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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫啟光 | |
dc.contributor.author | Kuan-Hung Lin | en |
dc.contributor.author | 林冠宏 | zh_TW |
dc.date.accessioned | 2021-06-17T03:28:32Z | - |
dc.date.available | 2020-04-18 | |
dc.date.copyright | 2018-04-18 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-03-15 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69801 | - |
dc.description.abstract | 內在皮膚老化是一個皮膚表皮層與真皮層構造與年齡相關變化的不可逆過程。對皮膚醫學和化妝品工業來說,高加索人與亞洲人間的皮膚顯型與作用上的內在老化差異一直是一個有趣的議題。有許多文獻描述種族間的皮膚構造特性差異。然而,大多數的文獻都將分析重點放在角質層,而有些文獻發現某些皮膚特性的個體年齡間差異勝過了種族間的差異。因此,描述更多的皮膚參數與不同種族間的老化差異是近來的需求。本研究調查高加索人與亞洲人間在活體上皮層與真皮乳突區域這些與皮膚內在老化相關的形態改變。與其他顯微術相比,倍頻顯微術是一個最適合研究皮膚老化的工具,因為倍頻效應有分辨表皮層與真皮層的能力。我們將一台波長為1230奈米的飛秒鉻:貴橄欖石雷射作為光源以降低皮膚光衰減。與顯微鏡和雷射掃描系統結合,可以分別獲得次微米和一微米的橫向和縱向解析度和大於300微米的穿透深度。在本研究,我們徵求了31個高加索人受試者以獲取前手臂內側不受光照的活體倍頻顯微影像。結合我們之前亞洲人的分析研究,讓我們得以比較分析高加索人與亞洲人間的內在皮膚老化差異。
在高加索人與亞洲人的比較分析中,高加索人在活性表皮厚度、顆粒層細胞與細胞核大小和細胞核質比、真皮乳突區域之厚度、單位面積所含真皮乳突體積、真皮乳突區域內真皮乳突體積比例、單位面積所含膠原纖維體積和三維叉合指標都有平均而言較大的尺度。高加索人的基底細胞和細胞核大小隨著年齡增加和亞洲人一樣而有變大的趨勢,而高加索人的最大活性表皮厚度、真皮乳突區域之厚度和單位面積所含真皮乳突體積隨著年齡下降與亞洲人有著顯著不同的趨勢。我們認為主要影響高加索人與亞洲人之間外觀上老化的差異是真皮乳突區域相關的參數,而由於基底細胞和細胞核大小在不同種族間的一致性,可以用做評估皮膚內在老化的評分指標。 | zh_TW |
dc.description.abstract | Intrinsic skin aging is defined as an inalterable process that is associated with the cellular and sub-cellular structural change of epidermis and dermis. Phenotypical and functional differences in the intrinsic skin aging process of individuals between Caucasians and Asians have generated considerable interest in dermatology and cosmetic industry. Some recent works describing racial differences in structural skin aging properties have been reported. However, most of these studies have been focused on stratum corneum and in some other studies interindividual differences in skin quality overwhelm the racial difference. Therefore, there has been a demand for more detailed studies to address various cellular and sub-cellular epidermal/dermal parameters together with age differences among different racial groups. This study investigates the morphological changes related to intrinsic skin aging in the viable epidermis and the dermal papilla zone (DPZ) between Caucasians and Asians. In contrast to other microscopies, harmonic generation microscopy (HGM) is an optimal tool to study skin aging because of the ability to distinguish the epidermis from dermis by different harmonic generation phenomena. A 1230 nm femtosecond chromium-forsterite laser was used for excitation to lessen skin attenuation and a sub-micron and 1-micron resolution in lateral and axial directions and a greater than 300 μm penetrability were previously achieved. In this study, we recruited 31 Caucasian subjects and obtained in vivo HGM images on the sun-protected volar forearm. In combination with our previous results on Asian skin, these studies allow us to comparatively analyze the difference of intrinsic aging between Caucasian and Asian skin.
In comparative analysis between Caucasians and Asians, Caucasian subjects have on average larger scale in the viable epidermis thickness, the cellular size, nuclear size and NC ratio of granular cells, the depth of DPZ, the DP volume per unit area, the collagen volume per unit area, the DP volume ratio and the 3D interdigitation index. The cellular and nuclear sizes of basal cells in Caucasians increase with age with the same trend as in Asians, and the maximum viable epidermis thickness, the depth of DPZ and the DP volume per unit area in Caucasians decrease with age with highly significant difference respect to Asians. Our present findings suggest that the primary factor to result in different aging outlook between Caucasians and Asians is the DPZ-related parameters while the cellular, nuclear size of basal cells can serve as scoring indices for intrinsic skin aging due to their consistency between Caucasians and Asians. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T03:28:32Z (GMT). No. of bitstreams: 1 ntu-107-R04945030-1.pdf: 4362863 bytes, checksum: a7845828d956753eac04ef5314be0e32 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | Abstract i
摘要 iii I. Contents v Figure Contents vii Table Contents xii Chapter 1 Introduction 13 1.1 Skin Aging 13 1.2 Structural changes of the skin 14 1.3 Aging difference between Caucasians and Asians 15 1.4 Harmonic Generation Microscopy 16 1.5 Surveys on Image Segmentation 18 1.5.1 Image thresholding 18 1.5.2 Otsu’s method 19 Chapter 2 Materials and Methods 21 2.1 Study Population 21 2.2 Harmonic Generation Microscope System 21 2.3 Imaging Procedure 23 2.4 Analysis Protocols 26 2.4.1 In vivo Analysis of Viable Epidermis 26 2.4.1.1 Viable Epidermis Thickness 26 2.4.1.2 Cell Size and NC Ratio of Granular Cells 29 2.4.1.3 Cell Size and NC Ratio of Basal Cells 31 2.4.2 In vivo Analysis of Dermal Papilla Zone 32 2.4.2.1 Dermal Papilla Zone 32 2.4.2.2 Depth of Dermal Papilla Zone 33 2.4.2.3 Digital Image Processing for Volume per Unit Area, Volume Ratio, and 3D Interdigitation Index 33 2.4.2.4 2D collagen density in DP and Collagen volume in DP per unit area 37 2.5 Statistical Protocol 39 Chapter 3 Results 42 3.1 In Vivo Image Analysis of Human Skin 42 3.2 In Vivo Analysis of Viable Epidermis for Caucasians 42 3.2.1 Viable Epidermis Thickness 42 3.2.2 Average Area of Granular Cells 44 3.2.3 Average Area of Basal Cells 47 3.3 In Vivo Analysis of Dermal Papillae within Dermal Papilla Zone for Caucasians 49 3.3.1 Depth of Dermal Papilla Zone 49 3.3.2 Dermal Papillae Volume per Unit Area 50 3.3.3 Dermal Papillae Volume Ratio within Dermal Papilla Zone 51 3.3.4 3D Interdigitation Index 53 3.3.5 2D Collagen Density in DP 54 3.3.6 Collagen Volume in DP per Unit Area 54 3.4 Comparative Analysis of Viable Epidermis between Caucasian and Asian 56 3.4.1 Viable Epidermis Thickness 56 3.4.2 Average Area of Granular Cells 59 3.4.3 Average Area of Basal Cells 63 3.5 Comparative Analysis of Dermal Papillae within Dermal Papilla Zone between Caucasian and Asian 67 3.5.1 Depth of Dermal Papilla Zone 67 3.5.2 Dermal Papillae Volume per Unit Area 68 3.5.3 Dermal Papillae Volume Ratio within Dermal Papilla Zone 70 3.5.4 3D Interdigitation Index 71 3.5.5 2D Collagen Density in DP 73 3.5.6 Collagen Volume in DP per Unit Area 74 Chapter 4 Discussion 77 4.1 Overall Results of Aging Analysis between the Caucasian and Asian Subjects 77 4.2 Comparisons with Previous Studies 78 4.2.1 Comparison between Results of Viable Epidermis Thickness 79 4.2.2 Comparison between Results of Average Area of Granular Cells 80 4.2.3 Comparison between Results of Average Area of Basal Cells 81 4.2.4 Comparison between Results of Depth of Dermal Papilla Zone 82 4.2.5 Comparison between Results of 3D Interdigitation Index 83 4.2.6 Comparison between Results of 2D Collagen Density in DP and Collagen Volume per Unit Area 83 4.3 Intrinsic Age-Related Changes in the Skin of Caucasian Subjects 84 4.3.1 Age-Related Changes in the Epidermis 84 4.3.2 Deduction of Age-Related Changes in Dermal Papillae within the Dermal Papilla Zone 86 Chapter 5 Conclusion 91 Appendix 94 References 103 | |
dc.language.iso | en | |
dc.title | 使用活體倍頻顯微術比較分析高加索人與亞洲人間的皮膚內在老化 | zh_TW |
dc.title | Comparative analysis of intrinsic skin aging between Caucasian and Asian subjects by in vivo harmonic generation microscopy | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖怡華,張瑞峰 | |
dc.subject.keyword | 倍頻顯微術,活體成像,內在老化,活性表皮,真皮乳突,高加索人,亞洲人,種族, | zh_TW |
dc.subject.keyword | harmonic generation microscopy,in vivo imaging,intrinsic aging,viable epidermis,dermal papillae,Caucasian,Asian,race, | en |
dc.relation.page | 105 | |
dc.identifier.doi | 10.6342/NTU201800572 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-03-15 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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