請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70412
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫岩章 | |
dc.contributor.author | Chen-Yu Tsai | en |
dc.contributor.author | 蔡鎮宇 | zh_TW |
dc.date.accessioned | 2021-06-17T04:27:37Z | - |
dc.date.available | 2019-08-19 | |
dc.date.copyright | 2018-08-19 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-13 | |
dc.identifier.citation | 江維翰。2013。十字花科根瘤病生物防治資源之開發與應用。國立台灣大學植物病理與微生物學系碩士論文。
安寶貞、李惠鈴、蔡志濃。1999。Phellinus noxius 引起果樹及觀賞植物褐根病之調查。植病會刊 8:61-66。 安寶貞、蔡志濃、張東柱、王姻婷。2005。台灣果樹及木本觀賞植物立枯型真菌性病害之調查與分布。植病會刊 14: 203-210 李韋辰。2016。放線菌及常用藥劑應用於樹木褐根病防治之研究。國立台灣大學植物病理與微生物學系碩士論文。 孫岩章。2015。談樹木褐根病之預防性及治療性注射防治策略。樹木疫病蟲害之醫療及健檢研討會論文集。台灣植物及樹木醫學學會。 孫岩章。2017。樹木褐根病成功預防及治療之藥劑注射及專業施作之必要性。2017植栽及樹木之健檢與醫療研討會論文集:35-54。 許如君、吳昌昱。2017。農用藥劑分類及作用機制檢索。第二版。行政院動植物防疫檢疫局。 曾德賜。2015。農藥藥理與應用。初版。國立中興大學出版中心。 傅春旭。2005。樹木褐根病之研究。國立台灣大學植物病理與微生物學系博士論文。 蔡志濃、安寶貞、謝文瑞。2005。抑制褐根病菌、白紋羽菌及南方靈芝菌之化學藥劑篩選。植物病理學會刊 14:115-124。 蔡志濃、謝文瑞、安寶貞。2008。氮肥與化學藥劑對果樹褐根病菌之影響與田間病害防治。植物病理學會刊 17:119-126。 蔡志濃。2008。褐根病菌之生物特性及分子診斷技術。國立中興大學植物病理系博士論文。 蔡志濃、安寶貞、謝文瑞。2010。褐根病之發生、診斷鑑定及防治。近年來我國作物重大病害之發生及其診斷、監測與防治研討會專刊:147-163。 蕭文偉、吳瑞鈺、孫岩章。2017。樹木褐根病菌子實體病原性及接種模式植物之研究。2017植栽及樹木之健檢與醫療研討會論文集:55-75。 Ann, P. J., Lee, H. L., and Huang, T. C. 1999. Brown root rot of 10 species of fruit trees caused by Phellinus noxius in Taiwan. Plant Disease 83:746-750. Ann, P. J., Chang, T. T.,and Ko, W. H. 2002 Phellinus noxius brown root rot of fruit and ornamental trees in Taiwan. Plant Disease 86:820-826. Blaedow, R. A., Juzwik, J., and Barber, B. 2010. Propiconazole distribution and effects on Ceratocystis fagacearum survival in roots of treated red oaks. Phytopathology 100:979-985. Chang, T. T. 1995a. Decline of nine tree species associated with brown root rot caused by Phellinus noxius in Taiwan. Plant Disease 79:962-965. Chang, T. T. 1995b. A selective medium for Phellinus noxius. European Journal of Forest Pathology 25:185-190. Chang, T. T. 1996. Suvival of Phellinus noxius in soil and in the roots of dead host plant. Phytopathology 86:272-276. Chang, T. T., Chang R. J. 1999. Generation of volatile ammonia from urea fungicidal to Phellinus noxius in infected wood in soil under controlled conditions. Plant Pathology 48:337-344. Chung, W. C., Huang, J. W., Huang, H. C., and Jen, J. F. 2003. Control, by Brassica seed pomace combined with Pseudomonas boreopolis, of damping-off of watermelon caused by Pythium sp. Canadian Journal of Plant Pathology 25:285-294. Corner, E. J. H. 1932. The identification of the brown rot fungus. Gardens' Bull, Straits Settlements 5:317-350. Cunningham, G. H. 1965. Polyporaceae of New Zealand. RE Owen, Government Printer. Fu, C. H., Hu, B. Y., Chang, T. T., Hsueh, K. L., and Hsu, W. T. 2012. Evaluation of dazomet as fumigant for the control of brown root rot disease. Pest Management Science 68:959-962. Garrett, S. D. 1970. Pathogenic root-infecting fungi. Cambridge University Press, Cambridge, NY. Gullino, M. L., Leroux, P., and Smith, C. M. 2000. Uses and challenges of novel compounds for plant disease control. Crop Protection 19:1-11. Intini, M. G. 1991. Some common species of tropical lignicolous fungi. Internation Journal of Tropical Plant Disease 9:1-14. Inoue, K., Tsurumi, T., Ishii, H., Park, P., and Ikeda, K. 2012. Cytological evaluation of the effect of azoxystrobin and alternative oxidase inhibitors in Botrytis cinerea. FEMS Microbiology Letters 326(1):83-90. Nandris, D., Nicole, M. and Geiger, J. P. 1987. Root rot disease of rubber trees. Plant Disease 71:298-306. Sahashi. N., Akiba, M., Ishihara, M. and Miyazaki, K. 2010. Cross inoculation tests with Phellinus noxius isolates from nine different host plants in the Ryukyu Islands, Southwestern Japan. Plant Disease 94:358-360. Sawada, K. 1928. Descriptive catalogus of Formosan fungi. IV 86-91. (in Japanese) Shigo, A. L., Marx, H. G. 1977. Compartmentalization of decay in trees. USDA Forest Service Agriculture Information Bulletin Number 405. 70 pp. Wang C., Liu W., Ding S., Wang Z., Zhang Y. 2010. Study on function of tebuconazole on Sclerotinia sclerotiorum. Chinese Journal of Oil Crop Sciences 32 (3):436–440. (in Chinese,with English summary) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70412 | - |
dc.description.abstract | 由 Phellinus noxius 引起之樹木褐根病為台灣最重要之樹木病害之一,除造成樹木萎凋死亡及經濟損失外,亦會威脅民眾生命及財產安全,是迫切需要防治及管理的樹木病害。褐根病菌殘存於木材組織作為感染源為樹木褐根病傳播的主要途徑之一,因此降低或除滅土壤及病組織中殘存之病原菌為褐根病管理中相當重要的課題。本研究旨在篩選防治褐根病可用的新藥劑,並探討藥劑注射、土壤藥劑澆灌或土壤改良劑處理對於褐根病菌殘存之影響。本研究將馬拉巴栗幼苗接種褐根病菌 PN401 菌株,用來模擬藥劑注射及殘存試驗。馬拉巴栗接種褐根病菌並發病後,埋在 BVB 栽培介質中1個月,仍可自組織再分離到褐根病菌。六種供試藥劑分別為亞派占、亞托環克座、貝芬四克利、白列克收欣、三泰隆及普克利。經稀釋平板法測試,有效成分濃度10 ppm 之亞派占、亞托環克座、貝芬四克利、三泰隆和普克利對褐根病菌菌絲生長抑制率可達100%,白列克收欣在1000 ppm 時抑制率僅77.4%。馬拉巴栗接種褐根病前,將六種受試藥劑稀釋1000倍進行預防注射,以亞派占、亞托環克座、白列克收欣及三泰隆較能有效抑制褐根病菌感染。而在治療性注射試驗中,任何藥劑經1000倍或100倍稀釋後注射,均無法有效防治褐根病。將馬拉巴栗接種褐根病後剖半作為模擬殘根,埋入 BVB 栽培介質並進行土壤藥劑澆灌、土壤改良劑及淹水處理,其中以淹水處理效果最佳,兩批次試驗分別於7天及3天後偵測不到褐根病菌殘存,菜籽粕處理效果次之,在第二批次試驗中於3天後檢測不到褐根病菌殘存。將罹病之田間榕樹殘根埋入 BVB 栽培介質,並進行亞派占、貝芬四克利、萄萄糖-麩胺酸之澆灌、菜籽粕混拌及淹水處理,其中仍以淹水處理之效果最佳,處理後1個月即無褐根病菌殘存,其他處理則無顯著之效果。田間褐根病病株經藥劑注射後,經採樣發現莖基部組織中褐根病菌之殘存率有下降之趨勢,但無法將褐根病菌完全除滅。罹病樹頭及病區土壤經藥劑澆灌處理,經採樣檢測褐根病菌殘存率亦有下降之趨勢,其中麵包樹樹頭基部在處理後近10個月已分離不到褐根病菌,但樹頭地下根部組織中褐根病菌之殘存情行仍屬未知,有待進一步研究探討。 | zh_TW |
dc.description.abstract | Brown root rot disease caused by Phellinus noxius is one of the most important tree diseases in Taiwan. In addition to causing decline of trees and economic loss, brown root rot disease also posts threat to people’s lives and property, which make it urgently needed for disease management. Long-term survival of P. noxius in infested wood tissue as primary inoculum is one of the main pathway for disease spreading. Therefore, it is critical for brown root rot disease management to reduce or eradicate the survival of P. noxius in infested tissue and soil. This study is aimed at selecting new fungicide available for disease control and evaluating the effects of trunk injection, soil drenching and soil amendment on survival of P. noxius. Seedlings of Malabar chestnut (Pachira aquatica) were inoculated with P. noxius (isolate PN401) for fungicides injection test and survival test in this study. P. noxius could be isolated from diseased tissue of Malabar chestnut which had buried in BVB substrates for 1 month. Fungicides including isopyrazam, azoxystrobin + cyproconazole, carbendazim + tetraconazole, boscalid + kresoxim-methyl, triadimenol and propiconazole are tested for their inhibition on P. noxius in this study. Mycelial growth of P. noxius was completely inhibited on the dilution PDA plate with 10 ppm isopyrazam, azoxystrobin + cyproconazole, carbendazim + tetraconazole, triadimenol or propiconazole, while the inhibition rate of mycelial growth only reached 77.4% on dilution plate with 1000 ppm boscalid + kresoxim-methyl. The 1000-fold dilution of each tested fungicide was preventively injected into the basal stem of malabar chestnut before inoculation with P. noxius. Isopyrazam, azoxystrobin + cyproconazole, boscalid + kresoxim-methyl or triadimenol treatment showed good control efficacy for P. noxius, while therapeutical injection after inoculation with the same fungicides showed no effect on disease control. Malabar chestnut stem infected with P. noxius was bisected, buried in BVB substrates and treated with fungicides drenching, soil amendment, or flooding. Flooding treatment showed the most effective on eradicating P. noxius which could not be isolated from infested tissue after 7 days or 3 days in two replicate tests. Rapeseed meal treatment also showed good effectiveness and eradicated P. noxius in 3 days in one replicate test. Root debris of Chinese banyan (Ficus microcarpa) infested with brown disease in fields was collected, buried in BVB substrates and treated with suspension of isopyrazam, carbendazim + tetraconazole, glucose-glutamate solution, rapeseed meal or flooding. The flooding treatment still showed the most effective on eradicating P. noxius which could not be isolated from root tissue after 1 month treatment, while other treatment showed no significant effects. Trees in fields were injected with fungicides for controlling brown root rot disease and tissue at basal stem was sampled. After injection, the survival rate of P. noxius in diseased tissue was reduced gradually but not eradicated. Diseased stumps and soil were drenched with fungicides and sampled. Results showed that the survival of P. noxius at stumps also reduced. At the stump of Artocarpus altilis, P. noxius could not even be detected. However, survival of P. noxius at deep root tissue is still unknown and should be studied in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:27:37Z (GMT). No. of bitstreams: 1 ntu-107-R04645012-1.pdf: 3480228 bytes, checksum: a5136bfc0957c0343686d5b64f01e026 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iv 圖目錄 viii 表目錄 ix 第一章 前言 1 第二章 前人研究 6 一、褐根病菌在田間罹病殘根之殘存能力 6 二、土壤含水率及淹水處理對於褐根病菌殘存之影響 6 三、化學防治對於褐根病菌殘存之影響 6 四、樹幹注射後病原菌於罹病組織中之殘存 8 第三章 材料與方法 9 一、褐根病供試菌株於馬拉巴栗之接種及殘存 9 (一) 褐根病供試菌株之來源及保存 9 (二) 褐根病供試菌株之接種源製備 9 (三) 褐根病供試菌株於馬拉巴栗幼苗之接種 9 (四) 褐根病供試菌株於馬拉巴栗組織之殘存 10 二、褐根病防治藥劑之篩選 11 三、以馬拉巴栗幼苗模擬預防性及治療性注射之測試 13 (一) 預防注射殺菌劑對於馬拉巴栗幼苗褐根病之防治效果 13 (二) 殺菌劑治療性注射對於馬拉巴栗幼苗褐根病之防治效果 13 (三) 藥劑注射對於馬拉巴栗幼苗組織中褐根病菌殘存之影響 14 四、土壤掩埋處理對於馬拉巴栗組織中褐根病菌殘存之影響 15 五、土壤掩埋處理對於田間殘根組織中褐根病菌殘存之影響 16 六、褐根病菌殘存之田間調查及樹幹注射對於褐根病菌殘存之影響 17 七、藥劑對於田間罹病樹頭、病區土壤及殘根中褐根病菌殘存之影響 24 第四章 實驗結果 27 一、褐根病供試菌株於馬拉巴栗之接種及殘存 27 (一) 褐根病供試菌株之來源及保存 27 (二) 褐根病供試菌株之接種源製備 29 (三) 褐根病供試菌株於馬拉巴栗幼苗之接種 30 (四) 褐根病供試菌株於馬拉巴栗組織之殘存 35 二、褐根病防治藥劑之篩選 37 三、利用馬拉巴栗幼苗模擬褐根病之治療性與預防性注射 39 (一) 殺菌劑預防性注射對於馬拉巴栗幼苗褐根病之防治效果 39 (二) 殺菌劑治療性注射對於馬拉巴栗幼苗褐根病之防治效果 42 (三) 藥劑預防注射對於馬拉巴栗幼苗組織中褐根病菌殘存之影響 45 四、土壤掩埋處理對於馬拉巴栗組織中褐根病菌殘存之影響 49 五、土壤掩埋處理對於田間殘根組織中褐根病菌殘存之影響 53 六、褐根病菌殘存之田間調查及樹幹注射對於褐根病菌存之影響 55 七、藥劑對於田間罹病樹頭及病土中褐根病菌殘存之影響 59 第五章 討論 60 參考文獻 70 | |
dc.language.iso | zh-TW | |
dc.title | 樹木褐根病菌在田間及發病組織殘存之研究 | zh_TW |
dc.title | Study on survival of Phellinus noxius in the field and diseased tissue | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 郭章信,楊秀珠,吳孟玲 | |
dc.subject.keyword | 褐根病,褐根病菌,殘存,藥劑注射,藥劑灌注,土壤改良劑, | zh_TW |
dc.subject.keyword | brown root rot disease,Phellinus noxius,survival,fungicides injection,fungicides drenching,soil amendment, | en |
dc.relation.page | 72 | |
dc.identifier.doi | 10.6342/NTU201803232 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
顯示於系所單位: | 植物醫學碩士學位學程 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 3.4 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。