請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70278
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李雨(U Lei) | |
dc.contributor.author | Kuan-Yu Chen | en |
dc.contributor.author | 陳冠宇 | zh_TW |
dc.date.accessioned | 2021-06-17T04:25:10Z | - |
dc.date.available | 2021-08-19 | |
dc.date.copyright | 2018-08-19 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-14 | |
dc.identifier.citation | [1] K. Kurita, “Controlled functionalization of the polysaccharide chitin,” Progress in Polymer Science, vol. 26, pp. 1921-1971, 2001.
[2] C. K. S. Pillai, W. Paul, and C. P. Sharma, “Chitin and chitosan polymers: Chemistry, solubility and fiber formation,” Progress in Polymer Science, vol. 34, pp. 641-678, 2009. [3] T. Chandy, and C. P. Sharma, “Chitosan – as a biomaterial,” Biomaterials, Artificial Cells and Artificial Organs, vol. 18(1), pp. 1-24, 1990. [4] B. K. Park, and M. M. Kim, “Applications of chitin and its derivatives in biological medicine,” International Journal of Molecular Sciences, vol. 11, pp. 5152-5164, 2010. [5] M. Tracey, “Chitin, in: Modern Methods of Plant Analysis/Moderne Methoden der Pflanzenanalyse,” Springer, pp. 264-274, 1955. [6] N. K. Mathur, C. K. Narang, “Chitin and chitosan, versatile polysaccharides from marine animals,” Journal of Chemical Education, vol. 67, pp. 938, 1990. [7] K. H. Meyer, and G. W. Pankow, Helvetica Chimica Aceta, vol. 18, pp. 589, 1935. [8] J. Blackwell, “Physical methods for the determination of chitin structure and conformation,” In Biomass, Part B, Lignin, Pectin, and chitin, Academic Press, San Diego, pp. 435-442, 1988. [9] M. Dash, F. Chiellini, R. M. Ottenbrite, and E. Chiellini, “Chitosan - A versatile semi-synthetic polymer in biomedical applications,” Progress in Polymer Science, vol. 36, pp. 981-1014, 2011. [10] H. Yi, L. Q. Wu, W. E. Bentley, R. Ghodssi, G. W. Rubloff, J. N. Culver, and G. F. Payne, “Biofabrication with chitosan,” Biomacromolecules, vol. 6, pp. 2881-2894, 2005 [11] T. Dai, M. Tanaka, Y. Y. Huang, and M. R. Hamblin, “Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects,” Expert Review of Anti-infective Therapy, vol. 9, pp. 857-879, 2011. [12] A. E. Pusateri, J. B. Holcomb, B. S. Kheirabadi, H. B. Alam, C. E. Wade, and K. L. Ryan, “Making sense of the preclinical literature on advanced hemostatic products,” The Journal of Trauma Injury Infection and Critical Care, vol. 60, pp. 674–682, 2006. [13] S. D. Gordy, P. Rhee, and M. A. Schreiber, “Military applications of novel hemostatic devices,” Expert Review of Medical Devices, vol. 8(1), pp. 41-47, 2011. [14] P. R. Klokkevold, H. Fukayama, E. C. Sung, and C. N. Bertolami, “The Effect of Chitosan (poly-NAcetylGlucosamine) on Lingual Hemostasis in Heparinized Rabbits,” Journal of Oral and Maxillofacial Surgery, vol. 57, pp. 49-52, 1999. [15] R. Millner, A. S. Lockhart, and R. Marr, “Chitosan arrests bleeding in major hepatic injuries with clotting dysfunction: an in vivo experimental study in a model of hepatic injury in the presence of moderate systemic heparinisation,” The Annals of The Royal College of Surgeons of England, vol. 92, pp. 559–561, 2010. [16] B. L. Bennett, L. F. Littlejohn, B. S. Kheirabadi, F. K. Butler, R. S. Kotwal, M.A. Dubick, and J. A. Bailey, “Management of external hemorrhage in tactical combat casualty care: Chitosan-based hemostatic gauze dressings,” TCCC Guidelines - Change 13-05, Journal of Special Operations Medicine, vol. 14(3), pp. 40-57, 2014. [17] M. A. Brown, M. R. Daya, and J. A. Worley, “Experience with chitosan dressings in a civilian EMS system,” The Journal of Emergency Medicine, vol. 37(1), pp. 1-7, 2009. [18] N. Nguyen, S. Hasan, L. Caufield, F. S. Ling, and C. R. Narins, “Randomized controlled trial of topical hemostasis pad use for achieving vascular hemostasis following percutaneous coronary intervention,” Catheterization and Cardiovascular Interventions, vol. 69(6), pp. 801-807, 2007. [19] L. Littlejohn, M. D., B. L. Bennett, and B. Drew, “Application of Current Hemorrhage Control Techniques for Backcountry Care: Part Two, Hemostatic Dressings and Other Adjuncts,” Wilderness & Environmental Medicine, vol. 26, pp. 246–254, 2015 [20] T. C. Chou, E. Fu, C. J. Wu, and J. H. Yeh, “Chitosan enhances platelet adhesion and aggregation,” Biochemical and Biophysical Research Communications, vol. 302, pp. 480–483, 2003. [21] H. S. Thatte, S. Zagarins, S. F. Khuri, and T. H. Fischer, “Mechanisms of Poly-N-Acetyl Glucosamine Polymer–Mediated Hemostasis: Platelet Interactions,” Journal of Trauma and Acute Care Surgery, vol. 57, pp. S13–S21, 2004. [22] J. Jesty, M. Wieland, and J. Niemiec, “Assessment in vitro of the active hemostatic properties of wound dressings,” Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 89, pp. 536–542, 2009. [23] A. Ashkin, “Acceleration and Trapping of Particles by Radiation Pressure,” Phys. Rev. Lett., vol. 24, Issue 4, pp. 156-159, 1970. [24] A. Ashkin, “Acceleration and Trapping of Particles by Radiation Pressure,” Phys. Rev. Lett., vol. 24, Issue 4, pp. 156-159, 1970. [25] A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, “Observation of a single-beam gradient force optical trap for dielectric particles,” Optics Letters, vol. 11, pp. 288–290, 1986. [26] A. Ashkin, J. M. Dziedzic, and T. Yamane, “Optical trapping and manipulation of single cells using infra-red laser beams,” Nature 330, pp. 769-771, 1987. [27] A. Ashkin, and J. M. Dziedzic, “Optical trapping and manipulation of viruses and bacteria,” Science, vol. 235, Issue 4795, pp. 1517-1520, 1987. [28] A. Ashkin, and J. M. Dziedzic, “Internal cell manipulation using infrared laser traps,” PNAS, vol. 86, pp. 7914-7922, 1989. [29] Y. Tadir, W. H. Wright, O. Vafa, T. Ord, R. H. Asch, and M. W. Berns, “Micromanipulation of sperm by a laser generated optical trap,” Fertil. Steril. 52, pp. 870-873, 1989. [30] S. Seeger, S. Monajembashi, K. J. Hutter, G. Futterman, J. Wolfrum, and K. O. Greulich, “Application of laser optical tweezers in immunology and molecular genetics,” Cytometry 12, pp. 497-504, 1991. [31] Steven Chu, “Laser Cooling and Manipulation of Atoms and Selected Applications,” Enrico Fermi, pp. 239-288, 1992. [32] A. Ashkin, “Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Biophys. J., vol. 61, pp. 569-582, 1992. [33] A. Constable, Jinha Kim, J. Mervis, F. Zarinetchi, and M. Prentiss, “Demonstration of a Fiber-Optical Light-Force Trap,” Optics Letters, vol. 18, No. 21, 1993. [34] P. J. Rodrigo, R. L. Eriksen, V. R. Daria, and J. Glückstad, “Interactivelight-driven and parallel manipulation of inhomogeneous particles,” Optics Express, vol. 10, No. 26, pp. 1550-1556, 2002. [35] M. Ozkan, M. Wang, C. Ozkan, R. Flynn, A. Birkbeck, and S. Esener, “Optical manipulation of objects and biological cells in microfluidic devices,” Biomedical Microdevice, vol. 5, pp. 61-67, 2003. [36] J. Enger, M. Goksör, K. Ramser, P. Hagberg, and D. Hanstorp, “Optical tweezers applied to a microfluidic system,” Lab on a Chip, vol. 4, pp. 196-200, 2004. [37] K. C. Neuman, and S. M. Block, “Optical trapping,” Review of Scientific Instruments, vol. 75, pp. 2787–2809, 2004. [38] Y. J. Lo, U. Lei, K. Y. Chen, Y. Y. Lin, C. C. Huang, M. S. Wu, and P. C. Yang, “Derivation of the cell dielectric properties based on Clausius-Mossotti factor,” Applied Physics Letters, vol. 104, 113702, 2014. [39] G. Sagvolden, I. Giaever, E. O. Pettersen, and J. Feder, “Cell adhesion force microscopy” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, pp. 471–476, 1999. [40] V. M. Correlo, E. D. Pinho, I. Pashkuleva, M. Bhattacharya, N. M. Neves, and R. L. Reis, “Water absorption and degradation characteristics of chitosan-based polyesters and hydroxyapatite composites,” Macromolecular Bioscience, vol. 7, pp. 354–363, 2007. [41] J. Happel, and H. Brenner, “Low Reynolds Number Hydrodynamics,” Martinus Nijhoff Publishers, Boston, 1986. [42] E. L. Florin, V. T. Moy, and H. E. Gaub, “Adhesion forces between individual ligand-receptor pairs,” Science, vol. 264, pp. 415–417, 1994. [43] A. Fontes, H. P. Fernandes, A. A. Thomaz, L. C. Barbosa, M. L. Barjas-Castro, and C. L. Cesar, “Measuring electrical and mechanical properties of red blood cells with double optical tweezers,” Journal of Biomedical Optics, vol. 13, 014001, 2008. [44] B. W. David, “Introductory Chemistry,” FlatWorld, 2011. [45] K. Kendall, and A. D. Roberts, “van der Waals forces influencing adhesion of cells,” Philosophical Transactions of the Royal Society B, vol.370, 20140078, 2015. [46] H. P. Fernandes, C. L. Cesar, and M. L. Barjas-Castro, “Electrical properties of the red blood cell membrane and immunohematological investigation,” Rev Bras Hematol Hemoter, vol. 33(4), pp. 297–301, 2011. [47] C. Wagner, P. Steffen, and S. Svetina, “Aggregation of red blood cells: From rouleaux to clot formation,” Comptes Rendus Physique, vol. 14, pp. 459–469, 2013. [48] J. N. Israelachvilli, “Intermolecular and Surface Forces 3rd Edition,” Academic Press, London, 2011. [49] R. J. Hunter, “Zeta potential in colloid science: principles and applications,” Academic press, London, 1981. [50] F. Tokumasu, G. R. Ostera, C. Amaratunga, and R. M. Fairhurst, “Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection,” Experimental Parasitology, vol. 131, pp. 245-251, 2012. [51] E. H. Eylar, M. A. Madoff, O. V. Brody, and J. L. Oncley, “The contribution of sialic acid to the surface charge of the erythrocyte,” Journal of Biological Chemistry, vol. 237, pp. 1992–2000, 1962. [52] K. M. Jan, and S. Chien, “Role of surface electric charge in red blood cell interaction,” Journal of General Physiology, vol. 61, pp. 638–654, 1973. [53] F. Tokumasu, G. R. Ostera, C. Amaratunga, and R. M. Fairhurst, “Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection,” Experimental Parasitology, vol. 131, pp. 245–251, 2012. [54] M. L. Turgeon, “Clinical Hematology: Theory and procedures 4th Edition,” Lippincott Williams & Wilkins, Baltimore, 2004. [55] K. M. Jan, and S. Chien, “Influence of the ionic composition of fluid medium on red cell aggregation,” Journal of General Physiology, vol. 61, pp. 655–668, 1973. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70278 | - |
dc.description.abstract | 幾丁聚醣所製成的紗布,其止血性能優於一般傳統使用的紗布,一般認為是因為幾丁聚醣帶正電荷,吸引帶負電荷的紅血球黏附而形成黏膜屏蔽,阻擋了血液由傷口流出,達到止血的目的,但詳細的原因並未被解釋,本研究的第一個目標,便是從力學的角度去探討相關的止血機制。幾丁聚醣紗布在具有凝血功能障礙的動物實驗中,仍能產生止血的功能,但動物實驗耗費的資源龐大,因此本研究的第二個目標為:在生物體外的環境中,提供能定量量測止血性能的環境與裝置。
目前量測不同紗布敷料的止血性能多為靜態測試,若能在流動的環境中進行止血測量,其量測結果會更貼近於真實的情形;Jesty等人(2009)提出一套流體裝置(此稱為CPG裝置),是利用恆定的壓力梯度,讓流體通過受測紗布後,依照所收集到的流體質量來評估紗布的止血性能,此裝置能以動態的方式進行實驗,但需要大量的測試液體,本研究的第三個目標:提出一個可使用較少測試液的替代實驗裝置。 本研究完成了三項工作: 1. 利用定流量(CFR)裝置量測各種紗布敷料的止血性能,以恆定的流動速率推動測試液體使其通過紗布敷料,觀察與記錄紗布兩側的液體壓力降,壓力降越大則表示紗布敷料的止血性能越佳,實驗結果與CPG裝置測量的結果相符合,但是CFR裝置消耗較少的測試液,而CPG裝置模擬出血的情況更為真實。 2. 利用定流量裝置以全血與洗滌紅血球液(即除去血小板與凝血因子)進行實驗,結果顯示幾丁聚醣的紗布在兩種血液中皆有優異的止血效果,由此可知,紅血球黏附於幾丁聚醣而達到止血的方式,是獨立於典型的人體凝血機制,即使沒有血小板與凝血因子的參與作用,仍能達到止血的 功效。 3. 架設一套光鉗系統,量測紅血球細胞在各種紗布纖維上的黏附力,量測到的黏附力約為3.82 pN,因為紅血球黏附於紗布纖維的力量太小,所以此黏附現象並不是造成止血的唯一原因。 然而,無論在靜態或是流動的環境下,可以觀察到紅血球細胞聚集於幾丁聚醣紗布纖維旁,並且堆疊排列成層狀結構,但並未發生於其它的紗布纖維上,此層狀結構是形成黏膜屏蔽的初始跡象,而後達到止血的目的。紅血球細胞聚集的原因,可透過量測血液與幾丁聚醣粉末的混合液體的界達電位(zeta potential)與pH值來解釋,由DLVO (Derjaguin-Landau-Verwey-Overbeek)理論得知,幾丁聚醣去質子化所釋出的H+離子與紅血球細胞膜上的COO¯離子相結合,使得紅血球彼此間相斥的電雙層力減弱而形成多層排列結構,又因幾丁聚醣的良好吸水性,增加了傷口附近的血液黏度,進而使血液的流量減少,再加上按壓於傷口的反向壓力,各個因素造就出優異的止血效果。本研究的結果有利於設計出提升止血效果的幾丁聚醣紗布,並可應用於一般生物醫學。 | zh_TW |
dc.description.abstract | Chitosan-based wound dressings are superior to traditional dressings for hemostatsis, and can arrest bleeding with clotting dysfunction. It is generally claimed, but without detailed reasoning, that “the chitosan cross-links red blood cells (RBCs) to form mucoadhesive barrier” to block the bleeding. The first goal of this study is to understand the related mechanism from a mechanical view point.
Hemostatsis succeeds using chitosan under clotting dysfunction was supported qualitatively by animal tests, but quantitative measurement was still lacking. The second goal of this study is provide such a quantitative result in an in vitro environment. Static tests were employed mostly for the hemostatic performance tests of various dressings, but it would be more realistic if the test was performed in a flowing environment. A flow-through device (called the CPG device here) was proposed by Jesty et al. (2009) under a constant pressure gradient, and the performance was assessed via measuring the amount of fluid masses through the device. However, a substantial amount of test fluid is required for the testing using the CPG device. The third goal of this study is to propose an alternative device using less test fluid. Three works were accomplished in this study. First, a flow-through device under a constant flow rate (called the CFR device) was proposed and demonstrated successfully for assessing dynamically the hemostatic performance of various wound dressings via comparing the testing using the CPG device. The pressure drops across the dressings are measured for assessing the performance in the CFR device. The CFR device consumes less test fluid, but the CPG device models more realistic the scenario of bleeding. Secondly, detailed experiments were performed using the CFR device with both the human whole blood and washed blood (with clotting factors and platelets removed) as test fluids, and the results agree with each other within experimental errors. Such quantitative findings show definitely that the hemostatic enhancement due to chitosan is independent of classical clotting pathways. Thirdly, the adhesion forces of red blood cells on various yarns were measured using an optical tweezers. The adhesion force is small, around 3.83 pN, such that the direct adhesion cannot be the sole cause for hemostasis. However, it was observed that layer structures of aggregated RBCs were formed next to chitosan objects in both static and flowing environments, but not formed next to other yarns. Such a layer structure is the clue for the initiation of the mucoadhesive barrier, and thus hemostatsis. Through the supporting measurements of zeta potentials of RBCs and pH’s using blood-chitosan mixtures, it is proposed here that the formation of the RBC layer structures next to chitosan objects is due to the reduction of repulsive electric double layer force between RBCs, because of the association of H+ deprotonated from chitosan with COO on RBC membrane, under the DLVO (Derjaguin-Landau-Verwey-Overbeek) theory. The results are beneficial for designing effective chitosan-based wound dressings, and also for general biomedical applications. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:25:10Z (GMT). No. of bitstreams: 1 ntu-107-D98543006-1.pdf: 4995798 bytes, checksum: 06f0d5e74e8334447fb0ac0f9058d495 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 致謝 I
摘要 II Abstract IV 目錄 VI 圖表目錄 VIII 第一章 緒論 1 1-1 導言與文獻回顧 1 1-2 研究動機與目的 10 1-3 本文架構 11 第二章 實驗方法與設備 13 2-1 PDMS圓柱體於微流道中的實驗 13 2-2 定壓力梯度裝置的設計與製作 15 2-3 定流量裝置的設計與製作 17 2-3-1 流道設計 18 2-3-2 PDMS流道晶片翻模製程 19 2-3-3 PDMS晶片結合 19 2-4 實驗紗布 20 2-5 實驗血液樣本 23 2-6 光鉗系統架設 24 2-7 界達電位、pH值與黏度的量測 37 第三章 實驗結果與討論 39 3-1 定壓力梯度裝置的實驗結果 39 3-2 定流量裝置的實驗結果 44 3-3 以光鉗系統量測紅血球與各種纖維間的黏附力 50 3-4 纖維及紗布上紅血球黏附堆疊的現象 55 3-5 幾丁聚醣紗布止血機制的扼要歸納及其應用 73 第四章 結論與未來展望 75 4-1 結論 75 4-2 未來展望 76 參考文獻 77 | |
dc.language.iso | zh-TW | |
dc.title | 幾丁聚醣傷口敷料止血性能的力學特性 | zh_TW |
dc.title | Mechanical Aspects of the Hemostatic Characteristics of Chitosan-Based Wound Dressings | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 沈弘俊(Horn-Jiunn Sheen),胡文聰(Andrew Wo),楊政穎(Cheng-Ying Yang),林子忻(Tzu-Hsin Lin),郭雅雯(Ya-Wen Kuo) | |
dc.subject.keyword | 幾丁聚醣,紗布敷料,止血性能,力學,流量裝置, | zh_TW |
dc.subject.keyword | Chitosan,Wound dressings,Hemostatsis,Mechanics,Flow-through device, | en |
dc.relation.page | 83 | |
dc.identifier.doi | 10.6342/NTU201802379 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-15 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 4.88 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。