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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 胡文聰(Andrew M. Wo) | |
| dc.contributor.author | Pei-Chen Chuang | en |
| dc.contributor.author | 莊沛晟 | zh_TW |
| dc.date.accessioned | 2021-05-17T09:23:55Z | - |
| dc.date.available | 2012-12-10 | |
| dc.date.available | 2021-05-17T09:23:55Z | - |
| dc.date.copyright | 2012-12-10 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-20 | |
| dc.identifier.citation | (1) P. S. Steeg Nat Med 2006, 12 (8), 895-904.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7011 | - |
| dc.description.abstract | 循環癌細胞 (circulating tumor cells, CTCs) 檢測是一可期待且對病人較少傷害癌症轉移檢測方法。臨床中癌症造成的死亡有九成是來自於癌細胞的轉移，其轉移路徑通常經由進入血管後轉移至腦、骨髓、肺臟、肝臟或其他器官。然而目前循環癌細胞檢測仍未被常規性使用於疾病管理，其中一個原因來自於稀少細胞的汲取困難。本工作及透過一內含以尺寸為篩選標準的微過濾結構之微流晶片從血液中汲取循環癌細胞，其製造方法為精密塑膠射出成形的壓克力製微結構熱壓接合到以電腦數值控制铣床製的壓克力儲存槽。廣範圍之癌症細胞株 (K562, MCF7, PC3 與 Colo205) 被微結構抓取後以螢光染色並以螢光顯微技術計數。結果顯示此晶片之穩定與易用性，以上述細胞株加入一毫升血液 (稀釋為十五毫升) 模擬病人檢體中的循環癌細胞抓取，其回收率達到七至八成。故此微流晶片應擁有讓循環癌細胞檢測成為更可用之癌症管理工具之潛力。 | zh_TW |
| dc.description.abstract | The detection of circulating tumor cells (CTCs) is a promising harmless in vitro diagnosis of metastasis. Cancer metastasis leads to roughly 90% of deaths of cancer patients. However, CTC detection has not been routinely used for disease management, where one reason for this is the difficulty associated with enrichment of these extremely rare cells. This work presents a microdevice to enrich CTCs from peripheral blood by a size-limiting microfilter structure. This PMMA structure is microinjection-mold and bonded onto a CNC-milled reservoir. Cells from a wide range of cancer cell lines (K562, MCF7, PC3 and Colo205) are captured on the structure, immunohistochemically stained and enumerated with fluorescent microscopy. Results show the chip is robust and ease to use. The recovery yield of cells spiked into 1mL of whole blood (diluted to 15mL) attained 70% to 80%. This microdevice should have potential to enable CTC-detection a more viable in cancer management. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-17T09:23:55Z (GMT). No. of bitstreams: 1 ntu-101-R99543095-1.pdf: 1593681 bytes, checksum: 81a80d023ffdc88795c047e232206b86 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | CONTENTS
口試委員會審定書 # 誌謝 i 中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES vii Chapter 1 Introduction 1 Chapter 2 Experimental 4 2.1 Device design 4 2.2 Device Fabrication 6 2.3 Device assembly 7 2.4 Experimental setup 9 2.5 Experimental procedure 10 2.6 Cell culture and preparation 10 2.7 Cell size measurement 12 2.8 Blood dilution and preparation 12 2.9 Cell viability assay and culture on chip 13 2.10 Immunofluorescent (IF) analysis 13 Chapter 3 Results and Discussion 14 3.1 Capture efficiency and cell size 14 3.2 Effects of ΔP and cell condition on yield 17 3.3 Viability studies of captured cells 18 3.4 Effects of dilution ratio 19 3.5 Yield of Tumor cell lines spiked in healthy blood 21 Chapter 4 Conclusion 22 Reference 23 Appendix A Design concepts of apertures on the micro-filter 27 Appendix B Thermal bonding chamber system 33 | |
| dc.language.iso | en | |
| dc.title | 應用可量產之微流體篩選晶片分離人類血液中的轉移性癌細胞 | zh_TW |
| dc.title | A Mass-producible Filtration Chip for Isolation of Circulating Tumor Cells from Human Blood | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江宏仁,林璟宏 | |
| dc.subject.keyword | 尺寸,稀少細胞,細胞分離,細胞抓取,低價,壓克力,射出成形,量產,循環癌細胞,癌症檢測, | zh_TW |
| dc.subject.keyword | size-based,rare cell,cell separation,cell isolation,low cost,PMMA,injection molding,mass-production,Circulating tumor cells,CTCs, | en |
| dc.relation.page | 33 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2012-08-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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