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標題: | 阿拉伯芥NRT1/PTR Family 2.11 (NPF2.11/GTR2)參與在非生物逆境的生理調控 NRT1/PTR Family 2.11 (NPF2.11/GTR2) is involved in abiotic stress responses in Arabidopsis |
作者: | Kim Teng Lee 李金騰 |
指導教授: | 王雅筠(Ya-Yun Wang) |
關鍵字: | 阿拉伯芥,硝酸根離子,NPF2.11/GTR2,非生物逆境,硝酸根離子分佈, Arabidopsis,nitrate,NPF2.11/GTR2,abiotic stress,nitrate allocation, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 氮元素是所有生命體必要的元素,大部分的陸地植物以吸收硝酸根離子為氮的來源。我們從網路公開平台上的微陣列數據對一群具有運輸硝酸根離子能力的阿拉伯芥轉運蛋白家族進行基因表現量的分析,從53個成員中挑選出一些有可能參與在非生物逆境的候選基因。針對NPF2.11/GTR2基因進行即時聚合酶連鎖反應(qRT-PCR)分析其在鹽逆境下的基因表現含量,結果顯示鹽逆境在十二小時內有誘導其基因表現的情況。此外gtr2-1突變株對鹽逆境下所誘導的主根長抑制現象比野生型(Col-0)較為不敏感。與此同時,gtr2突變株相較於野生型有更低的水分散失率以及氣孔導度。在氯化鉀(KCl)處理下gtr2突變株的氣孔孔徑較野生型來得小,而這一個現象可以經由處理硝酸根離子恢復到與野生型相同的孔徑。然而在正常條件下gtr2-1在地上部的硝酸根離子含量較野生型高,在鹽逆境下gtr2突變株中硝酸根離子在地上部與根部的比值較野生型高。此外鹽逆境在gtr2突變株中所誘導的 NPF7.3/NRT1.5 及NPF7.2/NRT1.8與野生型有相似的樣式。總結以上的試驗結果,可以推測出GTR2參與在鹽逆境以及乾旱逆境下的生理調控,並且與鹽逆境下所誘導的根部累積硝酸根離子現象(SINAR)不相符合。 Nitrogen is an essential element of living organisms. Most of the land plants use nitrate (NO3-) as the major nitrogen source. Nitrate transporter 1/peptide transporter family (NPF) is one of the transporter families, which is able to transport nitrate in plants. We analyzed the online public micro-array database of the 53 members of NPF and found out the candidate members that may participate in the responses upon abiotic stresses. The real-time quantitative RT-PCR results showed that NPF2.11/GTR2 is upregulated by salt stress within 12 hours, and showed a less sensitivity of salt-induced inhibition on primary root growth in the gtr2-1 mutant. In addition, gtr2 mutants had lower water loss rate of leaves and lower stomatal conductance than Col-0. The stomatal aperture results showed that gtr2 mutants had functional deficient in stomata under KCl treatment, and this functional deficient can be recovered by supplement nitrate. However, there was more nitrate allocated to shoot in gtr2-1 mutant under normal condition and higher shoot-to-root nitrate content ratio in gtr2 mutants under salt stress. Furthermore, the expression level of NPF7.3/NRT1.5 and NPF7.2/NRT1.8 had similar salt-induced pattern between gtr2 mutants and Col-0. Taken together, this study suggests that GTR2 involved in salt and drought stresses and might not correlate to stress-initiated nitrate allocation to roots (SINAR) in Arabidopsis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69846 |
DOI: | 10.6342/NTU201800631 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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