請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70252
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳穎練 | |
dc.contributor.author | Yu-Hsin Lai | en |
dc.contributor.author | 賴于歆 | zh_TW |
dc.date.accessioned | 2021-06-17T04:24:49Z | - |
dc.date.available | 2021-08-18 | |
dc.date.copyright | 2018-08-18 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-15 | |
dc.identifier.citation | 1. Adaskaveg, J., and Förster, H. 2009. New developments in postharvest fungicide registrations for edible horticultural crops and use strategies in the United States. Springer, Dordrecht, Netherlands. pp. 107-117.
2. Agtarap, A., Chamberlin, J. W., Pinkerton, M., and Steinrauf, L. K. 1967. Structure of monensic acid, a new biologically active compound. Journal of the American Chemical Society 89:5737-5739. 3. Arentshorst, M., Niu, J., and Ram, A. F. 2015. Efficient generation of Aspergillus niger knock out strains by combining NHEJ mutants and a split marker approach. Pages 263-272 in: Genetic Transformation Systems in Fungi, Volume 1. Springer. 4. Atkinson, K. D., Jensen, B., Kolat, A. I., Storm, E. M., Henry, S. A., and Fogel, S. 1980. Yeast mutants auxotrophic for choline or ethanolamine. Journal of bacteriology 141:558-564. 5. Azor, M., Gene, J., Cano, J., and Guarro, J. 2007. Universal in vitro antifungal resistance of genetic clades of the Fusarium solani species complex. Antimicrobial Agents and Chemotherapy 51:1500-1503. 6. Bakovic, M., Fullerton, M. D., and Michel, V. 2007. Metabolic and molecular aspects of ethanolamine phospholipid biosynthesis: the role of CTP: phosphoethanolamine cytidylyltransferase (Pcyt2). Biochemistry and Cell Biology 85:283-300. 7. Bapat, P., Nandy, S. K., Wangikar, P., and Venkatesh, K. 2006. Quantification of metabolically active biomass using methylene blue dye reduction test (MBRT): measurement of CFU in about 200 s. Journal of Microbiological Methods 65:107-116. 8. Baricevic, D., and Bartol, T. 2000. The biological/pharmacological activity of the Salvia genus. The Genus Salvia. Harwood Academic Publishers, Taylor & Francis e-Library. pp. 9. Bennett, F. 1928. On two species of Fusarium, F. culmobum (W. G. Sm.) Sacc. and F. avenaceum (Fries.) Sacc., as parasites of cereals. Annals of Applied Biology 15:213-244. 10. Beran, M., and Zima, J. 1993. Determination of monensins A and B in the fermentation broth of Streptomyces cinnamonensis by high performance liquid chromatography. Chromatographia 35:206-208. 11. Blancard, D. 2018. Tomato Diseases: Identification, Biology and Control: A Colour Handbook. 2 ed. CRC Press, USA. pp. 320-324. 12. Bosscha, M. I., van Dissel, J. T., Kuijper, E. J., Swart, W., and Jager, M. J. 2004. The efficacy and safety of topical polymyxin B, neomycin and gramicidin for treatment of presumed bacterial corneal ulceration. British Journal of Ophthalmology 88:25-28. 13. Brown, N. A., Urban, M., Van de Meene, A. M., and Hammond-Kosack, K. E. 2010. The infection biology of Fusarium graminearum: Defining the pathways of spikelet to spikelet colonisation in wheat ears. Fungal Biology 114:555-571. 14. Chand, R., and Srinivasan, R. 1979. Extraction & fractionation of lipids (polar & non-polar) from Fusarium sp. Indian Journal of Experimental Biology 17:66-68. 15. Chang, D. C., Grant, G. B., O'Donnell, K., Wannemuehler, K. A., Noble-Wang, J., Rao, C. Y., . . . Park, B. J. 2006. Multistate outbreak of Fusarium keratitis associated with use of a contact lens solution. The Journal of the American Medical Association 296:953-963. 16. Chao, C. P. 2008. Panama disease, Illustrated Handbook of Plant Protection Series 18 (香蕉黃葉病-植物保護圖鑑系列18). Bureau of Animal and Plant Health Inspection and Quarantine, COA, Executive Yuan, Taipei, Taiwan (In Chinese). pp. 17. Chen, Y. L., Montedonico, A. E., Kauffman, S., Dunlap, J. R., Menn, F. M., and Reynolds, T. B. 2010. Phosphatidylserine synthase and phosphatidylserine decarboxylase are essential for cell wall integrity and virulence in Candida albicans. Molecular Microbiology 75:1112-1132. 18. Chen, Y. Y., Lin, T. C., Chung, W. H., and Wang, C. L. 2015. Screening antogonistic microorganisms that inhibit chlamydospore germination of Fusarium oxysporum f. sp. lycopersici. Plant Pathology Bulletin 24:251-266. 19. Chung, P.-C., and Wu, T.-Y. 2017. The management of strawberry Fusarium wilt during soil leisure period. Research Bulletin of Miaoli District Agricultural Research and Extension Station 6:15-25. (In Chinese). 20. Clinical and Laboratory Standards Institute (CLSI). 2008. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous fungi; Approved Standard—Second Edition, CLSI Document M38-A2. CLSI, Wayne, Pennsylvania, USA. pp. 21. Coleman, J. J., Rounsley, S. D., Rodriguez-Carres, M., Kuo, A., Wasmann, C. C., Grimwood, J., . . . Vanetten, H. D. 2009. The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion. PLoS Genetics 5:e1000618. 22. Cuomo, C. A., Guldener, U., Xu, J. R., Trail, F., Turgeon, B. G., Di Pietro, A., . . . Kistler, H. C. 2007. The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science 317:1400-1402. 23. da Silva Ferreira, M. E., Kress, M. R., Savoldi, M., Goldman, M. H. S., Härtl, A., Heinekamp, T., . . . Goldman, G. H. 2006. The akuBKU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryotic Cell 5:207-211. 24. Davis, S. E., Tams, R. N., Solis, N., Wagner, A. S., Chen, T., Jackson, J. W., . . . Sparer, T. E. 2018. Candida albicans cannot acquire sufficient ethanolamine from the host to support virulence in the absence of de novo phosphatidylethanolamine synthesis. Infection and Immunity:IAI. 00815-00817. 25. Debono, M., Willard, K. E., Kirst, H. A., Wind, J. A., Crouse, G. D., Tao, E. V., . . . Ose, E. E. 1989. Synthesis and antimicrobial evaluation of 20-deoxo-20-(3,5-dimethylpiperidin-1-yl)desmycosin (tilmicosin, EL-870) and related cyclic amino derivatives. The Journal of Antibiotics 42:1253-1267. 26. Di Bartolomeo, F., Wagner, A., and Daum, G. 2017. Cell biology, physiology and enzymology of phosphatidylserine decarboxylase. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids 1862:25-38. 27. Di Bartolomeo, F., Wagner, A., and Daum, G. 2017. Cell biology, physiology and enzymology of phosphatidylserine decarboxylase. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 1862:25-38. 28. Dyakov, Y., Dzhavakhiya, V., and Korpela, T. 2007. Comprehensive and molecular phytopathology. Elsevier. pp. 421-465. 29. Essarioui, A., LeBlanc, N., Kistler, H. C., and Kinkel, L. L. 2017. Plant community richness mediates inhibitory interactions and resource competition between Streptomyces and Fusarium populations in the rhizosphere. Microbial Ecology 74:157-167. 30. Fang, X., You, M. P., and Barbetti, M. J. 2012. Reduced severity and impact of Fusarium wilt on strawberry by manipulation of soil pH, soil organic amendments and crop rotation. European Journal of Plant Pathology 134:619-629. 31. Folch, J., Lees, M., and Sloane Stanley, G. H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. The Journal of Biological Chemistry 226:497-509. 32. Frandsen, R. J., Rasmussen, S. A., Knudsen, P. B., Uhlig, S., Petersen, D., Lysøe, E., . . . Larsen, T. O. 2016. Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin. Scientific Reports 6:26206. 33. Fuller, K. K., Chen, S., Loros, J. J., and Dunlap, J. C. 2015. Development of the CRISPR/Cas9 system for targeted gene disruption in Aspergillus fumigatus. Eukaryotic Cell 14:1073-1080. 34. Fuller, K. K., Chen, S., Loros, J. J., and Dunlap, J. C. 2015. Development of the CRISPR/Cas9 system for targeted gene disruption in Aspergillus fumigatus. Eukaryotic Cell:EC. 00107-00115. 35. Gachango, E., Hanson, L. E., Rojas, A., Hao, J. J., and Kirk, W. W. 2012. Fusarium spp. causing dry rot of seed potato tubers in Michigan and their sensitivity to fungicides. Plant Disease 96:1767-1774. 36. Gaspar, M., Pollero, R., and Cabello, M. 1994. Triacylglycerol consumption during spore germination of vesicular-arbuscular mycorrhizal fungi. Journal of the American Oil Chemists’ Society 71:449. 37. Grallert, B., Nurse, P., and Patterson, T. E. 1993. A study of integrative transformation in Schizosaccharomyces pombe. Molecular and General Genetics MGG 238:26-32. 38. Gravelat, F. N., Askew, D. S., and Sheppard, D. C. 2012. Targeted gene deletion in Aspergillus fumigatus using the hygromycin-resistance split-marker approach. Pages 119-130 in: Host-Fungus Interactions. Springer. 39. Greenberg, M. L., and Lopes, J. M. 1996. Genetic regulation of phospholipid biosynthesis in Saccharomyces cerevisiae. Microbiological Reviews 60:1. 40. Hankin, L., and Anagnostakis, S. 1975. The use of solid media for detection of enzyme production by fungi. Mycologia:597-607. 41. Hankin, L., and Anagnostakis, S. L. 1977. Solid media containing carboxymethylcellulose to detect Cx cellulase activity of micro-organisms. Microbiology 98:109-115. 42. Hanson, L. E., Schwager, S. J., and Loria, R. 1996. Sensitivity to thiabendazole in Fusarium species associated with dry rot of potato. Phytopathology 86:378-384. 43. Harris, S. D., Morrell, J. L., and Hamer, J. E. 1994. Identification and characterization of Aspergillus nidulans mutants defective in cytokinesis. Genetics 136:517-532. 44. Hassan, Y. I., and Bullerman, L. B. 2009. Wheat bran as an alternative substrate for macroconidia formation by some Fusarium species. Journal of microbiological methods 77:134-136. 45. Hatsch, D., Phalip, V., Petkovski, E., and Jeltsch, J.-M. 2006. Fusarium graminearum on plant cell wall: no fewer than 30 xylanase genes transcribed. Biochemical and Biophysical Research Communications 345:959-966. 46. He, Z., Zhang, J., Shi, X.-H., Hu, L.-L., Kong, X., Cai, Y.-D., and Chou, K.-C. 2010. Predicting drug-target interaction networks based on functional groups and biological features. PloS One 5:e9603. 47. Helmschrott, C., Sasse, A., Samantaray, S., Krappmann, S., and Wagener, J. 2013. Upgrading fungal gene expression on demand: improved systems for doxycycline-dependent silencing in Aspergillus fumigatus. Applied and Environmental Microbiology 79:1751-1754. 48. Herrmann, J., Fayad, A. A., and Müller, R. 2017. Natural products from myxobacteria: novel metabolites and bioactivities. Natural Product Reports 34:135-160. 49. Hikiji, T., Miura, K., Kiyono, K., Shibuya, I., and Ohta, A. 1988. Disruption of the CHO1 gene encoding phosphatidylserine synthase in Saccharomyces cerevisiae. The Journal of Biochemistry 104:894-900. 50. Hill, T. W., and Kafer, E. 2001. Improved protocols for Aspergillus minimal medium: trace element and minimal medium salt stock solutions. Fungal Genetics Reports 48:20-21. 51. Hou, Z., Xue, C., Peng, Y., Katan, T., Kistler, H. C., and Xu, J.-R. 2002. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Molecular Plant-Microbe Interactions 15:1119-1127. 52. Hsu, L.-H., Wang, H.-F., Sun, P.-L., Hu, F.-R., and Chen, Y.-L. 2017. The antibiotic polymyxin B exhibits novel antifungal activity against Fusarium species. International Journal of Antimicrobial Agents 49:740-748. 53. Hsu, S., and Lockwood, J. 1973. Chlamydospore formation in Fusarium in sterile salt solutions. Phytopathology 63:597-602. 54. Huang, C. N., Cornejo, M. J., Bush, D. S., and Jones, R. L. 1986. Estimating viability of plant-protoplasts using double and single staining. Protoplasma 135:80-87. 55. Huang, J. W., and Sun, S. K. 1982. Tomato wilt of Fusarium oxysporum (Schl.) f. sp. lycopersici (Sacc.) Snyder and Hansan, in Taiwan. Plant Protection Bulletin 24:265-270. 56. International Union of Pure and Applied Chemistry (IUPAC). 1997. Auxotrophy. Compendium of Chemical Terminology. 2 (the 'Gold Book') ed. pp. 57. Jonkers, W., Rodrigues, C. D. A., and Rep, M. 2009. Impaired colonization and infection of tomato roots by the Δfrp1 mutant of Fusarium oxysporum correlates with reduced CWDE gene expression. Molecular Plant-Microbe Interactions 22:507-518. 58. Jonkers, W., Dong, Y., Broz, K., and Kistler, H. C. 2012. The Wor1-like protein Fgp1 regulates pathogenicity, toxin synthesis and reproduction in the phytopathogenic fungus Fusarium graminearum. PLoS Pathogens 8:e1002724. 59. Katz, L., and Baltz, R. H. 2016. Natural product discovery: past, present, and future. Journal of Industrial Microbiology and Biotechnology 43:155-176. 60. Kikot, G. E., Hours, R. A., and Alconada, T. M. 2009. Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: a review. Journal of Basic Microbiology 49:231-241. 61. Kinch, M. S., Haynesworth, A., Kinch, S. L., and Hoyer, D. 2014. An overview of FDA-approved new molecular entities: 1827-2013. Drug Discovery Today 19:1033-1039. 62. Klaasen, J. A., and Nelson, P. E. 1996. Identification of a mating population, Gibberella nygamai sp. nov., within the Fusarium nygamai anamorph. Mycologia:965-969. 63. Klittich, C., and Leslie, J. F. 1988. Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi). Genetics 118:417-423. 64. Kwolek-Mirek, M., and Zadrag-Tecza, R. 2014. Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Research 14:1068-1079. 65. Leplat, J., Friberg, H., Abid, M., and Steinberg, C. 2013. Survival of Fusarium graminearum, the causal agent of Fusarium head blight. A review. Agronomy for Sustainable Development 33:97-111. 66. Leslie, J. F., and Summerell, B. A. 2006. The Fusarium laboratory manual. 1st ed. Blackwell Pub., Ames, Iowa. pp. 176-250. 67. Lewis, J. S., and Graybill, J. R. 2008. Fungicidal versus Fungistatic: what's in a word? Expert Opinion on Pharmacotherapy 9:927-935. 68. Li, C., Zhang, Y., Wang, H., Chen, L., Zhang, J., Sun, M., . . . Wang, C. 2018. The PKR regulatory subunit of protein kinase A (PKA) is involved in the regulation of growth, sexual and asexual development, and pathogenesis in Fusarium graminearum. Molecular Plant Pathology 19:909-921. 69. Lin, C.-Y., Ann, P.-J., Chang, C. A., Lo, C.-T., and Hsieh, T.-F. 2004. Non-pesticide Control of Crop Diseases. Taiwan Agricultural Research Institute Series 110:1-20. 70. Lin, H.-S., Kuo, C.-C., and Chen, H.-B. 2013. Activating fallow land─wheat. Taichung Agriculture News Bulletin (台中區農業專訊) 80:7-10. (In Chinese). 71. Lin, J. Y. 1995. Biological control and sustainable agriculture. Silkworm Apiculture News Bulletin (蠶蜂業專訊) 13. (In Chinese). 72. Lin, X., Alspaugh, J. A., Liu, H., and Harris, S. 2014. Fungal morphogenesis. Cold Spring Harbor perspectives in medicine:a019679. 73. Lin, Y.-H., Su, C.-C., Chao, C.-P., Chen, C.-Y., Chang, C.-J., Huang, J.-W., and Chang, P.-F. L. 2013. A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology 135:395-405. 74. Liu, E. C., Han, C. S., Hseih, S. H., Wang, S. S., R. H. Wang, and Chen, N. C. 2009. Breeding of a new cherry tomato variety “Tainan ASVEG No. 19”. Research Bulletin of Tainan District Agricultural Research and Extension Station 53:12-23. 75. Loponen, J., Sontag-Strohm, T., Venalainen, J., and Salovaara, H. 2007. Prolamin hydrolysis in wheat sourdoughs with differing proteolytic activities. Journal of Agricultural and Food Chemistry 55:978-984. 76. Low, B. K. 2001. Auxotroph. Encyclopedia of Genetics. 1 ed. Academic Press, USA. pp. 133. 77. Lowicki, D., and Huczynski, A. 2013. Structure and antimicrobial properties of monensin A and its derivatives: summary of the achievements. BioMed Research International. 78. Lysøe, E., Pasquali, M., Breakspear, A., and Kistler, H. C. 2011. The transcription factor FgStuAp influences spore development, pathogenicity, and secondary metabolism in Fusarium graminearum. Molecular Plant-Microbe Interactions 24:54-67. 79. Ma, L. J., van der Does, H. C., Borkovich, K. A., Coleman, J. J., Daboussi, M. J., Di Pietro, A., . . . Rep, M. 2010. Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature 464:367-373. 80. Madhavi, G. B., and Bhattiprolu, S. 2011. Evaluation of fungicides, soil amendment practices and bioagents against Fusarium solani-causal agent of wilt disease in Chilli. Journal of Horticultural Science 6:141-144. 81. Maier, F. J., Malz, S., Lösch, A. P., Lacour, T., and Schäfer, W. 2005. Development of a highly efficient gene targeting system for Fusarium graminearum using the disruption of a polyketide synthase gene as a visible marker. FEMS Yeast Research 5:653-662. 82. Marasas, W. F., Rheeder, J. P., Lamprecht, S. C., Zeller, K. A., and Leslie, J. F. 2001. Fusarium andiyazi sp. nov., a new species from sorghum. Mycologia:1203-1210. 83. Matějů, J., Karnetová, J., Nohýnek, M., and Vaněk, Z. 1988. Propionate and the production of monensins in Streptomyces cinnamonensis. Folia Microbiologica 33:440. 84. Matsuo, Y., Fisher, E., Patton-Vogt, J., and Marcus, S. 2007. Functional characterization of the fission yeast phosphatidylserine synthase gene, pps1, reveals novel cellular functions for phosphatidylserine. Eukaryotic cell 6:2092-2101. 85. Medh, J. D., and Weigel, P. 1989. Separation of phosphatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography. Journal of Lipid Research 30:761-764. 86. Meyer, V. 2008. Genetic engineering of filamentous fungi—progress, obstacles and future trends. Biotechnology Advances 26:177-185. 87. Meyer, V., Mueller, D., Strowig, T., and Stahl, U. 2003. Comparison of different transformation methods for Aspergillus giganteus. Current Genetics 43:371-377. 88. Michielse, C. B., van Wijk, R., Reijnen, L., Cornelissen, B. J., and Rep, M. 2009. Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f. sp. lycopersici through large-scale insertional mutagenesis. Genome Biology 10:R4. 89. Mimouni, M., Khardli, F., Warad, I., Ahmad, M., Mubarak, M., Sultana, S., and Hadda, T. 2014. Antimicrobial activity of naturally occurring antibiotics Monensin, Lasalocid and their metal complexes. Journal of Materials and Environmental Science 5:207-214. 90. Moffat, J. G., Rudolph, J., and Bailey, D. 2014. Phenotypic screening in cancer drug discovery—past, present and future. Nature reviews Drug Discovery 13:588. 91. Mollenhauer, H. H., Morre, D. J., and Rowe, L. D. 1990. Alteration of intracellular traffic by monensin - mechanism, specificity and relationship to toxicity. Biochimica et Biophysica Acta - Reviews on Biomembranes 1031:225-246. 92. Mostert, D., Molina, A. B., Daniells, J., Fourie, G., Hermanto, C., Chao, C.-P., . . . Thangavelu, R. 2017. The distribution and host range of the banana Fusarium wilt fungus, Fusarium oxysporum f. sp. cubense, in Asia. PLoS One 12:e0181630. 93. Mullins, E., and Kang, S. 2001. Transformation: a tool for studying fungal pathogens of plants. Cellular and Molecular Life Sciences CMLS 58:2043-2052. 94. Muthukumar, K., and Nachiappan, V. 2013. Phosphatidylethanolamine from phosphatidylserine decarboxylase 2 is essential for autophagy under cadmium stress in Saccharomyces cerevisiae. Cell Biochemistry and Biophysics 67:1353-1363. 95. Newman, D. 2017. Screening and identification of novel biologically active natural compounds. F1000Research 6. 96. Nishi, A. 1961. Role of polyphosphate and phospholipid in germinating spores of Aspergillus niger. Journal of bacteriology 81:10. 97. Nucci, M., and Anaissie, E. 2007. Fusarium infections in immunocompromised patients. Clinical Microbiology Reviews 20:695-704. 98. O'Donnell, K., Sutton, D. A., Fothergill, A., McCarthy, D., Rinaldi, M. G., Brandt, M. E., . . . Geiser, D. M. 2008. Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex. Journal of Clinical Microbiology 46:2477-2490. 99. O'Donnell, K., Sutton, D. A., Rinaldi, M. G., Magnon, K. C., Cox, P. A., Revankar, S. G., . . . Robinson, J. S. 2004. Genetic diversity of human pathogenic members of the Fusarium oxysporum complex inferred from multilocus DNA sequence data and amplified fragment length polymorphism analyses: evidence for the recent dispersion of a geographically widespread clonal lineage and nosocomial origin. Journal of Clinical Microbiology 42:5109-5120. 100. O'Donnell, K., Gueidan, C., Sink, S., Johnston, P. R., Crous, P. W., Glenn, A., . . . Sarver, B. A. J. 2009. A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology 46:936-948. 101. Ocamb, C. M., Hamm, P. B., and Johnson, D. A. 2007. Benzimidazole resistance of Fusarium species recovered from potatoes with dry rot from storages located in the Columbia basin of Oregon and Washington. American Journal of Potato Research 84:169-177. 102. Ohara, T., and Tsuge, T. 2004. FoSTUA, encoding a basic helix-loop-helix protein, differentially regulates development of three kinds of asexual spores, macroconidia, microconidia, and chlamydospores, in the fungal plant pathogen Fusarium oxysporum. Eukaryotic cell 3:1412-1422. 103. Ohara, T., Inoue, I., Namiki, F., Kunoh, H., and Tsuge, T. 2004. REN1 is required for development of microconidia and macroconidia, but not of chlamydospores, in the plant pathogenic fungus Fusarium oxysporum. Genetics 166:113-124. 104. Palm, M. E., Gams, W., and Nirenberg, H. I. 1995. Plectosporium, a new genus for Fusarium tabacinum, the anamorph of Plectosphaerella cucumerina. Mycologia:397-406. 105. Palusinska-Szysz, M., Kania, M., Turska-Szewczuk, A., Danikiewicz, W., Russa, R., and Fuchs, B. 2014. Identification of unusual phospholipid fatty acyl compositions of Acanthamoeba castellanii. PloS One 9:e101243. 106. Paphitou, N. I., Ostrosky-Zeichner, L., Paetznick, V. L., Rodriguez, J. R., Chen, E., and Rex, J. H. 2002. In vitro activities of investigational triazoles against Fusarium species: effects of inoculum size and incubation time on broth microdilution susceptibility test results. Antimicrob Agents Chemother 46:3298-3300. 107. Pareek, M., and Rajam, M. V. 2017. RNAi-mediated silencing of MAP kinase signalling genes (Fmk1, Hog1, and Pbs2) in Fusarium oxysporum reduces pathogenesis on tomato plants. Fungal Biology 121:775-784. 108. Pfaller, M. A., Sheehan, D. J., and Rex, J. H. 2004. Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clinical Microbiology Reviews 17:268-280. 109. Pressman, B. C., Harris, E. J., Jagger, W. S., and Johnson, J. H. 1967. Antibiotic-mediated transport of alkali ions across lipid barriers. Proceedings of the National Academy of Sciences 58:1949-1956. 110. Pritsch, K., and Garbaye, J. 2011. Enzyme secretion by ECM fungi and exploitation of mineral nutrients from soil organic matter. Annals of Forest Science 68:25-32. 111. Pujol, I., Guarro, J., Gene, J., and Sala, J. 1997. In-vitro antifungal susceptibility of clinical and environmental Fusarium spp. strains. Journal of Antimicrobial Chemotherapy 39:163-167. 112. Punt, P. J., and van den Hondel, C. A. 1992. Transformation of filamentous fungi based on hygromycin b and phleomycin resistance markers. Pages 447-457 in: Methods in Enzymology, vol. 216. Elsevier. 113. Ramyabharathi, S., Meena, B., and Raguchander, T. 2012. Induction of chitinase and β-1, 3-glucanase PR proteins in tomato through liquid formulated Bacillus subtilis EPCO 16 against Fusarium wilt. Journal of Today’s Biological Sciences-Research & Review 1:50-60. 114. Rasconi, S., Jobard, M., Jouve, L., and Sime-Ngando, T. 2009. Use of calcofluor white for detection, identification, and quantification of phytoplanktonic fungal parasites. Applied and Environmental Microbiology 75:2545-2553. 115. Rasooli, I., and Abyaneh, M. R. 2004. Inhibitory effects of Thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control 15:479-483. 116. Reis, E. M., Boareto, C., Danelli, A. L. D., and Zoldan, S. M. 2016. Anthesis, the infectious process and disease progress curves for Fusarium head blight in wheat. Summa Phytopathologica 42:134-139. 117. Rios, J. L., Recio, M. C., and Villar, A. 1988. Screening methods for natural-products with antimicrobial activity - a review of the literature. Journal of Ethnopharmacology 23:127-149. 118. Robinow, C., and Caten, C. 1969. Mitosis in Aspergillus nidulans. Journal of Cell Science 5:403-431. 119. Rodrigues, A. A. C., and Menezes, M. 2005. Identification and pathogenic characterization of endophytic Fusarium species from cowpea seeds. Mycopathologia 159:79-85. 120. Rodriguez-Iglesias, A., and Schmoll, M. 2015. Protoplast transformation for genome manipulation in fungi. Pages 21-40 in: Genetic Transformation Systems in Fungi, Volume 1. Springer. 121. Roncero, M. I. G., Hera, C., Ruiz-Rubio, M., Garcı́, F. I., Madrid, M. P., Caracuel, Z., . . . Martı́, A. L. 2003. Fusarium as a model for studying virulence in soilborne plant pathogens. Physiological and Molecular Plant Pathology 62:87-98. 122. Rosenberger, R., and Kessel, M. 1967. Synchrony of nuclear replication in individual hyphae of Aspergillus nidulans. Journal of Bacteriology 94:1464-1469. 123. Ruiz-Roldán, M. C., Köhli, M., Roncero, M. I. G., Philippsen, P., Di Pietro, A., and Espeso, E. A. 2010. Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum. Eukaryotic Cell 9:1216-1224. 124. Sabatelli, F., Patel, R., Mann, P. A., Mendrick, C. A., Norris, C. C., Hare, R., . . . McNicholas, P. M. 2006. In vitro activities of posaconazole, fluconazole, itraconazole, voriconazole, and amphotericin B against a large collection of clinically important molds and yeasts. Antimicrobial Agents and Chemotherapy 50:2009-2015. 125. Saini, A., Jalali, I., and Pal, V. 2005. Eco-friendly management of Fusarium wilt root-knot nematode complex in tomato. Journal of Mycology and Plant Pathology 35:338-341. 126. Sant, D. G., Tupe, S. G., Ramana, C. V., and Deshpande, M. V. 2016. Fungal cell membrane - promising drug target for antifungal therapy. Journal of Applied Microbiology. 127. Sarver, B. A., Ward, T. J., Gale, L. R., Broz, K., Kistler, H. C., Aoki, T., . . . O’Donnell, K. 2011. Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance. Fungal Genet Biol. 48:1096-1107. 128. Sawada, K. 1919. Descriptive Catalogue of the Formosan Fungi Part 1. Formosa Agricultural Experiment Station Special Bulletin 19. 129. Schmitz, S., Weidenboerner, M., and Kunz, B. 1993. Herbs and spices as selective inhibitors of mould growth. Chemie, Mikrobiologie, Technologie der Lebensmittel (Germany). 130. Skipski, V., Peterson, R., and Barclay, M. 1964. Quantitative analysis of phospholipids by thin-layer chromatography. Biochemical Journal 90:374. 131. Son, H., Seo, Y.-S., Min, K., Park, A. R., Lee, J., Jin, J.-M., . . . Kim, E.-K. 2011. A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum. PLoS Pathogens 7:e1002310. 132. Song, X.-S., Li, H.-P., Zhang, J.-B., Song, B., Huang, T., Du, X.-M., . . . Agboola, R. S. 2014. Trehalose 6-phosphate phosphatase is required for development, virulence and mycotoxin biosynthesis apart from trehalose biosynthesis in Fusarium graminearum. Fungal Genetics and Biology 63:24-41. 133. Summerell, B. A., Salleh, B., and Leslie, J. F. 2003. A utilitarian approach to Fusarium identification. Plant Disease 87:117-128. 134. Tajima, K., Miyake, T., Koike, N., Hattori, T., Kumakura, S., Yamaguchi, T., . . . Goto, H. 2014. In vivo challenging of polymyxins and levofloxacin eye drop against multidrug-resistant Pseudomonas aeruginosa keratitis. Journal of Infection and Chemotherapy 20:343-349. 135. Truong, T. T., Seyedsayamdost, M., Greenberg, E. P., and Chandler, J. R. 2015. A Burkholderia thailandensis acyl-homoserine lactone-independent LuxR homolog that activates production of the cytotoxin malleilactone. Journal of Bacteriology:JB. 00425-00415. 136. Tsai, J.-N., Cheng, S.-F., Huei-Lin, T., Huang, H.-C., Hsieh, W.-H., and Ann, P.-J. 2013. Use of polycarbonate membrane for detection of extracellular enzymes of Phellinus noxius on solid media. Journal of Taiwan Agricultural Research 62:184-194. 137. Tsai, S.-J. 2012. Development of a gas chromatography-mass spectrometry method for metabolic profiling of fatty acid in plasma. Graduate Institute of Pharmaceutical Sciences College of Medicine, National Taiwan University Master Thesis. 138. Tzeng, D.-S. 2016. Pharmacology and Application of Pesticide─Insecticides (農藥藥理與應用─殺蟲劑). Yi Hsien Publishing Co., Ltd, New Taipei City, Taiwan. (In Chinese). pp. 139. Vance, J. E., and Vance, D. E. 2008. Biochemistry of Lipids, Lipoproteins and Membranes, 5th Edition. Elsevier. pp. 59-244. 140. Voigt, C. A., Schäfer, W., and Salomon, S. 2005. A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals. The Plant Journal 42:364-375. 141. Wang, C.-L., and Cheng, Y.-H. 2017. Identification and trichothecene genotypes of Fusarium graminearum species complex from wheat in Taiwan. Botanical Studies 58:4. 142. Wang, J. H., Ndoye, M., Zhang, J. B., Li, H. P., and Liao, Y. C. 2011. Population structure and genetic diversity of the Fusarium graminearum species complex. Toxins (Basel) 3:1020-1037. 143. Wang, R. H., Wang, S. S., Lin, D. L., Hsieh, M. H., Lin, T. T., Chao, H. F., and Chen, N. C. 2003. Breeding of a new cherry tomato variety “Tainan ASVEG No. 11'. Research Bulletin of Tainan District Agricultural Research and Extension Station 42:23-31. 144. Wang, Z., and Benning, C. 2011. Arabidopsis thaliana polar glycerolipid profiling by thin layer chromatography (TLC) coupled with gas-liquid chromatography (GLC). Journal of Visualized Experiments. 145. Wilson, M. C., Mori, T., Rückert, C., Uria, A. R., Helf, M. J., Takada, K., . . . Schmitt, S. 2014. An environmental bacterial taxon with a large and distinct metabolic repertoire. Nature 506:58. 146. Yang, S.-S., Chen, J.-T., and Chyuan, J.-H. 2008. Breeding of a new cherry tomato variety 'Hualien ASVEG No. 21'. Research Bulletin of Hualien District Agricultural Research and Extension Station 26:65-79. 147. Zhang, C., Lin, Y., Wang, J., Wang, Y., Chen, M., Norvienyeku, J., . . . Wang, Z. 2015. FgNoxR, a regulatory subunit of NADPH oxidases, is required for female fertility and pathogenicity in Fusarium graminearum. FEMS Microbiology Letters 363:fnv223. 148. Zhang, N., O'Donnell, K., Sutton, D. A., Nalim, F. A., Summerbell, R. C., Padhye, A. A., and Geiser, D. M. 2006. Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment. Journal of Clinical Microbiology 44:2186-2190. 149. Zheng, Q., Hou, R., Juanyu, Zhang, Ma, J., Wu, Z., . . . Xu, J. R. 2013. The MAT locus genes play different roles in sexual reproduction and pathogenesis in Fusarium graminearum. PloS One 8:e66980. 150. Zheng, Q., Hou, R., Juanyu, Zhang, Ma, J., Wu, Z., . . . Xu, J. R. 2015. Correction: The MAT locus genes play different roles in sexual reproduction and pathogenesis in Fusarium graminearum. PloS One 10:e0131623. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70252 | - |
dc.description.abstract | 鐮孢菌 (Fusarium spp.)具病原性者影響層面廣泛,又食安問題、環境的永續性近年來備受重視,許多研究者投入非農藥資材的開發,而從巨觀至基因層次詳細瞭解病原性、抗性或藥物對病原的作用機制,將有助於更準確的對症下藥或開發新藥。
本研究共分兩部分,第一部分研究自多種天然資材中,篩選出天然資材孟寧素 (monensin),經過最低抑菌濃度 (minimum inhibitory concentration, MIC)與最低殺菌濃度 (minimum fungicidal concentration, MFC)測試證實,孟寧素對7株來自於植物或人類之病原性鐮孢菌的MIC值最低達16 μg/mL,抑菌效果屬靜菌作用。孟寧素抑制鐮孢菌菌絲生長、影響隔膜生成,對F. oxysporum與F. graminearum之抑制效果較佳,但對F. solani則較差。由亞甲基藍染色與DAPI染色,結果顯示經孟寧素處理後之鐮孢菌細胞仍可存活,但細胞核的分離受到影響。植物病害防治方面,孟寧素較適合作為保護劑於病原入侵植物組織前或於初期感染時施用。若欲使用孟寧素針對人體病原性鐮孢菌進行治療,可以添加於眼藥水中以防範鐮孢菌於其中滋生。 第二部分研究中,對F. graminearum PH-1 (Fg)與F. oxysporum f. sp. lycopersici 4287 (Fol)建立psd2突變株以探討PSD2基因功能。PSD2基因是一磷脂質生合成相關基因,本研究探討此基因對phosphatidylethanolamine (PE)生合成、菌絲生長、產生各種孢子與致病性等能力之影響。在薄層層析試驗中可見Fg與Fol psd2突變株之PE生成量較野生株少。比較添加ethanolamine前後之菌株在微量元素培養基 (minimal medium, MM)之生長情形,發現ethanolamine可恢復Fg與Fol psd2突變株之營養缺陷。Fg與Fol之psd2突變株在MM和PDA培養基生長較野生株緩慢。在產孢方面,PSD2基因影響Fg分生孢子形態與有性世代子囊殼的形態,也影響Fol之分生孢子發芽率、厚膜孢子之產孢與其在菌絲上的著生形式。PSD2基因影響Fg胞外酵素分泌,但不影響Fg與Fol對小麥或番茄寄主之毒力。 | zh_TW |
dc.description.abstract | Pathogenic Fusarium species have a broad impact worldwide. Due to the rising attention on food safety and environmental sustainability issues, we thus interested to develop non-pesticide materials. In discovering control methods through obtaining a better knowledgement of pathogenicity, drug resistance, or mechanism of drugs on pathogens from giant to gene level, we would be able to solve problem accurately or discover new drug effectively.
There are two parts in this study. In the first part, monensin, a nature compound, was discovered and tested its minimum inhibitory concentration (MIC) equal or larger to 16 μg/mL against seven plant or human pathogenic Fusarium species. Inhibitory effect of monensin on Fusarium species is fungistatic. Monensin affected hyphal growth, formation of septa, and nuclear division of Fusarium species. Compared with F. solani, monensin had stronger inhibition ability against F. oxysporum, especially F. oxysporum f. sp. lycopersici 4287 (Fol) in peat extract, and F. graminearum PH-1 (Fg). For controlling Fusarium infections on plants, monensin can be recommended as protectant, and for controlling human Fusarium infections, it might be added in eye drops or contact lens solution to prevent keratitis. In the second part of study, Δpsd2 mutants of Fg and Fol were constructed and the influence of PSD2 gene, encoding phosphatidylserine decarboxylase, on phosphatidylethanolamine (a kind of phospholipid) production, hyphal growth, production of conidia or chlamydospores, and virulence in the plant hosts was tested. Comparing the growth of strains in the absence or presence of ethanolamine (Etn) in minimal medium (MM), Δpsd2 mutants of both Fg and Fol exhibited Etn auxotrophy. Δpsd2 mutants of both Fg and Fol grew slower on MM and PDA, while similar on barley bran media compared with their wild type, respectively. PSD2 gene affected conidiation and perithecia morphology of Fg. It also affected the germination rate of conidia, production and formation of chlamydospores of Fol. Although PSD2 gene affected secretion of extracellular enzymes of Fg, it is dispensable to virulence of Fg and Fol on wheat or tomato hosts. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:24:49Z (GMT). No. of bitstreams: 1 ntu-107-R04633002-1.pdf: 5896472 bytes, checksum: 84c069cb238e89dee6586217f2410f1d (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 目 錄
口試委員會審定書 I 致謝 II 中文摘要 IV 英文摘要 V 內文目錄 ⅤI 內文目錄 壹、 引言 1 1. 鐮孢菌之重要性 1 2. 綜合防治方法探討─藥物開發與基因研究 2 3. 研究目的 4 貳、 第一部分─孟寧素具有抑制鐮孢菌之能力 5 1. 孟寧素簡介 5 2. 材料與方法 6 2.1. Fg培養條件之優化 6 2.2. 菌株來源、培養與保存 6 2.3. 孢子懸浮液製備 6 2.4. 對番茄萎凋病菌具抑制效果之天然資材篩選 7 2.5. 濾紙片擴散試驗 7 2.6. 最低抑菌濃度測試 7 2.7. 最低殺菌濃度測試 8 2.8. 孟寧素對菌絲生長與隔膜形成之影響 8 2.9. 孟寧素對產孢梗形成之影響 9 2.10. 孟寧素對細胞核產生之影響 10 2.11. 孟寧素對孢子發芽率之影響 10 2.12. 孟寧素對鐮孢菌於泥炭土萃取液中生長與厚膜孢子產量之影響 11 3. 結果 12 3.1. 孟寧素對F. oxysporum f. sp. lycopersici具有抑制能力 12 3.2. 孟寧素對F. oxysporum與F. graminearum具有較強的抑制能力 12 3.3. 孟寧素之抑制效果屬於靜菌作用 12 3.4. 孟寧素可抑制菌絲生長、隔膜生成 13 3.5. 孟寧素不抑制產孢梗生成 13 3.6. 孟寧素處理後之F. oxysporum菌絲中央細胞產生多核 14 3.7. 孟寧素抑制植物病原鐮孢菌之孢子發芽率 14 3.8. 孟寧素抑制F. oxysporum f. sp. lycopersici於模擬土壤之環境中生長而不影響厚膜孢子產量 14 4. 討論 16 參、 第二部分─PSD2基因影響小麥赤黴病菌與番茄萎凋病菌脂質組成、生長與產孢 20 1. 前言 20 1.1. 鐮孢菌之病害生態 20 1.2. PSD2基因簡介 21 2. 材料與方法 23 2.1. 菌株來源、培養與儲存 23 2.2. 孢子懸浮液製備 23 2.3. psd2突變株建立 23 2.3.1. 比對Fg與Fol之PSD2之基因序列 23 2.3.2. 含抗藥性基因之質體純化 23 2.3.3. 真菌之DNA純化 24 2.3.4. 原生質體 (protoplast)製備 25 2.3.5. Fusion PCR 25 2.3.6. 以PEG菌株轉型法製作突變株 26 2.3.7. 以PCR方式確認psd2突變株 27 2.4. PSD2基因功能研究 28 2.4.1. 磷脂質萃取與成分分析 28 2.4.2. 以Kennedy pathway代替de novo pathway合成PE之能力測試 29 2.4.3. 生長速率測試與菌落形態比較 29 2.4.4. PSD2基因對分生孢子形態之影響 29 2.4.5. PSD2基因對分生孢子發芽率之影響 30 2.4.6. PSD2基因對Fol厚膜孢子之影響 30 2.4.7. Fg有性生殖測試 30 2.4.8. 胞外酵素活性測試 31 2.4.9. 病原性測試 32 3. 結果 33 3.1. psd2突變株確認轉型成功 33 3.2. PSD2基因缺陷導致Fg與Fol之PE生成量減少 33 3.3. 外源ethanolamine可使psd2突變株恢復於MM上之生長能力 33 3.4. PSD2基因影響菌株於特定培養基上之生長能力 33 3.5. PSD2基因影響Fg之部分分生孢子長度 34 3.6. PSD2基因影響Fol之分生孢子發芽率 34 3.7. PSD2基因影響Fol之厚膜孢子著生形式與產量 35 3.8. PSD2基因影響Fg子囊殼之形態 35 3.9. PSD2基因影響Fg澱粉與脂質分解酵素之分泌 35 3.10. PSD2基因不影響Fg與Fol於寄主之毒力 35 4. 討論 37 肆、 參考文獻 43 伍、 附錄 92 1. 含zeocin (phleomycin)抗藥性基因之質體純化 92 2. 以fusion PCR方式建立Fg之PSD2互補株 92 3. 以fusion PCR方式建Fg之cho1、psd1突變株 93 4. 以fusion PCR輔以split marker方式建立PSD2互補株 93 5. 以fusion PCR輔以split marker方式建立cho1、psd1突變株 94 6. Fg、Fol之PSD2互補株、cho1與psd1突變株建立結果與討論 96 7. (補充表五)Selleck Chemicals公司出產之天然資材庫 107 8. 口試委員意見紀錄 117 表目錄 表一、第一部分研究之菌株 57 表二、孟寧素對鐮孢菌菌株之最低抑菌濃度與最低殺菌濃度 58 表三、第二部分研究使用之菌株 59 表四、第二部分研究使用之引子 60 補充表一、建立Fusarium graminearum與F. oxysporum f. sp. lycopersici之cho1、psd1突變株與PSD2補充株所使用之引子 97 補充表二、建立Fusarium graminearum之cho1、psd1突變株與PSD2補充株所使用之PCR條件 99 補充表三、建立Fusarium oxysporum f. sp. lycopersici之cho1、psd1突變株與PSD2補充株所使用之PCR條件 101 補充表四、Fusarium graminearum與F. oxysporum f. sp. lycopersici PSD2互補株與cho1、psd1突變株所使用之PCR條件 102 補充表五、Selleck Chemicals公司出產之天然資材庫 107 圖目錄 圖一、孟寧素對鐮孢菌具有抑制效果 62 圖二、孟寧素之抑菌效果為靜菌作用 63 圖三、孟寧素處理後之菌絲生長速率緩慢且形態腫脹 66 圖四、孟寧素不抑制產孢梗生成 67 圖五、孟寧素處理後之F. oxysporum菌絲中央細胞產生多核 69 圖六、孟寧素抑制植物病原鐮孢菌之孢子發芽率 71 圖七、孟寧素減少F. oxysporum f. sp. lycopersici在泥炭土萃取液中之菌絲乾重 72 圖八、孟寧素不影響F. oxysporum厚膜孢子之產生 73 圖九、真菌之磷脂質代謝路徑。酵母菌可利用兩種磷脂質代謝路徑Kennedy pathway與de novo pathway 74 圖十、psd2突變株轉型與確認 76 圖十一、psd2基因缺陷導致Fg與Fol之PE生成量減少 78 圖十二、Ethanolamine (Etn)可恢復Fg與Fol之psd2突變株之營養缺陷 79 圖十三、psd2突變株於不同培養基上之生長速率不同 80 圖十四、PSD2基因影響Fg之部分分生孢子長度 82 圖十五、PSD2基因影響Fol之分生孢子發芽率 84 圖十六、PSD2基因影響Fol之厚膜孢子產生方式與產量 85 圖十七、PSD2基因影響Fg子囊殼之形態 86 圖十八、PSD2基因影響Fg澱粉與脂質分解酵素之分泌 87 圖十九、PSD2基因不影響Fg之毒力 89 圖二十、PSD2基因不影響Fol之毒力 90 補充圖一、Fg以fusion PCR與PEG轉型法製作PSD2互補株與cho1、psd1突變株 103 補充圖二、Fg與Fol以split marker的方式進行fusion PCR以製作PSD2互補株 104 補充圖三、Fg與Fol以split marker的方式進行fusion PCR以製作cho1、psd1突變株 105 | |
dc.language.iso | zh-TW | |
dc.title | 從藥物開發與基因研究探討鐮孢菌之防治方法 | zh_TW |
dc.title | Investigating Control Methods against Fusarium Species from Novel Drug Discovery and Gene Study | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾文鑫,王智立,林乃君,鍾嘉綾 | |
dc.subject.keyword | 鐮孢菌,孟寧素,天然資材,小麥赤黴病,番茄萎凋病,磷脂質, | zh_TW |
dc.subject.keyword | Fusarium,monensin,natural product,Fusarium head blight,tomato Fusarium wilt,PSD2,phospholipid, | en |
dc.relation.page | 125 | |
dc.identifier.doi | 10.6342/NTU201803507 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-15 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 5.76 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。