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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李芳仁(Fang-Jen S. Lee) | |
dc.contributor.author | Yan-Ting Chen | en |
dc.contributor.author | 陳彥廷 | zh_TW |
dc.date.accessioned | 2021-06-17T04:29:10Z | - |
dc.date.available | 2023-10-03 | |
dc.date.copyright | 2018-10-03 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-13 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70482 | - |
dc.description.abstract | 腺嘌呤核苷二磷酸核糖化相似因子 (Arf-like protein, Arl) 在細胞體內扮演相當重要的角色,尤其是其調控囊泡運輸的功能。在釀酒酵母中,第一腺嘌呤核苷二磷酸核糖化相似因子 (Arl1) 負責在反式高爾基氏體網路 (trans-Golgi network) 上招募高爾基體蛋白Imh1,但是其詳細的功能並不是十分清楚。在我們先前的研究中,我們發現當細胞遭遇內質網壓力時,會促進Arl1以及其下游Imh1的活化。總而言之,我們仍不了解其作用的機轉以及活化的目的。在本篇研究中,我們發現當細胞遭遇內質網壓力時,被活化的Arl1以及Imh1會專一性的調控細胞中的反向運輸。有趣的是,Imh1的氨基端對於其功能是相當重要的,氨基端的突變會導致內質網壓力下反向運輸的缺陷。我們的研究指出,Imh1會透過招募一個被提出可能參與囊泡融合的蛋白質Sft2來達到其功能,雖然我們還需要繼續探討Sft2被Imh1所調控的機轉。與Imh1相同的是,Sft2的氨基端同樣也對於內質網壓力下的反向運輸非常重要。氨基端的突變也同樣會造成反向運輸無法運行。總結本篇,我們的研究意謂著細胞囊泡運輸系統在內質網壓力下的重要性。更多的是,我們提出了一個有趣的理論模式說明細胞中Imh1的功能並闡述了它的重要生理意義。 | zh_TW |
dc.description.abstract | Arf-like proteins (Arls) are important regulators involved in a diversity of biological events, especially vesicle trafficking. In Saccharomyces cerevisiae, Arl1 acts to recruit a golgin protein Imh1 to the trans-Golgi network (TGN). However, besides the role of Imh1 as a high-copy suppressor of Ypt6, an important Rab protein mediating endosome-to-Golgi trafficking, less is known about it physiological significance. In previous studies (Hsu et al., 2016), they demonstrated that the Unfolded-Protein Response (UPR) augmented the activity of Arl1 and the subsequent Imh1 recruitment, but it remained to be elucidated on its specific functions under ER stress. Here, we showed that the UPR-activated Arl1 and Imh1 act to specifically maintain the endosome-to-Golgi trafficking when cell encounters ER stress. Moreover, this regulation does not simply depend on GARP complex recruitment, an important tethering complex, to affect retrograde trafficking. We found that the first five amino acids at Imh1 N-terminus is required for its function, as it contributes to the recruitment of Sft2, a tetra-spanning membrane protein involved in vesicle fusion. The N-terminus of Sft2 is responsible for facilitating the transport of Snc1 and Tlg1 and promoting retrograde trafficking. Together, our study is one of the first to demonstrate the physiological function of golgin Imh1 and elucidate the importance of the trafficking machinery in response to ER stress. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:29:10Z (GMT). No. of bitstreams: 1 ntu-107-R05448006-1.pdf: 6321498 bytes, checksum: 4afa5e72deebe6ced64cdb6de194d123 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | Tabel of Contents
口試委員審定書 1 致謝 2 中文摘要 6 Abstract 7 Introduction 8 ADP-ribosylation Factors 9 ADP-ribosylation Factor-like proteins (Arls) 10 Tethering Factors and Vesicle Targeting 13 Unfolded Protein Response (UPR) 20 Material and Methods 25 Tables 36 Table 1. Yeast strains used in this study 36 Table 2. Plasmids used in this study 38 Table 3. Antibodies used in this study 40 Results 41 Part I. The Function of Golgin Imh1 upon Unfolded-protein response in Saccharomyces cerevisiae. 41 ER-stress induces the upregulation of Arl1 activity and subsequent Imh1 recruitment that acts to facilitate retrograde trafficking. 41 The Ire1-Syt1-Arl1-Imh1 cascade are required for maintaining proper retrograde trafficking upon ER stress 44 The extreme N-terminal of Imh1 is mandatory for regulating retrograde trafficking upon UPR. 45 Imh1 mediates endosome-to-Golgi trafficking through a mechanism different from Ypt6 upon UPR 47 Sft2 might be involved in Imh1-mediated regulation of endosome-to-Golgi trafficking upon ER stress. 49 Part II The negative-feedback regulation between Arl1 and Arl3. 52 Constitutive active Arl1Q72L interact with constitutive active Arl3Q78L both in vitro and in vivo. 52 Active Arl1 displayed an enhanced-accumulated distribution upon the expression of highly active Arl3. 53 The expression of active Arl1 and Arl3 functions to exert a negative-feedback regulation and displayed a blockage of downstream Gas1 transport. 55 Disrupting the interaction between Arl1Q72L and Arl3Q78L disturbs the negative-feedback of Arl1. 57 Discussion 59 Part I. The Function of Golgin Imh1 upon Unfolded Protein Response in Saccharomyces cerevisiae. 59 The Physiological importance of Imh1 upon ER stress 60 The role of Sft2 in mediating endosome-to-Golgi trafficking upon ER stress. 61 The physiological outcome of Imh1-mediated retrograde trafficking. 62 The differential role of Imh1 in suppression of ypt6∆ cells and upon ER stress. 64 Part II The negative-feedback regulation between Arl1 and Arl3. 65 Figures 68 Figure 1. ER stress induces elevated-activation of Arl1 and subsequent Imh1 recruitment at TGN. 68 Figure 2. Arl1 and Imh1 are involved in the maintenance of proper retrograde trafficking upon ER stress. 69 Figure 3. Arl1 and Imh1 interdependently facilitates retrograde transport upon ER stress. 70 Figure 4. Arl1 and Imh1 specifically regulate the transport of Snc1 and Tlg1 but not the overall integrity of different compartments upon ER stress. 71 Figure 5. The unfolded protein response-mediated Syt1 phosphorylation is required for proper retrograde transport upon ER stress. 72 Figure 6. Phosphor-mimetic mutant of Syt1 is non-sufficient to restore Snc1 and Tlg1 transport upon ER stress in the absence of Ire1. 73 Figure 7. The extreme N-terminal of Imh1 is crucial for its function in regulating retrograde trafficking. 75 Figure 8. The Imh1 N-terminal mutants are still capable of protecting Arl1 from the hydrolysis of Gcs1 and maintain its localization. 76 Figure 9. GARP complex colocalizes with Arl1 and Imh1 both in normal conditions and the presence of ER stress. 77 Figure 10. Imh1 is not involved in regulating the recruitment of GARP complex and affecting the TGN localization of Ypt6 when ER stress is present. 78 Figure 11. Ypt6 is incapable of suppressing defects in Imh1-mediated retrograde trafficking under ER stress conditions. 79 Figure 12. Sft2 shows colocalization with Arl1 and Imh1 both in normal conditions and when UPR is activated. 80 Figure 13. Arl1 and Imh1 are involved in the recruitment of Sft2 to TGN upon ER stress. 81 Figure 14. Sft2 is required for proper retrograde trafficking without affecting the recruitment of GARP complex upon UPR. 83 Figure 15. The N-terminus of Sft2 is indispensable for its function in regulating retrograde trafficking upon ER stress. 84 Figure 16. Arl1, Imh1 and Snc1 contribute only partially to Tunicamycin resistance. 85 Figure 17. Working model of UPR-activated Arl1-Imh1-Sft2 cascade in regulating retrograde trafficking upon ER stress. 86 Figure 18. Arl1Q72L interacts with Arl3Q78L both in vivo and in vitro. 87 Figure 19. Expression of constitutive active Arl3 leads to the accumulation of Arl1 in the absence of GCS1. 88 Figure 20. The Arl3-Arl1 cascade are involved in the transport of the GPI-anchor protein Gas1. 89 Figure 21. Overexpression of Arl1Q72L exerts dominant negative effect on specific cargo transport that can be relieved by disruption of ARL3. 90 Figure 22. Coexpression of constitutive active Arl1 and Arl3 leads to severe Congo Red hypersensitivity. 91 Figure 23. Expression of Arl3Q78L leads to defective transport of Gas1 under constitutive active Arl1 in the absence of GCS1. 92 Figure 24. M2 mutant at the N-terminal of Arl1 diminishes the direct interaction between Arl1Q72L and Arl3Q78L in vitro. 94 Figure 25. The addition of M2 mutant upon Arl1Q72L relieved Congo Red hypersensitivity in coexpression of Arl3Q78L and Arl1Q72L. 95 Figure 26. Working Model of the hypothesized negative-feedback regulation between Arl1 and its upstream regulator Arl3. 96 Reference 97 | |
dc.language.iso | en | |
dc.title | 第一腺嘌呤核苷二磷酸核醣化因子相似蛋白和高爾基體蛋白Imh1於酵母菌之功能性探討 | zh_TW |
dc.title | Functional Characterization of small GTPase Arl1 and Golgin Protein Imh1 in Saccharomyces cerevisiae | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林敬哲(Jing-Jer Lin),鄧述諄(Shu-Chun Teng),陳瑞華(Ruey-Hwa Chen),王昭雯(Chao-Wen Wang) | |
dc.subject.keyword | 高爾基氏體,第一腺嘌呤核?二磷酸核糖化相似因子,高爾機體蛋白,內質網壓力,反向運輸, | zh_TW |
dc.subject.keyword | Arl1,Imh1,Sft2,UPR,ER stress,retrograde trafficking, | en |
dc.relation.page | 109 | |
dc.identifier.doi | 10.6342/NTU201802833 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-13 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
顯示於系所單位: | 分子醫學研究所 |
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