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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王兆麟(Jaw-Lin Wang) | |
| dc.contributor.author | Mu-Cyun Tseng | en |
| dc.contributor.author | 曾牧群 | zh_TW |
| dc.date.accessioned | 2021-06-17T03:27:14Z | - |
| dc.date.available | 2021-02-20 | |
| dc.date.copyright | 2021-02-20 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-02-17 | |
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Duncan, The notochordal cell in the nucleus pulposus: a review in the context of tissue engineering. Tissue engineering, 2003. 9(4): p. 667-677. 20. Warden, S.J., A New Direction for Ultrasound Therapy in Sports Medicine. Sports Medicine, 2003. 33(2): p. 95-107. 21. Khanna, A., et al., The effects of LIPUS on soft-tissue healing: a review of literature. British medical bulletin, 2009. 89(1): p. 169-182. 22. Welgus, H.G., J.J. Jeffrey, and A.Z. Eisen, Human skin fibroblast collagenase. Assessment of activation energy and deuterium isotope effect with collagenous substrates. Journal of Biological Chemistry, 1981. 256(18): p. 9516-9521. 23. Miyamoto, K., et al., Exposure to Pulsed Low Intensity Ultrasound Stimulates Extracellular Matrix Metabolism of Bovine Intervertebral Disc Cells Cultured in Alginate Beads. Spine, 2005. 30(21): p. 2398-2405. 24. Hiyama, A., et al., Synergistic effect of low-intensity pulsed ultrasound on growth factor stimulation of nucleus pulposus cells. 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Maroudas, Swelling of the intervertebral disc in vitro. Connective tissue research, 1981. 9(1): p. 1-10. 31. Ariga, K., et al., Mechanical stress-induced apoptosis of endplate chondrocytes in organ-cultured mouse intervertebral discs: an ex vivo study. Spine, 2003. 28(14): p. 1528-1533. 32. Illien-Jünger, S., et al., The combined effects of limited nutrition and high-frequency loading on intervertebral discs with endplates. Spine, 2010. 35(19): p. 1744-1752. 33. Korecki, C.L., et al., Intervertebral disc cell response to dynamic compression is age and frequency dependent. Journal of orthopaedic research, 2009. 27(6): p. 800-806. 34. Urban, J., et al., Nutrition of the intervertebral disc: effect of fluid flow on solute transport. Clinical orthopaedics and related research, 1982(170): p. 296-302. 35. Sivan, S., et al., Diurnal fluid expression and activity of intervertebral disc cells. Biorheology, 2006. 43(3, 4): p. 283-291. 36. Agell, N., et al., Modulation of the Ras/Raf/MEK/ERK pathway by Ca2+, and calmodulin. Cellular signalling, 2002. 14(8): p. 649-654. 37. Cullen, P.J. and P.J. Lockyer, Integration of calcium and Ras signalling. Nature reviews Molecular cell biology, 2002. 3(5): p. 339-348. 38. Chen, H.-J., et al., A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II. Neuron, 1998. 20(5): p. 895-904. 39. Liu, Q., et al., CAPRI and RASAL impose different modes of information processing on Ras due to contrasting temporal filtering of Ca2+. The Journal of cell biology, 2005. 170(2): p. 183-190. 40. Berridge, M.J., Calcium signalling remodelling and disease. 2012, Portland Press Ltd. 41. Kolch, W.J.B.J., Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions. 2000. 351(2): p. 289-305. 42. Cuesta, A., et al., Acid-sensing ion channels in healthy and degenerated human intervertebral disc. Connective tissue research, 2014. 55(3): p. 197-204. 43. Wemmie, J.A., R.J. Taugher, and C.J. Kreple, Acid-sensing ion channels in pain and disease. Nature Reviews Neuroscience, 2013. 14(7): p. 461-471. 44. Uchiyama, Y., et al., Expression of acid‐sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling. Journal of Bone and Mineral Research, 2007. 22(12): p. 1996-2006. 45. Sun, Y., et al., Signaling pathway of MAPK/ERK in cell proliferation, differentiation, migration, senescence and apoptosis. Journal of Receptors and Signal Transduction, 2015. 35(6): p. 600-604. 46. Iwashina, T., et al., Low-intensity pulsed ultrasound stimulates cell proliferation and proteoglycan production in rabbit intervertebral disc cells cultured in alginate. Biomaterials, 2006. 27(3): p. 354-361. 47. Erdogmus, S., et al., Helix 8 is the essential structural motif of mechanosensitive GPCRs. Nature communications, 2019. 10(1): p. 1-15. 48. Berridge, M.J., Calcium signalling and cell proliferation. Bioessays, 1995. 17(6): p. 491-500. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69771 | - |
| dc.description.abstract | 非典型下背痛與椎間盤退化有極大關係，並與其內髓核細胞退化有關，透過探討髓核細胞核內p-ERK訊號以及鈣離子濃度調控來了解髓核細胞受外力刺激時的反應。發展出能夠即時觀察之超音波刺激壓力載台，能在壓力環境下給予超音波刺激，發現NP細胞為一對外力敏感的細胞，在壓力及超音波刺激下p-ERK訊號皆增強，且會增加Sox9表現量，而長時間的壓力刺激也會增加髓核細胞的第二型膠原蛋白製造。以超音波玻璃針尖刺激及快速壓力變化探討髓核細胞的鈣離子反應，超音波刺激會透過ASIC3及GPCR增加細胞內鈣離子濃度，而在動態壓力下，增壓及洩壓時NP細胞也都會有鈣離子的正調控。NP細胞在人體內因從事運動而受到脊椎變形產生椎間盤內壓變化，會透過鈣離子調控進而影響下游細胞訊號，然而無法促進NP細胞增生，但能夠維持其生理功能，分泌細胞外基質，使椎間盤維持於健康狀態。椎間盤退化患者若無法從事運動，超音波治療可能扮演著提供相同刺激之工具，透過不同鈣離子及細胞因子調控路徑，促進NP生理功能，可能減緩椎間盤退化情形，維持椎間盤健康條件。 | zh_TW |
| dc.description.abstract | Ultrasound therapy were used as treatment to atypical low back pain and have been reported effective of patients’ chef complaint, while the mechanism still remains unknown. We investigate the nucleus pulposus cells’ intracellular calcium response and phosphorylation of ERK(p-ERK) to external mechanical force stimulation by hydrostatic pressure and ultrasound-micropipette. NP cells p-ERK was increased and highly translocated into nucleus after pressurized and ultrasonic stimuli. With ultrasound-micropipette investigation, which offering acoustic pressure and acoustic streaming stimuli, intracellular calcium was upregulated through ASIC3 and GPCR. Also, pressure change elevated calcium concentration, whether pressurizing or relief. Hence, we consider exercise could maintain intervertebral disc health with p-ERK activated through calcium signal pathway. Moreover, ultrasound therapy would utilize the high mechanosensitivity of NP to provide similar effect of exercises to NP to retards degeneration of intervertebral disc potentially | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T03:27:14Z (GMT). No. of bitstreams: 1 U0001-1602202100233100.pdf: 4959213 bytes, checksum: 8c8e2da00a8e2f77b9f68787bd26edc1 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 中文摘要 V ABSTRACT VI 第一章、緒論 1 1.1下背痛(LOW BACK PAIN, LBP) 1 1.2椎間盤(INTERVERTEBRAL DISCS, IVD) 2 1.3椎間盤退化(DEGENERATIVE DISC DISEASE, DDD) 3 1.4髓核細胞(NUCLEUS PULPOSUS CELLS, NP CELLS) 4 1.5醫療超音波 5 1.6低能量超音波在髓核細胞(LOW-INTENSITY PULSE ULTRASOUND, LIPUS) 6 1.7靜水壓(HYDROSTATIC PRESSURE) 7 1.8鈣離子-細胞外調節蛋白酶訊號路徑(CALCIUM- EXTRACELLULAR REGULATED PROTEIN KINASES PATHWAY) 8 1.9酸敏感離子通道(ACIDS-SENSING IONS CHANNEL, ASICS) 10 第二章、壓力下超音波刺激對髓核細胞之調控 12 2.1髓核細胞(NUCLEUS PULPOSUS)準備 12 2.1.1髓核細胞初代培養(Primary Culture) 12 2.1.2細胞繼代(Cell Culture) 13 2.2壓力作用環境之超音波刺激設備校正 14 2.2.1壓力刺激影像載台(Pressurized Imaging Chamber, PIC) 14 2.2.2壓力刺激影像載台之超音波壓台設計 15 2.2.3壓力刺激氣壓液壓轉換裝置設計 16 2.2.4壓力刺激影像載台實驗架設 17 2.2.5壓力刺激影像載台超音波校正實驗架設 17 2.2.6壓力刺激影像載台溫度校正 19 2.2.7壓力刺激設備設計 21 2.2.8壓力刺激腔體實驗架設 21 2.3髓核細胞靜水壓與超音波刺激P-ERK訊號實驗 22 2.3.1實驗設計 22 2.3.2免疫螢光染色(Immunoflourescent)細胞準備 23 2.3.3西方墨點法(Western Blot)細胞準備 24 2.3.4壓力環境及超音波刺激實驗結果 25 2.3.5壓力刺激模式對p-ERK影響實驗 30 2.3.6壓力刺激對髓核細胞的增生實驗 32 2.4髓核細胞靜水壓與超音波刺激相關因子實驗 34 2.4.1訊息核糖核酸(messengerRNA)檢測細胞準備 34 2.4.2髓核細胞靜水壓與超音波刺激mRNA實驗 35 2.4.3西方墨點法細胞準備 37 2.4.4壓力及超音波刺激調控髓核細胞Sox9實驗 38 2.5髓核細胞靜水壓與超音波刺激實驗討論 42 2.6髓核細胞靜水壓與超音波刺激鈣離子實驗 43 2.6.1髓核細胞靜水壓與超音波刺激鈣離子實驗設計 43 2.6.2鈣離子即時影像細胞式樣準備 44 2.6.3鈣離子螢光染色影像 44 2.6.4髓核細胞靜水壓與超音波刺激鈣離子實驗結果 45 2.7髓核細胞靜水壓刺激鈣離子調控路徑實驗 49 2.7.1使用藥品 49 2.7.2實驗流程 50 2.7.3髓核細胞靜水壓刺激鈣離子調控路徑實驗結果 50 2.8壓力刺激鈣離子FURA2影像實驗 59 2.8.1 Fura2實驗細胞準備 59 2.7.2鈣離子Fura2螢光染色影像 60 2.8.2鈣離子Fura2結果 60 2.9壓力刺激鈣離子影像實驗討論 62 第三章、超音波刺激對髓核細胞之調控 64 3.1超音波刺激髓核細胞鈣離子即時影像之實驗準備 64 3.1.1鈣離子即時影像細胞式樣準備 64 3.1.2鈣離子螢光染色影像 65 3.1.3細胞刺激影像載台(Live Imaging Chamber, LIC) 65 3.1.4玻璃針尖(Micropipette)超音波刺激設備 66 3.1.5玻璃針尖(Micropipette)超音波能量校正 67 3.2超音波玻璃針尖刺激之鈣離子實驗 69 3.3超音波調控髓核細胞鈣離子路徑實驗 71 3.3.1使用藥品 71 3.3.2實驗流程 72 3.4超音波玻璃針尖刺激之鈣離子實驗討論 78 第四章、結論 80 參考文獻 80 | |
| dc.language.iso | zh-TW | |
| dc.subject | 靜水壓 | zh_TW |
| dc.subject | 椎間盤退化 | zh_TW |
| dc.subject | 下背痛 | zh_TW |
| dc.subject | 髓核細胞 | zh_TW |
| dc.subject | 超音波 | zh_TW |
| dc.subject | nucleus pulposus | en |
| dc.subject | hydrostatic pressure | en |
| dc.subject | Low back pain | en |
| dc.subject | ultrasound | en |
| dc.subject | intervertebral disc | en |
| dc.title | 髓核細胞對外力刺激的反應 | zh_TW |
| dc.title | The response of nucleus pulposus to external force stimulation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林頌然(Sung-Jan Lin),劉浩澧(Hao-Li Liu),賴伯亮(Po-Liang Lai) | |
| dc.subject.keyword | 下背痛,椎間盤退化,髓核細胞,超音波,靜水壓, | zh_TW |
| dc.subject.keyword | Low back pain,intervertebral disc,nucleus pulposus,ultrasound,hydrostatic pressure, | en |
| dc.relation.page | 83 | |
| dc.identifier.doi | 10.6342/NTU202100703 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2021-02-18 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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