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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70584完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林思洸(Sze-Kwan Lin) | |
| dc.contributor.author | HUI CHIEN | en |
| dc.contributor.author | 錢蕙 | zh_TW |
| dc.date.accessioned | 2021-06-17T04:31:45Z | - |
| dc.date.available | 2021-08-30 | |
| dc.date.copyright | 2018-08-30 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-10 | |
| dc.identifier.citation | Reference
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70584 | - |
| dc.description.abstract | 類風溼性關節炎是一種由多種細胞(包含單核細胞, 關節滑膜細胞,內皮細胞, 其他免疫細胞)共同調控的系統性疾病。類風溼性關節炎也是心血管疾病的危險因子。在病患的關節滑液中發現與atheroma有類似的細胞組成。27-hydroxycholesterol會加劇粥狀動脈硬化。而在關節炎之中，類風濕性關節炎滑液細胞(RASF)扮演著重要的腳色。本實驗希望探討HepG2(人類肝癌細胞株)在fatty acid的刺激下 sterol 27-hydroxylase (CYP27A1) 的調控，以及RASF是否會受到27-hydroxycholesterol的各種影響來探討肥胖及膽固醇代謝對於類風濕性關節炎的影響。實驗結果發現，人類肝癌細胞株在受到oleic acid(油酸)的刺激下，CYP27A1的蛋白表現上升。並且在培養液中的27HC含量上升。另外的實驗中，27HC會促進RASF細胞的增生；並且造成多種發炎介質的濃度上升。且在動物實驗中發現肥胖的飲食確實能加劇關節炎的發展，發炎細胞的浸潤與滑液細胞的增生，而抑制CYP27A1的藥物anastrozole使用的組別小鼠在脂肪肝與關節炎的症狀都有減輕。總結上述的發現，肥胖會使肝臟細胞脂質代謝改變而CYP27A1增加，並產生較多量的27HC，而27HC可能是造成關節炎加劇的原因。 | zh_TW |
| dc.description.abstract | Rheumatoid arthritis is a systemic disease that is regulated by a variety of cells, including monocytes, synovial cells, endothelial cells, and other immune cells. Rheumatoid arthritis is also a risk factor for cardiovascular disease. A similar cellular composition to atheroma was found in the synovial fluid of the patient. 27-hydroxycholesterol exacerbates atherosclerosis. In arthritis, rheumatoid arthritis synovial cells (RASF) play an important role. This experiment hopes to investigate the regulation of sterol 27-hydroxylase (CYP27A1) stimulated by fatty acid in HepG2 (human hepatocarcinoma cell line), and whether RASF will be affected by 27-hydroxycholesterol to explore obesity and cholesterol metabolism for rheumatoid arthritis. Impact. The experimental results showed that the protein expression of CYP27A1 was increased in human hepatoma cell lines stimulated by oleic acid (oleic acid). And the 27HC content in the culture solution increased. In another experiment, 27HC promoted the proliferation of RASF cells; and caused an increase in the concentration of various inflammatory mediators. And in animal experiments, it is found that the diet of obesity can indeed aggravate the development of arthritis, the infiltration of inflammatory cells and the proliferation of synovial cells, while the mice used in the anastrozole that inhibit CYP27A1 have symptoms of fatty liver and arthritis. Reduced. Summarizing the above findings, obesity will change the lipid metabolism of liver cells and increase CYP27A1, and produce a larger amount of 27HC, which may be the cause of arthritis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T04:31:45Z (GMT). No. of bitstreams: 1 ntu-107-R04422003-1.pdf: 1251133 bytes, checksum: d78cd7857881e071cfd423ea929e6ef4 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 目錄
口試委員審定書…………………………………………………………………………i 謝誌 II 中文摘要 III 英文摘要 iV 第一章導論 3 1.1 類風溼性關節炎 3 1.2類風溼性關節炎的細胞變化 3 1.2.1 RASF的活化 3 1.2.2 發炎細胞的活化 4 1.3類風溼性關節炎與肥胖的關係 4 1.3.1肥胖與關節炎的關係 4 1.3.2 肥胖與關節炎的致病機制 4 1.4膽固醇在人體內的恆定 5 1.4.1膽固醇 5 1.4.2 LXRs 5 1.5 27HC 5 1.5.1 27-HC的組成…………………………………………………………………………………….5 1.6 Oxysterol 扮演的腳色 6 1.6.1 oxysterol 6 1.6.2 Sterol 27-hdroxylase 6 第二章 實驗目的 7 第三章材料與方法 10 3.1 實驗細胞株 10 3.2 細胞繁殖速率分析 10 3.2.1.2.1 細胞計數 10 3.2.2 RASF細胞對27HC之增生率分析 10 3.3蛋白質的萃取 10 3.4西方點墨法（Western blot） 11 3.5 OA(oleic acid)對HepG2細胞在Cyp27A1與27HC表現的影響………………….12 3.5.1不同濃度的OA對HepG2細胞在Cyp27A1表現的影響………….…….12 3.5.2 OA刺激對HepG2細胞在27HC表現的影響 13 3.6 27HC對RASF的CCL2與IL-6的影響 14 3.6.1 27HC對RASF的CCL2的影響 14 3.6.1 27HC對RASF的IL-6的影響……………………………………………………………14 3.7 Anastrozole對於OA刺激CYP27A1蛋白表現量的影響 15 3.8 動物實驗 16 第四章實驗結果 17 4.1 OA濃度對HepG2 細胞的影響 17 4.1.1 OA濃度影響 CYP27A1在HepG2的蛋白表現 17 4.1.2 ox-LDL CYP27A1在HepG2的蛋白表現影響不顯著 17 4.1.3 OA刺激對於HepG2培養液中27HC含量的影響 17 4.1.4 OA4.1.4 anastrozole對於OA刺激HepG2後培養液中27HC含量的影響………………………………………………………………………………………………………….……17 4.1.5 anastrozole對於OA刺激HepG2後培養液中27HC含量的影響……17 4.2 27-hydroxycholesterol對RASF 細胞增生率的影響 18 4.2.1 27-hydroxycholesterol對RASF細胞增生率的影響 18 4.2.2不同27-hydroxycholesterol刺激時間對RASF 細胞增生的影響 18 4.3 27-hydroxycholesterol對RASF 釋出發炎介質的影響 19 4.3.1 27-hydroxycholesterol刺激對於RASF培養液中CCL2含量的影響 19 4.3.2不同的27-hydroxycholesterol刺激時間對於RASF培養液中CCL2含量的影響 19 4.3.3不同的27-hydroxycholesterol刺激時間與濃度對於RASF培養液中IL-6含量的影響 19 4.4 免疫細胞對於27-hydroxycholesterol的反應…………………………………………….19 4.5 動物實驗 19 4.5.1不同的不同飲食條件下的小鼠的關節炎發展差異………………………...19 4.5.2 不同的不同飲食條件下的小鼠的脂肪肝發展差異 19 第五章討論 20 5.1 OA對HepG2細胞的影響 20 5.2 27-hydroxycholesterol對RASF細胞的影響 20 5.3 27-hydroxycholesterol的產生與效益的連結 21 參考文獻 22 附錄 28 | |
| dc.language.iso | zh-TW | |
| dc.subject | 27羥基膽固醇 | zh_TW |
| dc.subject | 類風溼性關節炎 | zh_TW |
| dc.subject | rheumatoid arthritis | en |
| dc.subject | 27-hydroxycholesterol | en |
| dc.title | 27羥基膽固醇對類風濕性關節炎的影響 | zh_TW |
| dc.title | The impact of 27-hydroxycholesterol on rheumatoid arthritis propagation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭生興,洪志遠 | |
| dc.subject.keyword | 類風溼性關節炎,27羥基膽固醇, | zh_TW |
| dc.subject.keyword | rheumatoid arthritis,27-hydroxycholesterol, | en |
| dc.relation.page | 41 | |
| dc.identifier.doi | 10.6342/NTU201802666 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-13 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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