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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張喜寧(Doris C. N., Chang) | |
dc.contributor.author | Po-Hung Wu | en |
dc.contributor.author | 吳柏宏 | zh_TW |
dc.date.accessioned | 2021-05-17T10:17:58Z | - |
dc.date.available | 2013-03-19 | |
dc.date.available | 2021-05-17T10:17:58Z | - |
dc.date.copyright | 2012-03-19 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2012-02-03 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7035 | - |
dc.description.abstract | 蘭花產業是我國農業發展的旗艦作物,在所有種類的蘭花中,蝴蝶蘭是世界上最重要的盆花作物之一。台灣是世界上最主要的蝴蝶蘭生產國家,2011年台灣的蝴蝶蘭外銷總值已經達到27億台幣。本論文針對蘭菌對於蝴蝶蘭的生長促進、誘導抗病、以及外加植物生長調節物質對於開花品質的增進以及開花時間的調節作了一系列詳細的研究,以期在學術上或實際的產業利用上,有所貢獻。利用蘭菌之接種(Rhizoctonia spp. (R02及R04))可有效促進蘭苗的生長,並且可增強對軟腐病菌(Erwinia chrysanthemi)的抗性。經由抑制性扣減雜合技術(Suppression subtractive hybridization,SSH)的方式,已找到數個可能與抗軟腐病相關的基因。在蝴蝶蘭開花控制時,施用三種不同濃度的三種cytokinins (N-6-benzyladenine(50、100、150 mg·L-1)、kinetin(100、200、300 mg·L-1)及2-isopentenyladenine(50、100、150 mg·L-1))。結果顯示施用N-6-benzyladenine 100及150 mg·L-1對開花的促進效果最佳,可顯著增加花梗數及花朵數。 | zh_TW |
dc.description.abstract | Orchid industry is the flagship of agricultural crop development in Taiwan. Phalaenopsis, in all type of orchids, is the most important potted flower in the world.
Taiwan is the main orchid producer countries in the world. In 2011 the export value of Taiwan's Phalaenopsis orchid has reached NT $ 2.7 billion. This thesis focus on the utility of rhizoctonia to phalanopsis including growth promoting, induce disease resistance, improve the quality of flowering and flowering time regulation. I expect these results can be contributed to the orchid industry and basic research in orchid. Inoculation of Rhizoctonia spp. (R02 and R04) can enhances Phalaenopsis orchid’s growth and resistance to Erwinia chrysanthemi. By suppression subtractive hybridization (SSH) assay, we found several candidate genes which play a role at against Erwinia chrysanthemi. In the other hand, application of three different concentrations of the three cytokinins (N-6-benzyladenine (50、100、150 mg·L-1)、kinetin (100、200、300 mg·L-1) and 2-isopentenyladenine (50、100、150 mg·L-1)) at flowering control. The results showed that application of 100 or 150 mg·L-1 N-6-benzyladenine on flowering for best results, can significantly increase the number and flower stalk number. | en |
dc.description.provenance | Made available in DSpace on 2021-05-17T10:17:58Z (GMT). No. of bitstreams: 1 ntu-100-D93628002-1.pdf: 3693640 bytes, checksum: 308cd56865696647b9e1a0e23af30f6c (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要………………………………………………………………III
英文摘要………………………………………………………………………IV 圖目錄…………………………………………………………………VII 表目錄…………………………………………………………………IX 前言……………………………………………………………………10 前人研究………………………………………………………………12 第一章、蝴蝶蘭菌根之效益…………………………………………39 摘要……………………………………………………………………39 第一節、蘭菌接種可促進蝴蝶蘭生長………………………………40 前言……………………………………………………………………40 材料與方法……………………………………………………………42 結果與討論……………………………………………………………44 結論……………………………………………………………………44 第一節圖表……………………………………………………………45 第二節、蘭菌接種可增加抗軟腐病能力……………………………48 前言……………………………………………………………………48 材料與方法……………………………………………………………49 結果與討論……………………………………………………………51 結論……………………………………………………………………52 第二節圖表……………………………………………………………53 第三節、接種蘭菌之蝴蝶蘭其生長抗病之機制探討………………59 前言……………………………………………………………………59 材料與方法……………………………………………………………61 結果與討論……………………………………………………………66 結論……………………………………………………………………69 第三節圖表……………………………………………………………70 參考文獻………………………………………………………………73 第二章、植物生長物質在蝴蝶蘭的生長與開花上之應用…………89 摘要……………………………………………………………………89 第一節、出瓶前涼溫處理及出瓶後施用甲殼素可促進蝴蝶蘭小苗生長………………………………………………………………………90 前言……………………………………………………………………90 材料與方法……………………………………………………………91 結果與討論……………………………………………………………93 結論……………………………………………………………………94 第一節圖表……………………………………………………………95 第二節、使用N-6-benzyladenine及Gibberelic acid 3調控蝴蝶蘭開花………………………………………………………………………101 前言……………………………………………………………………101 材料與方法……………………………………………………………103 結果與討論……………………………………………………………105 結論……………………………………………………………………107 第二節圖表……………………………………………………………108 第三節、使用N-6-benzyladenine、kinetin及2-isopentenyladenine調控蝴蝶蘭開花………………………………………………………113 前言……………………………………………………………………113 材料與方法……………………………………………………………114 結果與討論……………………………………………………………115 結論……………………………………………………………………116 參考文獻………………………………………………………………117 第三節圖表……………………………………………………………119 附錄(已發表之國際科學期刊三篇)…………………………………121 | |
dc.language.iso | zh-TW | |
dc.title | 蝴蝶蘭栽培技術之改良及菌根之應用 | zh_TW |
dc.title | Improvement of cultivation and the use of orchid mycorrhiza for phalaenopsis orchids | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 陳文輝,黃定鼎,王均琍,詹明才 | |
dc.subject.keyword | 蝴蝶蘭,蘭菌,抑制性扣減雜合技術,抗軟腐病,甲殼素,涼溫處理, | zh_TW |
dc.subject.keyword | Phalaenopsis,orchid micorrhizal fungi,suppression subtractive hybridization,anti soft rot disease,chitosan,cool temperature treatment, | en |
dc.relation.page | 135 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2012-02-06 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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