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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張繼堯(Chi-Yao Chang) | |
dc.contributor.author | Pei-Ru Wang | en |
dc.contributor.author | 王珮儒 | zh_TW |
dc.date.accessioned | 2021-06-17T04:37:31Z | - |
dc.date.available | 2020-08-09 | |
dc.date.copyright | 2018-08-09 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-08 | |
dc.identifier.citation | 許博婷。2017。神經壞死病毒外套蛋白與組蛋白H3.3在石斑魚腦細胞株中之交互作用。國立臺灣大學漁業科學研究所碩士論文。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70761 | - |
dc.description.abstract | 神經壞死病毒( Nervous necrosis virus, NNV)是感染超過 50 種重要養殖海水魚類幼魚嚴重災情的致病病毒,此病毒近幾十年來危害全世界魚苗養殖場,造成養殖業重大損失。為了瞭解 NNV 與宿主細胞間的交互作用,本實驗室利用 NNV 類病毒顆粒( Virus-like particle, VLP)固定在樹脂上,作為親和性管柱,並把石斑魚腦細胞(GB cell)蛋白質萃取液加入管柱,沖提出的產物以銀染( Silver staining)及病毒鋪覆蛋白試驗( Virus overlay protein binding assay, VOPBA)及 LC-MS/MS 進行分析,推測在 30 kDa 上的條帶為核醣體蛋白 L7a。為了進一步了解 NNV 外套蛋白與核醣體蛋白 L7a 間的交互作用,先用大腸桿菌表現出重組 L7a,再做 VOPBA 確認L7a 與 NNV 外套蛋白有交互作用,且兩者結合的區域在核醣體蛋白 RPL7a 的 1-85 a.a.。用免疫螢光染色發現核醣體蛋白 L7a 與 NNV 外套蛋白在轉殖後 15 小時,重組表現的 NNV 外套蛋白與核醣體蛋白 L7a 共同分布在核仁;而轉殖重組核醣體蛋白 RPL7a 後再以 NNV 感染則發現原本聚集在核仁的核醣體蛋白 RPL7a 會離開核
仁,並散布在整個細胞質。另外病毒感染 GB 細胞後收集不同時間點的細胞總蛋白,發現核醣體蛋白 RPL7a 在感染後並無明顯變化,但以 RNAi 降低核醣體 RPL7a轉錄量卻可促進神經壞死病毒複製。此研究證明 GB 細胞的核醣體蛋白 RPL7a 會與神經壞死病毒外套蛋白結合,在病毒感染 GB 細胞後改變核醣體蛋白 RPL7a 在GB 細胞的分布區域,而降低核醣體 L7a 可能會促進神經壞死病毒增殖。 | zh_TW |
dc.description.abstract | Nervous necrosis virus ( NNV) is a positive sense single-stranded RNA virus, it infects more than 50 species of marine fish and causes significant economic losses. To investigate the interaction of NNV coat protein and grouper brain ( GB) cell, we set up the NNV virus-like particle-coupled affinity column. By LC-MS/MS analysis, we define the 30 kDa band in SDS-polyacrylamide gel is ribosomal protein L7a (RPL7a). The interaction between NNV coat protein and RPL7a were further confirmed by virus-overlay protein binding assay ( VOPBA), and the binding region of RPL7a against NNV coat protein was defined to 1-85 a.a. By cotransfection of expression constructs which encoded NNV coat protein and RPL7a with HA or Myc tags, we find that both NNV coat protein and RPL7a colocalized in nucleolus 15 hr after cotransfection . With NNV infection, recombinant RPL7a was found to translocate from nucleolus to cytoplasm. The protein level of RPL7a in GB cell was constant during NNV infection by Western analysis. However, the amount of RNA2 in NNV infected GB cells was increase slightly after RPL7a knockdown by RNAi. Taken together, these results showed that the interaction between NNV coat protein and RPL7a relocated RPL7a from nucleolus to cytoplasm and influenced the propogation of NNV. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:37:31Z (GMT). No. of bitstreams: 1 ntu-107-R05b45005-1.pdf: 2529788 bytes, checksum: 4b612b324ec843a954623d1e975563a0 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 中文摘要………………………………………………………………………….….Ⅰ
英文摘要………………………………………………………………………….….II 目錄…………………………………………………………………………….…...III 圖目次……………………………………………………………………….……….Ⅴ 表目次……………………………………………………………………....………. VI 第一章、前言 1.1神經壞死病毒…...……………………………………………………………...1 1.2神經壞死病毒與宿主細胞蛋白之交互作用…………………………………..2 1.3 核醣體蛋白 RPL7.……………………………………………………...….......3 1.4 病毒與核醣體的交互作用.………………………….…………………………5 1.5 研究動機...…………………………………………………………………...…6 第二章、材料與方法 2.1 GB3 細胞及 NNV 病毒及培養…....……………………………………..……..7 2.2 GB3 細胞結合蛋白之分離與鑑定…………...…...………………………….....8 2.3 基因選殖………………………………....…………..………………...………11 2.4 純化大量質體…..…………...…………………………………………..…….18 2.5 表現重組蛋白質………………………………………………………………19 2.6 純化重組蛋白…………………………………………………………………21 2.7 細胞免疫染色…………………………………………………………………22 2.8 神經壞死病毒感染對核醣體蛋白 RPL7a 的蛋白表現影響………………...24 2.9 抑制核醣體蛋白 RPL7a 在細胞之表現量.…………………………………..26 第三章、結果 3.1 類病毒顆粒親和性管柱分離蛋白..………………………………...…..…….32 3.2 核醣體蛋白 RPL7a 的序列特性…………………………………….……….32 3.3 表現點帶石斑魚腦細胞之核醣體蛋白 RPL7a 的重組蛋白…………….….33 3.4 重組核醣體蛋白 RPL7a 的 VOPBA………………………………………...34 3.5 核醣體蛋白 RPL7a 與神經壞死病毒外殼蛋白在 GB 細胞之相對位置…..34 3.6 神經壞死病毒感染後核醣體蛋白 RPL7a 之蛋白表現變化………………..35 3.7 抑制核醣體 RPL7a 的 mRNA 表現對神經壞死病毒感染之影響…………35 第四章、討論……………………………………………………..…………..……….36 第五章、參考文獻………………………………………………..………..………….39 圖目次 Figure 1: Virus overlay protein binding assay ( VOPBA) of glycine‧HCl eluted NNV VLP binding proteins from VLP affinity column ………….……………………46 Figure 2: Comparision of amino acid sequence of orange-spotted grouper ribosomal protein RPL7a with that of other model-organisms……………………………..47 Figure 3: Comparision of nucleotide sequence of GK cell with that of GB cell………………………………………………………………………………..48 Figure 4: Comparision of amino acid sequence of ribosomal protein RPL7a between GK and GB cells………………………………………………………………....49 Figure 5: Expresion of recombinant ribosomal protein L7a…………………………...50 Figure 6: Expression of recombinant RPL7a deletions………………………………...51 Figure 7: Purification of recombinant ribosomal protein L7a and its different deletion…………………………………………………………………………...52 Fig 8: VOPBA of rRPL7a and its different deletions…………..………………………53 Figure 9: Cotransfection of pcDNA-RPL7a-HA and pcDNA-NNVCP-Myc………….54 Figure 10: Colocalization of recombinant RPL7a and NNV particle…………………..55 Figure 11: Protein level of ribosomal protein L7a during NNV infection in GB cells……………………………………………………….……………………...56 Figure 12: RT-PCR detection of NNVCP in RPL7a RNAi treated GB cells after NNV infection…………………………………………….…………………………….57 表目次 Table 1: Primers used in this study……………………………………………………..58 Table 2: Results of LC-MS/MS………………………………………………………...59 | |
dc.language.iso | zh-TW | |
dc.title | 神經壞死病毒外套蛋白與核醣體L7a在石斑魚腦細胞之交互作用 | zh_TW |
dc.title | Interaction of nervous necrosis virus coat protein with ribosomal protein L7a in grouper brain cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖文亮(Wen-Liang Liao),吳惠南(Huey-Nan Wu),鄭朝安(Chao-An Cheng) | |
dc.subject.keyword | 神經壞死病毒,外套蛋白,核醣體蛋白 RPL7a,病毒鋪覆蛋白試驗,類病毒顆粒,親和性管柱, | zh_TW |
dc.subject.keyword | Nervous necrosis virus ( NNV),coat protein,ribosomal protein RPL7a,VOPBA,virus-like particle,affinity column, | en |
dc.relation.page | 59 | |
dc.identifier.doi | 10.6342/NTU201802281 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-08 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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