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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡向榮(Hsiang-Jung Tsai) | |
dc.contributor.author | Yi-Jiun Lin | en |
dc.contributor.author | 林怡君 | zh_TW |
dc.date.accessioned | 2021-05-17T09:22:20Z | - |
dc.date.available | 2014-02-16 | |
dc.date.available | 2021-05-17T09:22:20Z | - |
dc.date.copyright | 2012-02-16 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-02-01 | |
dc.identifier.citation | 王芃宣,2005。台灣地區鵝環狀病毒與鴨環狀病毒之PCR檢測、基因型分析與宿主特異性研究。國立中興大學碩士論文,台中。
王羣,2006。豬環狀病毒之概述。行政院農業委員會家畜衛生試驗所。 http://vettech.nvri.gov.tw/Articles/ahm/334.html 許志明,2006。APV及PBFDV的分子分析與鸚鵡APV酵素免疫分析套組之開發。國立台灣大學碩士論文,台北。 Abadie, J., Nguyen, F., Groizeleau, C., Amenna, N., Fernandez, B., Guereaud, C., Guigand, L., Robart, P., Lefebvre, B., Wyers, M., 2001, Pigeon circovirus infection: pathological observations and suggested pathogenesis. Avian Pathol 30, 149-158. Adair, B.M., 2000, Immunopathogenesis of chicken anemia virus infection. Dev Comp Immunol 24, 247-255. Allan, G.M., Ellis, J.A., 2000, Porcine circoviruses: a review. J Vet Diagn Invest 12, 3-14. Allan, G.M., Mc Neilly, F., Meehan, B.M., Kennedy, S., Mackie, D.P., Ellis, J.A., Clark, E.G., Espuna, E., Saubi, N., Riera, P., Botner, A., Charreyre, C.E., 1999, Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 66, 115-123. Allan, G.M., Phenix, K.V., Todd, D., McNulty, M.S., 1994, Some biological and physico-chemical properties of porcine circovirus. Zentralbl Veterinarmed B 41, 17-26. Bassami, M.R., Berryman, D., Wilcox, G.E., Raidal, S.R., 1998, Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. Virology 249, 453-459. Biagini, P., Uch, R., Belhouchet, M., Attoui, H., Cantaloube, J.F., Brisbarre, N., de Micco, P., 2007, Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach. J Gen Virol 88, 2696-2701. Bougiouklis, P.A., 2007, Avian circoviruses of the genus Circovirus: A potential trigger in Pigeon breeder's lung (PBL)/Bird fancier's lung (BFL). Med Hypotheses 68, 320-323. Chen, C.L., Chang, P.C., Lee, M.S., Shien, J.H., Ou, S.J., Shieh, H.K., 2003, Nucleotide sequences of goose circovirus isolated in Taiwan. Avian Pathol 32, 165-171. Cho, H.S., Kim, T.J., Lee, J.I., Park, N.Y., 2006, Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2. Can J Vet Res 70, 263-268. Claessens, J.A., Schrier, C.C., Mockett, A.P., Jagt, E.H., Sondermeijer, P.J., 1991, Molecular cloning and sequence analysis of the genome of chicken anaemia agent. J Gen Virol 72 ( Pt 8), 2003-2006. Clark, E.G., 1997, Post-weaing multisystemic wasting syndrome. Proceedings of the American Association for Swine Practices 28: 499-501. Crowther, R.A., Berriman, J.A., Curran, W.L., Allan, G.M., Todd, D., 2003, Comparison of the structures of three circoviruses: chicken anemia virus, porcine circovirus type 2, and beak and feather disease virus. J Virol 77, 13036-13041. Cságola, A., Lorincz, M., Tombacz, K., Wladar, Z., Kovacs, E., Tuboly, T., 2011, Genetic diversity of pigeon circovirus in Hungary. Virus Genes. Daum, I., Finsterbusch, T., Hartle, S., Gobel, T., Mankertz, A., Korbel, R., Grund, C., 2009, Cloning and expression of a truncated pigeon circovirus capsid protein suitable for antibody detection in infected pigeons. Avian Pathol 38, 135-141. De Boer, G.F., Van Roozelaar, D.J., Moormann, R.J., Jeurissen, S.H., Wijngaard, J.C., Hilbink, F., Koch, G., 1994, Interaction between chicken anaemia virus and live Newcastle disease vaccine. Avian Pathol 23, 263-275. Dean, F.B., Nelson, J.R., Giesler, T.L., Lasken, R.S., 2001, Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res 11, 1095-1099. Duchatel, J.P., Jauniaux, T., Smyth, J., Habsch, I., de Bournonville, M., Losson, B., Todd, D., 2010, Effect of a commercial paratyphus vaccine on the development of pigeon circovirus infection in young pigeons (Columba livia domestica). J Avian Med Surg 24, 107-114. Duchatel, J.P., Todd, D., Curry, A., Smyth, J.A., Bustin, J.C., Vindevogel, H., 2005, New data on the transmission of pigeon circovirus. Vet Rec 157, 413-415. Duchatel, J.P., Todd, D., Smyth, J.A., Bustin, J.C., Vindevogel, H., 2006, Observations on detection, excretion and transmission of pigeon circovirus in adult, young and embryonic pigeons. Avian Pathol 35, 30-34. Duchatel, J.P., Todd, D., Willeman, C., Losson, B., 2009, Quantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reaction. Avian Pathol 38, 143-148. Franciosini, M.P., Fringuelli, E., Tarhuni, O., Guelfi, G., Todd, D., Casagrande Proietti, P., Falocci, N., Asdrubali, G., 2005, Development of a polymerase chain reaction-based in vivo method in the diagnosis of subclinical pigeon circovirus infection. Avian Dis 49, 340-343. Freick, M., Muller, H., Raue, R., 2008, Rapid detection of pigeon herpesvirus, fowl adenovirus and pigeon circovirus in young racing pigeons by multiplex PCR. J Virol Methods 148, 226-231. Gelderblom, H., Kling, S., Lurz, R., Tischer, I., von Bulow, V., 1989, Morphological characterization of chicken anaemia agent (CAA). Arch Virol 109, 115-120. Goldsmith, T.L., 1995, Documentation of passerine circoviral infection. Proceeding of Association for Avian Veterinary 16:349. Gough, R.E., Drury, S.E., 1996, Circovirus-like particles in the bursae of young racing pigeons. Vet Rec 138, 167. Halami, M.Y., Nieper, H., Muller, H., Johne, R., 2008, Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Virus Res 132, 208-212. Hattermann, K., Schmitt, C., Soike, D., Mankertz, A., 2003, Cloning and sequencing of Duck circovirus (DuCV). Arch Virol 148, 2471-2480. Hattermann, K., Soike, D., Grund, C., Mankertz, A., 2002, A method to diagnose Pigeon circovirus infection in vivo. J Virol Methods 104, 55-58. Heath, L., Martin, D.P., Warburton, L., Perrin, M., Horsfield, W., Kingsley, C., Rybicki, E.P., Williamson, A.L., 2004, Evidence of unique genotypes of beak and feather disease virus in southern Africa. J Virol 78, 9277-9284. Islam, M.R., Johne, R., Raue, R., Todd, D., Muller, H., 2002, Sequence analysis of the full-length cloned DNA of a chicken anaemia virus (CAV) strain from Bangladesh: evidence for genetic grouping of CAV strains based on the deduced VP1 amino acid sequences. J Vet Med B Infect Dis Vet Public Health 49, 332-337. Johne, R., Fernandez-de-Luco, D., Hofle, U., Muller, H., 2006, Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. J Gen Virol 87, 1189-1195. Koch, G., van Roozelaar, D.J., Verschueren, C.A., van der Eb, A.J., Noteborn, M.H., 1995, Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13, 763-770. Li, L., Kapoor, A., Slikas, B., Bamidele, O.S., Wang, C., Shaukat, S., Masroor, M.A., Wilson, M.L., Ndjango, J.B., Peeters, M., Gross-Camp, N.D., Muller, M.N., Hahn, B.H., Wolfe, N.D., Triki, H., Bartkus, J., Zaidi, S.Z., Delwart, E., 2010, Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. J Virol 84, 1674-1682. Li, L., Shan, T., Soji, O.B., Alam, M.M., Kunz, T.H., Zaidi, S.Z., Delwart, E., 2011, Possible cross-species transmission of circoviruses and cycloviruses among farm animals. J Gen Virol 92, 768-772. Liu, C., Ihara, T., Nunoya, T., Ueda, S., 2004, Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen. J Vet Med Sci 66, 237-242. Liu, S.-N., Zhang, X.-X., Zou, J.-F., Xie, Z.-J., Zhu, Y.-L., Zhao, Q., Zhou, E.-m., Jiang, S.-J., 2010, Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks. Vet Microbiol 145, 41-46. Mankertz, A., Hattermann, K., Ehlers, B., Soike, D., 2000, Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons. Arch Virol 145, 2469-2479. Mankertz, A., Persson, F., Mankertz, J., Blaess, G., Buhk, H.J., 1997, Mapping and characterization of the origin of DNA replication of porcine circovirus. J Virol 71, 2562-2566. Marlier, D., Vindevogel, H., 2006, Viral infections in pigeons. Vet J 172, 40-51. McFerran, J.B., McNulty, M.S., Curran, W.L., 1978, Diagnosis of avian viral diseass by electron microscopy. Am J Vet Res 39, 505-508. McNicol, A.M., Farquharson, M.A., 1997, In situ hybridization and its diagnostic applications in pathology. J Pathol 182,250-261. McNulty, M.S., 1991, Chicken anaemia agent: a review. Avian Pathol 20, 187-203. Meehan, B.M., Creelan, J.L., McNulty, M.S., Todd, D., 1997, Sequence of porcine circovirus DNA: affinities with plant circoviruses. J Gen Virol 78 ( Pt 1), 221-227. Meehan, B.M., McNeilly, F., Todd, D., Kennedy, S., Jewhurst, V.A., Ellis, J.A., Hassard, L.E., Clark, E.G., Haines, D.M., Allan, G.M., 1998, Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol 79 ( Pt 9), 2171-2179. Miyata, H., Tsunoda, H., Kazi, A., Yamada, A., Khan, M.A., Murakami, J., Kamahora, T., Shiraki, K., Hino, S., 1999, Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. J Virol 73, 3582-3586. Mushahwar, I.K., Erker, J.C., Muerhoff, A.S., Leary, T.P., Simons, J.N., Birkenmeyer, L.G., Chalmers, M.L., Pilot-Matias, T.J., Dexai, S.M., 1999, Molecular and biophysical characterization of TT virus: evidence for a new virus family infecting humans. Proc Natl Acad Sci U S A 96, 3177-3182. Mysore, J., Read, D., Daft, B., 1995, Circovirus-like particles in finches (abstract). In Proceedings of the Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians, Histopathology Section,Reno, NV, USA, p.2. Nawagitgul, P., Harms, P.A., Morozov, I., Thacker, B.J., Sorden, S.D., Lekcharoensuk, C., Paul, P.S., 2002, Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immunol 9, 33-40. Niagro, F.D., Forsthoefel, A.N., Lawther, R.P., Kamalanathan, L., Ritchie, B.W., Latimer, K.S., Lukert, P.D., 1998, Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Arch Virol 143, 1723-1744. Nishizawa, T., Okamoto, H., Konishi, K., Yoshizawa, H., Miyakawa, Y., Mayumi, M., 1997, A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun 241, 92-97. Noteborn, M.H., de Boer, G.F., van Roozelaar, D.J., Karreman, C., Kranenburg, O., Vos, J.G., Jeurissen, S.H., Hoeben, R.C., Zantema, A., Koch, G., et al., 1991, Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. J Virol 65, 3131-3139. Noteborn, M.H., Verschueren, C.A., van Ormondt, H., van der Eb, A.J., 1998, Chicken anemia virus strains with a mutated enhancer/promoter region share reduced virus spread and cytopathogenicity. Gene 223, 165-172. Oritiz-Catedral, L., Kurenbach, B., Massaro, M., McInnes, K., Brunton, D.H., Hauber, M.E., Martin, D.P., Varsani, A., 2010, A new isolate of beak and feather disease virus from endemic wild red-fronted parakeets (Cyanoramphus novaezelandiae) in New Zealand. Arch Virol 155(4), 613-620. Pages-Mante, A., Saubi, N., Artigas, C., Espuna, E., 1997, Experimental evaluation of an inactivated vaccine against chicken anaemia virus. Avian Pathol 26, 721-729. Pass, D.A., Perry, R.A., 1984, The pathology of psittacine beak and feather disease. Aust Vet J 61, 69-74. Phenix, K.V., Weston, J.H., Ypelaar, I., Lavazza, A., Smyth, J.A., Todd, D., Wilcox, G.E., Raidal, S.R., 2001, Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. J Gen Virol 82, 2805-2809. Racine, S., Kheyar, A., Gagnon, C.A., Charbonneau, B., Dea, S., 2004, Eucaryotic expression of the nucleocapsid protein gene of porcine circovirus type 2 and use of the protein in an indirect immunofluorescence assay for serological diagnosis of postweaning multisystemic wasting syndrome in pigs. Clin Diagn Lab Immunol 11, 736-741. Raidal, S.R., Cross, G.M., 1994, The haemagglutination spectrum of psittacine beak and feather disease virus. Avian Pathol 23, 621-630. Rampin, T., Manarolla, G., Pisoni, G., Recordati, C., Sironi, G., 2006, Circovirus inclusion bodies in intestinal muscle cells of a canary. Avian Pathol 35, 277-279. Raue, R., Schmidt, V., Freick, M., Reinhardt, B., Johne, R., Kamphausen, L., Kaleta, E., Müller, H., Krautwald-Junghanns, M.-E., 2005, A disease complex associated with pigeon circovirus infection, young pigeon disease syndrome. Avian Pathol 34, 418-425. Rector, A., Tachezy, R., Van Ranst, M., 2004, A sequence-independent strategy for detection and cloning of circular DNA virus genomes by using multiply primed rolling-circle amplification. J Virol 78, 4993-4998. Ritchie, B.W., Niagro, F.D., Lukert, P.D., Steffens, W.L., 3rd, Latimer, K.S., 1989, Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology 171, 83-88. Roy, P., Dhillon, A.S., Lauerman, L., Shivaprasad, H.L., 2003, Detection of pigeon circovirus by polymerase chain reaction. Avian Dis 47, 218-222. Schmidt, V., Schlomer, J., Luken, C., Johne, R., Biere, B., Muller, H., Krautwald-Junghanns, M.E., 2008, Experimental infection of domestic pigeons with pigeon circovirus. Avian Dis 52, 380-386. Scott, A.N., Beckett, A., Smyth, J.A., Ball, N.W., Palya, V., Todd, D., 2006, Serological diagnosis of goose circovirus infections. Avian Pathol 35, 495-499. Scullion, F.T., Scullion, M.G., 2007, Pathologic findings in racing pigeons (Columba livia domestica) with 'young bird sickness'. J Avian Med Surg 21, 1-7. Shivaprasad, H.L., Chin, R.P., Jeffrey, J.S., Latimer, K.S., Nordhausen, R.W., Niagro, F.D., Campagnoli, R.P., 1994, Particles resembling circovirus in the bursa of Fabricius of pigeons. Avian Dis 38, 635-641. Shivaprasad, H.L., Hill, D., Todd, D., Smyth, J.A., 2004, Circovirus infection in a Gouldian finch (Chloebia gouldiae). Avian Pathol 33, 525-529. Smyth, J., Soike, D., Moffett, D., Weston, J.H., Todd, D., 2005, Circovirus-infected geese studied by in situ hybridization. Avian Pathol 34, 227-232. Smyth, J.A., Carroll, B.P., 1995, Circovirus infection in European racing pigeons. Vet Rec 136, 173-174. Smyth, J., Todd, D., Scott, A.N., Twentman, C.M., Gavier-Widen, D., 2006, Identification of circovirus infection in three species of gull. Vet Rec 159 (7): 212-214. Smyth, J.A., Weston, J., Moffett, D.A., Todd, D., 2001, Detection of circovirus infection in pigeons by in situ hybridization using cloned DNA probes. J Vet Diagn Invest 13, 475-482. Soike, D., 1997, Circovirus infections in pigeons. Tierarztl Prax 25, 52-54. Soike, D., Hattermann, K., Albrecht, K., Segales, J., Domingo, M., Schmitt, C., Mankertz, A., 2001, A diagnostic study on columbid circovirus infection. Avian Pathol 30, 605-611. Soike, D., Kohler, B., Albrevht, K., 1999, A circovirus-like infection of geese related to a runting syndrome. Avian Pathol, 28:199-202. Steenhuisen, W., Jagt, HJM., Schrier, C.C., 1994, The use od a live attenuated CAV vaccine in breeder flocks in the Netherlands. Proceeding of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Rauischolzhausen, Germany: University of Giesen, pp. 482-497. Stewart, M.E., Perry, R., Raidal, S.R., 2006, Identification of a novel circovirus in Australian ravens (Corvus coronoides) with feather disease. Avian Pathol 35, 86-92. Takahashi, K., Iwasa, Y., Hijikata, M., Mishiro, S., 2000, Identification of a new human DNA virus (TTV-like mini virus, TLMV) intermediately related to TT virus and chicken anemia virus. Arch Virol 145, 979-993. Tischer, I., Rasch, R., Tochtermann, G., 1974, Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Orig A 226, 153-167. Todd, D., 2000, Circoviruses: Immunosuppressive threats to avian species: A review. Avian Pathol 29, 373-394. Todd, D., 2001a, Genome Sequence Determinations and Analyses of Novel Circoviruses from Goose and Pigeon. Virology 286, 354-362. Todd, D., 2004, Avian circovirus diseases: lessons for the study of PMWS. Vet Microbiol 98, 169-174. Todd, D., Duchatel, J.P., Bustin, J.C., Scullion, F.T., Scullion, M.G., Scott, A.N., Curry, A., Ball, N.W., Smyth, J.A., 2006, Detection of pigeon circovirus in cloacal swabs: implications for diagnosis, epidemiology and control. Vet Rec 159, 314-317. Todd, D., Duchatel, J.P., Weston, J.H., Ball, N.W., Borghmans, B.J., Moffett, D.A., Smyth, J.A., 2002, Evaluation of polymerase chain reaction and dot blot hybridisation tests in the diagnosis of pigeon circovirus infections. Vet Microbiol 89, 1-16. Todd, D., Fringuelli, E., Scott, A., Borghmans, B., Duchatel, J., Shivaprasad, H., Raidal, S., Abadie, J., Franciosini, M., Smyth, J., 2008, Sequence comparison of pigeon circoviruses. Res Vet Sci 84, 311-319. Todd, D., Niagro, F.D., Ritchie, B.W., Curran, W., Allan, G.M., Lukert, P.D., Latimer, K.S., Steffens, W.L., 3rd, McNulty, M.S., 1991, Comparison of three animal viruses with circular single-stranded DNA genomes. Arch Virol 117, 129-135. Todd, D., Scott, A.N., Fringuelli, E., Shivraprasad, H.L., Gavier-Widen, D., Smyth, J.A., 2007, Molecular characterization of novel circoviruses from finch and gull. Avian Pathol 36, 75-81. Todd, D., Weston, J., Ball, N.W., Borghmans, B.J., Smyth, J.A., Gelmini, L., Lavazza, A., 2001b, Nucleotide sequence-based identification of a novel circovirus of canaries. Avian Pathol 30, 321-325. Uzun, O., Yilmaz, D.K., Sunter, A.T., Erkan, L., 2011, The Prevalence of “Pigeon Breeder’s Disease” in a Turkish City and Review of the Literature. Lung. Vielitz, E., Voss, M., 1994, Experiences with a commercial CAV-vaccine. Proceeding of the International Symposium on Infectious Bursal Disease and Infectious Chicken Anaemia, Rauischolzhausen, Germany: University of Giesen, pp. 465-481. Woods, L.W., Latimer, K.S., Barr, B.C., Niagro, F.D., Campagnoli, R.P., Nordhausen, R.W., Castro, A.E., 1993, Circovirus-like infection in a pigeon. J Vet Diagn Invest 5, 609-612. Woods, L.W., Latimer, K.S., Niagro, F.D., Riddell, C., Crowley, A.M., Anderson, M.L., Daft, B.M., Moore, J.D., Campagnoli, R.P., Nordhausen, R.W., 1994, A retrospective study of circovirus infection in pigeons: nine cases (1986-1993). J Vet Diagn Invest 6, 156-164. Woods, L.W., Latimer, K.S., 2000, Circovirus infection of nonpsittacine birds. J Avian Med Surg 14(3), 154-163. Yuasa, N., 1992, Effect of chemicals on the infectivity of chicken anaemia virus. Avian Pathol 21, 315-319. Yuasa, N., Tanihuchi, T., Yoshida, I., 1979, Isolation and some characteristics of an agent inducing anemia in chicks. Avian Dis 23: 366-385. Zhang, Z., Lu, C., Wang, Y., Wang, S., Dai, D., Chen, Z., Fan, H., 2011, Molecular characterization and epidemiological investigation of Pigeon circovirus isolated in eastern China. J Vet Diagn Invest 23, 665-672. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6959 | - |
dc.description.abstract | 鴿子環狀病毒 (Pigeon circovirus, PiCV) 為在年輕鴿群中一新浮現性之疾病,並且被認為是幼鴿症候群 (Young pigeon disease syndrome)的重要因素之一。而環狀病毒在燕雀目鳥類 (order Passeriformes) 如金絲雀及其他雀科鳥類等所扮演的角色鮮少有報告,目前在台灣燕雀目鳥類為相當常見之鳥種,但並無相關環狀病毒盛行率之報告。此篇研究主要是調查台灣地區鴿子及燕雀目鳥類環狀病毒的盛行率及其親緣性分析,PiCV檢測部分自2009-2011年共蒐集台灣北部及中部共56個鴿舍736隻賽鴿及57隻種鴿之共泄腔拭子與全台391隻野鴿之糞便拭子,鴿舍之陽性率為 94.6 % (53/56),賽鴿及種鴿之檢測陽性率分別為 68.5 % (504/736) 及38.6 % (22/57),且PiCV陽性率與賽鴿具臨床症狀如下痢、食慾不振、體重下降或飛行表現不佳等呈極顯著之差異(p<0.01);而野鴿之檢測陽性率為31%,且各地區之陽性率差異不大。將部分核酸產物經定序後,與基因庫 (GenBank) 中不同國家之PiCV序列比對,ORF V1之核酸序列及胺基酸序列比對結果相似度為94.3-99.6%及87.1-100 %,而另一個ORF C1與基因庫之比對結果相似度分別為74.2-99.2 %及73.2-99.8 %,且此台灣分離株與比利時株較接近,顯示台灣地區之鴿子可能由國外引進不顯性感染PiCV之健康鴿,並造成大規模之病毒傳播。另利用原位雜交法可見病毒核酸標記在肝、腎及肺的細胞質中。而在寵物鳥店之鳥類自2010-2011年共蒐集18場310隻鳥類之糞便拭子,寵物鳥店之環狀病毒陽性率為 88.9 % (16/18),而其環狀病毒盛行率為54.8 % (170/310),以燕雀目鳥類之綠繡眼陽性率最高為 91.9 % (102/111)。將ORF V1之部分核酸產物經定序後,與基因庫 (GenBank) 之不同鳥種之序列比對,其核酸序列相似性百分比為61.2-100 %,依其演化的距離及相異度可區分為4個分支,分別與Psittacine beak and feather disease virus (PBFDV), PiCV, Finch circovirus (FiCV) 及 Raven circovirus (RaCV) 較接近;將與PiCV分支相近之ORF C1與和GenBank中不同國家之鴿子環狀病毒分離株核酸序列及胺基酸序列相比,可得相似性百分比為77.2-99.6 %及76.4-100 %,顯示燕雀目鳥類可被跨鳥種之環狀病毒感染的可能性。由此研究可得知,環狀病毒藉由糞便水平傳播在台灣的鴿子及常見的燕雀目鳥類之間有高的盛行率,且由核酸序列、胺基酸序列及親緣性分析可得其病毒來源相當複雜。 | zh_TW |
dc.description.abstract | Circovirus infection is an emerging viral disease of young pigeons and maybe a crucial factor of young pigeon disease syndrome. In addition, little is known about the roles of circoviruses in causing disease in passerine species such as canaries and finches. The order Passeriformes are the most common birds in Taiwan, however there has no relevant studies about the prevalence of circovirus in these species. The aims of this study were to investigate the prevalence and the genetic features of the circovirus strains that infecting the pigeon and birds of the order Passeriformes. In this study, cloacal swabs were collected from 736 racing pigeons and 57 breeder pigeons of 56 lofts in northern and central Taiwan, and also fecal swabs were collected from 391 wild pigeons in Taiwan from 2009-2011. 53 out of 56 lofts were positive and the prevalence of pigeon circovirus (PiCV) were 68.5% (504/736) and 38.6% (22/57), respectively. Racing pigeons with clinical signs such as diarrhea, anorexia, weight loss and poor race performance were related to positive percentage (p<0.01). The prevalence of PiCV in wild pigeons were 31% (121/391) with little difference in 4 areas of Taiwan. PiCVs indicated 94.3-99.6 % and 89.1-100 % of nucleotide and amino acid identity in ORF V1, and ORF C1 sequences were 74.2-99.2 % and 73.2-99.8 %, respectively. Phylogenetic analysis of capsid protein genes of the Taiwanese strains were closely related to Belgian strain, which is probably due to the fact that the PiCV-carrying healthy pigeons introduced from abroad could most likely be the origin of the virus and spread to pigeons in Taiwan. The results of in situ hybridization were found mostly in the cytoplasm of infected cells in liver, kidney and lung. Moreover, fecal swabs from 310 birds were collected in pet bird shops in Taiwan from 2010-2011, and the prevalence of circovirus in these birds were 54.8 % (170/310). The highest positive rate was Zosterops japonicas with 91.9 % (102/111). The ORF V1 sequences revealed these viruses were classified in Psittacine beak and feather disease virus (PBFDV), PiCV, Finch circovirus (FiCV) and Raven circovirus (RaCV). The ORF C1 related to PiCV compared with GenBank indicated 77.2%-99.6% and 76.4-100 % of nucleotide and amino acid identity, which showed the possibility of cross-species transmission of circoviruses among order Passeriformes. The results of this study indicated that the PiCV-positive rate was high in the pigeon and order Passeriformes populations by horizontal transmission of fecal in Taiwan, and according to the sequence diversity and phylogenetic trees indicated the complex sources from nature or others. | en |
dc.description.provenance | Made available in DSpace on 2021-05-17T09:22:20Z (GMT). No. of bitstreams: 1 ntu-101-R98629034-1.pdf: 1385017 bytes, checksum: 6c7ad2b4caf6fb60449941cb6b242a07 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 致謝------------------------------------------------------------------I
中文摘要-------------------------------------------------------------II 英文摘要------------------------------------------------------------IV 目錄----------------------------------------------------------------VI 圖錄-----------------------------------------------------------------X 表目錄-------------------------------------------------------------XIII 第一章 緒言--------------------------------------------------------1 第二章 文獻探討----------------------------------------------------3 第一節 環狀病毒之歷史背景----------------------------------------3 第二節 環狀病毒之分類與特徵--------------------------------------5 2.1 環狀病毒之分類----------------------------------------------5 2.2 病毒結構----------------------------------------------------5 2.3 病毒基因----------------------------------------------------6 2.4 病毒之物化特性---------------------------------------------10 第三節 環狀病毒之致病性-----------------------------------------11 3.1 PCV之致病性-----------------------------------------------11 3.2 CAV之致病性-----------------------------------------------11 3.3 PBFDV之致病性--------------------------------------------12 3.4 PiCV之致病性----------------------------------------------12 3.5 CaCV之致病性----------------------------------------------12 3.6 FiCV之致病性----------------------------------------------13 3.7 RaCV 之致病性---------------------------------------------13 第四節 環狀病毒之實驗室診斷-------------------------------------13 4.1 以細胞培養分離病毒-----------------------------------------13 4.2 直接觀察病毒-----------------------------------------------14 4.3 檢測病毒核酸-----------------------------------------------14 4.3.1 原位雜交法 (In situ hybridization) --------------------------14 4.3.2 點漬雜交法 (Dot blot hybridization) ------------------------15 4.3.3 聚合酶連鎖反應 (Polymerase chain reaction) -----------------15 4.3.4 多引子滾環式增幅法 (Multiply primed rolling-circle amplification) ------------------------------------------------------16 4.3.4-1 原理簡介-------------------------------------------16 4.3.4-2 RCA於訊號放大之運用------------------------------16 4.4 由血清抗體證實感染-----------------------------------------17 4.4.1 免疫組織化學染色法 (Immunohistochemistry) ---------------17 4.4.2 酵素結合免疫吸附法 (ELISA) ----------------------------18 第五節 環狀病毒之感染控制---------------------------------------18 第六節 環狀病毒之流行病學---------------------------------------19 第七節 幼鴿症候群 (Young pigeon disease syndrome;YPDS) -----------21 第三章 材料與方法-------------------------------------------------23 第一節 試驗材料-------------------------------------------------23 1.1 鴿子糞便樣本-----------------------------------------------23 1.2 鴿子病材---------------------------------------------------24 1.3 寵物鳥店之鳥類糞便樣本-------------------------------------24 1.4 燕雀目鳥類病材---------------------------------------------24 1.5 糞便樣本之保存---------------------------------------------25 1.6 組織病材均質液之製作及保存---------------------------------25 第二節 病毒核酸之萃取-------------------------------------------26 第三節 聚合酶連鎖反應-------------------------------------------26 3.1 引子 (primer) 之選用----------------------------------------26 3.2 聚合酶連鎖反應---------------------------------------------28 3.3 巢式聚合酶連鎖反應-----------------------------------------28 3.4 瓊脂醣凝膠電泳---------------------------------------------29 第四節 多引子滾環式增幅法---------------------------------------29 4.1 鴿子環狀病毒之Multiply primed RCA---------------------------29 4.2 鴿子環狀病毒之限制酶分析-----------------------------------30 第五節 RCA增幅之DNA產物之分子選殖及純化----------------------30 5.1 鴿子環狀病毒之DNA產物純化--------------------------------30 5.2 DNA接合反應----------------------------------------------31 5.3 重組質體之轉型作用-----------------------------------------31 5.4 質體DNA之抽取--------------------------------------------32 5.5 質體DNA之限制酶切割確認----------------------------------33 第六節 核酸序列定序與分析比較及演化分析--------------------------33 6.1 核酸序列之定序---------------------------------------------33 6.2 病毒核酸序列之分析-----------------------------------------34 第七節 原位雜交法 (In situ hybridization) ---------------------------34 7.1 探針之製備-----------------------------------------------34 7.2 原位雜交之操作方法----------------------------------------35 第四章 結果-------------------------------------------------------37 第一節 聚合酶連鎖反應檢測結果-----------------------------------37 1.1 鴿子環狀病毒之陽性率--------------------------------------37 1.2 寵物鳥店鳥類環狀病毒之陽性率------------------------------39 第二節 環狀病毒之基因分析---------------------------------------40 2.1 核酸序列及胺基酸序列之相似度----------------------------40 2.1.1 鴿子環狀病毒 ORF V1 基因-------------------------------40 2.1.2 鴿子環狀病毒 ORF C1 基因-------------------------------41 2.1.3 燕雀目鳥類之環狀病毒ORF V1 基因------------------------42 2.1.4 燕雀目鳥類之環狀病毒ORF C1 基因------------------------43 2.2 環狀病毒親緣樹狀圖----------------------------------------43 2.2.1 鴿子環狀病毒 ORF V1 基因-------------------------------44 2.2.2 鴿子環狀病毒 ORF C1 基因-------------------------------44 2.2.3 燕雀目鳥類之環狀病毒ORF V1 基因-----------------------44 2.2.4 燕雀目鳥類之環狀病毒ORF C1 基因------------------------45 第三節 鴿子及燕雀目鳥類病例-------------------------------------46 3.1 聚合酶連鎖反應--------------------------------------------46 3.2 原位雜交法 (In situ hybridization) -----------------------------46 3.2.1 探針製備-----------------------------------------------46 3.2.2 病毒核酸之偵測-----------------------------------------46 第四節 鴿子環狀病毒之質體DNA----------------------------------47 第五章 討論-------------------------------------------------------48 第一節 陽性率之討論---------------------------------------------48 第二節 診斷方法之討論-------------------------------------------51 第三節 基因序列與親緣性分析之討論-------------------------------53 第六章 參考文獻---------------------------------------------------77 | |
dc.language.iso | zh-TW | |
dc.title | 台灣地區鴿子及燕雀目鳥類環狀病毒流行病學研究 | zh_TW |
dc.title | Epidemiological Analysis of Circovirus Isolated from
Pigeon and the order Passeriformes in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張紹光,周崇熙,沈瑞鴻,蔡信雄 | |
dc.subject.keyword | 鴿子環狀病毒,幼鴿症候群,燕雀目鳥類,多引子滾環式增幅法,原位雜交法, | zh_TW |
dc.subject.keyword | Pigeon circovirus,young pigeon disease syndrome,order Passeriformes,multiply primed rolling-circle amplification,in situ hybridization, | en |
dc.relation.page | 85 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2012-02-02 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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