. Zeyner. 2011. Comparative evaluation of laboratory‐scale silages using standard glass jar silages or vacuum‐packed model silages. Journal of the Science of Food and Agriculture 91: 841-849. Hoffman, P. et al. 2011. Influence of ensiling time and inoculation on alteration of the starch-protein matrix in high-moisture corn. Journal of Dairy Science 94: 2465-2474. IARC. 2002. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. World Health Organization. Johnson, H. E., R. J. Merry, D. R. Davies, D. B. Kell, M. K. Theodorou, and G. W. Griffith. 2005. Vacuum packing: a model system for laboratory‐scale silage fermentations. Journal of Applied Microbiology, 98: 106-113. Jouany, J. P., A. Yiannikouris, and G. Bertin. 2009. Risk assessment of mycotoxins in ruminants and ruminant products. Cent. Int. Hautes Etudes Agron. Méditerr. A 85: 205-224. Kaiser, A.G. 1999. Silage additives. Department of Primar Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW. Kandler, O. and Weiss, N. 1986. Regular, Non-Sporing Gram-Positive Rods. In: Sneath, H.A., Mair, N.S., Sharpe, M.E. and Holt, J.G., Eds., Bergey’s Manual of Systematic Bacteriology, Williams and Wilkins, Baltimore, 1208-1234. Kégl, T., and A. Ványi. 1991. T-2 fusariotoxicosis in a cattle stock. Magyar Allatorvosok Lapja 46: 467-471. Keller, L. A. M. et al. 2013. Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation. Journal of Stored Products Research 52: 42-47. Kleinschmit, D., and L. Kung. 2006. A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages. Journal of Dairy Science 89: 4005-4013. Korosteleva, S. N., T. K. Smith, and H. J. Boermans. 2009. Effects of feed naturally contaminated with Fusarium mycotoxins on metabolism and immunity of dairy cows. Journal of Dairy Science 92:1585–1593. Kosicki, R., A. Błajet-Kosicka, J. Grajewski, and M. Twaruzek. 2016. Multiannual mycotoxin survey in feed materials and feedingstuffs. Animal Feed Science and Technology 215: 165-180. Krooneman, J., F. Faber, A. C. Alderkamp, S. J. H. W. Oude Elferink, F. Driehuis, I. Cleenwerck, J. Swings, J. C. Gottschal, anf M. Vancanneyt. 2002. Lactobacillus diolivorans sp. nov., a 1, 2-propanediol-degrading bacterium isolated from aerobically stable maize silage. International Journal of Systematic and Evolutionary Microbiology 52: 639-646. Kung Jr, L., Robinson, J. R., Ranjit, N. K., Chen, J. H., Golt, C. M., & Pesek, J. D. (2000). Microbial populations, fermentation end-products, and aerobic stability of corn silage treated with ammonia or a propionic acid-based preservative. Journal of Dairy Science, 83(7), 1479-1486. Kung, L. 1998. A review on silage additives and enzymes. In: Proceedings of the 59th Minneapolis Nutrition Conference. p 121-135. Kung, L., and N. Ranjit. 2001. The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. Journal of Dairy Science 84: 1149-1155. Kung, L., and R. Shaver. 2001. Interpretation and use of silage fermentation analysis reports. Focus on Forage 3: 1-5. Kurtzman, C. P., B. W. Horn, and C. W. Hesseltine. 1987. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie van Leeuwenhoek 53: 147-158. Lambert, R. J., and M. Stratford. 1999. Weak-acid preservatives: modelling microbial inhibition and response. Journal of Applied Microbiology 86: 157-164. Liu, J.X. and Guo, J. 2010. Ensiling crop residues. Zhejiang University and China National Breeding Stock Import and Export Corporation. Li, Y. et al. 2011. T-2 toxin, a trichothecene mycotoxin: Review of toxicity, metabolism, and analytical methods. Journal of Agricultural and Food Chemistry 59: 3441-3453. Li, Y., Z. Wang, R. C. Beier, J. Shen, D. D. Smet, S. De Saeger, and S. Zhang. 2011. T-2 toxin, a trichothecene mycotoxin: review of toxicity, metabolism, and analytical methods. Journal of Agricultural and Food Chemistry 59(8): 3441-3453. Lindgren, S. E., L. T. Axelsson, and R. F. McFeeters. 1990. Anaerobic L-lactate degradation by Lactobacillus plantarum. FEMS Microbiology Letters 66: 209-213. Marczuk, J., K. Obremski, K. Lutnicki, M. Gajecka, and M. Gajecki. 2012. Zearalenone and deoxynivalenol mycotoxicosis in dairy cattle herds. Polish Journal of Veterinary Sciences 15: 365-372. Mathur, S., P. D. Constable, R. M. Eppley, A. L. Waggoner, M. E. Tumbleson, and W. M. Haschek. 2001. Fumonisin B1 is hepatotoxic and nephrotoxic in milk-fed calves. Toxicological Sciences 60: 385-396. McDonald, P., A. Henderson, and S. Heron. 1991. The Biochemistry of Silage., 2nd edn. Chalcombe Publications: Bucks, UK. Mills, J., and L. Kung. 2002. The effect of delayed ensiling and application of a propionic acid-based additive on the fermentation of barley silage 1, 2. Journal of Dairy Science 85: 1969-1975. Morris, D. L. 1948. Quantitative determination of carbohydrates with dreywood's anthrone reagent. Science Washington 107: 254-255. Muck, R., and R. Pitt. 1994. Aerobic deterioration in corn silage relative to the silo face. Transactions of the ASAE 37: 735-743. Musser, S. M., M. L. Gay, E. P. Mazzola, and R. D. Plattner. 1996. Identification of a new series of fumonisins containing 3-hydroxypyridine. Journal of Natural Products 59: 970-972. Nishino, N., M. Yoshida, H. Shiota, and E. Sakaguchi. 2003. Accumulation of 1, 2‐propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. Journal of Applied Microbiology 94: 800-807. O’brien, M., P. O’kiely, P. Forristal, and H. Fuller. 2008. Fungal contamination of big‐bale grass silage on Irish farms: predominant mould and yeast species and features of bales and silage. Grass and Forage Science 63: 121-137. Odland, T., T. Cox, and J. Smith. 1941. A comparison of different crops for grass silage by the use of mason jars as miniature silos. Journal of the American Society of Agronomy 33: 304-313. Oetzel, G. R. 2007. Herd-level ketosis–diagnosis and risk factors. In: proceedings of the 40th annual conference of bovine practitioners, Vancouver, Canada Ogunade, I. M., C. Martinez-Tuppia, O.C.M. Queiroz, Y. Jiang, P. Drouin, F. Wu, D. Vyas, A. T. Adesogan. 2018. Silage review: mycotoxins in silage: occurrence, effects, prevention, and mitigation. Journal of Dairy Science 101: 4034-4059. Ohyama, Y., and S. Hara. 1975. Growth of yeasts isolated from silages on various media and its relationship to aerobic deterioration of silage. Japanese Journal of Zootechnical Science. Oldenburg, E. 1991. Mycotoxins in conserved forage. Landbauforschung Voelkenrode. Sonderheft (Germany, FR). Pahlow, G. 1991. Role of microflora in forage conservation. Landbauforschung Voelkenrode. Sonderheft (Germany, FR). Pestka, J. J. 2007. Deoxynivalenol: toxicity, mechanisms and animal health risks. Animal Feed Science and Technology 137: 283-298. Peterson, W., and E. Fred. 1919. The role of pentose-fermenting bacteria in the production of corn-silage. Journal of Biological Chemistry 41: 181-186. Rodrigues, I., and K. Naehrer. 2012. A three-year survey on the worldwide occurrence of mycotoxins in feedstuffs and feed. Toxins 4: 663-675. Schmidt, P., C. O. Novinski, D. Junges, R. Almeida, and C. M. de Souza. 2015. Concentration of mycotoxins and chemical composition of corn silage: A farm survey using infrared thermography. Journal of Dairy Science 98: 6609-6619. Scott, P. M., T. Delgado, D. B. Prelusky, H. L. Trenholm, and J. D. Miller. 1994. Determination of fumonisins in milk. Journal of Environmental Science and Health, Part B 29: 989-998. Scudamore, K. A., and C. T. Livesey. 1998. Occurrence and significance of mycotoxins in forage crops and silage: a review. Journal of the Science of Food and Agriculture 77: 1-17. Streit, E., G. Schatzmayr, P. Tassis, E. Tzika, D. Marin, I. Taranu, C. Tabuc, A. Nicolau, I. Aprodu, O. Puel, and I. P. Oswald. 2012. Current situation of mycotoxin contamination and co-occurrence in animal feed focus on Europe. Toxins 4: 788-809. Trenholm, H. L., B. K. Thompson, K. E. Martin, R. Greenhalgh, and A. J. McAllister. 1985. Ingestion of vomitoxin (deoxynivalenol)-contaminated wheat by nonlactating dairy cows. Journal of Dairy Science 68: 1000-1005. Weinberg, Z., G. Ashbell, A. Azrieli, and I. Brukental. 1993. Ensiling peas, ryegrass and wheat with additives of lactic acid bacteria (LAB) and cell wall degrading enzymes. Grass and Forage Science 48: 70-78. Weiss, W. P., D. G. Chamberlain, and C. W. Hunt. 2003. Feeding silages. Pages 469–504 in Silage Science and Technology. Agronomy Monograph No. 42. ASA-CSSA-SSA, Madison, WI. Westlake, K., R. I. Mackie, and M. F. Dutton. 1989. In vitro metabolism of mycotoxins by bacterial, protozoal and ovine ruminal fluid preparations. Animal Feed Science and Technology 25: 169-178. Whiter, A., and L. Kung Jr. 2001. The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of alfalfa silage. Journal of Dairy Science 84: 2195-2202. Whitlow, L. W., and W. M. Hagler. 2005. Mycotoxins in dairy cattle: occurrence, toxicity, prevention and treatment. Proceeding of the Southwest Nutrition Conference 124-138. Wieringa, G. 1960. Some factors affecting silage fermentation. Proceedings 8th Int. Grassld Congr. 1960. Wilkinson, D. K., G. J. Pirelli, and H. H. Meyer. 1987. Conserving forage as round bale silage. Oregon State University Extention Circular 1259. Winters, A. L., R. Fychan, and R. Jones. 2001. Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance. Grass and Forage Science 56: 181-192. Woolford, M. 1990. The detrimental effects of air on silage. Journal of Applied Microbiology 68: 101-116. Woolford, M. K. 1975. Microbiological screening of the straight chain fatty acids (c1‐c12) as potential silage additives. Journal of the Science of Food and Agriculture 26: 219-228. Woolford, M. K. 1984. The silage fermentation. M. Dekker, New York. Yazar, S., and G. Z. Omurtag. 2008. Fumonisins, trichothecenes and zearalenone in cereals. International Journal of Molecular Sciences 9: 2062-2090. Yiannikouris, A., and J. P. Jouany. 2002. Mycotoxins in feeds and their fate in animals: A review. Animal Research 51: 81-99. Yitbarek, M. B., and B. Tamir. 2014. Silage Additives: review. Open Journal of Applied Sciences 04: 258-274. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70763 | - |
dc.description.abstract | 本研究目的在探討以丙酸銨或乳酸菌作為添加劑對國產芻料半乾青貯草發酵與保存的效果。試驗採用真空包裝袋製作實驗室規模的半乾青貯草,試驗牧草為國產苜蓿、尼羅草以及盤固草,試驗分成兩個部分,第一部分在探討不同添加量的丙酸銨(鮮重的0%、1.5%、3.0%)以及兩種發酵狀態(真空完好沒有空氣滲入的V組和以26G針頭製造空氣滲入的A組),採3×2複因子設計,共六組處理組。第二部分在探討不同乳酸菌組合對於國產半乾青貯草的影響,使用的乳酸菌包含Lactobacillus buchneri(L. buchneri, LB)、Lactobacillus plantarum(L. plantarum, LP)以及Pediococcus acidilactaci(P. acidilactaci, PA),試驗組別共四組包含對照組、3.0%丙酸銨處理組、兩種乳酸菌組合處理組(LB+ LP)和三種乳酸菌組合處理組(LB+ LP +PA),每株乳酸菌添加劑量皆為每克鮮草1×106菌落數,所有半乾青貯草製作均為有空氣滲入的發酵狀態。
第一部分丙酸銨添加試驗中,在真空完好沒有空氣滲入的組別,在採樣過程中苜蓿半乾青貯草未出現目測發霉外觀、尼羅草和盤固草半乾青貯皆出現1包目測發霉,然而,在有空氣滲入下對照組出現較高比例的目測發霉,在苜蓿對照組有將近4成的目測發霉、尼羅草對照組有高達6成的目測發霉、盤固草對照組有高達8成的目測發霉,在兩次的試驗結果中,不論丙酸銨或乳酸菌添加都使目測發霉數量降至1到2成;丙酸銨的添加會降低半乾青貯草發酵過程中的乳酸產量,其中又以3.0%丙酸銨添有較低的乳酸產量(p < 0.05);在苜蓿半乾青貯草試驗中,乳酸菌的添加在發酵初期有較高的乳酸產量(p < 0.05),在發酵後期則出現較高的乙酸產量(p < 0.05),不過pH值未能降至青貯穩定的臨界pH值4.75 (適用於乾物率40%的作物中),黴菌毒素ZEA和DON有超標的現象;在尼羅草試驗中,乳酸菌的添加組在發酵初期有較高的乳酸產量(p < 0.05)以及較低的pH值(p < 0.01),發酵後期乳酸產量下降顯著低於對照組(p < 0.01)、乙酸產量上升但沒有顯著高於對照組,pH值在發酵後期對照組為4.5、乳酸菌組為4.3,皆低於pH臨界值4.75,且發酵過程未檢測出超標的黴菌毒素濃度;在盤固草試驗中,第二年作物水分較高(大於70%),試驗結果在發酵產物上不理想,發酵初期乳酸菌添加組並沒有出現較高的乳酸產量,發酵後期不論對照組或乳酸菌處理組都出現高濃度的丁酸濃度(2-3 % DM basis),顯示在較高水分盤固草作物乳酸菌的添加仍發酵不易,不過發酵過程未檢測出超標的黴菌毒素濃度。 綜合上述,在丙酸銨和乳酸菌的添加組對於在有空氣滲入情形下,能有效抑制發霉現象,在黴菌毒素檢測中只有苜蓿出現較高的DON和ZEA濃度,其餘尼羅草和盤固草雖然發霉現象嚴重但未出現毒素超標的現象,在有機酸的產物以乳酸菌添加組有較好的產酸效果。 | zh_TW |
dc.description.abstract | The purpose of this study was to investigate the effect of ammonium propionate or lactic acid bacteria as an additive on the fermentation and preservation of haylage of domestic forages. The test haylage was made in a laboratory scale using vacuum bags. The experimental forage consisted of domestic Alfalfa, Nilegrass and Pangolagrass. The study was divided into two parts. The first part was to investigate the different levels of ammonium propionate (0, 1.5, 3.0 % of fresh weight) addition under two fermentation conditions (V group for vacuum condition without air infiltration and A group for air infiltration condition). The experimental design is 3×2 factorial design with a total of six treatment groups. The second part was to evaluate the effects of different lactic acid bacteria combination on haylage making. The lactic acid bacteria used include Lactobacillus buchneri (L. buchneri, LB), Lactobacillus plantarum (L. plantarum, LP) and Pediococcus acidilactaci (P. acidilactaci, PA). There were four groups including the control group, 3.0 % ammonium propionate, the mixture of two kinds of lactic acid bacteria (LB + LP) group and the mixture of three kinds of lactic acid bacteria (LB + LP + PA) group. The dosage of innoculant was 1 × 106 of fresh grass per gram for each lactic acid bacterium. All haylages were made under air infiltration condition.
In the first part of the ammonium propionate addition test, the Alfalfa haylage did not show visually mouldy appearance during the sampling process under vacuum condition without air infiltration; in addition, the Nilegrass and Pangolagrass haylage only got 1 mouldy appearance. However, there is higher proportion of visually mouldy appearance occurred in the control group under air infiltration condition, and there is nearly 40% of mouldy appearance in Alfalfa haylage, up to 60% mouldy appearance in Nilegrass, up to 80% mouldy appearance in Pangolagrass. No matter ammonium propionate or lactic acid bacteria was added, the moldy appearance was reduced to 10 to 20%. In the fermentation product, the addition of ammonium propionate reduced the production of lactic acid. Especially in 3.0% ammonium propionate treatment, lactic acid production was low in all forages (p < 0.05). In the Alfalfa haylage, treatment of lactic acid bacteria produced more lactic acid in the initial fermentation stage (p < 0.05), and higher acetic acid production in the later fermentation stage (p < 0.05), but the pH value did not drop to the critical pH 4.75 (suitable for crops with a dry matter rate of 40%), the mycotoxins ZEA and DON were excessive. In the Nilegrass haylage, treatment of lactic acid bacteria had better acid production and higher lactic acid yield in the early fermentation (p < 0.05), as well as lower pH (p < 0.01). In late fermentation, lactic acid was significantly lower than control group (p < 0.01). Acetate production increased but not significant different than control group. The pH value was 4.5 in the control group, 4.3 in lactic acid bacteria group, both of them were lower than the critical pH value of 4.75, and the fermentation did not show excessive mycotoxin concentration. In the Pangolagrass haylage, the moisture was higher (greater than 70%) in second part test, showing no satisfactory results in fermentation products. The lactic acid bacteria-added group did not show higher lactic acid production in the early stage of fermentation. Both the control group and lactic acid bacteria treatment groups showed high concentration of butyric acid (2-3% DM basis), indicating that the addition of lactic acid bacteria in Pangolagrass with higher water content was still difficult to ensure better fermentation, but no excessive mycotoxin concentrations were detected. In conclusion, the addition of ammonium propionate and lactic acid bacteria can effectively inhibit mouldy appearance under air infiltration condition. Only high levels of DON and ZEA were observed in Alfalfa haylage. In Nilegrass and Panolagrass haylage, there was no high mycotoxin concentration even with mouldy appearance. In fermentation products, lactic acid bacteria-added group had better acid production. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:37:36Z (GMT). No. of bitstreams: 1 ntu-107-R05626015-1.pdf: 8290954 bytes, checksum: 6d7c26e84b3144189c8da0dcb4ecd710 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 謝誌 I
摘要 II ABSTRACT IV 目錄 VII 圖次 X 表次 XI 緒言 1 壹、 文獻探討 2 一、 國產芻料現況 2 (一) 苜蓿 (Alfalfa) 2 (二) 尼羅草(Nilegrass) 3 (三) 盤固草(Pangolagrass) 3 二、 芻料的保存方法 4 (一) 乾草調製(hay making) 4 (二) 青貯草調製(silage making) 4 (三) 半乾青貯草調製(haylage or bale silage making) 5 三、 青貯發酵(ensiling) 7 (一) 有氧階段(aerobic phase) 8 (二) 發酵階段(fermentation phase) 8 (三) 發酵穩定階段(stable phase) 10 (四) 開封餵飼階段(feedout phase) 11 四、 青貯添加劑(silage additives) 12 (一) 青貯添加劑種類 12 (二) 發酵促進劑(fermentation stimulants) 14 (三) 發酵抑制劑(fermentation inhibitors) 17 五、 青貯發酵後產物評估 19 (一) 乳酸(lactic acid) 20 (二) 揮發性脂肪酸(volatile fatty acids) 21 (三) 氨態氮(ammonia nitrogen) 22 六、 青貯中的黴菌毒素 23 (一) 常見的黴菌毒素 24 (二) 黴菌毒素危害標準 30 七、 實驗室規模(laboratory scale)青貯製作 33 貳、材料方法 34 一、 試驗牧草與添加劑 34 (一) 苜蓿(Alfalfa) 34 (二) 尼羅草(Nilegrass) 34 (三) 盤固草(Pangolagrass) 34 (四) 試驗的青貯添加劑 35 二、 試驗設計 36 (一) 採樣時間與分析項目 37 (二) 半乾青貯草製作流程 38 (三) 試驗噴灑溶液配置 39 (四) 微波爐水分估測 40 三、 採樣與分析項目 41 (一) 採樣位置與半乾青貯均質 41 (二) 黴菌與酵母測定 41 (三) 揮發性脂肪酸與乳酸分析 42 (四) 氨態氮分析 43 (五) 水溶性碳水化合物(WSC)分析 44 (六) 黴菌毒素萃取與分析 45 (七) 開封後穩定性試驗 46 四、 統計分析 47 參、結果 48 一、 第一年丙酸銨添加劑試驗結果 48 (一) 苜蓿半乾青貯草 48 (二) 尼羅草半乾青貯 55 (三) 盤固草半乾青貯 61 二、 第二年乳酸菌與丙酸銨添加試驗 67 (一) 苜蓿半乾青貯草 68 (二) 尼羅草半乾青貯 78 (三) 盤固草半乾青貯 89 肆、討論 99 一、 綜合試驗討論 99 (一) 發霉現象 99 (二) 黴菌對於半乾青貯草保存的危害 100 (三) 黴菌毒素的檢測 102 (四) 發酵後有機酸產物 102 二、 丙酸銨在現場半乾青貯草製作的應用性 106 三、 乳酸菌在現場半乾青貯草製作的應用性 106 伍、結論 108 陸、參考資料 109 | |
dc.language.iso | zh-TW | |
dc.title | 評估添加丙酸銨或接種乳酸菌對國產芻料半乾青貯草製備之成效 | zh_TW |
dc.title | Evaluating the Effect of Adding Ammonium Propionate or Lactic Acid Bacterium Inoculant on Domestic Haylage Making | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王翰聰,李春芳,王紓愍 | |
dc.subject.keyword | 苜蓿半乾青貯草,尼羅草半乾青貯,盤固草半乾青貯,丙酸銨,乳酸菌,黴菌,黴菌毒素, | zh_TW |
dc.subject.keyword | Alfalfa haylage,Nilegrass haylage,Pangolagrass haylage,ammonium propionate,lactic acid bacteria,mould,mycotoxin, | en |
dc.relation.page | 118 | |
dc.identifier.doi | 10.6342/NTU201802596 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-08 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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