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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮(Hsu-Liang Hsieh) | |
dc.contributor.author | Shao-Li Yang | en |
dc.contributor.author | 楊紹立 | zh_TW |
dc.date.accessioned | 2021-06-17T03:14:11Z | - |
dc.date.available | 2019-08-06 | |
dc.date.copyright | 2018-08-06 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-07-11 | |
dc.identifier.citation | Aoyama, T. and Chua, N.H. (1997). A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11: 605–612.
Bennett, T., van den Toorn, A., Sanchez-Perez, G.F., Campilho, A., Willemsen, V., Snel, B., and Scheres, B. (2010). SOMBRERO, BEARSKIN1, and BEARSKIN2 regulate root cap maturation in Arabidopsis. Plant Cell 22: 640–654. Bu, Q., Jiang, H., Li, C.B., Zhai, Q., Zhang, J., Wu, X., Sun, J., Xie, Q., and Li, C. (2008). Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses. Cell Res. 18: 756–767. Casal, J.J., Candia, A.N., and Sellaro, R. (2014). Light perception and signalling by phytochrome A. J. Exp. Bot. 65: 2835–2845. Chen, C.Y., Ho, S.-S., Kuo, T.-Y., Hsieh, H.-L., and Cheng, Y.-S. (2017). Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation. Proc. Natl. Acad. Sci. 114: E1815–E1824. Chen, H.J., Chen, C.L., and Hsieh, H.L. (2015). Far-red light-mediated seedling development in Arabidopsis involves FAR-RED INSENSITIVE 219/JASMONATE RESISTANT 1-dependent and -independent pathways. PLoS One 10: e0132723. Chen, I.-C., Huang, I.-C., Liu, M.-J., Wang, Z.-G., Chung, S.-S., and Hsieh, H.-L. (2007). Glutathione S-Transferase interacting with Far-Red Insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis. Plant Physiol. 143: 1189–1202. Clough, S.J. and Bent, A.F. (1998). Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16: 735–743. Elmayan, T. and Vaucheret, H. (1996). Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally. Plant J. 9: 787–797. Fendrych, M., Van Hautegem, T., Van Durme, M., Olvera-Carrillo, Y., Huysmans, M., Karimi, M., Lippens, S., Guérin, C.J., Krebs, M., Schumacher, K., and Nowack, M.K. (2014). Programmed cell death controlled by ANAC033/SOMBRERO determines root cap organ size in arabidopsis. Curr. Biol. 24: 931–940. van Gelderen, K., Kang, C., Paalman, R., Keuskamp, D.H., Hayes, S., and Pierik, R. (2018). Far-red light detection in the shoot regulates lateral root development through the HY5 transcription factor. Plant Cell 30: 101–116. Hellens, R.P., Allan, A.C., Friel, E.N., Bolitho, K., Grafton, K., Templeton, M.D., Karunairetnam, S., Gleave, A.P., and Laing, W.A. (2005). Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1: 1–14. Hiltbrunner, A., Viczián, A., Bury, E., Tscheuschler, A., Kircher, S., Tóth, R., Honsberger, A., Nagy, F., Fankhauser, C., and Schäfer, E. (2005). Nuclear accumulation of the phytochrome A photoreceptor requires FHY1. Curr. Biol. 15: 2125–2130. Hsieh, H.L., Okamoto, H., Wang, M., Ang, L.H., Matsui, M., Goodman, H., and Deng, X.W. (2000). FIN219, an auxin-regulated gene, defines a link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development. Genes Dev. 14: 1958–1970. Jenkins, G.I. (2014). Structure and function of the UV-B photoreceptor UVR8. Plant Cell 26: 21–37. Jensen, M.K., Kjaersgaard, T., Petersen, K., and Skriver, K. (2010). NAC genes: Time-specific regulators of hormonal signaling in Arabidopsis. Plant Signal. Behav. 5: 907–910. Jensen, M.K. and Skriver, K. (2014). NAC transcription factor gene regulatory and protein-protein interaction networks in plant stress responses and senescence. IUBMB Life 66: 156–166. Jin, J., Tian, F., Yang, D.C., Meng, Y.Q., Kong, L., Luo, J., and Gao, G. (2017). PlantTFDB 4.0: Toward a central hub for transcription factors and regulatory interactions in plants. Nucleic Acids Res. 45: D1040–D1045. Lee, H.J. et al. (2016). Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots. Sci. Signal. 9: 1–9. Li, T., Jia, K.P., Lian, H.L., Yang, X., Li, L., and Yang, H.Q. (2014). Jasmonic acid enhancement of anthocyanin accumulation is dependent on phytochrome A signaling pathway under far-red light in Arabidopsis. Biochem. Biophys. Res. Commun. 454: 78–83. Matallana-Ramirez, L.P., Rauf, M., Farage-Barhom, S., Dortay, H., Xue, G.P., Dröge-Laser, W., Lers, A., Balazadeh, S., and Mueller-Roeber, B. (2013). NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis. Mol. Plant 6: 1438–1452. Möglich, A., Yang, X., Ayers, R.A., and Moffat, K. (2010). Structure and function of plant photoreceptors. Annu. Rev. Plant Biol. 61: 21–47. Nuruzzaman, M., Sharoni, A.M., and Kikuchi, S. (2013). Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants. Front. Microbiol. 4: 248. Reinbothe, C., Springer, A., Samol, I., and Reinbothe, S. (2009). Plant oxylipins: Role of jasmonic acid during programmed cell death, defence and leaf senescence. FEBS J. 276: 4666–4681. Robson, F., Okamoto, H., Patrick, E., Harris, S.-R., Wasternack, C., Brearley, C., and Turner, J.G. (2010). Jasmonate and phytochrome A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability. Plant Cell 22: 1143–1160. Saslowsky, D.E., Warek, U., and Winkel, B.S.J. (2005). Nuclear localization of flavonoid enzymes in Arabidopsis. J. Biol. Chem. 280: 23735–23740. Sessions, A. et al. (2002). A high-throughput Arabidopsis reverse genetics system. Plant Cell 14: 2985–2994. Shao, H., Wang, H., and Tang, X. (2015). NAC transcription factors in plant multiple abiotic stress responses: progress and prospects. Front. Plant Sci. 6: 902. Souer, E., Van Houwelingen, A., Kloos, D., Mol, J., and Koes, R. (1996). The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell 85: 159–170. Staswick, P.E., Su, W., and Howell, S.H. (1992). Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutant. Proc. Natl. Acad. Sci. U. S. A. 89: 6837–6840. Staswick, P.E., Tiryaki, I., and Rowe, M.L. (2002). Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation. Plant Cell 14: 1405–1415. Sudhakar, P., Latha, P., and Reddy, P.V. (2016). Plant pigments. In Phenotyping Crop Plants for Physiological and Biochemical Traits, pp. 121–127. Sullivan, J.A. and Deng, X.W. (2003). From seed to seed: The role of photoreceptors in Arabidopsis development. Dev. Biol. 260: 289–297. Waki, T., Miyashima, S., Nakanishi, M., Ikeda, Y., Hashimoto, T., and Nakajima, K. (2013). A GAL4-based targeted activation tagging system in Arabidopsis thaliana. Plant J. 73: 357–367. Wang, J.G., Chen, C.H., Chien, C.T., and Hsieh, H.L. (2011). FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiol. 156: 631–646. Willemsen, V., Bauch, M., Bennett, T., Campilho, A., Wolkenfelt, H., Xu, J., Haseloff, J., and Scheres, B. (2008). The NAC Domain Transcription Factors FEZ and SOMBRERO Control the Orientation of Cell Division Plane in Arabidopsis Root Stem Cells. Dev. Cell 15: 913–922. Zhou, J., Zhong, R., and Ye, Z.H. (2014). Arabidopsis NAC domain proteins, VND1 to VND5, are transcriptional regulators of secondary wall biosynthesis in vessels. PLoS One 9: e105726. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69378 | - |
dc.description.abstract | 植物演化出各種內在分子機制來適應環境變化。在遠紅光下,光敏素A能被活化,進一步調控植物的生長發育。FIN219/JAR1做為光敏素A的下游,同時參與遠紅光與茉莉酸的訊息傳遞。因此,FIN219/JAR1在兩個訊息傳遞中扮演平衡調節的角色。為了更加了解是否有新的蛋白質調控FIN219/JAR1活性,影響它中介的傳遞路徑,因此,藉由建立fin219-1突變體的基因功能活化突變體種子庫 (activation-tagging mutant pool),在遠紅光中篩選出編號40-3-1植株抑制fin219-1長下胚軸的外表型。40-3-1植株造成幾個遠紅光與茉莉酸相關基因增加表現,更進一步分析發現T-DNA插在SMB啟動子區域造成其大量表現。由雙突變體smb-3 fin219-2分析,SMB在遠紅光訊息傳遞中扮演正調控者的角色,且與FIN219/JAR1位於同一條訊息傳遞路徑。FIN219/JAR1可以干擾SMB形成二聚體,藉此來降低SMB作為轉錄因子的活性。且發現此機制可能在FIN219/JAR1負回饋調控中相當重要。在調控下胚軸延長方面,SMB也具有劑量效應的現象。總合以上所述,根冠專一性表達的轉錄因子SMB可以藉由參與茉莉酸的訊息傳遞,向上影響下胚軸延長,促進遠紅光的光型態發生。因此,推測在正常植物生長階段,茉莉酸的主要來源可能來自於根部,以此調控地上部的發育型態。 | zh_TW |
dc.description.abstract | Plants have several strategies to grow under different environmental changes by adjusting their internal mechanisms. Under far-red (FR) light, phyA is activated to regulate plant growth and development. FIN219/JAR1 acts as a phyA-downstream component and a node of crosstalk with jasmonate (JA) signaling. To further understand functions of FIN219/JAR1 in regulation FR light and JA signaling, an activation-tagging pool of fin219-1 was generated. By selection of extragenic suppressors of fin219-1 under FR light, the 40-3-1 mutant line was obtained with a suppression of fin219-1 long-hypocotyl phenotype and the mutation affected the expression of several light- and JA-responsive genes. Further characterization revealed that the 40-3-1 was caused by SMB overexpression. The assay of smb-3 fin219-2 double mutant suggested that SMB acts in the same pathway as FIN219/JAR1 in FR light and JA signaling. FIN219/JAR1 interrupted SMB dimerization and resulted in the repression of SMB activity. Furthermore, SMB regulated FIN219/JAR1 transcription in a negative feedback manner. SMB also showed a dosage effect on the modulation of hypocotyl elongation. Taken together, the root cap-specific transcription factor SMB regulates JA signaling and further affects hypocotyl elongation in FR-mediated photomorphogenesis, which suggests that the main source of JA in normal development of seedlings might be from root to aboveground tissues to regulate several physiological processes. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T03:14:11Z (GMT). No. of bitstreams: 1 ntu-107-R05b42011-1.pdf: 11483472 bytes, checksum: 21d6835353827df2db915772c03cda83 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書..................................................................................................I
誌謝......................................................................................................................II 中文摘要...............................................................................................................III Abstract...............................................................................................................IV Contents..............................................................................................................VI List of Figures......................................................................................................VIII List of Tables........................................................................................................X Introduction..........................................................................................................1 1. Light and plant development.........................................................................1 2. The role of FIN219, a phyA downstream factor, in FR light signaling.............1 3. The crosstalk of FR light and JA signaling via FIN219/JAR1..........................3 4. Plant specific NAC transcription factors.......................................................4 5. The motivation and purpose of this study.....................................................7 Materials and Methods.........................................................................................9 1. Plant materials...............................................................................................9 2. Plasmid construction and Arabidopsis transformation..................................9 3. Sterilization and sowing of Arabidopsis seeds..............................................10 4. MeJA and light treatment.............................................................................10 5. Measurement of hypocotyl and root lengths.................................................11 6. Measurement of pigment contents...............................................................11 7. Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR).........12 8. Analysis of gene expression..........................................................................13 9. Analysis of protein expressions.....................................................................14 10. Bimolecular fluorescence complementation (BiFC)........................................15 11. Pull-down assay.............................................................................................15 12. Electrophoretic mobility shift assay (EMSA).................................................16 13. Luciferase assay...........................................................................................17 Results..................................................................................................................18 1. The suppressor line 40-3-1 rescues fin219-1 long hypocotyl phenotype.......18 2. The suppressor line 40-3-1 of fin219-1 mutant is due to SMB overexpression. .....................................................................................................................19 3. The expressions of several light- and JA-responsive genes are rescued in line 40-3-1...........................................................................................................21 4. The epistatic relationship between SMB and FN219 in FR light signaling shows a JA-dependent manner................................................................................23 5. The negative feedback of FIN219 is regulated by the protein-protein interaction of SMB and FIN219......................................................................26 6. SMB overexpression promotes JA responses in a FIN219-dependent manner. .....................................................................................................................28 Discussion............................................................................................................30 1. SMB acts a suppressor of FIN219 in modulation of hypocotyl elongation under FR light..........................................................................................................30 2. SMB participates in both FR light and JA signaling.......................................32 3. SMB binds on FIN219 promoter to regulate FIN219, the negative-feedback regulation of which is also SMB-mediated....................................................34 4. SMB overexpression suppresses the hypocotyl phenotype in both fin219-1 and fin219-2, and SMB shows a dosage effect in FR light and JA signaling. ....................................................................................................................36 5. SMB promotes FR light photomorphogenesis in a FIN219-dependent manner. ....................................................................................................................38 Figures.................................................................................................................40 References...........................................................................................................56 Supplemental figures...........................................................................................63 Tables..................................................................................................................72 | |
dc.language.iso | en | |
dc.title | FIN219基因外抑制者SOMBRERO/ANAC033參與遠紅光以及茉莉酸訊息調控下胚軸延長之功能性研究 | zh_TW |
dc.title | Functional studies of SOMBRERO/ANAC033 acting as an extragenic suppressor of FIN219 and regulating hypocotyl elongation of Arabidopsis seedlings in far-red light and jasmonate signaling | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王雅筠(Ya-Yun Wang),孫智雯(Chih-Wen Sun),吳素幸(Shu-Hsing Wu),涂世隆(Shih-Long Tu) | |
dc.subject.keyword | 基因外抑制子,遠紅光光型態發生,FIN219/JAR1,茉莉酸,SMB, | zh_TW |
dc.subject.keyword | extragenic suppressor,far-red light photomorphogenesis,FIN219/JAR1,jasmonate,SMB, | en |
dc.relation.page | 78 | |
dc.identifier.doi | 10.6342/NTU201801425 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-07-11 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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